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Providing Proteins To Belizean Residents Through Poultry Products, Ellis Freel Dec 2024

Providing Proteins To Belizean Residents Through Poultry Products, Ellis Freel

Poultry Science Undergraduate Honors Theses

Belize is a food insecure, developing country. Although Belize has plenty of nutritious food in-country, the exportation of that food to generate income contributed to a distribution issue of that nutritious food. More impoverished areas of Belize do not have access to nutritious food because of the lack of affordability and high export rates. Diets with a lack of food, or lack of balanced, nutritious food can negatively impact growth and cognitive development, especially in children. Poultry is one of the only products in Belize not able to be exported. Implementing poultry into Belizean diets would affordably alleviate food insecurity …


Mucosal Adjuvants And Delivery Systems, Billy M. Hargis, Neil R. Pumford, Marion Morgan, Srichaitanya Shivaramaiah, Guillermo Tellez-Isaias, Amanda Wolfenden Jan 2024

Mucosal Adjuvants And Delivery Systems, Billy M. Hargis, Neil R. Pumford, Marion Morgan, Srichaitanya Shivaramaiah, Guillermo Tellez-Isaias, Amanda Wolfenden

Patents Granted

Adjuvants comprising chitosan cross-linked with an aldehyde or mannosylated chitosan are provided herein. Methods of making the adjuvants and methods of combining or linking the adjuvants with antigens are also provided. The adjuvant-antigen combinations can be used in vaccine formulations and the vaccine formulations can be used in methods to vaccinate animals against the source of the antigen or to enhance the immune response in a subject.


Process Of Using Arkansas Net Energy Formulations Of Feed Ingredients For Broiler Production, Craig N. Coon, Katie M. Hilton Jan 2023

Process Of Using Arkansas Net Energy Formulations Of Feed Ingredients For Broiler Production, Craig N. Coon, Katie M. Hilton

Patents Granted

This invention relates generally to a process of using Arkansas net energy formulations of feed ingredients for broiler production. In calculating energy requirements, the invention accounts for the heat generated due to maintaining body composition as well as the energy accretion from gain. The process is configured be incorporated into and utilized by a system for formulating feed rations.


Method To Reduce Microbial Bloom In Poultry Hatchery, Ross Wolfenden, Jacob David Lum, Billy M. Hargis, Lisa R. Bielke, Lucas Graham Jul 2022

Method To Reduce Microbial Bloom In Poultry Hatchery, Ross Wolfenden, Jacob David Lum, Billy M. Hargis, Lisa R. Bielke, Lucas Graham

Patents Granted

Spore forming bacteria and Lactic Acid Bacteria for application in poultry hatcher cabinets to alter the bacterial bloom towards a more beneficial microbiota, positively affecting performance parameters such as mortality, body weight gain and feed conversion ratio throughout production.


Yeast Vaccine Vector Including Immunostimulatory And Antigenic Polypeptides And Methods Of Using The Same, Olivia B. Faulkner, Lisa R. Bielke, Leona N. Calhoun, Luc Berghman, Billy M. Hargis Jul 2022

Yeast Vaccine Vector Including Immunostimulatory And Antigenic Polypeptides And Methods Of Using The Same, Olivia B. Faulkner, Lisa R. Bielke, Leona N. Calhoun, Luc Berghman, Billy M. Hargis

Patents Granted

Vaccine compositions including a yeast comprising an immunostimulatory polypeptide and optionally an antigenic polypeptide are provided herein. The immunostimulatory polypeptide and the antigenic polypeptide are expressed or displayed on the surface of the yeast vaccine composition. Methods of using the vaccine composition to vaccinate subjects are also provided.


Compositions And Methods Of Enhancing Immune Responses To Eimeria Or Limiting Eimeria Infection, John Barta, Luc Berghman, Srichaitanya Shivaramaiah, Olivia B. Faulkner, Lisa R. Bielke, Billy M. Hargis Jun 2022

Compositions And Methods Of Enhancing Immune Responses To Eimeria Or Limiting Eimeria Infection, John Barta, Luc Berghman, Srichaitanya Shivaramaiah, Olivia B. Faulkner, Lisa R. Bielke, Billy M. Hargis

Patents Granted

Vaccine vectors and methods of using the vaccine vectors to enhance the immune response to an Apicomplexan parasite and reduce the morbidity or mortality associated with subsequent infection are provided herein. The vaccine vectors include a polynucleotide encoding a Rhomboid polypeptide and optionally include an immune-stimulatory polypeptide suitably expressed on the surface of the vaccine vector.


