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A Public–Industry Partnership For Enhancing Corn Nitrogen Research And Datasets: Project Description, Methodology, And Outcomes, Newell R. Kitchen, John F. Shanahan, Curtis J. Ransom, Christopher J. Bandura, Gregory M. Bean, James J. Camberato, Paul R. Carter, Jason D. Clark, Richard B. Ferguson, Fabian G. Fernandez, David W. Franzen, Carrie A.M. Laboski, Emerson D. Nafziger, Zhisheng Qing, John E. Sawyer, Matt Shafer

Jason Clark

Due to economic and environmental consequences of N lost from fertilizer applications in corn (Zea mays L.), considerable public and industry attention has been devoted to the development of N decision tools. Needed are research and databases and associated metadata, at numerous locations and years to represent a wide geographic range of soil and weather scenarios, for evaluating tool performance. The goals of this research were to conduct standardized corn N rate response field studies to evaluate the performance of multiple public-domain N decision tools across diverse soils and environmental conditions, develop and publish new agronomic science for improved …

Regenerating Agricultural Landscapes With Perennial Groundcover For Intensive Crop Production, Kenneth J. Moore, Robert P. Anex, Amani E. Elobeid, Shuizhang Fei, Cornelia B. Flora, A. Susana Goggi, Keri L. Jacobs, Prashant Jha, Amy L. Kaleita, Douglas L. Karlen, David A. Laird, Andrew W. Lenssen, Thomas Lubberstedt, Marshall D. Mcdaniel, D. Raj Raman, Sharon L. Weyers

Douglas L Karlen

The Midwestern U.S. landscape is one of the most highly altered and intensively managed ecosystems in the country. The predominant crops grown are maize (Zea mays L.) and soybean [Glycine max (L.) Merr]. They are typically grown as monocrops in a simple yearly rotation or with multiple years of maize (2 to 3) followed by a single year of soybean. This system is highly productive because the crops and management systems have been well adapted to the regional growing conditions through substantial public and private investment. Furthermore, markets and supporting infrastructure are highly developed for both crops. As maize and …

Understanding Anhydrous Ammonia Application In Soil, John E. Sawyer

John E. Sawyer

What happens when anhydrous ammonia is injected into soil?

Several physical and chemical reactions take place following anhydrous ammonia injection: dissolution in water, reaction with soil organic matter and clay, and attachment of ammonium ions on the soil cation exchange complex. These reactions all tend to limit the movement of ammonia, with water having the greatest initial effect. The highest concentration of ammonia is at/near the point of injection, with a tapering of the concentration toward the outer edge of the retention zone. Usually the greatest ammonia concentration is within the first inch or two of the injection point, with …

Flood Adulterated Grain - Nutrient Supply To Crops When Land Applied, John E. Sawyer, Antonio Mallarino

John E. Sawyer

Flooding has been extensive in several areas of Iowa this spring. In some cases, stored grain has been affected by flood waters. Land application of flood adulterated grain as a nutrient source for a future crop may be an option for some. See the Iowa Dept. of Natural Resources (IDNR) and Iowa Dept. of Agriculture and Land Stewardship (IDALS) publication for Proper Management of Flooded Grain and Hay. That publication states “Farms disposing of spoiled grain and feed on their own property can land apply damaged grain at the following application rates: 146 bu corn/acre and 50 bu soybean/acre”. …

Anhydrous Ammonia Application -- Spring 2019, John E. Sawyer

John E. Sawyer

It was a late harvest in fall 2018. Soils were wet and frozen when it was time to apply anhydrous ammonia. Those situations resulted in much less than normal anhydrous ammonia application last fall. Therefore, considerable anhydrous ammonia needs to be applied this spring. There is only so much capacity to switch from one nitrogen (N) fertilizer product to another. In Iowa, historically the two largest N fertilizers are anhydrous ammonia (largest) and urea-ammonium nitrate (UAN) solution, with granulated urea a distant third.

Springtime Precipitation – A Tool For Estimating Nitrogen Application Need For Corn, John E. Sawyer

John E. Sawyer

Wet springtime conditions typically raise questions about the status of applied nitrogen (N). Or the question, do I need to apply additional N to my cornfields? At this time we do not know what the weather/precipitation will be for the entire springtime, but the extended period of wet conditions this spring is of concern. A summary of long-term N rate trials with continuous corn and corn rotated with soybean conducted at seven ISU research and demonstration farms in Iowa from 1999 to 2016 has been an on-going basis for indicating the potential for needing an N rate greater than the …

Nitrogen Fertilization Options With Delayed Or Rescue Applications, John E. Sawyer

John E. Sawyer

It’s been a wait-go-stop (repeat) corn planting season this spring. Whether you planted early or are just now getting corn planted, it seems planting windows were short and rushed. In some cases this meant planting (corn/soybean) and worrying later about getting nitrogen (N) applied. And in some areas of Iowa, wetter than normal conditions are raising questions about supplemental N application. What are the options for sidedress N?

