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Characterization Of A Novel Human Cap2 Interacting Protein: Neuronal Calcium Binding Protein 1 (Necab1)., Emilio P. Mottillo
Characterization Of A Novel Human Cap2 Interacting Protein: Neuronal Calcium Binding Protein 1 (Necab1)., Emilio P. Mottillo
Electronic Theses and Dissertations
The actin cytoskeleton is implicated in numerous processes such as cellular division, migration, endocytosislexocytosis, and more recently synaptic plasticity. Important players in controlling cytoskeletal rearrangements are the Cyclase Associated Proteins (CAPs) which are thought to link cell signalling to the dynamics of the actin cytoskeleton, via sequestration of monomeric actin subunits. CAPs are conserved throughout evolution and possess conserved functional domains. Previous studies have suggested a link between CAP proteins and vesicle trafficking and this study focussed on characterizing binding partners of human CAPs. Using a two hybrid screen of a human adult brain cDNA library, a novel human CAP …
Down Regulation Of Gene Expression In Toxoplasma Gondii By Engineered Delta Ribozymes., Ju. Sheng
Down Regulation Of Gene Expression In Toxoplasma Gondii By Engineered Delta Ribozymes., Ju. Sheng
Electronic Theses and Dissertations
Delta ribozyme is a RNA enzyme derived from the genome of hepatitis delta virus (HDV). It can specifically cleave a RNA substrate and is the only ribozyme that can function naturally in human cells. These properties make the delta ribozyme attractive as a gene modulator. Toxoplasma gondii, a pathogenic parasite to humans and pets, has been used for genetic studies. Genes encoding uracil phosphoribosyltransferase (UPRT) and hypoxanthine-xanthine-guanine phosphoribosyltransferase (HXGPRT), which are the operative enzymes of the pyrimidine and purine salvage pathway, respectively, in T. gondii , were chosen for the study of the down regulation effect of delta ribozymes. Different …
Chemical, Biochemical, And Cellular Aspects Of S-Nitrosothiols., Shirin. Akhter
Chemical, Biochemical, And Cellular Aspects Of S-Nitrosothiols., Shirin. Akhter
Electronic Theses and Dissertations
Nitric oxide is involved in many physiological processes including vascular control, immune responses and neurotransmission under physiological conditions. Many functions of NO are triggered by the formation of its metabolite, S-nitrosothiol. S-nitrosothiols play an important role in the delivery, storage and transport of NO. In addition, they regulate the activity of a variety of proteins and enzymes. The first part of my study is focused on studying the regulatory role of S-nitrosothiol. In this study, S-nitrosothiols including S-nitrosoglutathione (GSNO) have been demonstrated to regulate the activity of a key protein in plasma, fibrinogen, without chemically modifying it. GSNO and a …