Compositions And Methods Of Enhancing Immune Responses To Enteric Pathogens, Lisa R. Bielke, Sherryll Layton, Billy M. Hargis, Neil R. Pumford, Olivia B. Faulkner, Luc Berghman, Daad Abi-Ghanem May 2021

Compositions And Methods Of Enhancing Immune Responses To Enteric Pathogens, Lisa R. Bielke, Sherryll Layton, Billy M. Hargis, Neil R. Pumford, Olivia B. Faulkner, Luc Berghman, Daad Abi-Ghanem

Patents Granted

Vaccine vectors capable of eliciting an immune response to enteric bacteria and methods of using the same are provided. The vaccine vectors include a polynucleotide encoding a PAL polypeptide. The PAL polypeptide may be expressed on the surface of the vaccine vector. The vaccine vector may also include a second polypeptide encoding an immunostimulatory polypeptide such as a CD154 polypeptide or an HMGB1 polypeptide.


Vaccine And Methods To Reduce Campylobacter Infection, Billy M. Hargis, Neil R. Pumford, Young M. Kwon, Sherryll Layton Mar 2021

Vaccine And Methods To Reduce Campylobacter Infection, Billy M. Hargis, Neil R. Pumford, Young M. Kwon, Sherryll Layton

Patents Granted

Vaccine vectors and methods for enhancing resistance to Campylobacter infection or for enhancing the immune response to Campylobacter are provided herein. The vaccine vectors include a first polynucleotide which encodes an antigenic polypeptide selected from SEQ ID NO: 7-9 or a fragment thereof. The vector may also include an immunostimulatory polypeptide. The methods include administering the vaccine vectors to a subject.


Compositions, Probiotic Formulations And Methods To Promote Digestion And Improve Nutrition In Poultry, Billy M. Hargis, Guillermo L. Tellez, Juan David Latorre, Ross Wolfenden Mar 2021

Compositions, Probiotic Formulations And Methods To Promote Digestion And Improve Nutrition In Poultry, Billy M. Hargis, Guillermo L. Tellez, Juan David Latorre, Ross Wolfenden

Patents Granted

The present disclosure relates to probiotic compositions, formulations and methods for promoting digestion and improving nutrition in birds, and probiotic compositions and formulations for increasing enzyme production and improving parameters and indications in birds.


Compositions And Methods Of Enhancing Immune Responses To Eimeria, Walter G. Bottje, Billy M. Hargis, Luc Berghman, Young M. Kwon, Kimberly Cole, Mandy Cox, Sherryll Layton, Saeed El-Ashram, John Barta, Guillermo L. Tellez Nov 2020

Compositions And Methods Of Enhancing Immune Responses To Eimeria, Walter G. Bottje, Billy M. Hargis, Luc Berghman, Young M. Kwon, Kimberly Cole, Mandy Cox, Sherryll Layton, Saeed El-Ashram, John Barta, Guillermo L. Tellez

Patents Granted

Vaccines comprising TRAP polypeptides and Salmonella enteritidis vectors comprising TRAP polypeptides are provided. The vaccines may also include a CD154 polypeptide capable of binding to CD40. Also provided are methods of enhancing an immune response against Apicomplexan parasites and methods of reducing morbidity associated with infection with Apicomplexan parasites.


System And Method For Detecting Woody Breast Condition In Broilers Using Image Analysis Of Carcass Features, Casey O. Hanning, Xiao Sun, Juan Pablo Caldas Cueva, Andronikos Mauromostakos Oct 2020

System And Method For Detecting Woody Breast Condition In Broilers Using Image Analysis Of Carcass Features, Casey O. Hanning, Xiao Sun, Juan Pablo Caldas Cueva, Andronikos Mauromostakos

Patents Granted

This invention relates generally to a system and method of detecting woody breast using image analysis of carcass features, and more particularly to a real-time system and method of detecting woody breast in broilers using non-destructive and/or non-contact image analysis of carcass features. The system and method assess woody breast in broilers at the fillet level using image analysis of the angle or area associated with the tip of the keel bone and surrounding breast meat of broiler carcasses. The method is configured be incorporated into and utilized by vision grading system.


