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Testing Functional Homology Of The Lga2 And Rga2 Genes Of Sporisorium Reilianum In Ustilago Maydis, Joshua Thomas
Testing Functional Homology Of The Lga2 And Rga2 Genes Of Sporisorium Reilianum In Ustilago Maydis, Joshua Thomas
College of Arts & Sciences Senior Honors Theses
Ustilago maydis, a basidiomycete fungus, infects corn and displays dimorphism, transitioning between nonpathogenic and pathogenic stages. Its life cycle involves mating of haploids with different alleles, resulting in the formation of galls/tumors filled with teliospores. The a1 and a2 alleles regulate pheromone recognition and mating. The lga2 and rga2 genes in the a2 allele are crucial for uniparental mitochondrial inheritance. Sporisorium reilianum, a related fungus, has homologs of lga2 and rga2 with U. maydis. To test the functional homology of the S. reilianum genes, protoplasts of U. maydis strains deleted for lga2 or lga2 and rga2 were transformed …
Investigating Pre-Health Students’ Science Identity And The Factors That Influence Them To Change Programs Or Tracks., Taylor Humphreys
Investigating Pre-Health Students’ Science Identity And The Factors That Influence Them To Change Programs Or Tracks., Taylor Humphreys
College of Arts & Sciences Senior Honors Theses
Retention is a particularly pressing issue for undergraduate students, specifically those in pre-health programs or tracks. This can be affected by a students’ science identity, which is referred to as viewing oneself as a “science person.” This study focused on investigating science identity and influencing factors that can cause program or track change of pre-health students. Previous studies have investigated these factors regarding pre-health students applying to medical school, but this study opened the investigation to a variety of pre-health students and focused on characterizing students’ experiences prior to their health professional school applications. The analysis of peer-led focus group …
Sdf-1Α Mediates Primary Tumor Escape In Glioblastoma Through Activation Of Mesenchymal Transitions., Charles T. Froman-Glover
Sdf-1Α Mediates Primary Tumor Escape In Glioblastoma Through Activation Of Mesenchymal Transitions., Charles T. Froman-Glover
College of Arts & Sciences Senior Honors Theses
Glioblastoma (GBM), a highly aggressive primary brain tumor originating in glial cells, poses a significant challenge due to its rapid growth and invasive nature within healthy brain tissue.
Current treatments involve surgical resection, chemotherapy, and radiation. These treatments alone are not enough to cure this disease, and a better understanding the mechanics of the tumor micro-environment is imperative to furthering the field of cancer research. This research focuses on understanding the tumor microenvironment's impact, specifically investigating the role of stromal cell-derived factor 1 (SDF-1) mechanics on GBM aggressiveness. SDF-1 is known to facilitate disease progression by facilitating chemotaxis toward the …
The Anti-Diabetic Drug Metformin Disrupts Feeding And Sleeping Behaviors In Drosophila Melanogaster., Lucas Fitzgerald
The Anti-Diabetic Drug Metformin Disrupts Feeding And Sleeping Behaviors In Drosophila Melanogaster., Lucas Fitzgerald
College of Arts & Sciences Senior Honors Theses
Dimethylbiguanide, also known as metformin, is the single most prescribed oral treatment for non-insulin dependent diabetes mellitus, or type 2 diabetes, in Western countries. The primary mechanism of action that metformin acts through is the activation of AMP kinase, an important regulator of energy homeostasis. While the anti-diabetic effects of metformin are well documented, its effects on feeding and sleeping behaviors are not well characterized. Using the model organism Drosophila melanogaster, the mean daily quantity of food consumed was measured and compared between groups treated with several dosages of metformin. Feeding interactions such as meal frequency and length were …
"Not That Type Of Asian": Deconstructing The Model Minority Myth From A South Asian Perspective., Shraddha Patel
"Not That Type Of Asian": Deconstructing The Model Minority Myth From A South Asian Perspective., Shraddha Patel
College of Arts & Sciences Senior Honors Theses
The myth of the model minority is an essential aspect of the literature on Asian American racialization. However, dominant narratives of the concept largely focus on East Asians, with limited literature highlighting its unique impact on South Asians. In light of this, this qualitative study sought to illuminate where South Asian experiences align with, deviate from, or expand upon the themes observed in existing literature. Using interview data from 21 South Asian American participants from across the United States, we explored 1) identity and sense of belonging, 2) mental health as it pertains to expectations associated with the model minority …
Assessing The Role Of Stim1 And Stim2 In Microglial Response To Spinal Cord Injury., Sarayu Alli
Assessing The Role Of Stim1 And Stim2 In Microglial Response To Spinal Cord Injury., Sarayu Alli
College of Arts & Sciences Senior Honors Theses
