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Structural Basis Of Epigenetic Regulation And Protein Scaffolding In Development And Diseases, Yuanyuan Jiang

Wayne State University Dissertations

Protein X-ray crystallography is a powerful approach for elucidating protein structure and function. The high-resolution data generated by X-ray allow us to visualize protein structures in a three-dimensional (3D) space, which is vital for our understanding of the protein intra- and intermolecular interactions that explain the mechanisms of various biological events. More importantly, such information can provide a structural basis for developing new methods and strategies of targeted drug discovery. In this dissertation, by using X-ray crystallography as the primary approach, we have performed the structural and functional studies of SMYD2 and NHERF1 and have determined their mechanisms of action …

General Transcription Factors Play Dual Roles In Initiation And Termination, Scott Alan Medler

Wayne State University Dissertations

Gene looping, defined as the interaction of the promoter and the terminator regions of a gene during transcription, is emerging as an important gene regulatory mechanism in eukaryotes. The role of promoter bound general transcription factors during initiation is well established. However, recent studies have revealed that some initiation factors also interact with the 3' end of a gene. The biological role of initiation factors at the 3' end of a gene is unknown. The general transcription factors TFIIB and TFIIH have been found to interact genetically with Ssu72, a component of CPF 3' end processing complex. Accordingly, we found …

An Analytics Approach To Designing Patient Centered Medical Home, Saeede Ajorlou

Wayne State University Dissertations

Recently the patient centered medical home (PCMH) model has become a popular team based approach focused on delivering more streamlined care to patients. In current practices of medical homes, a clinical based prediction frame is recommended because it can help match the portfolio capacity of PCMH teams with the actual load generated by a set of patients. Without such balances in clinical supply and demand, issues such as excessive under and over utilization of physicians, long waiting time for receiving the appropriate treatment, and non continuity of care will eliminate many advantages of the medical home strategy. In this research, …

An Analytics Approach To Reducing Hospital Readmission, Issac Shams

Wayne State University Dissertations

One of the significant sources of waste in the Unites States health care systems is preventable hospital readmission. About 2.3 million Medicare fee-for-service beneficiaries are re-hospitalized within 30 days after discharge which incurs an annual cost of $17 billion. However, it is reported by the Medicare Payment Advisory Commission that about 75% of such readmissions can and should be avoided because they are the results of factors such as poor planning for follow up care transitions, inadequate communication of discharge instructions, and failure to reconcile and coordinate medications. Hence, reducing unnecessary rehospitalization through care transition and systems engineering principles has …

Trauma Exposure, Posttraumatic Stress, And Depression In A Community Sample Of First-Time Mothers, Mickey Sperlich

Wayne State University Dissertations

The adverse effects of posttraumatic stress and depression have separately been well-documented in the perinatal mental health literature. However, few studies have considered the comorbidity between trauma, posttraumatic stress and depression. This dissertation study brings attention to this comorbidity and explores implications of recent changes to diagnostic criteria for posttraumatic stress disorder related to the ability to predict postpartum depression and impairments in mother/infant bonding. Following a conceptual framework which outlines the effects of violence and trauma on adverse childbearing outcomes, hypotheses were that many women with depression in pregnancy would endorse trauma and would be at risk for subthreshold …

Muscle Metaboreflex Control Of Cardiovascular Function In Hypertension, Marty Daniel Spranger

Wayne State University Dissertations

Skeletal muscle ischemia during or immediately following exercise leads to the accumulation of metabolites (e.g., lactate, proton and diprotonated phosphate) which activate chemoreceptive afferents within the muscle leading to a reflex increase in sympathetic outflow generating substantial increases in mean arterial pressure (MAP), cardiac output (CO) and heart rate (HR) - termed the muscle metaboreflex. When the reflex is activated during submaximal dynamic exercise, the pressor response occurs via increased CO with no net peripheral vasoconstriction. When metaboreflex activation is sustained during the recovery from exercise (i.e., post-exercise muscle ischemia - (PEMI)), whereas MAP remains elevated for as long as …

Jp-8 Surrogates For Diesel Engine Application: Development, Validation, And Cfd Simulation, Amit Shrestha

