Digital Commons Network^™

Characterization Of Calcium Homeostasis Parameters In Trpv3 And Cav3.2 Double Null Mice, Aujan Mehregan

Masters Theses

In mammals, calcium influx is required for oocyte maturation and egg activation, as it supports the persistent calcium oscillations induced by fertilization. These oscillations are required for the initiation of embryo development. The molecular identities of the plasma membrane calcium-permeant channels that underlie calcium influx are not established. Among these channels, Transient Receptor Potential Vanilloid, member 3 (TRPV3) allows divalent cations, namely strontium (Sr²⁺) and calcium (Ca²⁺) with high permeability, into cells, and its expression pattern seems to predict an essential role in the initiation of development. Another channel that was identified to be expressed in …

Regulation Of Katanin Activity On Microtubules, Madison A. Tyler

Masters Theses

The cytoskeleton is a dynamic network of microtubules constantly being reorganized to meet the spatiotemporal demands of the cell. Microtubules are organized into subcellular highways to control cell processes such as cell division, cargo transport, and neuronal development and maintenance. Reorganization of this intricate network is tightly regulated by various stabilizing and destabilizing microtubule-associated proteins that decorate the network. Katanin p60 is a microtubule destabilizing enzyme from the ATPases Associated with various Activities (AAA+) family. It can both sever and depolymerize microtubules. In order to sever microtubules, katanin recognizes the tubulin carboxy-terminal tails (CTTs) and hydrolyzes ATP. Using super-resolution microscopy …

Implications Of Pgrmc1 Regulation Of Kit Ligand Synthesis In The Hippocampus, Haley Woods

Masters Theses

The mammalian hippocampus is responsible for many crucial brain functions such as learning, memory, and neurogenesis in adults. Its degeneration is a pathology associated with the early stages of Alzheimer’s disease. A variety of genes have been associated with both neuroprotection and neurogenesis in the brain, some of which include progesterone membrane component 1 (Pgrmc1) and kit ligand (KitL). Pgrmc1 is recognized for mediating hormonal functions in both the ovary and neuroendocrine regions such as the anteroventral periventricular nucleus (AVPV), but its functions in the hippocampus are not well known. Both Pgrmc1 and KitL share downstream targets, the most strongly …

Uncovering Tasselsheath3. A Genomic And Phenotypic Analysis Of A Maize Floral Mutant., Thompson Zhang

Masters Theses

In the modern era, maize has become the most successful crop grown in the United States. According to the USDA over 90 million acres of land are planted to corn and 96.2% of the U.S feed grain production is made up of the cereal. Part of the success of maize is due to its floral architecture, and its pollination technique in which the flower opens, exposing stamens containing pollen into the air. A unique organ called the lodicule functions as a release mechanism, forcing the flower to open. Lodicules from grasses and eudicot petals are homologous, yet there is little …

Autoplug: An Automated Metadata Service For Smart Outlets, Lurdh Pradeep Reddy Ambati

Masters Theses

Low-cost network-connected smart outlets are now available for monitoring, controlling, and scheduling the energy usage of electrical devices. As a result, such smart outlets are being integrated into automated home management systems, which remotely control them by analyzing and interpreting their data. However, to effectively interpret data and control devices, the system must know the type of device that is plugged into each smart outlet. Existing systems require users to manually input and maintain the outlet metadata that associates a device type with a smart outlet. Such manual operation is time-consuming and error-prone: users must initially inventory all outlet-to-device mappings, …

Labor Union Membership And Black Political Participation, Tiamba Wilkerson

Masters Theses

Despite the connection between labor status and citizenship in Black history, little scholarly consideration has been given to the specific role of labor organizations in Black political participation. This research examines the impact of labor union membership on Black political activity in the immediate post-Civil Rights period, and argues that, similarly to churches and voluntary associations such as the NAACP, labor unions are an important vehicle for political mobilization of the Black community. Results show that Black union members were significantly more likely than non-members to participate in a range of electoral and non-electoral political activities, and to a greater …