Effects Of Unharvested, Shattered, Or Hailed-Out Soybean Fields On Nutrient Supply For Corn, John E. Sawyer

John E. Sawyer

Flooded fields and wet soil conditions in the fall 2018 meant some soybean fields were not, or are not going to be harvested. Also, some fields occasionally experienced significant shattering or a hailstorm in the fall where soybean seed is knocked from the plants and thus not harvested. When the grain is not harvested, nitrogen (N), phosphorus (P), and potassium (K) nutrients returned to the soil in the soybean grain can be accounted for when planning nutrient applications for the next crop. Other nutrients will also be returned, but most are not a fertilization need in Iowa soils. An estimate …

Parallel Genome-Wide Expression Profiling Of Host And Pathogen During Soybean Cyst Nematode Infection Of Soybean, Nagabhushana Ithal, Justin Recknor, Dan Nettleton, Leonard Hearne, Tom Maier, Thomas J. Baum, Melissa G. Mitchum

Dan Nettleton

Global analysis of gene expression changes in soybean (Glycine max) and Heterodera glycines (soybean cyst nematode [SCN]) during the course of infection in a compatible interaction was performed using the Affymetrix GeneChip soybean genome array. Among 35,611 soybean transcripts monitored, we identified 429 genes that showed statistically significant differential expression between uninfected and nematode-infected root tissues. These included genes encoding enzymes involved in primary metabolism; biosynthesis of phenolic compounds, lignin, and flavonoids; genes related to stress and defense responses; cell wall modification; cellular signaling; and transcriptional regulation. Among 7,431 SCN transcripts monitored, 1,850 genes showed statistically significant differential …

Sequence Mining And Transcript Profiling To Explore Cyst Nematode Parasitism, Axel A. Elling, Makedonka Mitreva, Xiaowu Gai, John Martin, Justin Recknor, Eric L. Davis, Richard S. Hussey, Dan Nettleton, James P. Mccarter, Thomas J. Baum

Dan Nettleton

Background: Cyst nematodes are devastating plant parasites that become sedentary within plant roots and induce the transformation of normal plant cells into elaborate feeding cells with the help of secreted effectors, the parasitism proteins. These proteins are the translation products of parasitism genes and are secreted molecular tools that allow cyst nematodes to infect plants.

Results: We present here the expression patterns of all previously described parasitism genes of the soybean cyst nematode, Heterodera glycines, in all major life stages except the adult male. These insights were gained by analyzing our gene expression dataset from experiments using the Affymetrix Soybean …

Flor Revisited (Again): Eqtl And Mutational Analysis Of Nb-Lrr Mediated Immunity To Powdery Mildew In Barley, Roger P. Wise, Priyanka Surana, Gregory S. Fuerst, Ruo Xu, Divya Mistry, Julie A. Dickerson, Dan Nettleton Dr.

Dan Nettleton

Genes encoding early signaling events in pathogen defense often are identified only by their phenotype. Such genes involved in barley-powdery mildew interactions include Mla, specifying race-specific resistance; Rar1 (Required for Mla12-specified resistance1), and Rom1 (Restoration of Mla-specified resistance1). The HSP90-SGT1-RAR1 complex appears to function as chaperone in MLA-specified resistance, however, much remains to be discovered regarding the precise signaling underlying plant immunity. Genetic analyses of fast-neutron mutants derived from CI 16151 (Mla6) uncovered a novel locus, designated Rar3 (Required for Mla6-specified resistance3). Rar3 segregates independent of Mla6 and Rar1, and rar3 mutants are susceptible to Blumeria graminis f. sp. hordei …

Biphasic Gene Expression Changes Elicited By Phakopsora Pachyrhizi In Soybean Correlate With Fungal Penetration And Haustoria Formation, Katherine T. Schneider, Martijn Van De Mortel, Timothy J. Bancroft, Edward J. Braun, Dan Nettleton, Rex T. Nelson, Reid D. Frederick, Thomas J. Baum, Michelle Graham, Steven A. Whitham

