Digital Commons Network^™

The Sweet Smell Of Mystery: The Scent Glands Of Oncopeltus Fasciatus, Despina Tsitlakidou

Wayne State University Theses

Scent glands (ScGs) are defensive glands that are found among a wide variety of insect orders. These glands represent a specialized function of the exocrine system; however, the developmental mechanism remains unclear. Previous functional studies of the endocrine glands revealed that the endocrine and tracheal systems utilize similar genetic regulatory networks which indicate that these systems have common primordia. In Drosophila, sal is localized in the prothoracic gland while in Oncopeltus the gene is localized in the duct cells of the abdominal scent glands (Hanna & Popadić, 2020; Sánchez-Higueras et al., 2014). RNAi mediated knockdown of sal resulted in significant …

Spatial Localization Of Markers And 3d-Cell Model For Study Of The Human Cytomegalovirus Cytoplasmic Virion Assembly Compartment, Taylor Alan Vensko

Wayne State University Theses

Human cytomegalovirus (HCMV) causes severe disease in immunocompromised individuals and is a leading cause of congenital disease. Efficient assembly of virions (viral particles) is an intricate process that requires modulation and modification of host systems. HCMV induces an extensive rearrangement of the cellular endocytic recycling compartment into the site of virion maturation and egress: the cytoplasmic virion assembly compartment (cVAC). The HCMV cVAC is a distinguishing characteristic of infection, although studies have only been performed in traditional cell culture with limited observations in infectedhuman tissues. To study the cVAC, viral and cellular markers that are discrete to their respective compartments …

Reliability And Validity Of A Survey To Analyze Job-Related Stress And Self-Effifacy In Early Childhood Education Workorce Professional Identity Development, Amanda Tinelle Batts

Wayne State University Theses

The field of early childhood education is one of high stress and low compensation. Yet, little is known about how the increased demands for accountability and professionalization within the field impact the workforce. This study examines whether educators in high- and low-quality early learning settings experience differences in professional identity development, work-related stress, and professional dissonance between feelings of authority and vulnerability. Previous research on these issues has been limited to small qualitative studies that have shown that educators may experience more stress and professional dissonance in conjunction with increases in their professional experience and demands on their time to …

Computational Single-Cell Analysis Of Confocal Fluorescence Images With Dapi-Generated Masks, Munirah Alduhailan

Wayne State University Theses

Lipolysis is a metabolic pathway in which free fatty acids are mobilized from stored triglycerides. The rate-limiting enzyme in this process is adipose triglyceride lipase, which is regulated by α/β-hydrolase domain-containing protein 5 (ABHD5) via both natural and synthetic pathways. With advanced artificial neural networks, image processing methods can extract quantitative results from fluorescence images. The segmentation of complex biological images, in which regions of the image are labeled as distinct masks, is the first step in image analysis. Ilastik, a machine-learning software, performs image segmentation with a user-trained neural network and custom key feature labels. The software’s results are …

Defending Against Adversarial Attacks On Medical Imaging Ai Systems, Xin Li

Wayne State University Theses

Although deep learning systems trained on medical images have shown state-of-the-art performance in many clinical prediction tasks, recent studies demonstrate that these systems can be fooled by carefully crafted adversarial images. It has raised concerns on the practical deployment of deep learning based medical image classification systems. Although an array of defense techniques have been developed and proved to be effective in computer vision, defending against adversarial attacks on medical images remains largely an uncharted territory due to their unique challenges: crafted adversarial noises added to a highly standardized medical image can make it a hard sample for model to …

The House Detroit Built: House Music In Techno City, Keaton Jose Soto-Olson

Wayne State University Theses

Although house music was invented in Chicago, the genre has flourished in Detroit. Legendary DJs like Ken “The Godfather” Collier and Stacey “Hotwaxx” Hale, helped pave the way for generations of house DJs and producers in Detroit, a city more known for producing techno. The genres of techno and house are related but distinct, the work of Detroit producers that worked in both styles proves as a useful case study for making distinctions between the two genres. This work examines the history and current practice of Detroit house music, contrasting it with Detroit techno and Chicago house. Using scholarly literature …

Functional Characterization Of Threonine 49 Phosphorylation Of Cytochrome C, Antoine Khobeir