Wayne State University Dissertations

Recently, diesel engine cycle simulation has been acknowledged as an effective way for the development and performance optimization of compression ignition engines operating on a variety of fuels. The goal of this research was, therefore, to develop a JP-8 surrogate with a limited number of fuel components so that its chemical mechanism could be developed for its use with computational fluid dynamics (CFD) codes for enabling time-efficient diesel engine cycle simulation. In order to achieve this goal, a new approach was developed for the formulation of surrogate for compression ignition engine application. The development approach required the surrogate to match …

Influence Of Siop Cognitive Coaching Workshops On Teaching Practices Of Esl Teachers And Esl Paraprofessionals, Ruben Alicea

Wayne State University Dissertations

The purpose of this study was to explore and report on the influence that cognitive coaching, as an embedded part of professional development, has on ESL teachers' and ESL paraprofessionals' learning and practice in the context of educating English learner (EL) students using the Sheltered Instruction Observation Protocol (SIOP) model of instruction. An examination was made on the views of 52 ESL teachers and paraprofessionals regarding participation in a series of 12 professional development workshops, use of cognitive coaching, and implementation of the SIOP with their EL students. As part of their participation in the professional development, self-reflection data were …

Using Large-Angle Beamstrahlung To Detect And Diagnose Colliding Beams For Luminosity Optimization, Hussein Farhat

Wayne State University Dissertations

The large-angle-beamstrahlung is a very effective technique that can be used to monitor two crossing beams. Monitoring the beam helps in analyzing and studying the beams profiles, allowing beams adjustments, and eventually leading to a perfect beams collision. The perfect beam collision results in a higher luminosity; as a result, a high particles production rate and new physics can be discovered.

A Daily Study Of The Sleep-Pain Relationship In Fibromyalgia, Maren Elizabeth Hyde-Nolan

Wayne State University Dissertations

Fibromyalgia (FM) impacts millions of individuals around the world and is characterized by widespread chronic pain and tenderness as well as nonrestorative sleep, fatigue, and stiffness (Wolfe et al., 1990; Wolfe et al., 2010). Poor sleep quality is reported by more than 90% of individuals with FM, suggesting that sleep disturbance may be a contributing factor to the pain experience (Moldofsky, 2008). Recent reviews of the literature have established the connection between sleep and pain, although the direction of this relationship remains unclear (Finan et al., 2013; Moldofsky, 2001). This dissertation sought to examine the daily relationship between sleep and …

Algorithms And Tools For Computational Analysis Of Human Transcriptome Using Rna-Seq, Nan Deng

Wayne State University Dissertations

Alternative splicing plays a key role in regulating gene expression, and more than 90% of human genes are alternatively spliced through different types of alternative splicing. Dysregulated alternative splicing events have been linked to a number of human diseases. Recently, high-throughput RNA-Seq technologies have provided unprecedented opportunities to better characterize and understand transcriptomes, in particular useful for the detection of splicing variants between healthy and diseased human transcriptomes.

We have developed two novel algorithms and tools and a computational workflow to interrogate human transcriptomes between healthy and diseased conditions. The first is a read count-based Expectation-Maximization (EM) algorithm and tool, …

Development Of Elisa-Based Hdac Activity Assay For Identification Of Isoform Selective Hdac Inhibitors, Geetha Padige

Wayne State University Dissertations

Cancers are one of the leading causes of death in United States, effecting roughly 20% of the population. To develop effective anti-cancer agents, vast research is in progress that targets various factors leading to cancer. One such area of cancer research is developing drugs that inhibit histone deacetylase (HDAC) proteins. HDACs are histone modifying enzymes that regulate transcription of genes. Aberrant recruitment of HDACs to the transcription factors leads to tumor onset and growth. Because of their potential anti-cancer therapeutic interest, several HDAC inhibitors are in various stages of clinical trials. SAHA and Romidepsin are FDA-approved drugs for the treatment …

