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Genetic Considerations For The Conservation Of The Red Junglefowl, Courtney Lee Mcpheron

University Honors Theses

The undomesticated wild populations of our domestic species deserve heightened consideration when it comes to their conservation. Not only do they carry significant socio-cultural and economic roles, but they offer an exceptionally unique case study for population genetics. The red junglefowl (Gallus gallus) of southern and south-eastern Asia is considered to be the living ancestor and progenitor of the domestic chicken. This literature review examines how published research has evaluated its genetic relationship with the chicken and implications for its conservation. A special emphasis is given to the threat of genetic introgression with domestic and feral chicken populations …

Intestinal Microbiota Analysis Of Broiler Chickens Under Necrotic Enteritis Challenge And Tributyrin Supplementation, Taylor Nicole Mckinney

Graduate Theses and Dissertations

Poultry is a staple protein source for most of the planet. Until recently, antibiotic growth promoters (AGPs) were used to prevent illnesses in commercial chicken production. Currently, this is not possible due to regulations and consumer concern, but without such a preventative, diseases like necrotic enteritis (NE) have reemerged, posing a threat to bird health, and ultimately, our food source. Necrotic enteritis is a severe gastrointestinal disease caused by the gram-positive pathogen, Clostridium perfringens. Clinical features of this disease are diarrhea, intestinal lesions, and death, with a high transmission rate. In a subclinical form, growth performance is diminished and is …

The Role Of Microbiota On Campylobacter Jejuni Colonization And Growth Performance In Broiler Chickens, Ayidh Almansour

Graduate Theses and Dissertations

The successful poultry production at modern era comes from the vertical integratedindustrialization, which has fundamentally changed how animals have been living for millions of years. Antimicrobial growth promoters have been used to sustain the efficient industrialized animal production, driving antimicrobial overuse and resistance. Because of the increasing pressure from consumer’s concerns and government regulations, it is urgent to develop antimicrobial free alternatives as growth promoters in poultry production, but few effective antimicrobial alternatives are currently available. Campylobacter jejuni is one of the worldwide prevalent foodborne bacterial pathogens mainly transmitted from poultry. However, few mechanisms are available on why C. jejuni …

“The Role Of Poultry On Food Insecurity In Mozambique”, Austin Heinisch

Finance Undergraduate Honors Theses

Hunger, poverty, and starvation in developing countries are some of the most prevalent challenges facing the world today but many people outside of these areas are not fully aware as to just how bad it is. In the country of Mozambique, where the following research took place, food insecurity levels are rampant, and many different groups/companies are trying to change that. Through a service-learning study abroad program in the summer of 2019, my group and I worked with the local company New Horizons to research ways in which poultry production for both the company and their rural out growers could …

Development Of A Crispr System To Mutate Lpar4 In Chick Retinal Ganglion Cells, Garrett Driscoll

Graduate Theses

Growth cones direct axon pathfinding during neural development by detecting environmental stimuli, known as axon guidance molecules. In vitro studies have shown inhibitory axon guidance molecules to cause growth cones to change morphology to what is called a collapsed growth cone. Lysophosphatidic acid (LPA) has been demonstrated to collapse growth cones in culture, and thus it may act as an inhibitory molecule. However, identification of what LPA receptor is responsible for this physiological response is unknown. To investigate which receptor elicits collapse, we focused on LPA receptor-4 (LPA4) by designing a CRISPR construct to mutate Lpar4 in chick retinal ganglion …

Mechanistic Understanding Of Leaky Gut Syndrome In Heat Stressed Broiler Chickens, Jennifer Hoover

Animal Science Undergraduate Honors Theses

Abstract

Broilers, meat-type chickens, are bred for high growth rate and meat yield. These chickens have specific challenges due to heat stress. The adverse effects that impact these chickens have lead us to study the mechanisms involved. The objective of this study is to evaluate the expression of genes involved in intestinal permeability of heat- stressed broiler chickens. All analyses are on previously collected tissues. A total of 600 one day old Cobb 500 male chicks were weighed and randomly assigned to 12 environmental chambers. Ambient temperature in the chambers was gradually decreased from 32˚C on d 1 to 24˚C …

