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Investigations On The Essentiality Of The Escherichia Coli Deda Membrane Protein Family, Lisa A. Boughner
Investigations On The Essentiality Of The Escherichia Coli Deda Membrane Protein Family, Lisa A. Boughner
LSU Doctoral Dissertations
The DedA protein family is a highly conserved family of membrane proteins, with members present throughout all domains of life: Bacteria, Archaea, and Eukarya. Investigation of the DedA membrane protein family began with the isolation of BC202, an Escherichia coli mutant with in-frame deletions of two DedA proteins of unknown function (YqjA and YghB) that share 61% amino acid identity. BC202 demonstrates temperature sensitivity, inefficient cell division, an altered phospholipid composition, increased expression of extracytoplasmic stress response pathways, as well as an inability to maintain the cell membrane proton motive force (PMF). Additionally, Borrelia burgdorferi has a single DedA homolog …
Marr Family Transcriptional Regulators From Streptomyces Coelicolor, Hao Huang
Marr Family Transcriptional Regulators From Streptomyces Coelicolor, Hao Huang
LSU Doctoral Dissertations
A family of transcriptional regulators that ubiquitously exists in prokaryotes is the multiple antibiotic resistance regulator (MarR) family. These transcriptional regulators participate in many cellular processes and can provide valuable knowledge about transcriptional regulation in response to specific conditions. The closely related MarR homologs TamR (trans-aconitate methyltransferase regulator) and PecS in Streptomyces coelicolor were studied to investigate their potential role in this bacterium. In Streptomyces coelicolor, the gene (SCO3133), which encodes TamR, is oriented divergently from the tam gene, which encodes trans-aconitate methyltransferase. TamR was found to regulate several target genes, which encode several enzymes closely related to the citric …
Diversity And Activity Of Aerobic Thermophilic Carbon Monoxide-Oxidizing Bacteria On Kilauea Volcano, Hawaii, Caitlin Elizabeth King
Diversity And Activity Of Aerobic Thermophilic Carbon Monoxide-Oxidizing Bacteria On Kilauea Volcano, Hawaii, Caitlin Elizabeth King
LSU Doctoral Dissertations
Aerobic carbon monoxide (CO) oxidation is used by phylogenetically and physiologically diverse microorganisms inhabiting a variety of terrestrial and aquatic ecosystems. Activity assays, culture-based studies, and molecular-based approaches targeting the coxL gene, encoding the large subunit of CO dehydrogenase, were used to investigate the role of temperature in structuring CO-oxidizing communities at Kilauea Volcano, Hawai’i. CO uptake activity was assessed for unvegetated and vegetated temperate volcanic deposits that experience different temperature regimes due to plant development during ecosystem succession. Both CO-oxidizing communities had similar short-term responses to temperature; however, results from extended incubations (30 d) at elevated temperature (55 °C) …
Role Of Nod2/Rip2 Signaling In Acute Bacterial Pneumonia And Sepsis, Balamayooran Theivanthiran
Role Of Nod2/Rip2 Signaling In Acute Bacterial Pneumonia And Sepsis, Balamayooran Theivanthiran
LSU Doctoral Dissertations
Bacterial pneumonia and sepsis are two important causes of mortality in the world. Emergence of multidrug resistant bacteria has necessitated the development of new treatment and/or prevention strategies to augment host immune defense. In this context, the innate host defense is critical in clearing pathogenic bacteria from the host. Early neutrophil recruitment is a critical step in a multistep requence leading to bacterial clearance. Pattern recognition receptors (PRRs) play a critical role in the innate immune system. Receptor interacting protein 2 (RIP-2) is an adaptor for the nod-like receptors (NLR) NOD1 and NOD2. Nucleotide oligomerisation domain 2 (NOD2) is an …