Digital Commons Network^™

Using Multimedia To Help Agricultural Producers Communicate With Consumers About Gmos, Stacey F. Stearns, Jennifer E. Cushman, Joseph A. Bonelli, Bonnie E. Burr

The Journal of Extension

Genetically modified organisms (GMOs) and their uses are often misunderstood. Consumers are regularly unsure what GMOs are, or if they are safe for consumption and the environment. Contradictory and sometimes inaccurate information is available from numerous sources, and challenges consumers and others to separate the facts from sensationalized stories. Agricultural producers often communicate with consumers, neighbors, and members of the general public; however, they do not have information to share about GMOs. Multimedia resources can provide agricultural producers with science-based information to share with consumers. Agricultural communicators and Extension educators can create science-based multimedia resources to bridge the communication gap.

Emerging Technologies And Terrorism: An American Perspective

Monographs, Collaborative Studies, & IRPs

In a world where technology is rapidly advancing and available to the masses, companies and policymakers face a daunting reality—non-state actors are using innovation for sinister purposes. While artificial intelligence (AI) and autonomous systems promise enhanced threat detection, terrorist groups are exploiting these tools for recruitment and attacks. The future is concerning as AI becomes more widespread and autonomous systems and augmented reality redefine society.

A groundbreaking report is born from a collaboration between NATO COE-DAT and the US Army War College Strategic Studies Institute. This book unveils a grim forecast that terrorists are poised to exploit advances in artificial …

The Impact Of Mutations In The Arabidopsis Apetela (Ap3) Gene, Hazel R. Frans, Tara Phelps-Durr

SACAD: John Heinrichs Scholarly and Creative Activity Days

The purpose of this research is to understand the molecular functioning of the Arabidopsis thaliana Apetela (Ap3) gene. We created mutations in two sites of the gene, AP3-3 and AP3-5. These are predicted to change AP3 protein structure, which may result in a mutated flower. Analyzing the effects of new mutations allows an understanding of protein formation both in plants and humans.

Does Participation Matter? Exploring The Role Of Participation In Corporate Leadership Development Programs On The Intent To Stay Of High-Potential Employees In The Biotech Industry, Glenna M. Griffin

PhD in Organizational Leadership

The idea of leadership development programs for high-potential employees within organizations has long been a standard for driving employee retention within organizations. Yet, employee retention is different from employee intent to stay. Retention metrics measure historical data, looking back to determine how long an employee has stayed in the organization. Employee intent to stay measures, at that moment, data that contribute to an employee’s motivation to stay with the organization. Employers spend significant budgets on leadership development programs for high-potential employees and report on that participation in company retention metrics. Does the investment in these programs also support an employee’s …

Effect Of Black Soldier Fly Larvae And Food Substrates On Weed Seed Emergence, Stephen L. Meyers, Jeanine Arana, Luz Cardona Giraldo, Laura Ingwell, Laura Rodriguez, Nathaly Vargas

Department of Horticulture & Landscape Architecture Faculty Publications

Black soldier fly larvae (Hermetia illucens; BSFL) composting is biotechnology used for organic waste management and an alternative to traditional composting. We designed a two-phase experiment to evaluate the effect of BSFL composting on the emergence of the following six weed species: barnyardgrass (Echinochloa crus-galli), common ragweed (Ambrosia artemisiifolia), giant foxtail (Setaria faberi), ivyleaf morningglory (Ipomoea hederacea), redroot pigweed (Amaranthus retroflexus), and velvetleaf (Abutilon theophrasti). The first experiment phase was in the laboratory (laboratory composting phase), which consisted of 100 seeds of each weed species subjected to five composting treatments [two controls (nontreated and standard Gainesville diet alone) and three …

Genetic Modification – Applications For Forage And Grassland Production Systems, J. R. Caradus

IGC Proceedings (1997-2023)