Building A Scalable And High-Performance Key-Value Store System, Yuehai Xu

Wayne State University Dissertations

Contemporary web sites can store and process very large amounts of data. To provide timely service to their users, they have adopted key-value (KV) stores, which is a simple but effective caching infrastructure atop the conventional databases that store these data, to boost performance. Examples are Facebook, Twitter and Amazon. As yet little is known about the realistic workloads outside of the companies that operate them, this dissertation work provides a detailed workload study on Facebook's Memcached, which is one of the world's largest KV deployment. We analyze the Memcached workload from the perspective of server-side performance, request composition, caching …

In Particularity We Trust:Richard Dutcher's Mormon Quartet And A Latter-Day Saint Spiritual Film Style, Mark Sheffield Brown

Wayne State University Dissertations

Between 2000 and 2008, writer/director Richard Dutcher made four films with narratives focused primarily on members of the Church of Jesus Christ of Latter-Day Saints. The films are explicitly Mormon-related in their content, but I argue they are also inherently Mormon in their style. Critic and filmmaker Paul Schrader argues there is a particular style of filmmaking, a dialect of the cinematic language if you will, that enables viewers to experience an encounter with a Transcendent Divinity. The contention of this dissertation is that Schrader's views were simultaneously too general and too narrow. I draw on Clive Marsh's call for …

Correlation Effects In Nanoparticle Composites: Percolation, Packing And Tunneling, Rupam Mukherjee

Wayne State University Dissertations

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Longitudinal Change In Regional Cortices And Fluid Intelligence, Peng Yuan

Wayne State University Dissertations

Fluid intelligence (Gf) and crystalized intelligence (Gc) are two factors of the general intelligence. They have distinct age-related trajectories of change. Jung and Haier proposed Parieto-Frontal Integration Theory (P-FIT, 2007) to account for the inter-person variance in reasoning intelligence. Some brain regions such as prefrontal, parietal, temporal and anterior cingulate cortices were included in the P-FIT model and were hypothesized to be involved in fluid reasoning task. Therefore, in the current study, we examined latent growth curves (LGC) of longitudinal change in Gf, Gc, prefrontal cortex, parietal cortex, anterior cingulate, temporal cortex and primary visual cortex. Forty-six healthy middle-aged and …

Subjugated Bodies, Normalized Subjects: Representations Of Power In The Panamanian Literature Of Roberto Díaz Herrera, Rose Marie Tapia And Mauro Zúñiga Araúz, Sara Escobar-Wiercinski

Wayne State University Dissertations

This dissertation examines the dissemination of power represented in the works of Panamanian writers Roberto Díaz Herrera, Rose Marie Tapia and Mauro Zúñiga Araúz. My work focuses on two important periods in Panama's history: the repressive dictatorial era of Manuel Noriega and the post-dictatorial era during which subjugation and power operate in subtle ways, through institutions, mechanisms of civil society, and globalization. The primary sources are Díaz Herrera's testimony, and the novels of Tapia and Zúñiga Araúz. In my analysis, I draw upon the notions of power, subjugation and normalization developed by the French philosoher Michel Foucault. I also draw …

Biofuels Production Via Catalytic Hydrocracking Of Ddgs Corn Oil And Hydrothermal Decarboxylation Of Oleic Acid Over Transition Metal Carbides Supported On Al-Sba-15, Basem A. Al Alwan

Wayne State University Dissertations

The purpose of this research is to minimize the gap between the production of biofuels and the production of petroleum-based fuels by developing catalysts that can utilize renewable non-food based feedstocks (waste vegetable oils, algal oil, brown grease, etc.) and have great performance under low operation conditions. In particular, green diesel has become an attractive biofuel due to its superior properties that are quite similar to petroleum diesel. Therefore, no modifications are required to existing infrastructures. Three distinct experimental phases have been identified in order to achieve the objective of this work as follow:

First, the hydrocracking of distillers dried …

Environmental Stressors: Pathways Of Exposure And Aquatic Invertebrate Response, Carly Jean Nowicki

Wayne State University Dissertations

The need to monitor freshwater and detect impairments prior to observable impacts is crucial to maintain species diversity and ecosystem function. Therefore, understanding the contribution of various matrices (i.e., sediment and water) to chemical exposure is critical for remediation of impacted sites.