Effects Of A Noni-Supplemented Diet On Intestinal Tight Junction Proteins And Stress Biomarkers In Heat-Stressed Broiler Chickens, Guillermo Tellez

Poultry Science Undergraduate Honors Theses

Heat stress hampers gut health by impeding its ability to absorb nutrients which leads to a lower feed efficiency and sicker birds. Therefore, there is a critical need to identify mechanism-based strategies to alleviate these effects. Polynesian poultry farmers have been supplementing broiler feed with Morinda Citrifolia (Noni), a medicinal plant high in anti-oxidants, to address this problem. The purpose of the present study was to evaluate the effect of Morinda Citrifolia (Noni)-supplementation on the expression of tight junction proteins. Male Broilers (Cobb 500, 3 wks, n=480) were subjected to 2 environmental conditions (TN, 24°C vs. HS, 35°C, 1 …

Effect Of Morinda Citrifolia (Noni)-Enriched Diet On Hepatic Heat Shock Protein And Lipid Metabolism-Related Genes In Heat Stressed Broiler Chickens, Joshua Flees

Graduate Theses and Dissertations

Heat stress (HS) has been reported to alter fat deposition in broilers, however the underlying molecular mechanisms are not well-defined; therefore, the objectives of the current study were to: (1) determine the effects of acute (2 h) and chronic (3 wk) HS on the expression of key molecular signatures involved in hepatic lipogenic and lipolytic programs; and (2) assess if diet supplementation with dried Noni medicinal plant (0.2% of the diet) modulates these effects. Broilers (n=480 males, 1 d) were randomly assigned to 12 environmental chambers, subjected to two environmental conditions (HS at 35°C vs. thermoneutral condition [TN] at 24°C) …

Transcriptome Analysis In Spleen Reveals Differential Regulation Of Response To Newcastle Disease Virus In Two Chicken Lines, Jibin Zhang, Michael G. Kaiser, Melissa S. Deist, Rodrigo A. Gallardo, David A. Bunn, Terra R. Kelly, Jack C.M. Dekkers, Huaijun Zhou, Susan J. Lamont

Jibin Zhang

Experimental Approaches To Understand And Control Salmonella Infection In Poultry, Yichao Yang

Graduate Theses and Dissertations

Salmonella is a major foodborne pathogen around the world and chickens are the major reservoir to transmit Salmonella into the human food chain. For decreasing the infection of Salmonella, we developed six attenuated live vaccines based on Salmonella Enteritidis (SE) and Typhimurium (ST) for testing the cross-serovar and cross-serogroup protection from the challenge of Salmonella Heidelberg and Campylobacter jejuni. One of the constructed vaccine strain showed ability to protect against challenge from Salmonella Heidelberg. Even though some preventive approaches are able to decrease Salmonella colonization in the gastrointestinal tract of chickens or other farm animals, Salmonella transmission mechanisms remain unclear. …

Evaluating Long-Term Direct And Correlated Selection Response In White Plymouth Rock Chickens Selected For High Or Low 8-Week Body Weight, Sylvia Harrison

Department of Animal Science: Dissertations, Theses, and Student Research

The increasing demand for poultry meat has led animal breeders to engage in artificial selection of chickens as a way to increase the productivity of poultry. Long-term experiments have been designed to measure rates of genetic response to a trait under selection, and correlated traits, as well as gauge possible selection limits.

Two studies were conducted to evaluate response to selection for body weight (BW) in chickens. The chickens were selected for high or low BW at 8 weeks of age. Those that met the criterion were selected as parents for the subsequent generation. In the first study the population …

Avian And Mammalian Facilitative Glucose Transporters, Mary Shannon Byers, Christianna Howard, Xiaofei Wang

Biology Faculty Research

The GLUT members belong to a family of glucose transporter proteins that facilitate glucose transport across the cell membrane. The mammalian GLUT family consists of thirteen members (GLUTs 1–12 and H+-myo-inositol transporter (HMIT)). Humans have a recently duplicated GLUT member, GLUT14. Avians express the majority of GLUT members. The arrangement of multiple GLUTs across all somatic tissues signifies the important role of glucose across all organisms. Defects in glucose transport have been linked to metabolic disorders, insulin resistance and diabetes. Despite the essential importance of these transporters, our knowledge regarding GLUT members in avians is fragmented. It is clear that …