Genetic modification has been extensively used in crops for 25 years but has rarely been a technology used to advance trait expression in forage and grassland species. The exceptions are the use of herbicide tolerance and low lignin traits in alfalfa which are currently commercially available. In crop species genetic modification has been a valuable option for delivering improved economic and environmental outcomes through providing solutions for many of the challenges facing mankind. There are a many beneficial characteristics that genetic modification has the potential to provide in forage and grassland species. Managing these in the environment and understanding consumer …

Bioactive Molecules From Microalgae, Reham Gamal

Blue Economy

Over the past decade, microalgae screening, particularly cyanobacteria (blue-green algae), for antibiotics and active pharmacological compounds has been of increasing interest. Microalgae are producers of capable of food products, feed supplements, chemicals, and biofuels. Microalgae and marine cyanobacteria are rich in several chemical compounds, therefore; They could be used in some biological applications in relation to health benefits. Microalgae can provide an untapped number of important bioactive molecules (functional components), and their traditional foods (breakfast cereals, spreads, baskets, cookies, brownies, energy bars, mayonnaise, desserts with gels, pastes, emulsions, creams ice and drinks), widely consumed daily, Improves and individuals state of …

Biol 453-002: Applied Genetics And Genomics, Mary Konsolaki

Biology Syllabi

No abstract provided.

Biol 606-102: Applied Bioprocessing & Immunology Based Therapies, John Yarotsky

Biology Syllabi

No abstract provided.

Biol 653-002: Medical Genetics And Genomics, Mary Konsolaki

Biology Syllabi

No abstract provided.

Microbes Of Biotechnological Importance In Acidic Saline Lakes In The Yilgarn Craton, Western Australia, Katelyn Boase, Talitha Santini, Elizabeth Watkin

Research outputs 2022 to 2026

Acidic salt lakes are environments that harbor an array of biologically challenging conditions. Through 16S rRNA, 18S rRNA, and ITS amplicon sequencing of eight such lakes across the Yilgarn Craton of Western Australia, we aim to understand the microbial ecology of these lakes with a focus on iron- and sulfur-oxidizing and reducing microorganisms that have theoretical application in biomining industries. In spite of the biological challenges to life in these lakes, the microbial communities were highly diverse. Redundancy analysis of soil samples revealed sulfur, ammonium, organic carbon, and potassium were significant diversities of the microbial community composition. The most abundant …

Characterization Of Radiotolerance In Potato And Development Of A Gamma Radiation Phytosensor., Robert Graham Sears

Doctoral Dissertations

As humans pursue space travel and nuclear energy, the risk of harm from ionizing radiation increases. On Earth or in space, plants are essential to our personal and environmental health. Plants serve as sentinels, bioremediators and food sources in areas of high ionizing radiation, therefore it is essential to understand how ionizing radiation affects plant biology. This work aimed to understand plant responses to ionizing radiation in the potato chassis and apply that knowledge to generate novel phenotypes for nuclear energy and space applications. The first gamma radiation phytosensor was developed for monitoring at standoff distances greater than three meters. …

A Robust Agroinfiltration Method, Bryce N. Trull

Masters Theses

Stable transformation of soybean (Glycine max) is a markedly slow and laborious process. Thus, a tool that enables rapid evaluation of genetic elements in planta is critical to advance complex research and genetic engineering in soybean. To that end, a substantially robust agroinfiltration method was innovated in this work. Agroinfiltration is a technique that leverages Agrobacterium-mediated transient transformation to deliver genetic elements to the cells of whole plant tissues, usually leaves. Several factors were found to be relevant to successful soybean leaf agroinfiltration, including genotype, surfactant, developmental stage, and Agrobacterium culture medium. The research represents not only …

Plastic Recycling Using Waxworms: Biotechnology Solutions, Lance Wood

Sustainability Conference

As small particles that do not degrade, microplastics harm the environment. Plastics are physically broken down rather than chemically during the traditional recycling process. An insect naturally found in honeybee hives known as the waxworm can break down the most common plastic: polyethylene. A literature review was conducted on a new method to recycle plastics involving the waxworm Galleria mellonella. Literary review studies suggest that recycling using waxworms is more efficient and faster than traditional methods due to oxidation by enzymes. After conducting a literature review, a bioinformatics study was carried out using published DNA and protein sequences. Two promising …

