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University of Nebraska - Lincoln

Biotechnology

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Insights Into The Biotechnology Potential Of Methanosarcina, Sean Carr, Nicole R. Buan Dec 2022

Insights Into The Biotechnology Potential Of Methanosarcina, Sean Carr, Nicole R. Buan

Department of Biochemistry: Faculty Publications

Methanogens are anaerobic archaea which conserve energy by producing methane. Found in nearly every anaerobic environment on earth, methanogens serve important roles in ecology as key organisms of the global carbon cycle, and in industry as a source of renewable biofuels. Environmentally, methanogenic archaea play an essential role in the reintroducing unavailable carbon to the carbon cycle by anaerobically converting low-energy, terminal metabolic degradation products such as one and two-carbon molecules into methane which then returns to the aerobic portion of the carbon cycle. In industry, methanogens are commonly used as an inexpensive source of renewable biofuels as well as …


Methanogen Metabolic Flexibility, Sean Carr Jul 2022

Methanogen Metabolic Flexibility, Sean Carr

School of Biological Sciences: Dissertations, Theses, and Student Research

Methanogens are obligately anaerobic archaea which produce methane as a byproduct of their respiration. They are found across a wide diversity of environments and play an important role in cycling carbon in anaerobic spaces and the removal of harmful fermentation byproducts which would otherwise inhibit other organisms. Methanogens subsist on low-energy substrates which requires them to utilize a highly efficient central metabolism which greatly favors respiratory byproducts over biomass. This metabolic strategy creates high substrate:product conversion ratios which is industrially relevant for the production of biomethane, but may also allow for the production of value-added commodities. Particularly of interest are …


Divergent Evolution Of Extreme Production Of Variant Plant Monounsaturated Fatty Acids, Lu Gan, Kiyoul Park, Jin Chai, Evan M. Updike, Hyojin Kim, Adam Voshall, Sairam Behera, Xiao-Hong Yu, Yuanheng Cai, Chunyu Zhang, Mark A. Wilson, Jeffrey P. Mower, Etsuko Moriyama, Chi Zhang, Sireewan Kaewsuwan, Qun Liu, John Shanklin, Edgar B. Cahoon Jun 2022

Divergent Evolution Of Extreme Production Of Variant Plant Monounsaturated Fatty Acids, Lu Gan, Kiyoul Park, Jin Chai, Evan M. Updike, Hyojin Kim, Adam Voshall, Sairam Behera, Xiao-Hong Yu, Yuanheng Cai, Chunyu Zhang, Mark A. Wilson, Jeffrey P. Mower, Etsuko Moriyama, Chi Zhang, Sireewan Kaewsuwan, Qun Liu, John Shanklin, Edgar B. Cahoon

Center for Plant Science Innovation: Faculty and Staff Publications

Metabolic extremes provide opportunities to understand enzymatic and metabolic plasticity and biotechnological tools for novel biomaterial production. We discovered that seed oils of many Thunbergia species contain up to 92% of the unusual monounsaturated petroselinic acid (18:1Δ6), one of the highest reported levels for a single fatty acid in plants. Supporting the biosynthetic origin of petroselinic acid, we identified a Δ6-stearoyl-acyl carrier protein (18:0-ACP) desaturase from Thunbergia laurifolia, closely related to a previously identified Δ6-palmitoyl-ACP desaturase that produces sapienic acid (16:1Δ6)- rich oils in Thunbergia alata seeds. Guided by a T. laurifolia desaturase crystal structure obtained in this study, …


Genome To Phenome: Improving Animal Health, Production, And Well-Being – A New Usda Blueprint For Animal Genome Research 2018–2027, Caird Rexroad, Jeffrey Vallet, Lakshmi Kumar Matukumalli, James Reecy, Derek Bickhart, Harvey Blackburn, Mark Boggess, Hans Cheng, Archie C. Clutter, Noelle Cockett, Catherine Ernst, Janet E. Fulton, John Liu, Joan Lunney, Holly Neibergs, Catherine Purcell, Timothy P. L. Smith, Tad Sonstegard, Jerry Taylor, Bhanu Telugu, Alison Van Eenennaam, Curtis P. Van Tassell, Kevin Wells May 2019

