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Analysis Of The N-Glycans Of Recombinant Human Factor Ix Purified From Transgenic Pig Milk, Geun-Cheol Gil, William H. Velander, Kevin E. Van Cott

Department of Chemical and Biomolecular Engineering: Faculty Publications

Glycosylation of recombinant proteins is of particular

importance because it can play significant roles in the

clinical properties of the glycoprotein. In this work, the

N-glycan structures of recombinant human Factor IX (tg-

FIX) produced in the transgenic pig mammary gland were

determined. Themajority of theN-glycans of transgenic pigderived

Factor IX (tg-FIX) are complex, bi-antennary with

one or two terminal N-acetylneuraminic acid (Neu5Ac) moieties.

We also found that the N-glycan structures of tg-FIX

produced in the porcine mammary epithelial cells differed

with respect to N-glycans from glycoproteins produced in

other porcine tissues. tg-FIX contains …

Numerical Simulation Of A Novel Blast Wave Mitigation Device, Zhenbi Su, Wen Peng, Zhaoyan Zhang, George Gogos, Reed Skaggs, Bryan Cheeseman

Department of Mechanical and Materials Engineering: Faculty Publications

This paper proposes a novel blast wave mitigation device, consisting of a piston–cylinder assembly. A shock wave is induced inside the device when it is subject to a blast wave. The shock wave propagates inside the device and is reflected repeatedly. The physical processes within the blast wave mitigation device are simulated numerically. Numerical predictions are in excellent agreement with analytical solutions for special cases of the investigated problem that are available in the literature. The peak pressure on the base of the device caused by the blast wave is studied using a number of design parameters. The numerical simulation …

Fabrication Of High Aspect Ratio Metal Nanotips By Nanosecond Pulse Laser Melting, Bo Cui, Lin Wu, Stephen Y. Chou

Department of Mechanical and Materials Engineering: Faculty Publications

The authors have developed an approach to fabricate sharp and high aspect ratio metal tips using nanosecond pulse laser melting. A quartz wafer covered with a thin chromium (Cr) film was placed on top of a second wafer with a sub-micrometer gap between them and the Cr film facing the second wafer. Then an excimer laser pulse (308 nm wavelength, 20 ns pulse duration) was shone from the back of the quartz wafer and melted the Cr film momentarily (several hundred nanoseconds). It is found that the molten Cr films can self-form discrete metal pillars connecting the two wafers. After …

Conduction In Rectangular Plates With Boundary Temperatures Specified, James V. Beck, Neil T. Wright, A. Haji-Sheikh, Kevin D. Cole, Donald E. Amos

Department of Mechanical and Materials Engineering: Faculty Publications

Steady-state components of heat conduction solutions may have very slowly convergent series for temperatures and non-convergent heat fluxes for temperature boundary conditions. Previous papers have proposed methods to remove these convergence problems. However, even more effective procedures based on insights of Morse and Feshbach are given herein. In some cases it is possible to replace poorly-convergent or non-convergent series by closed-form algebraic solutions. Examples are given.

A Slip Model For Rarefied Gas Flows At Arbitrary Knudsen Number, Lin Wu

Department of Mechanical and Materials Engineering: Faculty Publications

A slip model for wall bounded rarefied gas flows is derived from kinetic theory. A corresponding modified Reynolds lubrication equation is obtained from the slip velocity boundary conditions at walls for high Knudsen number gas flows. The slip model in a simplest form has predictions very close to the numerical solutions of linearized Boltzmann equation in the whole Knudsen number range, and is preferable to the widely applied 1st order (Maxwell slip model), 2nd order, and 1.5 order slip models.

Control Of The Nanoscale Crystallinity And Phase Separation In Polymer Solar Cells, Chih-Wei Chu, Hoichang Yang, Wei-Jen Hou, Jinsong Huang, Gang Li, Yang Yang

Department of Mechanical and Materials Engineering: Faculty Publications

Grazing-incidence x-ray diffraction and atomic force microscopy were performed on bulk heterojunction regioregular poly(3-hexylthiophene) (RR-P3HT) [6,6]-phenyl-C₇₁-butyric acid methyl esters spin-cast films with different film processing conditions to correlate the crystalline nanostructure of P3HT with the corresponding solar cell performance. The increase in long wavelength absorption for solvent annealed films is related to highly conjugated crystal structure of RR-P3HT phase-separated in the active layer. Upon thermal annealing, the solvent annealed 50-nm-thick device shows high solar cell performance with fill factor up to 73% and power conversion efficiency of 3.80%.