Digital Commons Network^™

Evaluation Of Genomic Predictors For Red Angus Cattle, Stephen D. Kachman, Matthew L. Spangler

Nebraska Beef Cattle Reports

Purebred Red Angus genotypes, via the Ilumina BovineSNP50 assay, and expected progeny differences (EPD) were used to evaluate the accuracy of genomic predictors for traits that are currently reportedthrough the American Red AngusAssociations’ National Cattle Evaluation. Two genomic predictors were evaluated, one derived using prediction equations from the National Beef Cattle Evaluation Consortium and the other from Zoetis.

Effect Of Distillers Grains Plus Solubles Supplementation On Grazing Cattle Performance, Tyler L. Hasenauer, Terry J. Klopfenstein, James C. Macdonald Donald, Cody J. Schneider, Dirk B. Burken

Nebraska Beef Cattle Reports

Yearlings rotationally grazing smooth bromegrass were individually supplemented modified distillers grains plus solubles (MDGS) at .05, 0.4, 0.6, and 0.8% BW. Gain increased quadratically as MDGS level increased. Maximal ADG (2.95 lb/d) was predicted when supplementing level of 0.48% of BW. Economic analysis compared 0, 2, and 5 lb (DM) MDGS supplementation. When cattle ownership was retained through the feeding period, MDGS supplementation was profitable. Supplementation at 2 lb (DM) was more profitable than 5 lb (DM) when MDGS is above $265.63/ton (DM) or 85% the price of $7.50 /bu corn.

Replacement Of Grazed Forage And Animal Performance When Distillers Grains Are Fed In A Bunk Or On The Ground On Summer Range, Kari L. Gillespie, L. Aaron Stalker, Terry J. Klopfenstein Klopfenstein, Jerry D. Volesky, Jacki A. Musgrave

Nebraska Beef Cattle Reports

Forage savings and supplement loss caused by feeding on the ground were estimated when spayed yearling heiferswere fed modified distillers grains with solubles (MDGS) while grazing Sandhills summer range. Across two years, heifers fed 0.6% BW MDGS had 1.39 lb greater ADG and consumed approximately 17% less forage than non-supplemented heifers. Calculated loss of MDGS when fed on the ground was 5.6%. Supplementing MDGS decreased forage consumption approximately 17% and increased summer gains.

Effects Of Forage Quality, Mdgs, And Monensin On Performance, Methane Concentration, And Ruminal Fermentation Of Growing Cattle, Anna C. Pesta, Andrea K. Watson, Samodha Fernando Fernando, Galen E. Erickson

Nebraska Beef Cattle Reports

A growing study was conducted to evaluate a novel method for measuring methane concentration by feedlot cattle, and to determine the effects of forage quality, inclusion of modified distillers grains plus solubles (MDGS), and presence or absence of monensin on performance, methane concentration, and rumen fermentation characteristics. Performance was improved by use of high-quality forage and MDGS, while response to monensin was variable across basal diet type. Response of methane concentration and volatile fatty acid (VFA) profile due to diet was variable and subject to multiple interactions, reflecting the complexity of the microbial processes involved within the rumen.

Effects Of Increasing Inclusion Of Wet Distillers Grains Plus Solubles With And Without Oil Extraction On Finishing Performance, Melissa L. Jolly, Brandon L. Nuttelman, Dirk Burken Burken, Cody J. Schneider, Terry J. Klopfenstein, Galen E. Erickson

Nebraska Beef Cattle Reports

A finishing study was conducted to assess the effects of feeding increasing amounts of wet distillers grains plus solubles (WDGS) with and without corn oil removal. Oil removal and WDGS inclusion did not interact. Compared to normal oil, de-oiled WDGS did not impact ADG, F:G, or carcass characteristics. Increasing inclusion of WDGS decreasedDMI and F:G linearly, with no change in ADG. Regardless of inclusion, oil removal via centrifugation had little impact on finishing cattle performance.

