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A Survey Of Ballistosporic Phylloplane Yeasts In Baton Rouge, Louisiana, Sebastian Albu
A Survey Of Ballistosporic Phylloplane Yeasts In Baton Rouge, Louisiana, Sebastian Albu
LSU Master's Theses
A study documenting basidiomycete yeast biodiversity was conducted in Baton Rouge, Louisiana during 2010 and 2011. Using the spore-fall method, the leaf surfaces of seven ferns were sampled at biweekly intervals. Maximum likelihood phylogenetic analyses of the internal transcribed spacer (ITS) region and the D1/D2 domain of the large subunit (LSU) of nuclear ribosomal DNA were used to identify 463 isolates representing 81 species spanning 12 orders within six classes in Basidiomycota. Nearly 30 of these isolates appear to be species new to science. Data indicate fern leaf developmental stage has an effect on the number of yeasts present. A …
Comparative Genomics, Transcriptome Analysis And Characterization Of Selected Regulatory Genes Of Burkholderia Glumae, Felix Francis
Comparative Genomics, Transcriptome Analysis And Characterization Of Selected Regulatory Genes Of Burkholderia Glumae, Felix Francis
LSU Master's Theses
Burkholderia glumae is the primary causal agent of bacterial panicle blight of rice, which is becoming a major threat to global rice production. The genome of a highly virulent B. glumae strain, 336gr-1 that was isolated from rice in Louisiana, was sequenced to better understand the genome-scale characteristics, particularly that of its pathogenicity. Comparative genomic analyses with another strain, BGR1 that was isolated from Korea, revealed several unique regions present in the genomes of these two geographically separated phytopathogenic bacteria. Genome plasticity, primarily caused by a horizontal gene transfer, was observed in these closely related strains of Burkholderia that are …
Dynamics Of The Sweetpotato Potyvirus Aphid Pathosystem In Louisiana, Everlyne Nafula Wosula
Dynamics Of The Sweetpotato Potyvirus Aphid Pathosystem In Louisiana, Everlyne Nafula Wosula
LSU Doctoral Dissertations
Sweetpotato potyviruses [Sweet potato feathery mottle virus (SPFMV), Sweet potato virus G (SPVG) and Sweet potato virus 2 (SPV2)] commonly infect sweetpotato and weedy morning glories in the USA. These viruses are transmitted in a non-persistent manner by various aphid species and cause up to 15% yield loss. Sweetpotato is vegetatively propagated, and in the USA growers are supplied with virus tested propagation material to minimize impact of viruses. However the rapid re-infection of these materials with viruses warranted further studies to determine factors that influence the epidemiology of these viruses. The objectives of this study were: (i) to determine …
Molecular Approaches To Detect And Control Cercospora Kikuchii In Soybeans, Ashok Kumar Chanda
Molecular Approaches To Detect And Control Cercospora Kikuchii In Soybeans, Ashok Kumar Chanda
LSU Doctoral Dissertations
Cercospora leaf blight (CLB) caused by Cercospora kikuchii, has become a troublesome disease in the southern United States. C. kikuchii produces a non-hostspecific phytotoxin and a pathogenicity factor known as cercosporin during infection of soybean. A quantitative real-time PCR assay was developed for detection and quantification of C. kikuchii. The sensitivity of detection is 1 pg of genomic DNA. The assay detected the presence of C. kikuchii in soybean leaves long before the appearance of disease symptoms. C. kikuchii DNA levels in soybean leaves increased slowly during early soybean development, followed by a quick increase at late reproductive stages. Results …
Searching For Alternative Hosts And Determining The Variation In The Internal Transcribed Spacer (Its) Region Of Phakopsora Pachyrhizi And The Implications For Currently Used Molecular Diagnostic Assays: Subtitle, Tomas Allen Rush
LSU Master's Theses
Phakopsora pachyrhizi, the causal agent of soybean rust (SBR), is a serious disease on soybeans.The objectives of this project were to identify additional alternative and possible overwintering hosts of the SBR pathogen and to validate the current detection assays for SBR. For the first objective, we attempted to identify naturalized Louisiana legume(s) that can serve as hosts and overwintering sites for P. pachyrhizi. It was theorized that New Iberia and Iberville Parishes in south Louisiana are usually the first locations of soybean infection in the spring. This suggests that there may be alternative leguminous hosts located in these parishes that …