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Identification Of Micro-Rnas And Their Messenger Rna Targets In Prostate Cancer And Biological Fluids, Kanika Sharma
Identification Of Micro-Rnas And Their Messenger Rna Targets In Prostate Cancer And Biological Fluids, Kanika Sharma
Theses and Dissertations
Prostate cancer is the second most common cancer in the United States that affects men today. To better treat this disease accurate biomarkers and successful therapeutic treatments are needed. A novel approach to understand the mechanisms behind prostate cancer tumor formation lies in identifying dysregulated micro-RNAs (miRNAs), which are a class of small (18-24 nucleotides) non-coding RNAs that regulate gene expression posttranscriptionally by either inhibiting protein synthesis or signaling messenger-RNA for degradation. Multiple miRNAs were discovered in our highly tumorigenic and metastatic prostate cancer progression model M12 cell line compared to its weakly tumorigenic P69 parental cell line. Various analyses …
A Multifaceted Approach Identifies Erbb2 And Erbb3 Proteins And Microrna-125b As Key Contributors To Prostate Cancer Progression, Danielle Weaver
A Multifaceted Approach Identifies Erbb2 And Erbb3 Proteins And Microrna-125b As Key Contributors To Prostate Cancer Progression, Danielle Weaver
Theses and Dissertations
Prostate cancer is the most common cancer affecting men today. Therefore, there is a strong need for accurate biomarkers and successful therapeutic treatments. A novel approach combining a computationally built protein-protein interaction network of proven microRNA protein targets with high throughput proteomics identified ErbB2 and ErbB3 as key proteins in prostate cancer. These results coupled with microRNA array screening of an androgen-independent prostate cancer progression model, substantiated by single microRNA analysis, suggested miR125b as a key tumor suppressor contributing to prostate cancer progression. miR125b expression was shown to be substantially increased in the non-tumorigenic P69 cell line compared to its …