Genome To Phenome: Improving Animal Health, Production, And Well-Being – A New Usda Blueprint For Animal Genome Research 2018–2027, Caird Rexroad, Jeffrey Vallet, Lakshmi Kumar Matukumalli, James Reecy, Derek Bickhart, Harvey Blackburn, Mark Boggess, Hans Cheng, Archie C. Clutter, Noelle Cockett, Catherine Ernst, Janet E. Fulton, John Liu, Joan Lunney, Holly Neibergs, Catherine Purcell, Timothy P. L. Smith, Tad Sonstegard, Jerry Taylor, Bhanu Telugu, Alison Van Eenennaam, Curtis P. Van Tassell, Kevin Wells

Roman L. Hruska U.S. Meat Animal Research Center: Reports

In 2008, a consortium led by the Agricultural Research Service (ARS) and the National Institute for Food and Agriculture (NIFA) published the “Blueprint for USDA Efforts in Agricultural Animal Genomics 2008–2017,” which served as a guiding document for research and funding in animal genomics. In the decade that followed, many of the goals set forth in the blueprint were accomplished. However, several other goals require further research. In addition, new topics not covered in the original blueprint, which are the result of emerging technologies, require exploration. To develop a new, updated blueprint, ARS and NIFA, along with scientists in the …


Biomaterial Substrate Modifications That Influence Cell-Material Interactions To Prime Cellular Responses To Nonviral Gene Delivery, Amy Mantz, Angela K. Pannier Feb 2019

Biomaterial Substrate Modifications That Influence Cell-Material Interactions To Prime Cellular Responses To Nonviral Gene Delivery, Amy Mantz, Angela K. Pannier

Department of Biological Systems Engineering: Papers and Publications

Gene delivery is the transfer of exogenous genetic material into somatic cells to modify their gene expression, with applications including tissue engineering, regenerative medicine, sensors and diagnostics, and gene therapy. Viral vectors are considered the most effective system to deliver nucleic acids, yet safety concerns and many other disadvantages have resulted in investigations into an alternative option, i.e. nonviral gene delivery. Chemical nonviral gene delivery is typically accomplished by electrostatically complexing cationic lipids or polymers with negatively charged nucleic acids. Unfortunately, nonviral gene delivery suffers from low efficiency due to barriers that impede transfection success, including intracellular processes such as …


Biotechnology Research Pattern In Four Saarc Countries From 2007 To 2016, Manendra Singh Mr. Sep 2018

Biotechnology Research Pattern In Four Saarc Countries From 2007 To 2016, Manendra Singh Mr.

Library Philosophy and Practice (e-journal)

The study presents the trends in authorship pattern and author collaborative in the Biotechnology research field with the sample of 18119 articles which collected from Scopus database for the year 2007 to 2016. The search string used for data download is same for all country and all data downloaded for each country. The three scientometric tools; Collaboration Coefficient, Authorship pattern and Activity Index have been used for the data analysis. The multi- authorship articles are greater than single authorship. The study found that the researchers in Biotechnology move towards team research or group research rather than solo research. The average …


Top 10 Indian Academic/Research Organizations: A Scientometric Analysis Of Research In Biotechnology, Manendra Kumar Singh, Prof. Aditya Tripathi Mar 2018

Top 10 Indian Academic/Research Organizations: A Scientometric Analysis Of Research In Biotechnology, Manendra Kumar Singh, Prof. Aditya Tripathi

Library Philosophy and Practice (e-journal)

Scientometric study is an effective assessment tool for ongoing researches in a given field. It applies mathematical and statistical methods to study the use of documents and patterns of publication. Present work attempts to describe the patterns of publication by top ten Indian Academic/Research Organizations in the field of Biotechnology. Overall, 5423 articles were related to the field in Scopus database during 2001-2016. The applied scientometric tools are Collaboration Coefficient, Co-authorship Index and Activity Index to study the trend of authorship and collaborative research activities in the given domain. The activity Index formula has been modified for the mapping of …