Microglia, immune cells of the central nervous system, constantly survey their environment for damage. Once damage is detected, they are activated into become reactive, resulting in morphological changes, such as larger cell bodies, and functional changes, such as releasing inflammatory mediators. The switch from surveying to reactive occurs because the microglia received signals that sense danger in the environment. The release of Ca2+ from intracellular stores is involved in store-operated calcium entry (SOCE), a signaling pathway. Recent studies have shown that calcium release from the intracellular stores is a factor of microglial activation. SOCE is specific to monitoring low calcium …
A Revised Synthesis Of The Cationic Lipids Dmri And Mlri, Benjamin A. Kostic
A Revised Synthesis Of The Cationic Lipids Dmri And Mlri, Benjamin A. Kostic
College of Arts & Sciences Senior Honors Theses
Lipid nanoparticles (LNPs) are an increasingly utilized method of intracellular delivery for nucleic acids. The COVID-19 vaccines from Moderna and Pfizer-BioNTech have used LNP technology to combat the recent global SARS-CoV-2 pandemic, saving millions of lives.1,2 These vaccines consist of a lipid formulation that binds to the anionic phosphate groups of mRNAs and serves as an effective way to transport the nucleic acid across membranes into cells.9The mRNA encodes a viral protein. When introduced to cells, the viral protein is produced so that the immune system then can generate an appropriate antibody response.3 However, this approach …
Early Life Experiences As Later Life Predictors For Criminality: Examination Of Public Criminal Records, Juliana S. Bastien
Early Life Experiences As Later Life Predictors For Criminality: Examination Of Public Criminal Records, Juliana S. Bastien
College of Arts & Sciences Senior Honors Theses
Crime's detrimental impact on society, victims, families, and the justice system is undeniable. Thus, identifying factors that contribute to the perpetration of crime is essential to reduce the public health impact of crime. Adverse Childhood Experiences (ACEs) are frequently found in community samples and are consistently associated with physical, psychological, and behavioral consequences. Research suggests that negative childhood experiences contribute to maladaptive behaviors, including delinquency and perpetration of crime later in life (Perez et al., 2018). The present study's purpose is to identify specific childhood experiences associated with criminality. The data presented on criminality and ACEs was collected from recruited …
Analysis And Application Of The Offense-Defense Theory: Russia, Ukraine, And History., Kirby Ballard
Analysis And Application Of The Offense-Defense Theory: Russia, Ukraine, And History., Kirby Ballard
College of Arts & Sciences Senior Honors Theses
Political scientists and government advisors have long sought to understand what influences conflicts and how to predict them. Despite constant war, a commonly used empirical theory that can answer this question has not emerged. The majority of theories created are either conflict-specific or not empirically testable. Considering these factors, I sought out a theory that would help me better understand Russia's choice to invade Ukraine in the spring of 2022. I selected the offense-defense theory due to its many attempts to explain territorial conquest, the likeliness of conflict, and overall losses. The main focus of the theory is to explain …
Impact Of Sodium Benzoate And A High Fat Diet On Kidney Of Mice., Anna Lipinski
Impact Of Sodium Benzoate And A High Fat Diet On Kidney Of Mice., Anna Lipinski
College of Arts & Sciences Senior Honors Theses
Sodium Benzoate (SB) is a commonly utilized food preservative with potential therapeutic uses for neurological conditions such as Alzheimer’s disease and hepatic encephalopathy. Consumption of SB is generally regarded as safe (GRAS), by the Food and Drug Administration, at amounts of 0.1% by weight of food. However, previous reports indicate that SB exposure may induce histological alterations in kidney structures and promote oxidative stress. Thus, with increasing obesity rates and fatty diets, the effects of SB coupled to a high fat diet (HFD) remain to be determined. This study addressed the hypothesis that SB with a HFD will increase extracellular …
Investigating The Mechanisms Through Which Autophagy Controls Lipid Levels In Cyst Stem Cells., Jared Langdon
Investigating The Mechanisms Through Which Autophagy Controls Lipid Levels In Cyst Stem Cells., Jared Langdon
College of Arts & Sciences Senior Honors Theses
With an increase in the average age of the American population, incidents of age-related diseases and illnesses are rising across healthcare communities. Addressing these pathologies with a focus towards novel treatments warrants further research into their mechanistic origins. Lipid build up within cells has been correlated with increased risk for certain pathologies, namely Alzheimer's disease and type-2 diabetes (Demarco, 2020). In this study I aimed to investigate the origin of the increase in lipids, specifically we consider the effects of lipid build up on the maintenance of stem cell populations.