To evaluate various matrices of chemical exposure, I focused on the organic pollutant polychlorinated biphenyls (PCBs) due to their relatively ubiquitous nature, high toxicity, and adverse effects on humans and wildlife. In complementary laboratory and field experiments, I compared the effects of aqueous versus sedimentary exposure of PCBs on invertebrates. In the laboratory, organisms exposed to PCB-contaminated sediments …

Lanthanide-Based Precatalysts For Carbon-Carbon Bond-Forming Reactions In Aqueous Media, Derek James Averill

Wayne State University Dissertations

The formation of carbon-carbon bonds is of great interest to synthetic chemists because these bonds make up the majority of biologically active compounds. The Mukaiyama aldol reaction is a Lewis-acid-catalyzed carbon-carbon bond-forming reaction that has the ability to produce optically active β-hydroxy carbonyls which can be found in many pharmaceuticals and natural products. Because of precatalyst instability towards hydrolysis, anhydrous solvents are commonly used. Recent efforts have focused on water-tolerant versions of enantioselective Mukaiyama aldol reactions because of the financial and environmental benefits of using aqueous media. Consequently, the Lewis-acidic and water-tolerant features of Ln³⁺ ions have aroused great …

Theoretical And Experimental Studies Of Noncovalent Metal Cation Binding And Base Pairing Interactions Of Cytosine And Modified Cytosines, Bo Yang

Wayne State University Dissertations

ABSTRACT

THEORETICAL AND EXPERIMENTAL STUDIES OF NONCOVALENT METAL CATION BINDING AND BASE PARING INTERACTIONS OF CYTOSINE AND MODIFIED CYTOSINES

by

BO YANG

December 2014

Advisor: Professor Mary T. Rodgers

Major: Analytical Chemistry

Degree: Doctor of Philosophy

Binding of metal cations to the nucleobases can lead to formation of rare tautomers of the nucleobases. The infrared multiple photon dissociation (IRMPD) action spectroscopy of five alkali metal cation-cytosine complexes, M⁺(cytosine), where M⁺ = Li⁺, Na⁺, K⁺, Rb⁺, and Cs⁺, are examined using a Fourier transform ion cyclotron resonance mass …

Mental Health Service Utilization Among African-American Adolescents, Marilyn Franklin

Wayne State University Dissertations

Underutilization of mental health services by ethnic minorities has been identified as a major public health threat by the U.S. Department of Health and Human Services (2001). Research on ethnic disparities has focused on identifying the sociodemographic correlates of between group differences in treatment attendance. The current study adds to the utilization literature by exploring within group differences amongst a sample of African-American families. Based on extant literature related to treatment utilization in other samples, this study examined the associations between multiple modifiable factors and adolescent treatment engagement amongst 90 African-American caregiver-adolescent dyads.

Consistent with previous research, psychological symptoms, functional …

Factors Related To Quality Of Life In Families Of Children With Autism Spectrum Disorder, Jessica R. Garrett

Wayne State University Dissertations

The purpose of this study was to explore how caretakers of children diagnosed with an autism spectrum disorder (ASD) are able to move through adverse circumstances with which they are confronted while raising their child with considerable developmental needs and challenges. Family resilience in this study includes family quality of life, locus of control, sense of coherence, perceived stress, uncertainty, severity, and demands. The participants in this study were 153 parents of children diagnosed with ASD. The parents were members of the Autism Society of Oakland County. The participants completed a survey comprised of six scales (Parental Concerns Questionnaire, Perceived …

Health-Related Behaviors In Low-Income, Minority Youth: The Role Of Motivation, Basic Needs, Mental Health, And Environmental Barriers, Brittany A. Kohlberger

Wayne State University Dissertations

Engaging in health-related behaviors, such as exercise, healthy eating, and sleep hygiene, are associated with fewer externalizing and internalizing behavior problems and higher self-esteem, suggesting that there may be large benefits to engaging in health-related behaviors during the transitional developmental phase of adolescence (Ong, Wickramaratne, Tang, & Weissmann, 2006; Stathopoulou, Power, Berry, Smits, & Otto, 2006). The purpose of the current study was to identify key psychological processes and external factors that may boost or impede youths' motivation and engagement in health-related behaviors. Specifically, we expected that higher levels of psychological symptoms and environmental barriers will be significantly, negatively associated …