The Oxidation State Of Hawaiian Magmas, Michael Vollinger

Masters Theses

In order to estimate the oxygen fugacity of Hawaiian lavas I have measured the ferric/ferrous ratios of samples from the 1984 eruption of Mauna Loa volcano and from the ongoing (1983-2017) Puu Oo eruption of Kilauea volcano. Fifteen samples were studied from the 21 day Mauna Loa eruption and 86 samples, erupted between 1983 and 2004, of the 34 year long Kilauea eruption. Both studies show that, in order to obtain reliable estimates of oxygen fugacity when, where, and how basaltic lava is sampled is of critical importance. Water-quenched lavas and spatter sampled at, or near vents, are less oxidized …

Exploring Long-Term Fault Evolution In Obliquely Loaded Systems Using Tabletop Experiments And Digital Image Correlation Techniques, Kevin Toeneboehn

Masters Theses

This thesis focuses on the use of scaled physical experiments to better understand the development and long-term evolution of fault systems that are otherwise impossible to observe directly. The document is divided into three chapters. The first chapter documents the implementation of an inexpensive stereo vision method for acquiring high resolution three-dimensional strain data for table-top experiments. The second chapter applies the stereo vision method to a tectonic problem—the development of slip partitioning in obliquely loaded crustal systems. Slip partitioned fault systems accommodate oblique convergence with different slip rake on two or more faults and are well documented in the …

How The E. Coli Hsp70 Molecular Chaperone, Dnak, Binds A Client Protein, Joseph Tilitsky

Masters Theses

Protein folding is essential for all cellular life. While some proteins are able to reach their folded state reliably using nothing but their amino acid sequence, a great number of essential proteins are unable to do so without the aid of molecular chaperones. One family of molecular chaperone, the Hsp70 family, is found in virtually all cell types and across all domains of life. Certain to the function of Hsp70s are how they bind their client proteins. Substantial effort has been expended to study how Hsp70s work on model peptides as a substrate mimic, but relatively little work has been …

Analyzing Spark Performance On Spot Instances, Jiannan Tian

Masters Theses

Amazon Spot Instances provide inexpensive service for high-performance computing. With spot instances, it is possible to get at most 90% off as discount in costs by bidding spare Amazon Elastic Computer Cloud (Amazon EC2) instances. In exchange for low cost, spot instances bring the reduced reliability onto the computing environment, because this kind of instance could be revoked abruptly by the providers due to supply and demand, and higher-priority customers are first served.

To achieve high performance on instances with compromised reliability, Spark is applied to run jobs. In this thesis, a wide set of spark experiments are conducted to …

Numerical Simulation Of Multi-Phase Core-Shell Molten Metal Drop Oscillations, Kaushal Sumaria

Masters Theses

The surface tension of liquid metals is an important and scientifically interesting parameter which affects many metallurgical processes such as casting, welding and melt spinning. Conventional methods for measuring surface tension are difficult to use for molten metals above temperatures of 1000 K. Containerless methods are can be used to measure the surface tension of molten metals above 1000 K. Oscillating drop method is one such method where a levitated droplet is allowed to undergo damped oscillations. Using the Rayleigh’s theory for the oscillation of force-free inviscid spherical droplets, surface tension and viscosity of the sample can be calculated from …

Celebrating The Polish Immigrant Community: Strengthening Cultural Bonds And Representation In Westfield, Ma, Alexandra Smialek

Masters Theses

Immigrant communities are a part of every city and town in the United States. Sentiments towards immigrants, however, continue to vary, but in recent years, anti-immigrant sentiments have become more widely encountered, especially because of the recent presidential election (Southern Poverty Law Center, 2016). A lack of knowledge and representation of diverse cultures, along with recurring negative rhetoric, may influence how immigrant populations are received. This report argues that acknowledging culture and heritage can strengthen cultural bonds, create and celebrate a unique city identity, and improve cultural representation. The City of Westfield, Massachusetts, located in Hampden County, will be studied …

Use Of Different Ripening Inhibitors To Enhance Antimicrobial Activity Of Essential Oil Nanoemulsion, Victor Ryu

