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Determination Of 2-Alkylcyclobutanones With Electronic Impact And Chemical Ionization Gas Chromatography/Mass Spectrometry (Gc/Ms) In Irradiated Foods, Peter Horvatovich, Dalal Werner, Stéphanie Jung, Michel Miesch, Henry Delincee, Claude Hasselmann, Eric Marchioni
Determination Of 2-Alkylcyclobutanones With Electronic Impact And Chemical Ionization Gas Chromatography/Mass Spectrometry (Gc/Ms) In Irradiated Foods, Peter Horvatovich, Dalal Werner, Stéphanie Jung, Michel Miesch, Henry Delincee, Claude Hasselmann, Eric Marchioni
Stéphanie Jung
The use of a column containing 60 g of silica gel for cleanup and the use of isobutane as a reactant reagent for chemical ionization-mass spectrometric analysis of the saturated and monounsaturated alkyl side-chain 2-alkylcyclobutanones (2-ACBs; specifically induced by irradiation from fat in foods until the proof of contrary) has improved both the sensibility and the selectivity of the method when applied for the detection of irradiated foods. The quality of the chromatograms obtained was improved, allowing the detection of food samples (avocados) irradiated at low doses (0.1 kGy) or irradiated ingredients included in low proportions (less than 5%, wt/wt) …
High-Pressure Effects On Lysosome Integrity And Lysosomal Enzyme Activity In Bovine Muscle, Stéphanie Jung, Marie De Lamballerie-Anton, Richard G. Taylor, Mohamed Ghoul
High-Pressure Effects On Lysosome Integrity And Lysosomal Enzyme Activity In Bovine Muscle, Stéphanie Jung, Marie De Lamballerie-Anton, Richard G. Taylor, Mohamed Ghoul
Stéphanie Jung
This study was conducted to determine whether the application of high hydrostatic pressure could modify the enzymatic activity and membrane integrity of lysosomes in muscle. Several combinations of pressure (0-600 MPa) and time (0-300 s) were applied to two types of samples: purified enzymes (cathepsin D and acid phosphatase) in buffer solution and intact muscle (biceps femoris). The enzymes studied showed varying degrees of susceptibility depending on the level of pressure, holding time, and environment. Acid phosphatase activity was minimally affected by pressure in buffer solution, whereas cathepsin D was modulated significantly by the pressure and time applied. The activities …