Effect Of Feeding Different Types Of Byproducts And Concentrations Throughout A Beef Growing System On Ground Beef Color And Lipid Oxidation, Joe O. Buntyn, Brandy D. Cleveland, Amy L. Redfield Redfield, James C. Macdonald, Galen E. Erickson, Tommi F. Jones, Ty B. Schmidt, Gary A. Sullivan

Nebraska Beef Cattle Reports

The objective of this trial was to evaluate the effect of feeding different concentrations of wet distillers grains during winter backgrounding and either modified wet distillers grains or Sweet Bran^® during the finishing phase on ground beef color and lipid oxidation. After a 14 day aging period, ground beef patties were made and placed in a simulated retail display for seven days. There were no overall differences in lipid oxidation between treatments but was a treatment by day interaction for discoloration. Ground beef from heifers finished with modified wet distillers grains discolored at a greater extent when compared to …

The Current Prevalence Of Caprine Arthritis-Encephalitis Virus In Midwestern Goat Herds, Bradley Todd Jones Dvm

School of Veterinary and Biomedical Sciences: Dissertations, Theses, and Student Research

Caprine Arthritis-Encephalitis Virus (CAEV) is an incurable disease of goats that has both social and economic impacts. Clinical disease in goats includes encephalitis in kids; chronic arthritis, inflammatory mastitis and progressive respiratory disease in adults. In the last 25 years there have been significant changes in the US goat industry with rapid growth in the meat and dairy industries. Recent prevalence studies are lacking and historic studies may not reflect changes in the industry. The purpose of this study was to establish the prevalence of CAEV in Midwestern herds that are not routinely acquiring new animals from known negative CAEV …

Androgen Excess In Beef Cows Results In Altered Theca Cell Gene Expression And Fertilityand, Adam F. Summers, William E. Pohlmeier, Vanessa M. Brauer Brauer, Kevin M. Sargent, Renee M. Mcfee, Scott G. Kurz, Robert A. Cushman, Jennifer R. Wood, Andrea S. Cupp

Nebraska Beef Cattle Reports

Within the University of Nebraska–Lincoln physiology herd, two sub-populations of cows with different concentrations of androstenedione have been identified. Androstenedione is a precursor for estradiol production, and androstenedione concentration is increased 24.5-fold in the high androstenedione cows. Our objective was to determine the cause of increased androstenedione production in high androstenedione cows and the effects on theca cell and oocyte gene expression. High androstenedione cows had increased steroidogenic enzyme abundance in theca cells and altered oocyte mRNA abundance. Increased androgen production in high androstenedione cows is associated with altered gene expression and/or mRNA stability during oocyte growth and maturation, which …

Comparison Of Long-Term Progestin-Based Synchronization Protocols On Fixed-Time Ai Pregnancy Rate In Beef Heifers, Rebecca A. Vraspir, Adam F. Summers, Doug O'Hare Hare, Larry D. Rowden, Rick N. Funston

Nebraska Beef Cattle Reports

Yearling Angus heifers at a commercial ranch in the Nebraska Sandhills were randomly assigned to one of two progestin-based fixed-time AI protocols (MGA or 14-day CIDR) to compare pregnancy rates. Heifers had similar fixed-time AI pregnancy rates between MGA and 14-day CIDR. A similar proportion of MGA and 14-day CIDR heifers displayed a second estrus; however, heifers previously synchronized with MGA tended to have a greater second AI pregnancy rate. Overall pregnancy rate was similar between MGA and 14-day CIDR treatments. The MGA system was the more cost effective synchronization protocol in this study.

Distillers Grains Supplementation In A Forage System With Spayed Heifers, Kari L. Gillespie, Terry J. Klopfenstein, James C. Macdonald Donald, Brandon L. Nuttelman, Cody J. Schneider, Jerry D. Volesky, Galen Erickson

Nebraska Beef Cattle Reports

Spayed heifers were developed into yearlings by grazing corn residue and bromegrass, followed by native range, and were finished on a common diet. Treatments were 2 lb or 5 lb of wet distillers grains with solubles (WDGS; DM basis) supplement on corn residue daily, and modified distillers grains with solubles (MDGS) fed at 0.6% BW daily or no MDGS during summer grazing. Feeding 5 lb increased winter ADG by 0.68 lb (year 1) or 0.40 lb (year 2) compared to 2 lb, and increased HCW after finishing. Summer supplementation increased summer ADG by 0.50 lb (year 1) or 0. 44 …

Alkaline Treated Wheat Straw Or Corn Stover Fed To Growing Calves, Adam L. Shreck, Brandon L. Nuttelman, Cody J. Schneider Schneider, Dirk B. Burken, Casey N. Macken, William A. Griffin, Galen E. Erickson, Terry J. Klopfenstein

