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Jettability Of Phthalocyanine Dye Ink Formulations, Yumeizhi Jia Apr 2013

Jettability Of Phthalocyanine Dye Ink Formulations, Yumeizhi Jia

Research and Creative Activities Poster Day

No abstract provided.


Hybrid Rapid Prototyping Approach For Rapid Casting Applications, Hemant Bohra Apr 2013

Hybrid Rapid Prototyping Approach For Rapid Casting Applications, Hemant Bohra

Research and Creative Activities Poster Day

No abstract provided.


Multiphoton Fluorescence Spectroscopy To Monitor The Unfolding Of The Green Fluorescent Protein, Ibtesam Y. Alja'afreh, Ramakrishna Guda, David Huffman Apr 2013

Multiphoton Fluorescence Spectroscopy To Monitor The Unfolding Of The Green Fluorescent Protein, Ibtesam Y. Alja'afreh, Ramakrishna Guda, David Huffman

Research and Creative Activities Poster Day

Green fluorescent protein (GFP), from Aequorea coerulescens, has been expressed as a His-tag fusion protein in E. coli cells and purified using metal chelate affinity and gel filtration chromatography. The protein fluoresces at 510 nm due to its natural chromophore that is spontaneously formed after properly folding and remains stable and intact after protein unfolding. The chromophore is buried in a beta barrel and has a permanent dipole moment in both the ground state and the excited state. The difference between permanent dipole moments Δμge is highly proportional to the electrostatic environment around the chromophore. Since Δμge …