Effect Of Light Intensity On Production Parameters And Feeding Behavior Of Broilers, Maurice Raccoursier Frost

Graduate Theses and Dissertations

This project was performed in two parts. The first was focused on light intensity as it affects performance. A randomized complete block design (RCBD) was performed. Broilers, Cobb 500 (n = 1584) were housed in 3 commercial houses (121.9 x 12.2 m). In each house birds were randomized and placed in 72 pens of 121.9 x 121.9 cm (22 bird/pen, males and females). All the treatment groups were provided with 24h light (L) during the first week and then 18L:6Dark (D) and 20 lux from day 7 to 14. The 3 intensity treatments of 5 lux (lx), 10 lx and …

Leptin Regulates The Expression Of Autophagy-Related Genes In Chickens, Peter Olawale Ishola

Graduate Theses and Dissertations

Autophagy or cellular self-digestion, a lysosomal degradation pathway that is conserved from yeast to human, plays a key role in recycling cellular constituents, including damaged organelles. It also plays a pivotal role in the adaptation of cells to a plethora of distinct stressors including starvation. Autophagy has been extensively studied in mammals and yeast, but little is known in avian species. Thus, the major objective of the present study was to determine the effects of leptin on autophagy-related genes in chicken hypothalamus, muscle and liver. Leptin is an adipocytokine that is mostly produced by white adipose cells in mammals (as …

The Ability Of Select Probiotics To Reduce Enteric Campylobacter Colonization In Broiler Chickens, Sandip Shrestha

Graduate Theses and Dissertations

Campylobacter is the leading cause of foodborne illness worldwide and is often associated with consumption and/or mishandling of contaminated poultry products. Probiotic use in poultry has been an effective strategy in reducing other enteric foodborne pathogens but not consistently for Campylobacter. As Campylobacter resides and utilizes intestinal mucin for growth, isolates selected on the basis of mucin utilization might be a strategy to screen for efficacious probiotic bacterium. In this study, bacterial isolates demonstrating increased growth rates in mucin, in vitro (trials 1 or 2), or isolates demonstrating a reduction of Campylobacter counts when co-incubated with mucin, in vitro (trials …

Bulletin 2220: Best Management Practices For Small Scale Poultry Producers In Maine, Richard Brzozowski, Donna R. Coffin, Michael Darre

Cooperative Extension - Agriculture

Written for small-scale poultry producers. University specialists, Extension educators, and agriculture service providers from New England and New York developed this list of Best Management Practices to assist small-scale poultry growers to provide the best of care for their birds, minimize losses due to disease and predation, as well as minimizing the impact on the environment while assuring a high quality, wholesome product for their family and customers.

Efficacy Of Beta-Resorcylic Acid To Reduce Campylobacter Jejuni In Pre-Harvest And Post-Harvest Poultry, Basanta Raj Wagle

Graduate Theses and Dissertations

Campylobacteriosis is one of the leading foodborne illnesses in United States, and is associated with the consumption of poultry and poultry products. Reducing Campylobacter in these species will reduce the burden of this disease. Unfortunately, most strategies employed to reduce Campylobacter in poultry have either not been successful or produced inconsistent results. One potential control strategy is the use of β-resorcylic acid (BR), a phytophenolic compound classified by the US FDA as "Everything Added to Food in the United States" (EAF 3045) and is therefore deemed safe for consumption. This compounds has antibacterial activity against Salmonella, however, its efficacy to …

Manipulating Lipolysis To Reduce Fatness And Improve Carcass Composition In Commercial Broilers, Rodney Barnett Ray

Masters Theses

Betaine has been studied as an osmolyte and methyl group donor for many species. Recent studies have found that betaine is able to increase muscle mass and reduce adiposity in meat type broilers birds. In experiment one, eight-hundred Cobb 500 broiler chicks were supplemented with betaine at 0.6 g/kg and 1.2g/kg. Birds had access to feed and water for ad libitum consumption for forty-two, forty-four, and forty-nine days. Birds and feed were weighed weekly. Carcass parameters (dress weight, breast percentage, and leg percentage) were taken at slaughter on days forty- two and forty-nine. Blood was taken at day forty-four to …

