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The Prevalence Of The Q-Fever Agent Coxiella Burnetii In Ticks Collected From An Animal Shelter In Southeast Georgia, John H. Smoyer Iii Jan 2004

The Prevalence Of The Q-Fever Agent Coxiella Burnetii In Ticks Collected From An Animal Shelter In Southeast Georgia, John H. Smoyer Iii

Electronic Theses and Dissertations

Author's abstract: Q-fever is a zoonosis caused by a worldwide-distributed bacterium Coxiella burnetii. Ticks are vectors of the Q-fever agent but play a secondary role in transmission because the agent is also transmitted via aerosols. Most Q-fever studies have focused on farm animals but not ticks collected from dogs in animal shelters. In order to detect the Q-fever agent in these ticks, a nested PCR technique targeting the 16S rDNA of Coxiella burnetii was used. A collection of 450 ticks from the animal shelter were screened via nested PCR and 144 (32%) were positives. The positive PCR products were also …


Population Genetics Analysis Of The Grass Shrimp Palaemonetes Pugio Using Single Strand Conformation Polymorphism, Melody A. Flowers Jan 2004

Population Genetics Analysis Of The Grass Shrimp Palaemonetes Pugio Using Single Strand Conformation Polymorphism, Melody A. Flowers

Electronic Theses and Dissertations

Little is known about the genetic structure of grass shrimp, Palaemonetes pugio, populations or the effects the parasite Microphallus turgidus has on it. The objective of this study was to determine the amount of genetic diversity of the shrimp populations based on locality and parasite load. In order to examine the genetics of P. pugio, shrimp DNA was extracted and scanned using single stand conformation polymorphism. Results revealed P. pugio populations are highly conserved among localities. This suggests a significant amount of gene flow is taking place. Results also indicate there is no correlation between the most common host haplotype …