Compositions And Methods Of Enhancing Immune Responses To Eimeria Or Limiting Eimeria Infection, John Barta, Luc Berghman, Srichaitanya Shivaramaiah, Olivia B. Faulkner, Lisa R. Bielke, Billy M. Hargis Oct 2020

Compositions And Methods Of Enhancing Immune Responses To Eimeria Or Limiting Eimeria Infection, John Barta, Luc Berghman, Srichaitanya Shivaramaiah, Olivia B. Faulkner, Lisa R. Bielke, Billy M. Hargis

Patents Granted

Vaccine vectors and methods of using the vaccine vectors to enhance the immune response to an Apicomplexan parasite and reduce the morbidity or mortality associated with subsequent infection are provided herein. The vaccine vectors include a polynucleotide encoding a Rhomboid polypeptide and optionally include an immune-stimulatory polypeptide suitably expressed on the surface of the vaccine vector.


Poultry Probiotic Vaccine Compositions And Methods Of Use Thereof, Kyle Dean Teague, Lisa R. Bielke, Guillermo L. Tellez, Billy M. Hargis Sep 2020

Poultry Probiotic Vaccine Compositions And Methods Of Use Thereof, Kyle Dean Teague, Lisa R. Bielke, Guillermo L. Tellez, Billy M. Hargis

Patents Granted

Provided herein are compositions including a probiotic including at least one strain of live bacteria and a Marek's Disease vaccine. Compositions including a probiotic or a probiotic and a Marek's Disease vaccine may be administered to subjects, including poultry. The compositions may be administered in ovo to increase early lactic acid bacteria in the gastrointestinal tract of the subject, to decrease the gram negative bacteria in the gastrointestinal tract of the subject, to reduce the level of Salmonella in the gastrointestinal tract of the subject and to increase the body weight gain of the subject. Also provided are kits including …


Mucosal Adjuvants And Delivery Systems, Billy M. Hargis, Neil R. Pumford, Marion Morgan, Srichaitanya Shivaramaiah, Guillermo L. Tellez, Amanda Wolfenden Sep 2020

Mucosal Adjuvants And Delivery Systems, Billy M. Hargis, Neil R. Pumford, Marion Morgan, Srichaitanya Shivaramaiah, Guillermo L. Tellez, Amanda Wolfenden

Patents Granted

Adjuvants comprising chitosan cross-linked with an aldehyde or mannosylated chitosan are provided herein. Methods of making the adjuvants and methods of combining or linking the adjuvants with antigens are also provided. The adjuvant-antigen combinations can be used in vaccine formulations and the vaccine formulations can be used in methods to vaccinate animals against the source of the antigen or to enhance the immune response in a subject.


Compositions And Methods Of Enhancing Immune Responses To Enteric Pathogens, Lisa R. Bielke, Sherryll Layton, Billy M. Hargis, Neil R. Pumford, Olivia B. Faulkner, Luc Berghman, Daad Abi-Ghanem Jul 2020

Compositions And Methods Of Enhancing Immune Responses To Enteric Pathogens, Lisa R. Bielke, Sherryll Layton, Billy M. Hargis, Neil R. Pumford, Olivia B. Faulkner, Luc Berghman, Daad Abi-Ghanem

Patents Granted

Vaccine vectors capable of eliciting an immune response to enteric bacteria and methods of using the same are provided. The vaccine vectors include a polynucleotide encoding a PAL polypeptide. The PAL polypeptide may be expressed on the surface of the vaccine vector. The vaccine vector may also include a second polypeptide encoding an immunostimulatory polypeptide such as a CD154 polypeptide or an HMGB1 polypeptide.


Yeast Vaccine Vector Including Immunostimulatory And Antigenic Polypeptides And Methods Of Using The Same, Olivia B. Faulkner, Lisa R. Bielke, Leona N. Calhoun, Luc Berghman, Billy M. Hargis Jun 2020

Yeast Vaccine Vector Including Immunostimulatory And Antigenic Polypeptides And Methods Of Using The Same, Olivia B. Faulkner, Lisa R. Bielke, Leona N. Calhoun, Luc Berghman, Billy M. Hargis

Patents Granted

Vaccine compositions including a yeast comprising an immunostimulatory polypeptide and optionally an antigenic polypeptide are provided herein. The immunostimulatory polypeptide and the antigenic polypeptide are expressed or displayed on the surface of the yeast vaccine composition. Methods of using the vaccine composition to vaccinate subjects are also provided.