Dan Nettleton

Inoculation of soybean (Glycine max) plants with Phakopsora pachyrhizi, the causal organism of Asian soybean rust, elicits a biphasic response characterized by a burst of differential gene expression in the first 12 h. A quiescent period occurs from 24 to 48 h after inoculation, in which P. pachyrhizi continues to develop but does not elicit strong host responses, followed by a second phase of intense gene expression. To correlate soybean responses with P. pachyrhizi growth and development, we inoculated the soybean cultivar Ankur (accession PI462312), which carries the Rpp3 resistance gene, with avirulent and virulent isolates …

Distinct Biphasic Mrna Changes In Response To Asian Soybean Rust Infection, Martijn Van De Mortel, Justin C. Recknor, Michelle Graham, Dan Nettleton, Jaime D. Dittman, Rex T. Nelson, Claudia V. Godoy, Ricardo V. Abdelnoor, Alvaro M.R. Almeida, Thomas J. Baum, Steven A. Whitham

Dan Nettleton

Asian soybean rust (ASR), caused by Phakopsora pachyrhizi, is now established in all major soybean-producing countries. Currently, there is little information about the molecular basis of ASR–soybean interactions, which will be needed to assist future efforts to develop effective resistance. Toward this end, abundance changes of soybean mRNAs were measured over a 7-day ASR infection time course in mock-inoculated and infected leaves of a soybean accession (PI230970) carrying the Rpp2 resistance gene and a susceptible genotype (Embrapa-48). The expression profiles of differentially expressed genes (ASR-infected compared with the mock-inoculated control) revealed a biphasic response to ASR in each genotype. …

Developmental Transcript Profiling Of Cyst Nematode Feeding Cells In Soybean Roots, Nagabhushana Ithal, Justin Recknor, Dan Nettleton, Tom Maier, Thomas J. Baum, Melissa G. Mitchum

Dan Nettleton

Cyst nematodes of the genus Heterodera are obligate, sedentary endoparasites that have developed highly evolved relationships with specific host plant species. Successful parasitism involves significant physiological and morphological changes to plant root cells for the formation of specialized feeding cells called syncytia. To better understand the molecular mechanisms that lead to the development of nematode feeding cells, transcript profiling was conducted on developing syncytia induced by the soybean cyst nematode Heterodera glycines in soybean roots by coupling laser capture microdissection with high-density oligonucleotide microarray analysis. This approach has identified pathways that may play intrinsic roles in syncytium induction, formation, and …

Comparison Of Transcript Profiles In Wild-Type And O2 Maize Endosperm In Different Genetic Backgrounds, Hongwu Jia, Dan Nettleton, Joan M. Peterson, Gricelda Vasquez-Carrillo, Jean-Luc Jannink, M. Paul Scott

Dan Nettleton

Mutations in the Opaque2 (O2) gene of maize (Zea mays L.) improve the nutritional value of maize by reducing the level of zeins in the kernel. The phenotype of o2 grain is controlled by many modifier genes and is therefore strongly dependent on genetic background. We propose two hypotheses to explain differences in phenotypic severity in different genetic backgrounds: (i) Specific genes are differentially (o2 vs. wild-type) expressed only in certain genotypes, and (ii) A set of genes are differentially expressed in all backgrounds, but the degree of differential expression differs in different backgrounds. To determine …

A Diallel Analysis Of A Maize Donor Population Response To In Vivo Maternal Haploid Induction I: Inducibility, Gerald N. De La Fuente, Ursula K. Frei, Benjamin Trampe, Daniel Nettleton, Wei Zhang, Thomas Lubberstedt

Dan Nettleton

The maize in vivo maternal doubled haploid (DH) system is an important tool used by maize breeders and geneticists around the world. The ability to rapidly produce DH lines of maize for breeding allows breeders to quickly respond to new selection criteria based on the ever changing biotic and abiotic stresses that maize is subjected to across its growing area. There are two important steps in the generation of DH lines using the in vivo maternal DH system: 1) the production and identification of haploid progeny, and 2) the doubling of genomes to create fertile, diploid inbred lines that can …

Using Drones For Precision Agriculture, Jiyul Chang, Madhav P. Nepal

Madhav Nepal

In this teaching module, students will learn what Precision Agriculture is and how to apply drone into Precision Agriculture practices. To use data (images) taken by drone, students will learn the basic theory of Remote Sensing. Using images, students learn how to make NDVI (Normalized Difference Vegetation Index) maps and how to apply drone (remote sensing technique) in agriculture.