Nebraska Beef Cattle Reports

Four hundred sixty steer calves were fed CaO treated (5% of DM) or untreated wheat straw and corn stover with wet distillers grains plus solubles (WDGS) during a 69-day growing study. An interaction between crop residue and alkaline treatment was observed for ending BW and ADG. The relative response in ADG and ending BW due to alkaline treatment was greater for wheat straw compared to corn stover. Steers fed wheat straw diets had greater DMI and improved F:G compared to corn stover diets. Alkaline treatment increased DMI and improved F:G, although the F:G response was small. Growing calves on untreated …

Effects Of Grazing On Nebraska Sandhills Meadow Forage Nutrient Content, Jared V. Judy, Jacki A. Musgrave, L. Aaron Stalker Stalker, Karla H. Jenkins, Terry J. Klopfenstein

Nebraska Beef Cattle Reports

Nebraska Sandhills subirrigated meadow pastures were utilized to measure the effects of grazing on forage nutrient content in summer pastures. Pre-grazed pastures had greater protein and in vitro dry matter digestibility levelsand lower neutral detergent fiber levels compared with post-grazed pastures early in the grazing season. By late July, post-grazed vs. pre-grazed pastures did not differ in in vitro dry matter digestibility and neutral detergent fiber levels. Observed results indicate the greatest differences in nutrient content between post-grazed and pre-grazed pastures occur early in the grazing season.

Effect Of Distillers Grains Supplementation On Calves Grazing Irrigated Or Non-Irrigated Corn Residue, Mandi Jones, James C. Macdonald, Galen Erickson Erickson, Terry J. Klopfenstein, Andrea K. Watson

Nebraska Beef Cattle Reports

Steer calves grazing irrigated or non-irrigated corn residue received supplementation of dried or modified distillers grains plus solubles (DGS) at 0.3, 0.7, or 1.1% of BW . Steers were individually supplemented daily through Calan gates. Daily gain improved quadratically with increasing supplementation (1.55 lb/day to 2.12 lb/day) and for calves grazing non-irrigated (2.02 lb/day) compared to irrigated (1.77 lb/day) corn residue. Feeding dry instead of modified DGS did not significantly impact ADG. Supplementing DGS to calves grazing corn residue increased gain during the winter period.

Nutrient And Tenderness Differences Of Beef From Heifers Due To Mutation Of The Myostatin Genehypothesized, Michelle E. Semler, Chris R. Calkins, Galen E. Erickson Erickson

Nebraska Beef Cattle Reports

Strip loins and eye of rounds were obtained from heifers genotyped with variations of the myostatin gene; 19 homozygous dominant (Angus), 20 heterozygous dominant (Angus x Piedmontese), and 20 homozygous recessive (Piedmontese). Steaks were aged for 14 days, cooked fresh (never frozen), and nutrient steaks were frozen three days postmortem. Meat from homozygous recessive heifers was equal in tenderness to homozygous dominant and heterozygous dominant heifers. Fat content of meat from homozygous recessive heifers decreased while moisture and protein increased compared to homozygous dominant and heterozygous dominant. Calorie content decreased with increasing copies of the recessive gene. Thus, meat from …

Lipid Oxidation In Cooked Ground Beef Links From Cattle Fed Distillers Grains In Different Phases Of Production, Brandy D. Cleveland, Joe O. Buntyn, Amy L. Redfield Redfield, James C. Macdonald, Galen E. Erickson, Tommi F. Jones, Ty B. Schmidt, Gary A. Sullivan

Nebraska Beef Cattle Reports

Ground beef links from cattle fed high or low levels of distillers grains during backgrounding and Sweet Bran^® or modified wet distillers grains in finishing diets were compared to analyze oxidation over time. Ready-to-eat beef links from cattle fed 5 lb/head/day (DM basis) of wet distillers grains during backgrounding had greater oxidative rancidity with extended storage than those from cattle fed 2 lb/head/day (DM basis). Beef links from cattle finished with wet distillers grains oxidized more rapidly than those fed Sweet Bran. Therefore, cooked beef from cattle fed distillers grains during either phase of production (backgrounding or finishing) showed …

Differences In Fecal Bacterial Community Composition Between Beef Steers Which Are High-Shedders And Low-Shedders Of Shiga Toxin-Producing Escherichia Coli (Stec), Nirosh D. Aluthge, Yoshitha A. Wanniarachchi, Brandon L. Nuttelman Nuttelman, Cody J. Schneider, Terry J. Klopfenstein, Galen E. Erickson, Samodha C. Fernando