Compositions And Methods Of Enhancing Immune Responses To Enteric Pathogens, Lisa R. Bielke, Sherryll Layton, Billy M. Hargis, Neil R. Pumford, Olivia B. Faulkner, Luc Berghman, Daad Abi-Ghanem Aug 2019

Compositions And Methods Of Enhancing Immune Responses To Enteric Pathogens, Lisa R. Bielke, Sherryll Layton, Billy M. Hargis, Neil R. Pumford, Olivia B. Faulkner, Luc Berghman, Daad Abi-Ghanem

Patents Granted

Vaccine vectors capable of eliciting an immune response to enteric bacteria and methods of using the same are provided. The vaccine vectors include a polynucleotide encoding a PAL polypeptide. The PAL polypeptide may be expressed on the surface of the vaccine vector. The vaccine vector may also include a second polypeptide encoding an immunostimulatory polypeptide such as a CD154 polypeptide or an HMGB1 polypeptide.


Compositions And Methods Of Enhancing Immune Responses To Eimeria Or Limiting Eimeria Infection, John Barta, Luc Berghman, Srichaitanya Shivaramaiah, Olivia B. Faulkner, Lisa R. Bielke, Billy M. Hargis Jun 2019

Compositions And Methods Of Enhancing Immune Responses To Eimeria Or Limiting Eimeria Infection, John Barta, Luc Berghman, Srichaitanya Shivaramaiah, Olivia B. Faulkner, Lisa R. Bielke, Billy M. Hargis

Patents Granted

Vaccine vectors and methods of using the vaccine vectors to enhance the immune response to an Apicomplexan parasite and reduce the morbidity or mortality associated with subsequent infection are provided herein. The vaccine vectors include a polynucleotide encoding a Rhomboid polypeptide and optionally include an immune-stimulatory polypeptide suitably expressed on the surface of the vaccine vector


Mucosal Adjuvants And Delivery Systems, Billy M. Hargis, Neil R. Pumford, Marion Morgan, Srichaitanya Shivaramaiah, Guillermo L. Tellez, Amanda Wolfenden Apr 2019

Mucosal Adjuvants And Delivery Systems, Billy M. Hargis, Neil R. Pumford, Marion Morgan, Srichaitanya Shivaramaiah, Guillermo L. Tellez, Amanda Wolfenden

Patents Granted

Adjuvants comprising chitosan cross-linked with an aldehyde or mannosylated chitosan are provided herein. Methods of making the adjuvants and methods of combining or linking the adjuvants with antigens are also provided. The adjuvant-antigen combinations can be used in vaccine formulations and the vaccine formulations can be used in methods to vaccinate animals against the source of the antigen or to enhance the immune response in a subject.


Microbiota Metabolic Product Deoxycholic Acid Prevents Campylobacter Jejuni Chicken Colonization Through Modulating Ceca Anaerobes, Bilal Ali Alrubaye Dec 2018

Microbiota Metabolic Product Deoxycholic Acid Prevents Campylobacter Jejuni Chicken Colonization Through Modulating Ceca Anaerobes, Bilal Ali Alrubaye

Graduate Theses and Dissertations

Campylobacter jejuni is a prevalent infectious enteritis mainly foodborne from chickens. Despite of reducing C. jejuni food contamination dramatically decreases campylobacteriosis, few effective approaches are available for the bacterial reduction in chickens. The aim of this study was to use microbial metabolic product deoxycholic acid (DCA) to reduce C. jejuni chicken colonization. Broiler chicks were fed 0 or 1.5 g/kg DCA, lithocholic acid (LCA), or urodeoxycholic acid (UDCA) in diets or orally gavaged with cholic acid (CA, 1.5g/kg body weight). Birds were also transplanted with DCA modulated anaerobes (DCA-Anearo) or aerobes (DCA-Aero). Birds were infected with 109 CFU/bird human clinical …


Compositions And Methods Of Enhancing Immune Responses To Eimeria, Walter G. Bottje, Billy M. Hargis, Luc Berghman, Young M. Kwon, Kimberly Cole, Mandy Cox, Sherryll Layton, Saeed El-Ashram, John R. Barta, Guillermo L. Tellez Jul 2018