Medicago Sativa Has Reduced Biomass And Nodulation When Grown With Soil Microbiomes Conditioned To High Phosphorus Inputs, Laura M. Kaminsky, Grant L. Thompson, Ryan V. Trexler, Terrence H. Bell, Jenny Kao-Kniffin

Grant Thompson

Applying Biodiversity And Ecosystem Function Theory To Turfgrass Management, Grant L. Thompson, Jenny Kao-Kniffin

Grant Thompson

Impact Of Nitrogen Application Timing On Corn Production, John E. Sawyer, Daniel W. Barker, John P. Lundvall

John E. Sawyer

Water quality issues have renewed interest in timing of nitrogen (N) application as a means to improve use efficiency in corn and reduce losses. Improved economic return is also desired as N fertilization is one of the most costly inputs to corn production. Time of fertilizer application is a component of the site-specific 4R nutrient management stewardship programs. In Iowa, the Nutrient Reduction Strategy has a 7% (37% std. dev.) nitrate-N reduction with a 0% (3% std. dev.) corn yield change for sidedress compared to pre-plant N application (SP 0435A).

Crop Responses To Amisorb In The North Central Region, Sylvie Brouder, Robert Dowdy, Dave Franzen, Gary Hergert, Robert Hoeft, Maurice Horton, Keith Kelling, David Mengel, George Rehm, John Sawyer, Peter Scharf, Darryl Warnacke, David Whitney

John E. Sawyer

Originally used to prevent scale in boilers, carpramid or thermal polyaspartate (copoly[(3-carboxypropionamide)( 2-carboxylmethyl) acetamide)] was brought to agriculture under the trade names AmiSorb and Magnet. It claimed to increase nutrient uptake through artificially increasing the volume of soil occupied by roots through increased root branching and root hair development. Under controlled hydroponic or greenhouse conditions, the use of carpramid increased nutrient uptake, some yield determining factors such as wheat tillering and in some cases, crop yield. Extensive field testing from 1996 to 1998 under various nutrient regimes, placements, forms, and timings resulted in very inconsistent performance. Averaged across all experiments …

Nitrogen Fertilizer Rate Effects On Soil Organic Carbon In Iowa Continuous Corn And Corn-Soybean Systems, Hanna J. Poffenbarger, Daniel W. Barker, Matthew J. Helmers, Fernando E. Miguez, John E. Sawyer, Johan Six, Michael J. Castellano

John E. Sawyer

Nitrogen fertilizer rate is a key factor affecting soil organic C (SOC) in corn-based cropping systems. The objective of this study was to determine the change in SOC in response to long-term N rates for continuous corn and corn-soybean cropping systems at two sites in Iowa. Soil samples were collected to a depth of 15 cm in 1999 and again in 2014 after 15 years of corn N rate treatments ranging from 0 to 269 kg ha^-1. The soil samples were analyzed for total C and N concentrations. For continuous corn at both sites, the average annual change …

Winter Grazing Management, Stephen K. Barnhart, James R. Russell, Douglas L. Karlen, Michael J. Tidman

Douglas L Karlen

Why winter grazing? Beef cow herd and sheep flock records show that winter feeding costs are livestock producers' single largest production expense. Managing through winter weather while keeping feeding costs low is an essential part of maintaining a profitable operation. Iowa's climate generally allows forage growth only during a 7-to-8 month period. Extending the grazing of this forage--even an extra 3 or 4 weeks in late autumn and winter--is an economical way to maintain or increase livestock profitability. Some producers extend the grazing season by using stockpiled forage, whereas others use crop residue, and many combine the use of stockpiled …

Engineering, Nutrient Removal, And Feedstock Conversion Evaluations Of Four Corn Stover Harvest Scenarios, Reed L. Hoskinson, Douglas L. Karlen, Stuart J. Birrell, Corey W. Radtke, Wally Wilhelm

Douglas L Karlen

Crop residue has been identified as a near-term source of biomass for renewable fuel, heat, power, chemicals and other bio-materials. A prototype one-pass harvest system was used to collect residue samples from a corn (Zea mays L.) field near Ames, IA. Four harvest scenarios (low cut, high-cut top, high-cut bottom, and normal cut) were evaluated and are expressed as collected stover harvest indices (CSHI). High-cut top and high-cut bottom samples were obtained from the same plot in separate operations. Chemical composition, dilute acid pretreatment response, ethanol conversion yield and efficiency, and thermochemical conversion for each scenario were determined. Mean …