Masters Theses

The objective of this research was to study the impact of ripening inhibitor level and type on the formation, stability, and activity of antimicrobial thyme oil nanoemulsions formed by spontaneous emulsification. Oil-in-water antimicrobial nanoemulsions (10 wt%) were formed by titrating a mixture of essential oil, ripening inhibitor, and surfactant (Tween 80) into 5mM sodium citrate buffer (pH 3.5). Stable nanoemulsions containing small droplets (d < 70 nm) were formed. The antimicrobial activity of the nanoemulsions decreased with increasing ripening inhibitor concentration, which was attributed to a reduction in the amount of hydrophobic antimicrobial constituents transferred to the separated hydrophobic domain, mimicking bacterial cell membranes, by using dialysis and chromatography. The antimicrobial activity of the nanoemulsions also depended on the nature of the ripening inhibitor used: palm ≈ corn > canola > coconut which also depended on their ability to transfer hydrophobic antimicrobial constituents to the separated hydrophobic domain.

Effects Of Malformed Or Absent Valves To Lymphatic Fluid Transport And Lymphedema In Vivo In Mice, Akshay S. Pujari

Masters Theses

Lymph is primarily composed of fluid and proteins from the blood circulatory system that drain into the space surrounding cells, interstitial space. From the interstitial space, the fluid enters and circulates in the lymphatic system until it is delivered into the venous system. In contrast to the blood circulatory system, the lymphatic system lacks a central pumping organ dictating the predominant driving pressure and velocity of lymph. Transport of lymph via capillaries, pre-collecting and collecting lymphatic vessels relies on the synergy between pressure gradients, local tissue motion, valves and lymphatic vessel contractility. The direction of lymph transport is regulated by …

Social Reproduction In The New England Community College System: A Critical Cultural Studies Perspective, Sarah Marmon

Masters Theses

Statistical data on community colleges confirms how vast the community college institution is: Serving 46% of all undergraduates in the country, or 12.4 million students. A large body of literature exists on the specifics of social reproduction in four-year universities; as well as the specifics of social reproduction in racially and economically segregated high schools. However, there exists a blind spot in this literature when it comes to social reproduction at the community college.

Through conducting interviews with students, faculty and staff at three local community colleges, this ethnographic study explores this theoretical and empirical blind spot by using a …

Transgressive Acts: Adapting Applied Theatre Techniques For A Transgender Community, Theo F. Lefevre

Masters Theses

This MFA Thesis traces my work as a joker (a la Theatre of the Oppressed) and facilitator through a three-year-long project with a trans applied theatre troupe. The troupe explored several techniques, including Image Theatre, Playback Theatre, storytelling exercises, and somatic movement. In three semester-long workshops, the troupe focused work around three sets of techniques. In the first workshop, the troupe explored the community-based interview process of Undesirable Elements, as designed by Ping Chong in collaboration with Talvin Wilks and Sara Zatz. These techniques were interrogated using queer and trans temporalities. In the second unit, the troupe practiced Augusto …

Group Differences In Compassion Fade And Prosocial Behavior, Greg Larsen

Masters Theses

When people learn about a large-scale crisis, they often feel more compassion for its victims when they can fixate on a single, identifiable victim, compared to many victims - a phenomenon known as ‘compassion fade.’ However, throughout the growing compassion fade literature, researchers have mostly given a face to large groups using a single child - a face which may be particularly stirring because children are seen as both likable and incapable of fending for themselves. I conducted two experiments to determine whether the magnitude of this phenomenon varied as a function of characteristics of the victims - namely, how …

Non-Equispaced Fast Fourier Transforms In Turbulence Simulation, Aditya M. Kulkarni

Masters Theses

Fourier pseudo-spectral method on equispaced grid is one of the approaches in turbulence simulation, to compute derivative of discrete data, using fast Fourier Transform (FFT) and gives low dispersion and dissipation errors. In many turbulent flows the dynamically important scales of motion are concentrated in certain regions which requires a coarser grid for higher accuracy. A coarser grid in other regions minimizes the memory requirement. This requires the use of Non-equispaced Fast Fourier Transform (NFFT) to compute the Fourier transform, by solving a system of linear equations.

