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Expression Of A Designed Cecropin Analog Gene Using A Baculovirus Vector., Kyusam Choi
Expression Of A Designed Cecropin Analog Gene Using A Baculovirus Vector., Kyusam Choi
LSU Historical Dissertations and Theses
Cecropins are basic, amphipathic peptides with antibacterial activity against many Gram-positive and Gram-negative bacteria. By retaining the positively charged, amphipathic characteristic of cecropin B, a cecropin analog S1, in which 54% of amino acid residues were substituted, was synthesized and shown to possess a broad spectrum of antimicrobial activity. To determine expression of the cecropin analog S1 in Sf9 cells, two gene constructs were designed, and inserted into Autographa californica nuclear polyhedrosis virus under the control of the polyhedrin promoter. One construct, pBS1, consisted of a gene encoding the analog peptide, so that the gene was intended for expression in …
Analysis Of Host-Parasite Cospeciation: Effects Of Spatial And Temporal Scale., James W. Demastes
Analysis Of Host-Parasite Cospeciation: Effects Of Spatial And Temporal Scale., James W. Demastes
LSU Historical Dissertations and Theses
All species of pocket gophers (Geomyidae) are parasitized by at least one species of chewing louse (Trichodectidae), and recent genetic studies have demonstrated that a pattern of cospeciation exists between these hosts and parasites (Hafner and Nadler, 1988; Hafner et al., 1994). Little work has been done on this host-parasite system at lower taxonomic (intrageneric) levels. Although cospeciation is evident in a study of pocket gophers within the genus Geomys and their chewing lice (Geomydoecus), reticulate evolution and retention of ancestral lineages obscure the pattern (Chapter 2). One key to understanding how these patterns of cospeciation are produced and how …
Paleoindicators Of Changing Water Conditions In Louisiana Estuaries., Michael Lewis Parsons
Paleoindicators Of Changing Water Conditions In Louisiana Estuaries., Michael Lewis Parsons
LSU Historical Dissertations and Theses
Sediment cores were collected to study the development of eutrophication and hydrological changes in coastal Louisiana. The cores were taken at marsh sites hypothesized to be influenced primarily by riverine inputs (Fourleague Bay-FLB), localized activities (Terrebonne Bay-TSMP), and both riverine and localized processes (Airplane Lake-APL). The sediment cores represented time frames ranging from 330 years (APL) to 70 years (TSMP) BP according to core dating results. Sediment core samples were analyzed for diatoms, other microfossils, pigments, biogenic silica, and sediment characteristics. Additionally, land use records were utilized as a proxy of anthropogenic activities, and to aid in the interpretation of …
Characterization Of Physiological Changes In Roots Grown In Spaceflight Conditions: A Comparison Of Nutrient Delivery Technologies., David Marshall Porterfield
Characterization Of Physiological Changes In Roots Grown In Spaceflight Conditions: A Comparison Of Nutrient Delivery Technologies., David Marshall Porterfield
LSU Historical Dissertations and Theses
The best strategy for providing food and maintaining the environment on long-duration space missions is a bioregenerative life support system based on the growth of higher plants. Before such a system can be implemented, a better understanding of plant growth in space will have to be achieved. Little is known about the role of gravity-dependent physical processes in normal physiological function. A series of ground-based and spaceflight experiments was conducted to examine root oxygen availability in microgravity nutrient delivery systems. In spaceflight experiments Arabidopsis thaliana (L.) Heynh. plants were analyzed for changes in root medium redox potential and root alcohol …