Examining The Relations Between Obsessive Compulsive Disorder And Quality Of Life In A Transdiagnostic Eating Disorder Sample., Madison Rose Ortman
Examining The Relations Between Obsessive Compulsive Disorder And Quality Of Life In A Transdiagnostic Eating Disorder Sample., Madison Rose Ortman
College of Arts & Sciences Senior Honors Theses
Background: Eating disorders (EDs) are life threatening psychological disorders characterized by obsessions related to food, weight, and exercise. The presence of an eating disorders has been linked to poorer quality of life (QOL) and clinical impairment. Obsessive compulsive disorder (OCD) is a psychiatric disorder characterized by intrusive thoughts and ritualistic behaviors. EDs and OCD have similar symptom structure as well as similar independent relationships to QOL. The present study aimed to validate these relationships and extend the findings into a more fine-grained symptom analysis of the relationship between EDs, OCD and QOL. Method: Participants (N = 109) were those …
Impact Of Lyophilization On Porcine Hemoglobin Properties., Mustafa Almosawi
Impact Of Lyophilization On Porcine Hemoglobin Properties., Mustafa Almosawi
College of Arts & Sciences Senior Honors Theses
Blood transfusion is the single most often performed lifesaving procedure in hospitals worldwide. Unfortunately, packed red blood cells (RBCs) used for transfusion can only be stored for 42 days at 4 °C before being discarded due to irreversible damage that occurs during storage. Any reduction in available RBCs for an extended period can lead to blood shortages. To increase the shelf-life of RBCs, we investigated freeze-drying (lyophilizing) in the presence of the non-toxic sugar trehalose as a method for long-term preservation. However, the oxidative stress of the lyophilization and storage processes can compromise the functionality of these cells, and the …
Understanding The Role Of Emotion Regulation Tendencies In The Momentary Associations Between Negative Affect And Eating Disorder Behaviors., Coby D. Banet
College of Arts & Sciences Senior Honors Theses
Background: Eating disorders (EDs) are becoming increasingly commonplace and are associated with a broad array of dangerous medical complications. Further, EDs frequently present alongside comorbid psychiatric disorders (e.g., depressive and anxiety-related disorders), which are known to both predict ED severity and reduce treatment efficacy. While diagnostic categorization persists, Fairburn et al.’s (1993) transdiagnostic model suggests EDs may be more closely related and maintained through core, shared symptoms. To assess ED maintenance, momentary triggers such as negative affect (NA) are becoming increasingly centralized. The present study examines the roles of depressive symptoms and worry in predicting and moderating the relationships among …
The Effects Of Prescribed Fire On Ant Community Composition In A Temperate Deciduous Forest., Emma Jones
The Effects Of Prescribed Fire On Ant Community Composition In A Temperate Deciduous Forest., Emma Jones
College of Arts & Sciences Senior Honors Theses
Prescribed fire is a tool commonly used in land management to decrease wildfire frequency and promote plant diversity. However, the effects of prescribed fire on invertebrate communities, especially those within temperate deciduous forest, are poorly understood. I measured the response of epigeic ant communities in mixed mesophytic forest in Berea, Kentucky following prescribed burning. I used pitfall traps to repeatedly sample epigeic ants in replicate burned and unburned plots for up to 21 months postburn following two separate (2021 and 2022) prescribed fires. Ant species richness was similar between treatments (burn vs. control) and by burn year. Ant community composition …
An Emergentist Critique Of The Contract Theory Of The State Of Nature, With A Consideration On Two Types Of Polity And Their Origins., Ryan A. Apperson
An Emergentist Critique Of The Contract Theory Of The State Of Nature, With A Consideration On Two Types Of Polity And Their Origins., Ryan A. Apperson
College of Arts & Sciences Senior Honors Theses
The theories of the state of nature provided by the political philosophers Thomas Hobbes and John Locke have made a significant impact in the general conceptions of the origin of states. Though there are many critical differences in the conceptions of the state of nature between each in their seminal works, they both possess of a view of states that is rational and constructivist.