Coexistence Of Specialist And Generalist Species Within Mixed Plastic Derivative-Utilizing Microbial Communities, Laura G. Schaerer, Lindsay I. Putman, Isaac Bigcraft, Emma Byrne, Daniel G. Kulas, Ali Zolghadr, Sulihat Aloba, Rebecca Ong, David Shonnard, Stephen Techtmann

Michigan Tech Publications, Part 2

BACKGROUND Plastic-degrading microbial isolates offer great potential to degrade, transform, and upcycle plastic waste. Tandem chemical and biological processing of plastic wastes has been shown to substantially increase the rates of plastic degradation however, the focus of this work has been almost entirely on microbial isolates (either bioengineered or naturally occurring). We propose that a microbial community has even greater potential for plastic upcycling. A microbial community has greater metabolic diversity to process mixed plastic waste streams and has built-in functional redundancy for optimal resilience. RESULTS Here, we used two plastic-derivative degrading communities as a model system to investigate the …

What's Not Natural Phenomena? Let's Consider A Three-Step Innovative Concept Test For Composition Of Matter Claims, Sydney Hancock

IP Theory

Biotechnology innovation is rapidly growing, especially in the realm of biotech. This growth leads to questions about patent subject matter eligibility of natural phenomena. For example, currently the human genome and microbiome are being extensively studied, bacteriophages are being edited, animals are being cloned, and CRISPR is widespread. Additionally, composition of matter patent claims give the most protection to patent holders. Therefore, knowing when a natural phenomenon veers into human innovation is important for courts, lawyers, and innovators in the era of biotechnology and genetic engineering.

Part I discusses the history of Supreme Court cases on natural phenomena subject matter …

The Code Of Life And Death, Braden R. Leach

Notre Dame Journal on Emerging Technologies

Biotechnology is advancing at an astonishing clip, but our safeguards are decades behind. Given new technologies and economies of scale, it is possible for nefarious actors to assemble deadly viruses from scratch using synthetic DNA ordered off the internet. The Select Agents statute helps to prevent malicious actors from acquiring dangerous pathogens, but the Department of Health and Human Services has interpreted it to not cover synthetic DNA. Recognizing the gap, HHS issued guidance recommending that gene synthesis companies verify their customers to ensure their legitimacy and screen genetic sequences for matches to pathogen sequences. Unsurprisingly, voluntary guidance has not …

Liposome-Based Carriers For Crispr Genome Editing, Xing Yin, Romain Harmancey, David D Mcpherson, Hyunggun Kim, Shao-Ling Huang

Journal Articles

The CRISPR-based genome editing technology, known as clustered regularly interspaced short palindromic repeats (CRISPR), has sparked renewed interest in gene therapy. This interest is accompanied by the development of single-guide RNAs (sgRNAs), which enable the introduction of desired genetic modifications at the targeted site when used alongside the CRISPR components. However, the efficient delivery of CRISPR/Cas remains a challenge. Successful gene editing relies on the development of a delivery strategy that can effectively deliver the CRISPR cargo to the target site. To overcome this obstacle, researchers have extensively explored non-viral, viral, and physical methods for targeted delivery of CRISPR/Cas9 and …

Laboratory Management Models In Core Facilities, Karmen Michael Owen

Theses and Dissertations

Core biotechnology facilities are often defined as laboratories that house shared resources for research. These laboratories are of great importance to research communities because a large portion of scientific research data is generated at these facilities. Different investigators can use core facilities to help further their research. In a university setting, there are several management strategies from small cores that house a specific type of instrument, to large cores that house many different technologies. Funding strategies also differ significantly, from those that are funded by a small group of well-funded investigators, to cores managed by a specific department or school, …