Authorship And Collaboration Pattern In Biotechnology Research: A Study Of Ibsa Countries, Manendra Singh Manendra Sep 2017

Authorship And Collaboration Pattern In Biotechnology Research: A Study Of Ibsa Countries, Manendra Singh Manendra

Library Philosophy and Practice (e-journal)

Scientometric is a type of research method used in the library and information science. The study of the use of documents and patterns of publication in which mathematical and statistical methods have been applied in the scientometric study. It is based on quantitative analysis and statistics to describe patterns of publication within a given field. The study presents the trends of authorship pattern and collaborative research activity in Biotechnology in ISBA Countries. A sample of 24888 articles collect from Scopus database during 2007-2016 is analyzed. The applying scientometric tools are; Collaboration Coefficient, Authorship pattern and Activity Index. In the ten …


Authorship Pattern And Collaboration Coefficient Of India In Biotechnology Research During 2001-2016: Based On Scopus Database, Manendra Kumar Singh May 2017

Authorship Pattern And Collaboration Coefficient Of India In Biotechnology Research During 2001-2016: Based On Scopus Database, Manendra Kumar Singh

Library Philosophy and Practice (e-journal)

The study presents the growth trend about authorship pattern and author collaboration in the Biotechnology for sixteen years with the sample of 18918 articles which have been collected from Scopus database during year 2001-2016. A predefined search string has been used for data download. The five scientometric tools collaboration coefficient (CC), authorship pattern (AP), activity index (AI), Relative Growth Rate (RGR) and Doubling time of Literature (Dt.) have been used for the data analysis. The average number of authors per article for India has been counted as 4.92. In the study, the collaboration coefficient for 16 years (2001-2016) is noticed …


Extreme Weather, Biotechnology, And Corn Productivity, Jonathan R. Mcfadden, John A. Miranowski Jan 2017

Extreme Weather, Biotechnology, And Corn Productivity, Jonathan R. Mcfadden, John A. Miranowski

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

U.S. agriculture has made impressive strides over the past 50 years in crop yield and input productivity growth, especially since the advent of genetically modified crops in 1996. However, future growth rates could decline if U.S. agriculture does not sufficiently adapt to climate change. We examine the magnitudes of weather impacts on U.S. corn yields during 1960–2011—with a focus on intense precipitation and nitrogen use efficiency—and use the empirical results to forecast yields for the subsequent 20 years (2012-2031). We improve upon past methodologies by employing dynamic Bayesian regressions. These dynamic models permit rapid updating of new information, consistent with …


Genome To Phenome: Improving Animal Health, Production, And Well-Being – A New Usda Blueprint For Animal Genome Research 2018–2027, Caird Rexroad, Jeffrey Vallet, Lakshmi Kumar Matukumalli, James Reecy, Derek Bickhart, Harvey Blackburn, Mark Boggess, Hans Cheng, Archie Clutter, Noelle Cockett, Catherine Ernst, Janet E. Fulton, John Liu, Joan Lunney, Holly Neibergs, Catherine Purcell, Timothy P. L. Smith, Tad Sonstegard, Jerry Taylor, Bhanu Telugu, Alison Van Eenennaam, Curtis P. Van Tassell, Kevin Wells Jan 2016

Genome To Phenome: Improving Animal Health, Production, And Well-Being – A New Usda Blueprint For Animal Genome Research 2018–2027, Caird Rexroad, Jeffrey Vallet, Lakshmi Kumar Matukumalli, James Reecy, Derek Bickhart, Harvey Blackburn, Mark Boggess, Hans Cheng, Archie Clutter, Noelle Cockett, Catherine Ernst, Janet E. Fulton, John Liu, Joan Lunney, Holly Neibergs, Catherine Purcell, Timothy P. L. Smith, Tad Sonstegard, Jerry Taylor, Bhanu Telugu, Alison Van Eenennaam, Curtis P. Van Tassell, Kevin Wells