In this paper, I use the game theory concepts of the coordination game, collective action problem, and focal point to illustrate a lacuna in this rational and constructivist conception of the origin of states, as their …
Immobilization And Its Effects On Functional Recovery After Spinal Cord Injury., William H. Gregory
Immobilization And Its Effects On Functional Recovery After Spinal Cord Injury., William H. Gregory
College of Arts & Sciences Senior Honors Theses
Recovery, or rather the failure to recover, is a perplexing issue of spinal cord injury that is currently being investigated. This issue becomes more puzzling when investigating models in which spinal cord injury (SCI) is studied. There appears to be greater functional recovery experienced by animals within these models after injury as compared to patients in the clinical setting. While many things could contribute to this disparity, one difference that stands out when comparing the basic research model and clinical experience of SCI is the inactivity and limb disuse after the injury. This same level of immobility is rarely accounted …
The Loneliest Galaxies In The Universe: A Gama And Galaxy Zoo Study On Void Galaxy Morphology., Lori E. Porter
The Loneliest Galaxies In The Universe: A Gama And Galaxy Zoo Study On Void Galaxy Morphology., Lori E. Porter
College of Arts & Sciences Senior Honors Theses
The large-scale structure (LSS) of the Universe is comprised of galaxy filaments, tendrils, and voids. The majority of the Universe’s volume is taken up by these voids, which exist as underdense, but not empty, regions. The galaxies found inside voids are void galaxies and expected to be some of the most isolated objects in the Universe. However, their standard morphology remains poorly studied. This study, using the Galaxy and Mass Assembly (GAMA) data and Galaxy Zoo survey, aims to remedy this. For completeness purposes, we use void galaxies identified by Alpaslan et al. (2014) with stellar masses (M*) of 10 …
Histological Evaluation Of Offspring Kidneys Following Prenatal Vaping Exposure., Lucas Georges
Histological Evaluation Of Offspring Kidneys Following Prenatal Vaping Exposure., Lucas Georges
College of Arts & Sciences Senior Honors Theses
A variety of nicotine-containing products have been making their way to the market as alternatives to cigarette smoking. These include, but are not limited to, cigars, pipes, smokeless tobacco, and electronic cigarettes, with the last one being more prevalent than the others. With these alternatives, there are more tobacco users now than ever, with an increase from 1990 to 2017, which contrasts with the reduction in number of smokers observed by the Surgeon General in his Smoking Cessation report between 1990 and 2017, meaning that more and more tobacco users are using these. Pregnant women are advised to switch from …
Diversification And Convergence Following The Transition From Saltwater To Freshwater In Stingrays., Autumn D. Magnuson
Diversification And Convergence Following The Transition From Saltwater To Freshwater In Stingrays., Autumn D. Magnuson
College of Arts & Sciences Senior Honors Theses
One of the most fundamental questions in biology is why some groups of organisms are more diverse than others. Classic hypotheses for explaining differences in diversity consider distinctions in time, place, resources, and competitors as the staging grounds for differential diversification. Freshwater and saltwater environments have similar levels of diversity despite significant differences in size, so studying transitions between the two systems can provide insights into evolutionary processes. Despite the challenges associated with this transition, stingrays have invaded freshwater habitats multiple times across different continents, making them useful for better understanding these systems. In this study, I evaluated the frequency …
The Lost Cause And The Commonwealth: The United Daughters Of The Confederacy And Forging Civil War Memory In Kentucky., Emma Donaghy
The Lost Cause And The Commonwealth: The United Daughters Of The Confederacy And Forging Civil War Memory In Kentucky., Emma Donaghy
College of Arts & Sciences Senior Honors Theses