Protocol For Indicator Scoring In The Soil Management Assessment Framework (Smaf), Brian J. Wienhold, D.L. Karlen, S.S. Andrews, D.E. Stott

Douglas L Karlen

Assessment tools are needed to evaluate agronomic management effects on critical soil functions such as carbon sequestration, nutrient cycling and water partitioning. These tools need to be flexible in terms of selection of soil functions to be assessed and indicators to be measured to ensure that assessments are appropriate for the management goals. The soil management assessment framework (SMAF) is being developed to meet this need. The SMAF uses soil physical, chemical and biological indicator data to assess management effects on soil function using a three-step process for (1) indicator selection, (2) indicator interpretation and (3) integration into an index. …

Review And Interpretation: Nitrogen Management Strategies To Reduce Nitrate Leaching In Tile-Drained Midwestern Soils, Dana L. Dinnes, Douglas L. Karlen, Dan B. Jaynes, Thomas C. Kaspar, Jerry L. Hatfield, Thomas S. Colvin, Cynthia A. Cambardella

Douglas L Karlen

Balancing the amount of N needed for optimum plant growth while minimizing the NO3 that is transported to ground and surface waters remains a major challenge for everyone attempting to understand and improve agricultural nutrient use efficiency. Our objectives for this review are to examine how changes in agricultural management practices during the past century have affected N in Midwestern soils and to identify the types of research and management practices needed to reduce the potential for nonpoint NO3 leakage into water resources. Inherent soil characteristics and management practices contributing to nonpoint NO3 loss from Midwestern soils, the impact of …

Fluid Fertilizer’S Role In Sustaining Soils Used For Bio-Fuels Production, John L. Kovar, Douglas Karlen

Douglas L Karlen

The short- and long-term effects on soil nutrient cycling, physical properties, and biological activity of striving for higher grain yields and removing crop residues for bio-fuels production must be understood to provide more quantitative crop and soil management guidelines. This study focuses on potassium (K) and sulfur (S) requirements of corn (Zea mays L.) grown for bioenergy feedstock production. Our objectives for 2008 were to: i) evaluate the performance of several S fertilizers, including liquid ammonium thiosulfate (12-0-0-26S), as S sources for corn grown in Iowa, and ii) establish a comprehensive tillage, nutrient management, crop residue removal, and cover crop …

Winter Rye Cover Crop Biomass Production, Degradation, And N Recycling, J. L. Pantoja, J. E. Sawyer, D. W. Barker

John E. Sawyer

Winter rye (Secale cereale L.) as a cover crop can take up residual inorganic N between annual row crops and therefore be used to help reduce NO3 -–N loss from fields and movement to water systems. However, does the rye N uptake affect N recycling to soil and add to plant available N? The rye carbon:nitrogen (C:N) ratio could also influence N recycling. The objectives of this study were to evaluate rye biomass degradation and N recycling after spring rye termination in a no-till corn (Zea mays L.) - soybean [Glycine max. (L.) Merr.] rotation. A two year experiment (2010-2011) …

In-Season Nitrogen Fertilization Of Soybean, J. E. Sawyer, D. W. Barker

John E. Sawyer

Nitrogen (N) fertilization is not a traditional nutrient management practice for soybean production in Iowa. Soybean is a legume plant and is assumed to adequately obtain needed N through symbiotic fixation. However, there has been interest over the years in using N fertilization to increase yield and grain protein due to the recognition of the large N requirement associated with high yields. Depending on the soil residual inorganic N level and soil N mineralization characteristics, approximately 40 to 75% of the N in a mature soybean plant is derived from the soil (Shibles, 1998). Also, soybean seems to require this …

Corn And Soybean Response To Sulfur Application On Iowa Soils, J. E. Sawyer, D. W. Barker

John E. Sawyer

Historically sulfur (S) application has not been recommended on Iowa soils for com and soybean production. Prior research has not determined a consistent need for S fertilization in Iowa, with field research indicating no com or soybean yield response to applied S at virtually every site studied (Thorup and Leitch 1975; Webb, 1978; Alesii 1982; Killorn, 1984; Sexton et al., 1998; Mallarino et al., 2000). The soil supply, in combination with sources such as manure and atmospheric deposition has apparently met com and soybean S needs. Sulfur deficiencies have been reported over the years in various areas of the Midwestern …