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Microfluidic Interfaces For Coupling Hydrodynamic To Electrokinetic Flow And Microchannel Electrophoresis To Electrospray Ionization-Mass Spectrometry, Brent R. Reschke
Microfluidic Interfaces For Coupling Hydrodynamic To Electrokinetic Flow And Microchannel Electrophoresis To Electrospray Ionization-Mass Spectrometry, Brent R. Reschke
Graduate Theses, Dissertations, and Problem Reports
Presented in this dissertation is a microfluidic interface for coupling hydrodynamic and electrokinetic flows, this interface was first studied theoretically and then experimentally to evaluate the electrophoretic extraction of a negatively charged analyte from a perpendicular hydrodynamic flow stream. Secondly the interface was used to perform the simultaneous extraction and separation of a cationic peptide and anionic proteins with low electroosmotic flow.;In addition, two important aspects of creating a microfluidic device to couple microchannel electrophoresis with mass spectrometry using electrospray ionization were also studied. These aspects included the electrospray emitter from which the electrospray originates and the incorporation of the …