To achieve similar accuracy, the NFFT needs to return more Fourier coefficients than …

Why Class Matters: Understanding The Relationship Between Class, Family Involvement, And Asian American College Students’ Success, Blair Harrington

Masters Theses

Drawing on intensive interviews with 61 Asian American undergraduates from diverse class and ethnic backgrounds, this paper investigates the relationship between class, family involvement, and student success. I assess three hypotheses derived from the literature. First, social reproduction theorists suggest that parents from advantaged class backgrounds provide more support—economic and cultural capital—to their children than parents from disadvantaged class backgrounds, which leads to greater success for these advantaged offspring. Second, some research challenges this view, arguing instead that class does not impact students’ receipt of support or their resulting success. Third, some now suggest that larger amounts of support may …

Extractable And Non-Extractable Polyphenols From Apples: Potential Anti-Inflammatory Agents, Makenzi Gennette

Masters Theses

With diet being such a huge factor in the development of diseases, emerging research has supported that apple consumption is a promising candidate for disease prevention due to the high phenolic content it possesses. These polyphenols can be found in two forms: extractable polyphenols (EP) and non-extractable polyphenols (NEP). Polyphenols have been shown to have strong anti-inflammatory and anti-oxidant properties, but up until this point, most researchers focus on EP fractions, while NEP are neglected. After the EP extraction using acetone and acetic acid (99:1) from the Apple Boost powder, three additional extraction methods were conducted on the remaining powder …

Energy Efficient Loop Unrolling For Low-Cost Fpgas, Naveen Kumar Dumpala

Masters Theses

Many embedded applications implement block ciphers and sorting and searching algorithms which use multiple loop iterations for computation. These applications often demand low power operation. The power consumption of designs varies with the implementation choices made by designers. The sequential implementation of loop operations consumes minimal area, but latency and clock power are high. Alternatively, loop unrolling causes high glitch power. In this work, we propose a low area overhead approach for unrolling loop iterations that exhibits reduced glitch power. A latch based glitch filter is introduced that reduces the propagation of glitches from one iteration to next. We explore …

Navigation Instruction Validation Tool And Indoor Wayfinding Training System For People With Disabilities, Linlin Ding

Masters Theses

According to World Health Survey, there are 785 million (15.6%) people in the world that live with a disability. It is a well-known fact that lack of access to public transportation is a barrier for people with disabilities in seeking work or accessing health care. In this research, we seek to increase access to public transportation by introducing a virtual pre-travel training system that enables people with disabilities to get familiar with a public transportation venue prior to arriving at the venue. Using this system, users establish a mental map of the target environment prior to their arrival to the …

Juvenile River Herring In Freshwater Lakes: Sampling Approaches For Evaluating Growth And Survival, Matthew T. Devine

Masters Theses

River herring, collectively alewives (Alosa pseudoharengus) and blueback herring (A. aestivalis), have experienced substantial population declines over the past five decades due in large part to overfishing, combined with other sources of mortality, and disrupted access to critical freshwater spawning habitats. Anadromous river herring populations are currently assessed by counting adults in rivers during upstream spawning migrations, but no field-based assessment methods exist for estimating juvenile densities in freshwater nursery habitats. Counts of 4-year-old migrating adults are variable and prevent understanding about how mortality acts on different life stages prior to returning to spawn (e.g., juveniles …

Les Corps Démoniaques Dans La Démonomanie Des Sorciers : Un Examen Ontologique Et Épistémologique, Steven Davis

Masters Theses

The numerous ontological and epistemological paradoxes found within La Démonomanie des sorciers, a demonological treaty of the 16^th century, are studied within the context of demonic corporality: exploiting a rich philosophical and theological intertextuality as well as, more generally, a confessional model of logic, La Démonomanie (1580) constructs a linguistic world of demonic bodies capable of copulation, transformation, and imbuing humans with the power to practice magic. Following in the footsteps of the demonologists who precede him, Bodin constructs a system of the real and of knowledge which is as much dependent upon the authoritative ethos of his …

The Development And Testing Of A Direct Observation Protocol As A Criterion Measure For Children’S Simulated Free-Play Activity, Melanna Cox