Nebraska Beef Cattle Reports

The community composition of the fecal microbiota was compared between beef steers which were high-shedders and low-shedders of Shiga toxin-producing Escherichia coli. Based on Shannon and Chao 1 diversity indices, the high-shedders had a more diverse fecal bacterial community than the low-shedding steers. Members of the genus Prevotella were observed as being more abundant in the low-shedders compared to the high-shedders, while Succinivibrio were more abundant in the high-shedders. Isolation of specific bacteria which are significantly more abundant in low-shedders may pave the way to developing direct-fed microbials which are effective in reducing STEC shedding among high-shedding beef steers.

The Effects Of Diet And Cooler Aging On Specific Flavor Notes In Beef, Kimberly A. Varnold, Chris R. Calkins, Rhonda K. Miller Miller, Galen E. Erickson

Nebraska Beef Cattle Reports

Crossbred steers (n = 64) were grazed on warm- or cool-season grass-dominated pastures, without or with energy supplementation of wet distillers grains with solubles (WDGS), and were finished on a corn-based diet with or without 35% WDGS. Finishing on corn increased desirable flavor notes and decreasedundesirable flavor notes in both L. dorsi and B. femoris steaks. In addition, grazing on warm-season grasses increased the prevalence of undesirable flavors but was often dissipated by the addition WDGS supplementation. Longer aging periods tended to increase the prevalence of undesirable flavors, especially in B. femoris steaks. It is recommended producers provide WDGS supplementation, …

Vein Steak Differences In Strip Loins Of Heifers Due To Mutation Of The Myostatin Gene, Michelle E. Semler, Chris R. Calkins, Galen E. Erickson Erickson

Nebraska Beef Cattle Reports

Strip loins from heifers with variations of the myostatin gene; 19 homozygous dominant (Angus), 20 heterozygous dominant (Angus x Piedmontese), and 20 homozygous recessive (Piedmontese) were studied. Strip loins were cut into 1-inch thick steaks and total number of steaks and number of steaks with Gluteusmedius (often called vein steaks) were recorded. Strip loins from heterozygous dominant heifershad a greater number of non-vein steaks and decreased percentage of vein steaks compared to homozygous dominant and homozygous recessive samples. Differences in percentage of vein steaks were inconsistent and showed no meaningful pattern.

Dryland Cover Crops As A Grazing Option For Beef Cattle, Alex H. Titlow, Jake A. Hansen, Matt K. Luebbe Luebbe, Terry J. Klopfenstein, Karla H. Jenkins

Nebraska Beef Cattle Reports

A two-year grazing study was conducted to evaluate forage quality and utilization of cover crops (CC) in dryland cropping systems compared to crested wheatgrass pastures (CWP). The CC mixture consisted of oats, peas and turnips planted in March with a no-till drill. Both CC and CWP were grazed during the month of June. Total tract dry matter digestibility and CP were greater for CC compared to CWP while NDF and ADF of CC were less. The CC was observed to have greater forage quality over both years and may produce similar amounts of forage as crested wheatgrass pastures allowing deferred …

Optimal Marketing Date Of Steers Depends On Marketing Strategy, James C. Macdonald, Cody J. Schneider, Kelsey M. Rolfe Rolfe, Stephen D. Kachman, Terry J. Klopfenstein, Galen E. Erickson

Nebraska Beef Cattle Reports

Seven research trials conducted over five years at the University of Nebraska–Lincoln were summarized to determine how animal performance changes through the finishing period on a live and carcass weight basis. Live weight, carcass weight, carcass ADG, and carcass feed efficiency all changed quadratically throughout the feeding period; live ADG and live feed efficiency declined linearly. During times of negative profit margins, optimal profitability for steers marketed on a live-basis occurred by selling early, whereas optimal profitability was achieved by feeding steers marketed on a carcass-basis longer.

Longevity And Mortality Of Owned Dogs In England, D. G. O'Neill, D. B. Church, P. D. Mcgreevy, P. C. Thomson, D. C. Brodbelt

Epidemiology Collection

Improved understanding of longevity represents a significant welfare opportunity for the domestic dog, given its unparalleled morphological diversity. Epidemiological research using electronic patient records (EPRs) collected from primary veterinary practices overcomes many inherent limitations of referral clinic, owner questionnaire and pet insurance data. Clinical health data from 102,609 owned dogs attending first opinion veterinary practices (n = 86) in central and southeast England were analysed, focusing on 5095 confirmed deaths.