Neurotensin And Cholecystokinin Contract Gallbladder Circular Muscle In Chickens, Teresa F. Degolier, D. R. Brown, Gary E. Duke, M. M. Palmer, J. R. Swenson, Robert E. Carraway

Biological Sciences Faculty Publications

The contractile effects of neurotensin (NT) and cholecystokinin octapeptide (CCK-8) on isolated circular smooth muscle strips of chicken gallbladder were investigated. The NT (0.25-300 nM) produced concentration-dependent contractions on smooth muscle with an EC50 of 8.5 nM (95% confidence limits = 5.3-13.6 nM). In comparison, CCK-8 produced concentration-dependent contractions with an EC50 of 13 nM (95% confidence limits of 9-20 nM). There were no statistical differences in contractile responses when comparing NT and CCK-8 at equimolar concentrations. The NT appears to act directly on smooth muscle tissue in the chicken; the contractile responses were not blocked by 10 μM atropine …

Release Of Avian Neurotensin In Response To Intraluminal Contents In The Duodenum Of Chickens, Teresa F. Degolier, Robert E. Carraway

Biological Sciences Faculty Publications

Peripheral and hepatic-portal plasma levels of neurotensin (NT) in fed and fasted chickens were determined using RIA. Portal levels of NT1-13 (fed = 61.3 ± 3.9 fmol/mL; fasted = 44.5 ± 3.9 fmol/mL) were significantly higher than peripheral levels (fed = 8.2 ± 3.3 fmol/mL; fasted = 7.8 ± 3.0 fmol/mL) collected from the wing vein, indicating that some NT is metabolized in the liver. Portal plasma levels of NT collected from fed birds were also significantly higher than portal plasma levels of NT collected from fasted birds. Neurotensin, as identified by HPLC, exhibited a 2-fold increase in plasma extracts …

Prevalence And Antimicrobial Resistance Of Pathogenic Bacteria In Chicken And Guinea Fowl, Agnes Kilonzo-Nthenge, Samuel N. Nahashon, Fur-Chi Chen, Nathaniel Adefope

Agricultural and Environmental Sciences Faculty Research

This study was conducted to compare the presence and antimicrobial susceptibility of Campylobacter, Salmonella spp., and other enteric bacteria between chickens and guinea fowls. Birds were reared on enclosed concrete floor housing covered with pine wood shavings litter material. Chicken (n = 40) and guinea fowl (n = 40) carcasses, drinking water (10 mL; n = 40), and litter (10 g; n = 40) were aseptically collected randomly from a poultry farm and analyzed within 1 h of collection. Individual pens served as experimental units and were replicated twice. Campylobacter spp., Salmonella spp., and other enterobactericeae were isolated and identified …

Eyespray Vaccination: Infectivity And Development Of Immunity To Eimeria Acervulina And Eimeria Tenella, H. David Chapman, Tim E. Cherry

Faculty Publications

The infectivity of a coccidiosis vaccine and its ability to immunize chickens against two species of Eimeria was examined. The vaccine was administered to newly-hatched chicks by spraying directly onto the eye. The method resulted in a high proportion of chicks infected with E. acervulina and E. tenella. Vaccinated birds reared in cages in the absence of reinfection did not develop immunity to either species by 4 wk of age, but birds reared in floor pens developed immunity to both E. acervulina and E. tenella.

Developing Rapid-Feathering Chickens, I. L. Williams, H. L. Wiegers

Nebraska Agricultural Experiment Station: Historical Circulars

The object of this circular is to acquaint the poultry producer, hatchery operator, or any other person endeavoring to develop improved strains of chickens with a satisfactory method for eliminating slow-feathering individuals from his flock.

Poultry Progress: What Are Good Chickens?, F. E. Mussehl

Nebraska Agricultural Experiment Station: Historical Circulars

The desire for improvement is one of the highest of human virtues. Folks everywhere want good chickens. The poultry business is a competitive enterprise, and it is imperative that we have the best stock possible for the particular job to be done. But what are good chickens? Is there confusion about the term? Perhaps we can clarify the picture by asking one or two questions.