Genome-Wide Association Analysis Identifies Ancestry-Specific Genetic Variation Associated With Acute Response To Metformin And Glipizide In Sugar-Mgh, Josephine H Li, Laura N Brenner, Varinderpal Kaur, Katherine Figueroa, Philip Schroeder, Alicia Huerta-Chagoya, Miriam S Udler, Aaron Leong, Josep M Mercader, Jose C Florez

Journal Articles

AIMS/HYPOTHESIS: Characterisation of genetic variation that influences the response to glucose-lowering medications is instrumental to precision medicine for treatment of type 2 diabetes. The Study to Understand the Genetics of the Acute Response to Metformin and Glipizide in Humans (SUGAR-MGH) examined the acute response to metformin and glipizide in order to identify new pharmacogenetic associations for the response to common glucose-lowering medications in individuals at risk of type 2 diabetes.

METHODS: One thousand participants at risk for type 2 diabetes from diverse ancestries underwent sequential glipizide and metformin challenges. A genome-wide association study was performed using the Illumina Multi-Ethnic Genotyping …

An Assessment Of The Impact Of Raman Based Glucose Feedback Control On Cho Cell Bioreactor Process Development, Luke Gibbons, Francis Maslanka, Nikky Le, Al Magill, Pankaj Singh, Joseph Mclaughlin, Fiona Madden, Ronan Hayes, Barry Mccarthy, Christopher Rode, Jim O'Mahony, Rosemary Rea, Caitlin O'Mahony-Hartnett

Department of Biological Sciences Publications

Process analytical technology (PAT) tools such as Raman Spectroscopy have become established tools for real time measurement of CHO cell bioreactor process variables and are aligned with the QbD approach to manufacturing. These tools can have a significant impact on process development if adopted early, creating an end-to-end PAT/QbD focused process. This study assessed the impact of Raman based feedback control on early and late phase development bioreactors by using a Raman based PLS model and PAT management system to control glucose in two CHO cell line bioreactor processes. The impact was then compared to bioreactor processes which used manual …

Algorithmic Bias: Causes And Effects On Marginalized Communities, Katrina M. Baha

Undergraduate Honors Theses

Individuals from marginalized backgrounds face different healthcare outcomes due to algorithmic bias in the technological healthcare industry. Algorithmic biases, which are the biases that arise from the set of steps used to solve or analyze a problem, are evident when people from marginalized communities use healthcare technology. For example, many pulse oximeters, which are the medical devices used to measure oxygen saturation in the blood, are not able to accurately read people who have darker skin tones. Thus, people with darker skin tones are not able to receive proper health care due to their pulse oximetry data being inaccurate. This …

On The Resurrection Of Microbes: An Eco-Christological Approach To The Resurrection, Denys Janiga Osb

Obsculta

This article attempts to bring ecology and Christology into conversation through a transdisciplinary approach. It looks at the pastoral implications of eco-anxiety, resurrection ecology in the field of biotechnology, Church teaching on the Resurrection, and concludes by initiating an interpretation of the resurrection appearances in the Gospel of Luke through the lens of the microbiome.

Patenting Genetic Information, David S. Olson, Fabrizio Ducci

Indiana Law Journal

The U.S. biotechnology industry got its start and grew to maturity over roughly three decades, beginning in the 1980s. During this period genes were patentable, and many gene patents were granted. University researchers performed basic research— often funded by the government—and then patented the genes they discovered with the encouragement of the Bayh-Dole Act, which sought to encourage practical applications of basic research by allowing patents on federally funded inventions and discoveries. At that time, when a researcher discovered the function of a gene, she could patent it such that no one else could work with that gene in the …

Acclimation And Hardening Of A Slow-Growing Woody Species Emblematic To Western North America From In Vitro Plantlets, Peggy Martinez, Marcelo Serpe, Rachael Barron, Sven Buerki

Biology Faculty Publications and Presentations

Premise: Determining the tolerance of plant populations to climate change requires the development of biotechnological protocols producing genetically identical individuals used for genotype-by-environment experiments. Such protocols are missing for slow-growth, woody plants; to address this gap, this study uses Artemisia tridentata, a western North American keystone shrub, as model.

