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High-Pressure Effects On Lysosome Integrity And Lysosomal Enzyme Activity In Bovine Muscle, Stéphanie Jung, Marie De Lamballerie-Anton, Richard G. Taylor, Mohamed Ghoul

Stéphanie Jung

This study was conducted to determine whether the application of high hydrostatic pressure could modify the enzymatic activity and membrane integrity of lysosomes in muscle. Several combinations of pressure (0-600 MPa) and time (0-300 s) were applied to two types of samples: purified enzymes (cathepsin D and acid phosphatase) in buffer solution and intact muscle (biceps femoris). The enzymes studied showed varying degrees of susceptibility depending on the level of pressure, holding time, and environment. Acid phosphatase activity was minimally affected by pressure in buffer solution, whereas cathepsin D was modulated significantly by the pressure and time applied. The activities …