Compositions And Methods Of Enhancing Immune Responses To Eimeria, Walter G. Bottje, Billy M. Hargis, Luc Berghman, Young M. Kwon, Kimberly Cole, Mandy Cox, Sherryll Layton, Saeed El-Ashram, John R. Barta, Guillermo L. Tellez

Patents Granted

Vaccines comprising TRAP polypeptides and Salmonella enteritidis vectors comprising TRAP polypeptides are provided. The vaccines may also include a CD154 polypeptide capable of binding to CD40. Also provided are methods of enhancing an immune response against Apicomplexan parasites and methods of reducing morbidity associated with infection with Apicomplexan parasites.


Compositions And Methods Of Enhancing Immune Responses, Walter G. Bottje, Billy M. Hargis, Luc Berghman, Young M. Kwon, Kimberly Cole, Mandy Cox, Sherryll Layton Jun 2018

Compositions And Methods Of Enhancing Immune Responses, Walter G. Bottje, Billy M. Hargis, Luc Berghman, Young M. Kwon, Kimberly Cole, Mandy Cox, Sherryll Layton

Patents Granted

Provided herein are Salmonella enteritidis 13A strains and compositions comprising these strains. Also provided are methods of enhancing an immune response against Influenza A and methods of reducing morbidity associated with an Influenza A infection. Methods of enhancing an immune response to a vaccine vector by expressing a polypeptide of CD154 capable of binding CD40 are also disclosed. Methods of developing a bacterial vaccine vector are disclosed. Methods of generating scarless site-specific mutations in a bacterium are also disclosed.


Vaccine Vectors And Methods Of Enhancing Immune Responses, Luc Berghman, Walter G. Bottje, Billy M. Hargis, Sherryll Layton Mar 2018

Vaccine Vectors And Methods Of Enhancing Immune Responses, Luc Berghman, Walter G. Bottje, Billy M. Hargis, Sherryll Layton

Patents Granted

Provided herein are vaccine vectors including an antigenic polypeptide and an HMGB1 polypeptide present on the surface of the vaccine vector. Compositions comprising the vaccine vectors are also provided and include a pharmaceutically acceptable carrier, suitably a carrier for oral or nasal administration. Also provided are methods of enhancing immune responses, in particular antibody immune response and suitably an IgA response, by administering the vaccine vectors or compositions disclosed herein to a subject.


Compositions And Methods Of Enhancing Immune Responses To Eimeria Or Limiting Eimeria Infection, John Barta, Luc Berghman, Srichaitanya Shivaramaiah, Olivia B. Faulkner, Lisa R. Bielke, Billy M. Hargis Feb 2018

Compositions And Methods Of Enhancing Immune Responses To Eimeria Or Limiting Eimeria Infection, John Barta, Luc Berghman, Srichaitanya Shivaramaiah, Olivia B. Faulkner, Lisa R. Bielke, Billy M. Hargis

Patents Granted

Vaccine, vectors and methods of using the vaccine vectors to enhance the immune response to an Apicomplexan parasite and reduce the morbidity or mortality associated with subsequent infection are provided herein. The vaccine vectors include a polynucleotide encoding a Rhomboid polypeptide and optionally include an immune-stimulatory polypeptide suitably expressed on the surface of the vaccine vector.


Compositions And Methods Of Enhancing Immune Responses To Eimeria Or Limiting Eimeria Infection, John Barta, Luc Berghman, Srichaitanya Shivaramaiah, Olivia B. Faulkner, Lisa R. Bielke, Billy M. Hargis Mar 2017

Compositions And Methods Of Enhancing Immune Responses To Eimeria Or Limiting Eimeria Infection, John Barta, Luc Berghman, Srichaitanya Shivaramaiah, Olivia B. Faulkner, Lisa R. Bielke, Billy M. Hargis

Patents Granted

Vaccine vectors and methods of using the vaccine vectors to enhance the immune response to an Apicomplexan parasite and reduce the morbidity or morality associated with subsequent infection are provided herein. The vaccine vectors include a polynucleotide encoding a Rhomboid polypeptide and optionally include an immune-stimulatory polypeptide suitably expressed on the surface of the vaccine vector.