Methods and Results: The production of individual lines is a two-step process: in vitro propagation under aseptic conditions followed by ex vitro acclimation and hardening. Due to aseptic growth conditions, in vitro plantlets exhibit maladapted phenotypes, and this protocol focuses on presenting an approach promoting morphogenesis for slow-growth, woody species. …

Crispr, Like Any Other Technology: Shedding Determinism & Reviving Athens, Jon Khan

Canadian Journal of Law and Technology

This article examines current narratives surrounding CRISPR (clustered regularly interspaced short palindromic repeats) and the current Canadian treatment of this novel biotechnology. It argues that Canada’s current approach to genetic research and CRISPR appear to have succumbed to the false narrative of technological determinism. It argues that Canada must buck the narrative and alter the current status quo in two principal ways: Canada should pursue more somatic CRISPR clinical trials in humans and permit pre-clinical germline editing. To design a regulatory regime for clinical germline editing and better guidance on somatic CRISPR clinical trials, Canada should engage Deliberative Polling to …

Caveat Venditor: Products Liability And Genetically Modified Foods, Kristopher A. Isham

Journal of Food Law & Policy

Genetically modified organisms (GMOs) have become a lightning rod for conflict between farmers, corporations, shareholders, government agencies, and other concerned groups. Supporters tout GMOs as a solution to the problems of diminishing returns from traditional crop plants and the rising demand for greater quantities of food. Opponents critcize GMOs for potential toxic and allergic reactions in humans, loss of biodiversity, and pesticide and antibiotic resistance in other plants and insects. As the understanding of potential applications of biotechnology broadens, the risks and benefits of such products are being scrutinized more closely.

Rhetorics Of Species Revivalism And Biotechnology – A Roundtable Dialogue, Eva Kasprzycka, Charlotte Wrigley, Adam Searle, Richard Twine

Animal Studies Journal

This informal dialogue contextualises and explores contemporary practices of nonhuman animal gene-modification in de-extinction projects. Looking at recent developments in biotechnology’s role in de-extinction sciences and industries, these interdisciplinary scholars scrutinise the neoliberal impetus driving ‘species revivalism’ in the wake of the Capitalocene. Critical examinations of species integrity, cryo-preservation, techno-optimism, rewilding initiatives and projects aimed at restoring extinct animals such as the woolly mammoth and bucardo are used to map some of the necessary restructuring of conservation policies and enterprises that could secure viably sustainable – and just – futures for nonhuman animals at risk of extinction. The authors question …

Insights Into The Biotechnology Potential Of Methanosarcina, Sean Carr, Nicole R. Buan

Department of Biochemistry: Faculty Publications

Methanogens are anaerobic archaea which conserve energy by producing methane. Found in nearly every anaerobic environment on earth, methanogens serve important roles in ecology as key organisms of the global carbon cycle, and in industry as a source of renewable biofuels. Environmentally, methanogenic archaea play an essential role in the reintroducing unavailable carbon to the carbon cycle by anaerobically converting low-energy, terminal metabolic degradation products such as one and two-carbon molecules into methane which then returns to the aerobic portion of the carbon cycle. In industry, methanogens are commonly used as an inexpensive source of renewable biofuels as well as …

Sui Generis Protection For Plant Varieties And Traditional Knowledge In Biodiversity And Agriculture: The International Framework And National Approaches In The Philippines And India, Christoph Antons

Indian Journal of Law and Technology

The so-called ‘biotechnology clause’ of Article 27.3(b) of the WTO-TRIPS Agreement requires from member states protection for plant varieties either via the patent system or via an ‘effective sui generis system’ or by a combination of the two. Many developing countries prefer forms of sui generis protection, which allow them to include exceptions and protection measures for traditional agricultural practices and the traditional knowledge of farmers and local communities. However, ‘traditional knowledge’ remains a vaguely defined term. Its extension to biodiversity has brought a diffusion of the previously clearer link between protected subject matter, intellectual property and potential beneficiaries. The …