Of deceased dogs with information available, 3961 (77.9%) were purebred, 2386 (47.0%) were female, 2528 (49.8%) were neutered and 1105 (21.7%) were insured. The overall median longevity was 12.0 years …

Canine Chronic Kidney Disease In Uk Veterinary Practices: Prevalence, Risk Factors And Survival, D. G. O'Neill, J. Elliott, D. B. Church, P. D. Mcgreevy, P. C. Thomson, D. C. Brodbelt

Epidemiology Collection

Analyses covering 107,214 dogs attending 89 UK practices aimed to estimate the prevalence and identify risk factors associated with canine chronic kidney disease (CKD) diagnosis and survival. The apparent prevalence (AP) was estimated using a cross-sectional approach while the true prevalence (TP) was estimated using Bayesian analysis. A nested case-control study design was used to evaluate risk factors. Survival analysis was conducted using the Kaplan-Meier survival curve method and multivariable Cox proportional hazards regression modelling. CKD AP was estimated at 0.21% and TP at 0.37%. CKD risk factors included increasing age, being insured and certain breeds. The median survival time …

Hormonal Residues In Feedlot Pens And Runoff, Terry L. Mader, Leslie J. Johnson, Shannon L. Bartelt-Hunt Hunt, Daniel D. Snow, William L. Kranz, Charles A. Shapiro, Simon Van Donk, David P. Shelton, David Tarkalson, Tian Zhang

Nebraska Beef Cattle Reports

Two identically designed trials were conducted in separate years at the University of Nebraska Haskell Agricultural Laboratory, Concord, Neb., using 192 crossbred heifers (96/trial). Within a trial, heifers were assigned randomly to 2 groups (3 pens/group): 1) treatment (TRT) animals were administered synthetic hormones via subcutaneous implants (Ralgro and Revalor-H) and fed Melengesterol Acetate (MGA), or 2) control (CON) animals with no synthetic hormone provided.

Gains and feed conversions were 18.8 and 7.5% better, respectively, for TRT, while CON had 16.7% greater choice and prime carcasses. In runoff samples, progesterone was greater for CON. With the exception of androsterone, average …

Effect Of Development System On Heifer Performance And Primiparous Heifer Grazing Behavior, Adam F. Summers, T. L. Meyer, Jacqueline Musgrave Musgrave, Stetson P. Weber, Rick N. Funston

Nebraska Beef Cattle Reports

The effect of heifer development system on primiparous heifer performance grazing corn residue during late gestation was investigated. Weaned heifers grazing corn residue tended to have reduced final BW after corn residue grazing compared to heifers grazing winter range. However reproductive performance for the two treatments was similar. When grazing corn residue as pregnant heifers during late gestation, heifers developed on corn residue had improved ADG compared to drylot-developed heifers and tended to have increased ADG compared to winter range-developed heifers. Adaptation to corn residue grazing as a developing heifer improves primiparous heifer performance grazing corn residue during late gestation.

Economic Analysis Update: Supplementing Distillers Grains To Grazing Yearling Steers, Stephanie K. Moore, Andrea K. Watson, Terry Klopfenstein Klopfenstein, Galen E. Erickson, Walter H. Schacht

Nebraska Beef Cattle Reports

A seven-year study from 2005-2011 was conducted to evaluate four grazing management strategies for backgrounding yearling steers on smooth bromegrass pastures. Economic budgets were used to calculate profit differences with current (April 2012) market prices. Overall, cattle receiving supplement had greater net returns, lower cost of gain, and lower breakeven prices. In recent years fertilizer prices have increased at a greater rate than land costs in Nebraska, making it more economical to use a lower stocking rate instead of fertilizing pastures. As land prices increase, the incentive to use either N fertilizer or DDGS supplementation increases.