Wayne State University Theses

Cytochrome c (Cytc) is a pivotal multifunctional mitochondrial protein that serves as a single electron carrier between complexes III and IV of the electron transport chain. It has important roles in both cellular respiration and apoptosis. The novel Thr49 (T49) phosphorylation of Cytc likely affects mitochondrial respiration, membrane potential, ROS production, ATP production, and apoptosis. Based on the functional characterization of previously mapped phosphorylation sites (Tyr97, Tyr48, Thr28, Ser47, Thr58) of the lab, we hypothesize that T49 phosphorylation will lead to controlled respiration, optimal intermediate mitochondrial membrane potential, lower ROS production, and inhibition of apoptosis compared to unphosphorylated Cytc. Here …

The Relationships Among Personality Traits, Food Consumption, And Anthropometrics In Healthy People, Chelsea Schwartz

Wayne State University Theses

Two out of three people are obese in the United States, but Western culture isn’t the only population suffering from obesity and comorbidities. Many factors contribute to this global epidemic and more recently various research suggests that personality traits can be used to predict eating behaviors. This study investigated the Big Five personality trait’s influence on food consumption to determine what traits contribute to healthy eating and what traits are susceptible to overeating and therefore, poor health consequences. The Big Five personality traits, anthropometric measures, and diet logs were used to assess 38 individuals for health-related behaviors and obesity-related risk …

Oral Cbd Administration: Assessing Bioavailability And Behavioral Outcomes In A Rodent Model, Jordan Skully

Wayne State University Theses

There has been a recent surge in popularity for cannabidiol (CBD), a major non-psychotropic constituent of cannabis, due to numerous claims of potential therapeutic properties, which include, but are not limited to, anxiolytic, antinociceptive, and anti-inflammatory effects. However, as previous scientific literature on CBD's effectiveness in providing such therapeutic effects is limited, this project was aimed to evaluate the potential beneficial properties of a hemp derived 99% pure CBD compound provided from Ellipse Analytics (Denver, CO) in a rodent model. We analyzed the pharmacokinetics of this CBD product as well as the behavioral outcomes after acute and chronic administration. Pharmacokinetics …

Novel Design And Facile Synthesis Of Functional Nanostructures For Energy And Environmental Application, Zhuojun Wang

Wayne State University Theses

Environmental pollution and energy crisis are two of the major obstacles in the development of a more sustainable society. It is an interesting but technically challenging task to design novel methods for the facile and scalable synthesis of multifunctional materials for applications in both energy storage and environmental remediation. In this thesis, I tried to design and synthesize novel nanostructured materials for energy and environmental applications, achieved highly promising results. I developed a facile electrochemical approach to synthesize the zinc oxide with the zinc element for photocatalytic reactions. It demonstrated dramatically improved performances in degradation of organic pollutant in water, …

Novel Role Of Endoplasmic Reticulum-Associated Degradation In The Regulation Of Ceruloplasmin, Stephen William Hippleheuser

Wayne State University Theses

Ceruloplasmin (Cp) is a secreted ferroxidase produced by the hepatocytes that assists in the transport of iron throughout the human body. In human aceruloplasminemia patients, due to Cp deficiency excess intracellular iron buildup leads to ailments like liver cirrhosis, neurodegeneration, and blindness. We recently found that the biogenesis of Cp in the endoplasmic reticulum (ER) is regulated by a principal ER quality-control process, ER-associated degradation (ERAD). ERAD clears misfolded ER proteins for cytosolic proteasomal degradation, with the Sel-1 suppressor of lin-12-like (Sel1L)-HMG-CoA reductase degradation 1 (Hrd1) protein complex representing the most conserved branch in mammals. Interestingly, we found that Sel1L-Hrd1 …

Process Data Analytics Using Deep Learning Techniques, Majid Moradi Aliabadi

Wayne State University Theses

In chemical manufacturing plants, numerous types of data are accessible, which could be process operational data (historical or real-time), process design and product quality data, economic and environmental (including process safety, waste emission and health impact) data. Effective knowledge extraction from raw data has always been a very challenging task, especially the data needed for a type of study is huge. Other characteristics of process data such as noise, dynamics, and highly correlated process parameters make this more challenging.