Department of Animal Science: Faculty Publications

In 2008, a consortium led by the Agricultural Research Service (ARS) and the National Institute for Food and Agriculture (NIFA) published the “Blueprint for USDA Efforts in Agricultural Animal Genomics 2008–2017,” which served as a guiding document for research and funding in animal genomics. In the decade that followed, many of the goals set forth in the blueprint were accomplished. However, several other goals require further research. In addition, new topics not covered in the original blueprint, which are the result of emerging technologies, require exploration. To develop a new, updated blueprint, ARS and NIFA, along with scientists in the …


Immunotherapy Using Algal-Produced Ara H 1 Core Domain Suppresses Peanut Allergy In Mice, James A. Grgeory, Ariel Shepley-Mctaggart, Michelle Umpierrez, Barry K. Hurlburt, Soheila J. Maleki, Hugh A. Sampson, Stephen P. Mayfield, M. Cecilia Berin Jan 2016

Immunotherapy Using Algal-Produced Ara H 1 Core Domain Suppresses Peanut Allergy In Mice, James A. Grgeory, Ariel Shepley-Mctaggart, Michelle Umpierrez, Barry K. Hurlburt, Soheila J. Maleki, Hugh A. Sampson, Stephen P. Mayfield, M. Cecilia Berin

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Peanut allergy is an IgE-mediated adverse reaction to a subset of proteins found in peanuts. Immunotherapy aims to desensitize allergic patients through repeated and escalating exposures for several months to years using extracts or flours. The complex mix of proteins and variability between preparations complicates immunotherapy studies. Moreover, peanut immunotherapy is associated with frequent negative side effects and patients are often at risk of allergic reactions once immunotherapy is discontinued. Allergen-specific approaches using recombinant proteins are an attractive alternative because they allow more precise dosing and the opportunity to engineer proteins with improved safety profiles. We tested whether Ara h …


Decline In Ethical Concerns About Reproductive Technologies Among A Representative Sample Of Us Women, Arthur L. Greil, Kathleen S. Slauson-Blevins, Karina M. Shreffler, Katherine M. Johnson, Michele Lowry, Andrea R. Burch, Julia Mcquillan Jan 2016

Decline In Ethical Concerns About Reproductive Technologies Among A Representative Sample Of Us Women, Arthur L. Greil, Kathleen S. Slauson-Blevins, Karina M. Shreffler, Katherine M. Johnson, Michele Lowry, Andrea R. Burch, Julia Mcquillan

Department of Sociology: Faculty Publications

Public awareness and utilization of assisted reproductive technology has been increasing, but little is known about changes in ethical concerns over time. The National Survey of Fertility Barriers, a national, probability-based sample of US women, asked 2031 women the same set of questions about ethical concerns regarding six reproductive technologies on two separate occasions approximately 3 years apart. At Wave 1 (2004–2007), women had more concerns about treatments entailing the involvement of a third party than about treatments that did not. Ethical concerns declined between Wave 1 and Wave 2, but they declined faster for treatments entailing the involvement of …


Sorghum Transformation: Overview And Utility, Tejinder Kumar, Arlene Howe, Shirley Sato, Ismail M. Dweikat, Tom E. Clemente Jan 2013

Sorghum Transformation: Overview And Utility, Tejinder Kumar, Arlene Howe, Shirley Sato, Ismail M. Dweikat, Tom E. Clemente

Department of Agronomy and Horticulture: Faculty Publications

Over the past decade genomics resources available for sorghum have rapidly expanded (Paterson Int J Plant Genomics 2008:6, 2008), these resources, coupled with the recent completion of the genome sequence which is relatively small in size (730 Mb) (Paterson et al. Nature 457:551–556, 2009) makes sorghum a rather attractive species to study. Moreover, the USDA germplasm system maintains 42,614 accessions, of which more than 800 exotic landraces have been converted to day length-insensitive lines to facilitate their use in breeding programs. In addition, a set of EMS mutation stocks developed by the USDA Plant Stress and Germplasm Development Unit in …


Chapter 1 An Introduction To Agricultural Biotechnology Regulation In The U.S., Chris A. Wozniak, Annabel Fellman Waggoner, Sheryl Reilly Jan 2012