For over a century, the Kentucky division of the United Daughters of the Confederacy has worked to instill the Lost Cause myth of the Confederacy in the state’s public schools, libraries, and places where a white child could learn about the past. Few scholars have studied the activities of the Kentucky division of the UDC, although some of the organization’s most influential work took place in the state, and the organization’s national founder, Caroline Meriwether Goodlett, was born in Todd County, Kentucky. This honors thesis offers an in-depth examination of the work of the Kentucky division, drawing from the rich …
The Philosophy Of Activism And Its Paradox., Omar Arar
The Philosophy Of Activism And Its Paradox., Omar Arar
College of Arts & Sciences Senior Honors Theses
Activist organizations have been at the forefront of countless progressive efforts, seeking to ameliorate social injustices, expand the rights of marginalized people, and strengthen democratic institutions. However, the efforts of activists always seem to lead to incremental victories or a minimal change to the status quo. In this paper, I argue that the primary cause of this largely stagnant social justice landscape is the professionalization of activism. Activism in its professional form, as people who make a living out of their activist efforts, brings with it numerous issues, the most problematic among them is the manifestation a paradox. Namely, professional …
Other Bodies: Deconstructing Visual Binaries By Subverting Visual Representations Of The Other., Amaiya L. Crawford
Other Bodies: Deconstructing Visual Binaries By Subverting Visual Representations Of The Other., Amaiya L. Crawford
College of Arts & Sciences Senior Honors Theses
The goal of creating my sculptural work, The Avian, is both to broaden the visual categorization of sentient bodies, as well as to deconstruct binary thinking regarding the way that bodies display racialized, gendered, and sexualized characteristics. The Avian does this by subverting visual and aesthetic tropes that construct labels like human, monster, and animal. Subversion acts as the main design influence that illustrates how the display how othered bodies can be used to highlight the constructed nature of how we construct humanity. This paper posits that by acknowledging an expansion in visual categorization, in addition to further pushing opportunities …
The Emerging Oral Pathogen, Filifactor Alocis, Modulates Antimicrobial Responses In Primed Human Neutrophils., Ian J. Snider
The Emerging Oral Pathogen, Filifactor Alocis, Modulates Antimicrobial Responses In Primed Human Neutrophils., Ian J. Snider
College of Arts & Sciences Senior Honors Theses
Periodontitis is an irreversible, chronic inflammatory, infectious disease of the oral cavity that affects approximately half of all adults 30 years or older in the USA. The oral cavity is under high immune surveillance because of its constant exposure to microbes in the environment. The primary immune cell responsible for this surveillance is the neutrophil. Pathogens associated with periodontitis possess virulence factors and have evolved strategies to evade neutrophil antimicrobial responses to survive. One such pathogen is Filifactor alocis, whose presence is associated with the progression of periodontitis. F. alocis manipulates several neutrophil antimicrobial functional responses to avoid killing, an …
Osteoimmunology And The Reconstruction Of Host Immunological Status In Treponemal Infection: Effect Of Activated Immune Cells By Oral Pathogens And Treponema Pallidum On Osteoclastogenesis., Michayla Gatsos
College of Arts & Sciences Senior Honors Theses
Syphilitic infection caused by bacterium Treponema pallidum pallidum (Tp) offers an excellent model to study the long-lasting interplay between the immune and skeletal systems and could be used to reconstruct host immunological status. We propose that a hyper-inflammatory phenotype developed during acquired syphilis will have a systemic impact on most bone microenvironments involving inflammatory processes, such as the one developed in periodontal disease (PD). Using osteoimmunological in vitro protocols, we explore whether immune activation by Tp antigens and PD pathogens can impact osteoclastogenesis (OCG), ultimately helping to understand the bone alterations caused by systemic inflammatory processes in skeletal material. We …
Azanyl Carbamates: Synthesis, Characterization And Chemistry., Maunil Mullick