A Cross-Sectional Study Of The Relations Between Parental Factors And Academic Achievement In High School Adolescents, Patricia Marie Lanzon

Wayne State University Dissertations

Bronfenbrenner's (1979) ecological model was used as the theoretical model for this study of parental achievement factors that influence adolescent academic achievement. Many predictors are related to a child's potential for academic achievement. Of the most proximal are parental and family factors, which were the focus in this study. The proximal parental and family factors for this study were as follows: family structure, maternal and paternal parenting styles and parental involvement, parents' school and community involvement, parental attitude toward achievement, and free and reduced lunch status. Achievement as determined by the student's actual grade point average was the dependent variable. …

Study Of Tin-Based Electrodes And Ionic Liquid Electrolytes For Energy Storage Materials, Mahbuba Ara

Wayne State University Dissertations

Due to rapid increase in energy demand, modern society necessitates to develop high power, light-weight, and more economical energy storage systems. Rechargeable Li-ion batteries and Li-oxygen batteries have become the most promising energy devices in terms of energy and power densities. Diverse research on these battery components is being carried out by researchers worldwide to improve power density to meet the future requirements. The possible routes to improving power density of Li-ion as well as Li-oxygen batteries is to use nanostructured, hybrid electrode materials since they can significantly enhance kinetics of electrochemical reactions; and ion-conducting, low volatile electrolytes since they …

Coupled Sediment Yield And Sediment Transport Model To Support Navigation Planning In Northeast Brazil, Calvin Trebor Creech

Wayne State University Dissertations

Deposition of sediment (shoaling) in commercial waterways is a major obstacle to maintaining sustainable riverine transportation of bulk goods (primarily agricultural and mining commodities). The rate of aggradation of sediment in a waterway is directly related to both the rate of sediment erosion from upland and river bank sources (sediment yield) and the energy in the river to effectively transport the sediment through the waterway system (sediment transport). Historically, methods used for waterway development have included trial and error or rules of thumb associated with river training structures and chute cut-off canals or engineering of navigation locks and dams. More …

Annihilation And Accumulation: Postcolonial Literatures On Genocide And Capital, Shashi Thandra

Wayne State University Dissertations

The emergence of South-South relations in politics and economics refracts strangely through the literature produced in these postcolonial regions. Two primary worldviews emerge in these texts. The first focuses on the continued presence of imperial powers in the South and their culpability in eruptions of violence. The second shifts to modes of domination emerging within South-South interactions. Salman Rushdie's canonical Midnight's Children examines the Bangladeshi genocide through a variety of literary strategies, especially hyperbole, to produce a crisis of history to indict the Cold War arms trade on equal terms with a war criminal. Similarly, Boubicar Boris Diop's novel Murambi, …

The Circle Of Courage: Childhood Socialization In The 21st Century, William Charles Jackson

Wayne State University Dissertations

The aim of this investigation was to examine the psychometric properties of the Circle of Courage Scale. Data was collected from 51 youth ages 13-17 enrolled in program at Starr Commonwealth a non-profit human services organization headquartered in Michigan. Data was collected over a period of 9 months and used as an aggregate data base. Principal components factor analysis with varimax rotation resulted in a four-factor solution as determined by eigenvalues greater than one, simple structure convergence, item loadings and conceptual clarity. The four components of the Circle of Courage were labeled as Adult/Peer Influence, Belonging, Mastery and Peer Culture. …

Using Video Games For Decreasing Pain Caused By Acute Painful Crisis In Adolescents With Sickle Cell Pain, Talal Ali

Wayne State University Dissertations

Purpose: The most common symptom of sickle cell disease is pain, which occurs as the cells clump compromising further blood flow to distal organs. Despite the advancement in pain management, many children and adolescents' pain remains under treated. The purpose of this study is measure the effectiveness of a videogame as a developmentally appropriate non-pharmacological modality on pain in adolescents 12-21 years of age with sickle cell crisis.

Methods: A one-group repeated measure quasi-experimental design was used to evaluate the effectiveness of using videogames on pain in a sample of adolescents 12-21 years of age. Using a repeated measures design …