In this study, we introduce an attention-based RNN for multi-step-ahead prediction that can have applications in model predictive control, fault diagnosis, …

Generation Of Neural Stem Cells (Nscs) From Human Fibroblasts Using Qq-Modified Sox2 And Neurod1 Proteins, Abdullah Ibrahim Alhomoudi

Wayne State University Theses

The generation of induced neural stem cells (iNSCs) and induced neuronal cells (iNCs) from somatic cells provides new avenues for basic research and potential transplantation therapies for neurological diseases. However, clinical applications must consider the tumor formation capabilities of the implanted cells, the inability of iNCs to self-renew in culture, and reprogramming methods that use retroviral transduction which permanently alter genetic network of the cells. Here we report the generation of protein-induced neural stem cells (piNSCs) from human dermal fibroblasts using QQ-SON pluripotent reprogramming as a tool to quickly reset the time clock of the human somatic fibroblasts to a …

Representation Learning With Autoencoders For Electronic Health Records, Najibesadat Sadatijafarkalaei

Wayne State University Theses

Increasing volume of Electronic Health Records (EHR) in recent years provides great opportunities for data scientists to collaborate on different aspects of healthcare research by applying advanced analytics to these EHR clinical data. A key requirement however

is obtaining meaningful insights from high dimensional, sparse and complex clinical data. Data science approaches typically address this challenge by performing feature learning in order to build more reliable and informative feature representations from clinical data followed by supervised learning. In this research, we propose a predictive modeling approach based on deep feature representations and word embedding techniques. Our method uses different deep …

Characterization, Modeling, And Thermal Management Of High-Performance Lithium Batteries, Minjun Bae

Wayne State University Theses

Lithium-ion (Li-ion) batteries, as one of the most advanced commercial rechargeable batteries, play a crucial role in modern society as they are extensively used in portable electronic devices. Nevertheless, the limited electrochemical performance and poor thermal management systems of Li-ion batteries have hindered the expansion of their future applications. In search of alternative electrode materials to develop a battery with higher electrochemical performance, lithium (Li) metal has attracted much attention as an ideal alternative anode material due to its high specific capacity and lowest redox potential. However, needle-like Li dendritic growth causes severe safety concerns and thus prohibits practical applications …

Politics At Play: The 1985 World Festival Of Youth And Students And Its Role In Soviet And Cold War History, Michaela Lewalski

Wayne State University Theses

The Twelfth World Festival of Youth and Students that took place in Moscow in 1985 has largely been forgotten, but historical analysis of the event reveals that it had significant implications for the Soviet Union and Cold War. This thesis argues that the festival was a public ceremony that the Soviet Union used to prove its domestic stability and its role as a leader in the fight for world peace to its own people, counterparts in the West, and allies and potential allies in the South and East. The symbols and concrete measures that the Soviet Union used—and the reactions …

A Field Study Of Bioaccessible Lead In Detroit Soils: Insight Into The Effectiveness Of Phosphate-Based Lead Sequestration, Sabrina R. Good

Wayne State University Theses

Historical and contemporary use of lead (Pb) in gasoline, paints, and industry have caused lead to be ubiquitous in the urban soil environment, disproportionately affecting low-income minority children. As soil is a major exposure pathway for children, an effective remediation technique for lead-contaminated soil is urgently needed. Common remediation techniques, such as excavation or soil capping, are expensive and environmentally destructive, especially on a city-wide residential basis. Decreasing the bioavailability of lead, or the fraction which is retained by the human body, may be a more economically and environmentally conscious option for remediating lead in urban environments.

Research has demonstrated …

Text Mining Of Variant-Genotype-Phenotype Associations From Biomedical Literature, Nafiseh Saberian

Wayne State University Theses

In spite of the efforts in developing and maintaining accurate variant databases, a large number of disease-associated variants are still hidden in the biomedical literature. Curation of the biomedical literature in an effort to extract this information is a challenging task due to i) the complexity of natural language processing, ii) inconsistent use of standard recommendations for variant description, and iii) the lack of clarity and consistency in describing the variant-genotype-phenotype associations in the biomedical literature. In this article, we employ text mining and word cloud analysis techniques to address these challenges. The proposed framework extracts the variant-gene-disease associations from …

Post Chemotherapeutic Treatment Impact On Pediatric Patients With Acute Lymphoblastic Leukemia, Roaa Alfarshooti