Azanyl Carbamates: Synthesis, Characterization And Chemistry., Maunil Mullick
College of Arts & Sciences Senior Honors Theses
Azanyl carbamates are an understudied, rarely-reported class of compounds in the organic chemistry literature. The azanyl carbamate moiety contains both a carbamate and an amine functional group. As such, this class of compounds features a high ratio of heteroatoms (atoms that are not carbon or hydrogen) relative to carbon: a characteristic of many natural and synthetic organic catalysts.1 Another reason we are particularly interested in azanyl carbamates is the fact that the amine is incorporated as an aminooxy group. An aminooxy group consists of an amine directly connected to an oxygen atom. The aminooxy group is of special interest …
Impacts Of Dietary Restriction On A Drosophila Model Of Werner Syndrome, Eileen Sember
Impacts Of Dietary Restriction On A Drosophila Model Of Werner Syndrome, Eileen Sember
College of Arts & Sciences Senior Honors Theses
Werner syndrome (WS) is an autosomal recessive disorder that results in premature aging and occurs in 1 in 1,000,0000 to 1 in 10,000,000 people. In humans, WS is the result of mutations that render the WRN gene, that contains a helicase and an exonuclease domain, non-functional. Currently, there is no cure for WS in humans, making dietary and lifestyle interventions attractive for increasing the quality and longevity of lives. Diet restriction (DR) has been shown to extend the lifespan of several model organisms, including Drosophila melanogaster, making it a strong candidate for WS treatment. In this thesis, mutant flies …
Impacts Of Bacterial Evolution On Host Lethality In Drosophila, Andrew Preston
Impacts Of Bacterial Evolution On Host Lethality In Drosophila, Andrew Preston
College of Arts & Sciences Senior Honors Theses
Evolution is the process by which species change their genetic traits, such as the pathogenicity of bacteria, over time in response to changes in their environment. Although the genetic mechanisms underlying many evolutionary processes have been revealed, it is still not well understood how opportunistic pathogens, such as Pseudomonas aeruginosa, become virulent. The overall goal of this thesis is to test the Coincidental Evolution Hypothesis, which proposes that the virulence of opportunistic pathogens evolves coincidentally as a by-product of their interaction with their natural predators. I hypothesized that the virulence of ancestral Pseudomonas aeruginosa changes over time if it co-evolves …
Understanding The Evolution Of Enforcement Systems In Resource Dilemmas, Devin M. Flener
Understanding The Evolution Of Enforcement Systems In Resource Dilemmas, Devin M. Flener
College of Arts & Sciences Senior Honors Theses
The effective management of resources on Earth is a pressing global social dilemma. An alternative solution to the traditional managing methods of these common pool resources is communal self-management facilitated through principles of restorative justice and developed through exploratory learning. We examined how communication, restorative justice techniques, and opportunity for exploratory learning impacted groups’ ability to develop a strong conceptual understanding of enforcement as well as an enforcement system in order to maintain an effective conservation strategy. Participants (N=288) were randomly assigned to 72 four-person groups in six separate conditions. Each group played nine rounds of a computer-simulated foraging task …
Interpreting San Cecilio: Ritual And Discourse In A Granadan Celebration., Martha M. Popescu
Interpreting San Cecilio: Ritual And Discourse In A Granadan Celebration., Martha M. Popescu
College of Arts & Sciences Senior Honors Theses
The romería de San Cecilio is an annual, local short pilgrimage and celebration of the patron saint of Granada, a city in Andalusia, Spain. The romería takes place at the Abbey of Sacromonte, a monastery built on top of the site where San Cecilio’s remains were found as part of the famous discoveries of the Lead Books of Granada in the late sixteenth century. These books were ultimately declared to be Islamic forgeries, yet the romería persists today as a granadino, or Granadan, tradition. Consisting of both a Mass at the Abbey as well as a popular celebration, the …