Chapter 1 An Introduction To Agricultural Biotechnology Regulation In The U.S., Chris A. Wozniak, Annabel Fellman Waggoner, Sheryl Reilly

United States Environmental Protection Agency: Staff Publications

The regulation of agricultural plant and microbial biotechnology products in the United States of America has a rich history that re fl ects the challenges the federal government has faced in the development of appropriate rules and standards needed to determine their safety to humans and the environment. Several factors – the insuf fi cient global food supply, loss or curtailment of the use of older chemistries to control pests due to risks and environmental persistence, the rising demand for safer food commodities, and the uncertainty surrounding the sustainability of agriculture in this and other countries – have added to …


Strategic Labeling And Trade Of Gmos, Luc Veyssiere, Konstantinos Giannakas Jan 2006

Strategic Labeling And Trade Of Gmos, Luc Veyssiere, Konstantinos Giannakas

Department of Agricultural Economics: Faculty Publications

The emergence of agricultural biotechnology and the subsequent introduction of genetically modified organisms (GMOs) into the food system have been among the most controversial issues surrounding the increasingly scrutinized agri-food system. They have received considerable attention in the economics literature with the main focus being on the optimal regulatory response to products of biotechnology. This paper builds on the literature on the regulation of products of biotechnology by placing the analysis of labeling decisions in a multi-country context. Specifically, the objective of this study is to examine the effect of the strategic interdependence between countries on their regulatory responses to …


Awareness And Acceptance Of Biotechnology Issues Among Youth, Undergraduates, And Adults, Susan Fritz, Dann E. Husmann, Gary Wingenbach, Tracy Rutherford, Valerie Egger, Preeti Wadhwa Dec 2003

Awareness And Acceptance Of Biotechnology Issues Among Youth, Undergraduates, And Adults, Susan Fritz, Dann E. Husmann, Gary Wingenbach, Tracy Rutherford, Valerie Egger, Preeti Wadhwa

Department of Agricultural Leadership, Education, and Communication: Faculty Publications

To be successful both domestically and internationally, biotechnology must establish an acceptable position in the sociopolitical framework. This United States (US) study assessed levels of awareness and acceptance of biotechnology issues among youth (n = 283), undergraduate students (n = 330), and adults (n = 166). The percentage of adults who were aware of how biotechnology would affect food, health, and the environment was almost three times that of youth respondents. It was concluded that consumer groups would most likely be impacted by accurate, unbiased agricultural biotechnology information delivered through the Internet and newspapers that originates from reliable, accessible, and …


Strategic Labeling And Trade Of Gmos, Luc Veyssiere, Konstantinos Giannakas Apr 2003

Strategic Labeling And Trade Of Gmos, Luc Veyssiere, Konstantinos Giannakas

Department of Agricultural Economics: Faculty Publications

The emergence of agricultural biotechnology and the subsequent introduction of genetically modified organisms (GMOs) into the food system have been among the most controversial issues surrounding the increasingly scrutinized agri-food system. They have received considerable attention in the economics literature with the main focus being on the optimal regulatory response to products of biotechnology. This paper builds on the literature on the regulation of products of biotechnology by placing the analysis of labeling decisions in a multi-country context. Specifically, the objective of this study is to examine the effect of the strategic interdependence between countries on their regulatory responses to …


Stability Of Iaa And Iba In Nutrient Medium To Several Tissue Culture Procedures, Scott Nissen, Ellen Sutter Jan 1990

Stability Of Iaa And Iba In Nutrient Medium To Several Tissue Culture Procedures, Scott Nissen, Ellen Sutter

Department of Agronomy and Horticulture: Faculty Publications

The relative stabilities of IAA and IBA under various tissue culture procedures were determined. IBA was significantly more stable than IAA to autoclaving. IBA was also found to be more stable than IAA in liquid Murashige and Skoog medium (MS) under growth chamber conditions. The stabilities of IBA and IAA were similar in agar-solidified MS. Light provided by cool-white fluorescent bulbs promoted degradation of IAA and IBA in both liquid and agar media. Activated charcoal in concentrations as high as 5% was found to adsorb more than 97% of IAA and IBA in liquid MS. These results have important implications …