Wayne State University Theses

Acute lymphoblastic leukemia (ALL) is the most prevalent type of leukemia among children. It is characterized by chromosomal and genetic variations and abnormalities occurring in the leukemic cells. Prognosis predictors of ALL depend on risk-based stratification and the reaction to the initial therapy. Current treatment regimens of ALL contribute to the survival of approximately 80% of pediatric ALL patients with 90% 5-year event-free survival. Based on the patient’s features impacting prognosis and risk of therapy failure, cases can be designated to receive the adequate type of treatment regimens. The higher the risk, the more aggressive, intensive, and toxic is the …

Tecumseh And Tenskwatawa: Myths, Memories, And Messages For Present Times, Matt Hoerauf

Wayne State University Theses

Tecumseh has been hailed as the most famous Indigenous leader in the United States and Canada. Many scholars have bemoaned the difficulty to separating man from myth. One thing is clear: there could be no Tecumseh without his brother Tenskwatawa. It was Tenskwatawa who first had a religious awakening that birthed a spiritual movement. It was Tenskwatawa who was the first leader of this pan-Indigenous group of followers. Tecumseh’s leadership would not emerge until nearly six years later. In the many works on Tecumseh, Tenskwatawa’s story can be easily found. However, he is nearly always portrayed as less important and …

A Patient-Centered Ethnographic Interview And Measurement Of Intelligibility In Spastic-Ataxic Dysarthria For People With Multiple Sclerosis, Courtney Marie Leppek

Wayne State University Theses

Multiple sclerosis (MS) produces neurological impairments that are variable in duration and severity, and that are unique to each case of MS. In addition to variability of symptoms, the length of the MS diagnostic process often affects quality of life. In this mixed qualitative and quantitative study, we used ethnographic interviews to investigate the underlying thoughts and feelings of two very different individuals with MS and Spastic-Ataxic Dysarthria. The results contribute to the understanding of MS as a multi-faceted condition that has implications that are both internal and external to the person with MS. Further, the results reflect the clinical …

The Efficacy Of Curcumin And Oil Palm Phenolics, As Potential Antidepressants, On The Serotonin Pathway In A Neuronal Rat Cell Line, Melanie Hutchings

Wayne State University Theses

Depression can be experienced by individuals of all ages, and can result in alterations of mood, decreased ability to function, and fatigue. Current treatment involves pharmaceuticals, which can result in adverse side effects, and there is a risk of relapse. Nutraceuticals are of interest because they may be able to work as efficiently as current treatments, and will cause fewer adverse effects. Curcumin and oil palm phenolics (OPP) are polyphenolic compounds that exhibit beneficial properties, including anti-oxidant and anti-cancer, and may assist in the regulation of diabetes. For this experiment, we utilized a neuronal rat cell line, RN46A-B14, which exhibits …

Training Effectiveness Analysis Of Osha Silica And Excavation Safety Standards For Construction, Bedel Desruisseaux

Wayne State University Theses

ABSTRACT

TRAINING EFFECTIVENESS ANALYSIS OF

OSHA SILICA AND EXCAVATION SAFETY STANDARDS

FOR CONSTRUCTION

by

BEDEL DESRUISSEAUX

May 2019

Advisor: Dr Mumtaz Usmen

Major: Civil Engineering

Degree: Master of Science

As construction safety and health standards evolve due to changing industry practices and stakeholder expectations, and the mandates get stronger on compliance with these standards, the need for training programs become more pronounced. The proposed paper covers the training material development and program delivery and evaluation efforts associated with two OSHA standards applicable to construction; one on Respirable Crystalline Silica (29 CFR 1926.1153) and other on Excavation and Trenching (29 CFR …

Methods For Classification Of Consumer Review Into The Ones Written Before Or After The Product Purchase, Md Mehedi Hasan

Wayne State University Theses

Online consumer reviews provide a wealth of information about products and services that, if properly identified and extracted, could be of immense value to businesses. While classification of reviews according to sentiment polarity has been extensively studied in previous work, many more focused types of review analysis remain open problems. In this work, we introduce a novel text classification problem of separating post-purchase from pre-purchase consumer review fragments that can facilitate identification of immediate actionable insights based on the feedback from the customers, who actually purchased and own a product. To address this problem, we propose the features, which are …