Compositions And Methods Of Enhancing Immune Responses, Walter G. Bottje, Billy M. Hargis, Luc Berghman, Young M. Kwon, Kimberly Cole, Mandy Cox, Sherryll Layton Jan 2016

Compositions And Methods Of Enhancing Immune Responses, Walter G. Bottje, Billy M. Hargis, Luc Berghman, Young M. Kwon, Kimberly Cole, Mandy Cox, Sherryll Layton

Patents Granted

Provided herein are Salmonella enteritidis 13A strains and compositions comprising these strains. Also provided are methods of enhancing an immune response against Influenza A and methods of reducing morbidity associated with an Influenza A infection. Methods of enhancing an immune response to a vaccine vector by expressing a polypeptide of CD154 capable of binding CD40 are also disclosed. Methods of developing a bacterial vaccine vector are disclosed. Methods of generating scarless site-specific mutations in a bacterium are also disclosed.


Compositions And Methods Of Enhancing Immune Responses To Flagellated Bacterium, Walter G. Bottje, Billy M. Hargis, Luc Berghman, Young M. Kwon, Kimberly Cole, Mandy Cox, Sherryll Layton Sep 2015

Compositions And Methods Of Enhancing Immune Responses To Flagellated Bacterium, Walter G. Bottje, Billy M. Hargis, Luc Berghman, Young M. Kwon, Kimberly Cole, Mandy Cox, Sherryll Layton

Patents Granted

Vaccines comprising fliC and CD 154 polypeptides and Salmonella enteritidis vaccine vectors comprising fliC polypeptides are provided. Also provided arc methods of enhancing an immune response against flagellated bacteria and methods of reducing morbidity associated with infection with flagellated bacteria.


Methods Of Selecting And Using Therapeutic And Prophylactic Probiotic Cultures To Reduce Bacterial Pathogen Loads, Daniel J. Donoghue, Ann M. Donoghue Aug 2015

Methods Of Selecting And Using Therapeutic And Prophylactic Probiotic Cultures To Reduce Bacterial Pathogen Loads, Daniel J. Donoghue, Ann M. Donoghue

Patents Granted

Methods are provided for selecting a bacterium capable of reducing pathogenic bacterial colonization of the intestinal tract in a subject comprising selecting the bacterium capable of migrating at least 0.75 cm from the point of inoculation on motility agar after incubation for 24 hours at 37° C. It is also capable of migrating from the point of inoculation to a diameter of at least 1.5 cm based on the farthest colonies from the point of inoculation on motility agar after incubation for 24 hours at 37° C. Bacteria selected using the method and compositions comprising these bacteria are also provided.


Methods And Compositions Including Spore-Forming Bacteria For Increasing The Health Of Animals, Billy M. Hargis, Ross Wolfenden, Neil R. Pumford, Marion Morgan, Anita Menconi, Guillermo L. Tellez, Amanda Wolfenden, Srichaitanya Shivaramaiah Apr 2015

Methods And Compositions Including Spore-Forming Bacteria For Increasing The Health Of Animals, Billy M. Hargis, Ross Wolfenden, Neil R. Pumford, Marion Morgan, Anita Menconi, Guillermo L. Tellez, Amanda Wolfenden, Srichaitanya Shivaramaiah

Patents Granted

Describe methods, compositions and bacterial isolates for improving the gastrointestinal health of animals and in particular of poultry are provided herein. The methods include administering an endospore-forming bacteria to an animal. The bacteria are selected for the ability to reduce the growth and presence of bacterial pathogens, such as Salmonella, Clostridium, and Campylobacter, in the gastrointestinal tract of the animal. The bacteria are also selected for the ability to improve at least one production parameter in the animal.


Vaccine And Methods To Reduce Campylobacter Infection, Billy M. Hargis, Neil R. Pumford, Young M. Kwon, Sherryll Layton Feb 2015

Vaccine And Methods To Reduce Campylobacter Infection, Billy M. Hargis, Neil R. Pumford, Young M. Kwon, Sherryll Layton

Patents Granted

Describe vaccine vectors and methods for enhancing resistance to Campylobacter infection or for enhancing the immune response to Campylobacter. The vaccine vectors include a first polynucleotide which encodes an antigenic polypeptide selected from SEQ ID NO 7-9 or a fragment thereof. The vector may also include an immunostimulatory polypeptide. The methods include administering the vaccine vectors to a subject.