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Development Of A Crispr System To Mutate Lpar4 In Chick Retinal Ganglion Cells, Garrett Driscoll
Development Of A Crispr System To Mutate Lpar4 In Chick Retinal Ganglion Cells, Garrett Driscoll
Graduate Theses
Growth cones direct axon pathfinding during neural development by detecting environmental stimuli, known as axon guidance molecules. In vitro studies have shown inhibitory axon guidance molecules to cause growth cones to change morphology to what is called a collapsed growth cone. Lysophosphatidic acid (LPA) has been demonstrated to collapse growth cones in culture, and thus it may act as an inhibitory molecule. However, identification of what LPA receptor is responsible for this physiological response is unknown. To investigate which receptor elicits collapse, we focused on LPA receptor-4 (LPA4) by designing a CRISPR construct to mutate Lpar4 in chick retinal ganglion …