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Molecular Approaches To Detect And Control Cercospora Kikuchii In Soybeans, Ashok Kumar Chanda
Molecular Approaches To Detect And Control Cercospora Kikuchii In Soybeans, Ashok Kumar Chanda
LSU Doctoral Dissertations
Cercospora leaf blight (CLB) caused by Cercospora kikuchii, has become a troublesome disease in the southern United States. C. kikuchii produces a non-hostspecific phytotoxin and a pathogenicity factor known as cercosporin during infection of soybean. A quantitative real-time PCR assay was developed for detection and quantification of C. kikuchii. The sensitivity of detection is 1 pg of genomic DNA. The assay detected the presence of C. kikuchii in soybean leaves long before the appearance of disease symptoms. C. kikuchii DNA levels in soybean leaves increased slowly during early soybean development, followed by a quick increase at late reproductive stages. Results …