Masters Theses

INTRODUCTION: Direct observation (DO) systems have been used for decades to assess free-living PA in children. These traditional DO systems identify the highest intensity observed during alternating observe-and-record periods. Using video-taped DO would allow researchers to code activities and contextual information each time the participant changes their behavior. PURPOSE: To develop and test a novel video-based DO system for children’s free-play activity. METHODS: Following iterative DO system development (The Observer XT, Noldus), 28 children (age=8.4 ± 1.5 years) participated in a 30-minute simulated free-play session that was recorded with a GoPro camera. Participants wore a portable indirect calorimetry (IC) device …

Evaluation Of Impedance Control On A Powered Hip Exoskeleton, Punith Condoor

Masters Theses

This thesis presents an impedance control strategy for a novel powered hip exoskeleton designed to provide partial assistance and leverage the dynamics of human gait. The control strategy is based on impedance control and provides the user assistance as needed which is determined by the user’s interaction with the exoskeleton. A series elastic element is used to drive the exoskeleton and measures the interaction torque between the user and the device. The device operates in two modes. Free mode is a low impedance state that attempts to provide no assistance. Assist mode increases the gains of the controller to provide …

Search Guidance Can Be Adjusted By Experience With Search Discriminability, Junha Chang

Masters Theses

Several recent studies show that previous experience can influence observers’ search strategy in a way that improves search performance. The purpose of the present study is to investigate how the experience of difficult color discriminability affects search strategies. Two participant groups either experienced difficult color discriminability in a half of the trials (i.e., hard-discrimination group) or experienced easy search in all trials (i.e., easy-discrimination group) in a dual-target search task. Participants were required to respond to the presence of a target (colored T) among distractors (colored pseudo-L). Eye movements were recorded to understand which feature information is used to guide …

Influence Of Biomimetic Chelating Packaging On Natural Antimicrobial Efficacy, Paul Castrale

Masters Theses

The iron chelating molecule, ethylenediaminetetraacetic acid (EDTA) is used in food applications for the preservation of oxidation prone ingredients. Research has suggested that EDTA is also capable of enhancing the antimicrobial effectiveness of various compounds including naturally-derived antimicrobials. With consumer demand for cleaner food labels, there remains an opportunity to introduce new chelating technology to replace synthetically-derived EDTA. Through photographting and chemical conversion, hydroxamic acid ligands were covalently bound to polypropylene films resulting in polypropylene-graft-poly(hydroxamic acid) (PP-g-PHA). The resulting films demonstrated an ability to chelate 64 nmol/cm² from an iron saturated environment or 163 nmol/cm …

Partial Craniofacial Cartilage Rescue In Ace/Fgf8 Mutants From Compensatory Signaling From The Ventricle Of Danio Rerio, Douglas A. Calenda Ii

Masters Theses

Examples of asymmetric organs are found throughout the animal kingdom. Whether it is superficial like the fiddler crab’s claw or within an organism like our visceral organs, asymmetries have repeatedly evolved in nature. However, the genetic and developmental origins for asymmetric organ development remain unclear, especially for superficially paired structures. Within zebrafish, a striking example of asymmetry occurs within the ace/fgf8 mutant. The pharyngeal cartilages of these mutants develop asymmetrically 35% of the time, with more cartilages developing on the left or right side of the head, but the origins of this asymmetry are unknown. A significant proportion of mutants …

Validity Of Adhd Symptoms In Toddlers, Hallie Brown

Masters Theses

Attention deficit/hyperactivity disorder (ADHD) is one of the most common child neurodevelopmental disorders. The disorder is typically diagnosed in elementary school, but there is growing evidence for the validity of ADHD in preschoolers, and symptoms likely emerge even earlier than preschool years. Research suggests that ADHD symptoms can be evident in toddlers beyond developmentally appropriate behavior, and symptoms in toddlers are predictive of later impairment. However, few studies have examined the validity of Diagnostic and Statistical Manual of Mental Disorders (DSM) ADHD symptoms in this younger population. The present study examined the 18 DSM ADHD symptoms in a community sample …