Evaluation Of A New Chemistry For Rangeland Grasshopper Control, Jeffrey Bradshaw, Karla Jenkins, Sean Doyle Whipple Whipple, Rick Patrick

Nebraska Beef Cattle Reports

A grasshopper control study was conducted to evaluate the effectiveness of a new class of systemic chemical. The new pesticide Prevathon^® (high and low levels) was compared to Coragen^®, Dimilin^®, and a nontreated check. Grasshoppers were numerically reduced the most by Coragen and Prevathon, though not significant. The highest level of Prevathon did not numerically impact beneficial insects in general. Biomass and forage quality were not significantly impacted by chemical treatment. However, forage biomass was numericallygreatest for the highest level of Prevathon. Prevathon appears to be an acceptable systemic pesticide for grasshopper control with minimal …

Replacing Steam-Flaked Corn And Dry Rolled Corn With Condensed Distillers Solubles In Finishing Diets, Alex H. Titlow, Adam L. Shreck, Stephanie A. Furman Furman, Karla Jenkins, Matt K. Luebbe, Galen E. Erickson

Nebraska Beef Cattle Reports

The interaction between corn processing method and condensed distillers solubles (CDS) was evaluated using either steam-flaked corn (SFC) or dry rolled corn (DRC). As CDS replaced corn at either 15 or 30% of the diet DM, DMI intake decreased quadratically for both SFC and DRC. Within DRC- based diets, ADG increased quadratically with 15% CDS being greatest and F:G improved quadratically with 30% inclusion being best. When SFC was used as the grain source, ADG increased linearly and F:G improved quadratically with increasing levels of CDS. A greater performance response was observed with SFC compared to DRC when feeding increased …

Including Next Enhance® Essential Oils In Finishing Diets On Performance With Or Without Rumensin® And Tylan®, Curtis J. Bittner, Galen E. Erickson, Karla Jenkins Jenkins, Matt K. Luebbe, Troy Wistuba

Nebraska Beef Cattle Reports

Finishing cattle performance was evaluated using NEXT ENHANCE essential oils in finishing diets. Treatments consisted of 1) control (CON), 2) NEXT ENHANCE at 300 mg/day (NE), 3) Rumensin and Tylan at 360 and 90 mg/day, respectively (RT), or 4) NEXT ENHANCE plus Rumensin and Tylan (NERT). No NEXT ENHANCE by Rumensin/Tylan interaction was observed. Steers fed Rumensin/Tylan had decreased F:G and increased live final BW and marbling score. The prevalence of liver abscesses decreased 46% when steers were fed Rumensin/Tylan. Including NEXT ENHANCE in finishing diets did not impact performance or carcass characteristics.

The Effect Of Commensal Microbial Communities On The Fecal Shedding Of Shiga Toxin-Producing E. Coli (Stec) In Beef Cattle, Nirosh D. Aluthge, Yoshitha A. Wanniarachchi, Brandon L. Nuttelman Nuttelman, Cody J. Schneider, Terry Klopfenstein, Galen E. Erickson, Jayne E. Stratton, Samodha C. Fernando

Nebraska Beef Cattle Reports

This ongoing study compares the gut microbial community composition between shedding steers high in shiga toxin-producing E. coli (STEC) counts and low-shedding steers. Shedders were identified among 170 beef animals over three time periods using selective microbiological culture media. The isolated bacterial cultures were confirmed to be STEC using PCR, 16s rRNA sequencing and a shiga toxin immunoassay. The most abundant strains found in the cattle feces were those belonging to the serogroups O111 (40.3%) and O157:H7 (37.3%), with O103 (8.3%), O26 (6.0%), O83 (4.5%), and O55 (3.0%) being detected in much lower numbers . Out of the 52 animals …

Nitrogen Fertilization And Ddgs Supplementation Reduces Annual Weeds In Pastures, John A. Guretzky, Laura K. Snell, John C. Soper Soper, Walter H. Schacht, Terry Klopfenstein, Stephanie K. Pruitt

Nebraska Beef Cattle Reports

Ongoing research has found body weight (BW) gains of steers supplemented with corn dried distillers grains plus solubles (DDGS) on unfertilized smooth bromegrass pasture (SUPP) to be greater than unsupplemented steers on N fertilized (FERT) and unfertilized, control (CONT) smooth bromegrass pasture. In the seventh year of the study, annual weeds increased to 20%, 9%, and 2% of relative cover within CONT, SUPP, and FERT pastures, respectively. Supplementation of DDGS on unfertilized pastures improves steer BW gains and reduces N inputs while providing intermediate resistance to annual weed invasion. AnnualN fertilization maximizes forage yield and minimizes annual weeds in pasture.