Carlisle Indian Boarding School Portraits: From The Nineteenth Century To Contemporary Art, Nina Leigh Fiorucci

Wayne State University Theses

This essay examines how Carlisle Indian Boarding School portraits portray transformation in students and the aesthetic history that perception holds with portrait painting, the ideological use of student portraits as illustrations of assimilation, and the continued emotional weight those portraits carry in contemporary media. Formal and aesthetic choices by the official school photographer and propagandistic uses by the school’s founder determine the role of nineteen-century assimilationist and racist ideology in the commission and dissemination of CIBS student portraits. Additionally, the appearance of these images in contemporary media and art provide a continued analysis of CIBS portraits as visual representatives of …

Ros-Based Sensor Fusion And Motion Planning For Autonomous Vehicles: Application To Automated Parkinig System, Yuanzhe Li

Wayne State University Theses

Research and development in autonomous vehicles are currently very active since these vehicles are expected to play an important role in future transportation. During the past decade, numerous Advanced Driver Assistance Systems (ADAS) features have been developed and implemented on production vehicles, such as Adaptive Cruise Control and Lane Keeping Assist. However, most ADAS features are aimed to assist driving on highways where the environments are usually more structured and decision making can be made more easily. Non-highway environments, such as in the case of self-parking, are unstructured and requires more complicated analysis of the image information, localization and path …

Celiac Disease: A Physiological Overview And Possible Future Work With Emphasis On Raman Spectroscopy, Katlyn Curtin Mehne

Wayne State University Theses

Celiac Disease is a condition whereby ingesting Gluten causes an autoimmune reaction in the intestines with adverse effects throughout the body. Although statistic analysis estimates that 1% of the general population is affected by this condition, the diagnosis and treatment research lacks in key areas of understanding. This is a summary of current research and physiological information on the impact of Celiac Disease. In the last twenty years, Raman Spectroscopy assisted in diagnosis and treatment of various other ailments. In this case, Raman Spectroscopy can be used to research immunological cells involved in Celiac Disease and further research.

Functional Analysis Of Bacillus Anthracis Aspartate Transcarbamoylase And Dihydroorotase, Katelyn Leigh Schwager Silva

Wayne State University Theses

There are many enzymes required for efficient and proper pyrimidine biosynthesis. The two that are most important and were discussed in this thesis are aspartate transcarbamoylase (ATCase) and dihydroorotase (DHOase). Both play an important role in not only pyrimidine biosynthesis production, but also mechanistic regulation of de novo synthesis. Anthrax is an infection caused by Bacillus anthracis. Here we studied ATCase and DHOase in Bacillus Anthracis. In this thesis we understood the effects of the enzymes ATCase and DHOase on pyrimidine biosynthesis. Adequate inhibitors of these enzymes would result in cell death and could pose as a cure to infection …

Imbalance Of Uracil Dna Glycosylase And Activation-Induced Cytidine Deaminase Expression In Folate Depleted Human Lymphoblastoids, Elizabeth Zanley

Wayne State University Theses

Background: The DNA base excision repair (BER) pathway is responsible for processing of genomic uracil lesions however, in some tissue types the excisional and gap-filling steps performed by UNG2 and POLβ, respectively, are impaired by folate deficiency in human and murine models in vitro. Genomic uracil damage can be acquired by inadequate conversion of uracil to thymine nucleotide precursors resulting from insufficient folate cofactors, or through activation induced cytosine deaminase (AID) activity during antibody diversification in B-cells in the context of adaptive immunity. The immunoglobulin (Ig) diversification methods in B-cells depend on the coordinated interaction between AID and UNG2, and …

Effect Of Oil Palm Phenolics (Opp) On Pancreatic Cancer Cell Lines, Inaam Abdul Karim

Wayne State University Theses

Pancreatic cancer (paca) is currently the fifth causes of cancer-related deaths in the United States. It’s an aggressive form of cancer with very low survival rates because of delayed diagnosis and limited treatment options. Gemcitabine is the chemotherapy drug that provides minimal benefits along with many side effects. The aim of this study was to examine the in vitro effects of oil palm phenolics (OPP) fraction-5, the water-soluble component of palm oil, in human pancreatic cancer cell models. Two pancreatic cancer cell lines (Panc-1 and BxPC-3) were categorized into control and treatment groups. The control group received cell culture media …