Digital Commons Network^™

Temperature Compensation Of Auxin Dependent Developmental Patterning, Nicholas J. Kaplinsky

Biology Faculty Works

The establishment of localized auxin gradients plays a central role in developmental patterning in plants. Auxin levels and responses have been shown to increase with temperature although developmental patterning is not affected. This suggests the existence of a homeostatic mechanism that ensures that patterning occurs normally over a range of temperatures. We recently described the cloning and characterization of BOBBER1 (BOB1), an Arabidopsis gene which encodes a small heat shock protein. BOB1 is required for the establishment of auxin gradients and for normal developmental patterning. BOB1 is also required for organismal thermotolerance and localizes to heat shock granules at elevated …

Techniques For Triggering Germination Of Adenostoma Fasciculatum In Revegetation Projects At Rocky Canyon Quarry, Stephanie B. Scolari

Master's Theses

Rocky Canyon Quarry is a granite mine located in the Santa Lucia Mountains about 5 miles southeast of the city of Atascadero. The Surface Mining and Reclamation Act (SMARA), which passed in 1975, dictates that California mines must rehabilitate lands disturbed by mining operations. Dr. V. L. Holland and his students in the Biological Sciences Department at California Polytechnic State University, San Luis Obispo (Cal Poly) have been working on restoration and revegetation of the Rocky Canyon Quarry since the early 1990’s.

Adenostoma fasciculatum (chamise) dominates the chaparral communities (chamisal chaparral) found in Rocky Canyon. Chamise is the most common …

Remote Sensing Of Harmful Algal Blooms In The Mississippi Sound And Mobile Bay: Modelling And Algorithm Formation, Dan Martin Holiday

Dissertations

The incidence and severity of harmful algal blooms have increased in recent decades, as have the economic effects of their occurrence./The diatom Pseudo-nitzschia spp. caused fisheries closures in Mobile Bay during 2005 due to elevated levels of domoic acid. In the previous 4 years Karenia brevis counts of >5,000 cells L"1 have occurred in Mobile Bay and the Mississippi Sound. Population levels of this magnitude had previously been recorded only in 1996. Increases in human populations, urban sprawl, development of shoreline properties, sewage effluent and resultant changes in NP ratios of discharge waters, and decline in forest and marsh lands, …

Light-Related Photosynthetic Gene Expression And Enzyme Activity In The Heterokont Alga Vaucheria Litorea And Its Symbiotic Partner The Sacoglossan Mollusc Elysia Chlorotica, Kara M. Soule

Electronic Theses and Dissertations

Photosynthesis is comprised of tightly coupled reactions and therefore requires strict matrices of regulation, particularly involving alterations in gene expression and enzyme activity within the nucleus and plastid. Extensive research has been carried out on these light-regulated mechanisms in plants and green algae, however, much less is known in the red algal lineage, including heterokonts. The goal of this study was to investigate the influence of light on photosynthetic gene expression and select enzyme activity in the heterokont alga Vaucheria litorea and its symbiotic partner Elysia chlorotica, a photosynthetic sacoglossan mollusc (sea slug). Elysia chlorotica harbors V. litorea plastids …

Long Distance Molt Migration By A Giant Canada Goose From Eastern South Dakota, Bobby J. Anderson, Charles D. Dieter

The Prairie Naturalist

To reduce crop damage by resident giant Canada geese (Schaible et al. 2005), the South Dakota Department of Game, Fish and Parks (SDGFP) initiated a program to reduce goose nesting success in eastern South Dakota. One management tool used by SDGFP personnel was the destruction of giant Canada goose nests. When a giant Canada goose has its nest destroyed, they are known to initiate a molt migration (Mykut 2002, Luukkonen et aI. 2008). We attached Platform Transmitting Terminals (PTT; model ST -19) to document and describe molt migrations of giant Canada geese following nest destruction. We captured 3 adult nesting …

Detection And Measurement Of Water Stress In Vegetation Using Visible Spectrum Reflectance, Arthur Zygielbaum

Department of Geography: Dissertations, Theses, and Student Research

At any scale, from a single microbe to the planet that nurtures us, water defines our place in the universe. It provides the hydraulic forces needed to give plants structure, and the medium enabling photosynthesis, the basis for most life on Earth, to occur. Knowledge of plant water status is vital to understanding the state or condition of vegetation, information which is essential to disciplines as diverse as agriculture, geography, and climatology. Non-destructive and remote sensing of plant water status allows the gathering of such information across wide geographic extents and over long periods of time. Monitoring vegetation remotely requires …

Herbicide, Salinity, And Flooding Tolerance Of Foxtail Barley (Hordeum Jubatum L.) And Desirable Pasture Grasses, Karl R. Israelsen

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Research trials performed in the greenhouse compared the tolerance and response of Hordeum jubatum and desirable pasture grass species to herbicides, salinity, and flooding. Desirable grass species used in this study included: 'Fawn' tall fescue (Festuca arundinaceae), 'Garrison' creeping foxtail (Alopecurus arundinaceus), 'Palaton' reed canarygrass (Phalaris arundinacea), 'Climax' timothy (Phleum pratense), 'Alkar' tall wheatgrass (Thinopyrum ponticum), 'Potomac' orchardgrass (Dactylis glomerata), and 'Mustang' altai wildrye (Leymus angustus). Tolerance to herbicides, salinity, and flooding varied significantly among grass species. Herbicide tolerance was tested using four herbicides at five …

Water Uptake Threshold Of Rabbiteye (Vaccinium Ashei) Blueberries And Its Influence On Fruit Splitting, Donna A. Marshall-Shaw, James M. Spiers, Kenneth J. Curry

Faculty Publications

Split-resistant and split-susceptible rabbiteye blueberry fruit were evaluated at all stages of development to determine ''water uptake thresholds'' by soaking in distilled water. Weight increase after soaking was measured, and percent weight gain was calculated to take into consideration the weight increase of the fruit from development. The ratio of percent increase in volume to weight increase resulting from water uptake was calculated. Ratios of percent water uptake to weight increase between splitsusceptible 'Tifblue' and split-resistant 'Premier' blueberries were found to be similar. The split-susceptible 'Tifblue' had a 1.6 g/50 fruit increase with a 1.7% water uptake and a ratio …

Arkansas Corn And Grain Sorghum Performance Tests 2009, D. G. Dombeck, R. D. Bond, I. L. Eldridge, R. M. Pryor

Arkansas Agricultural Experiment Station Research Series

Corn and grain sorghum performance tests are conducted each year in Arkansas by the University of Arkansas Division of Agriculture. The tests provide information to companies marketing seed within the state and aid the Arkansas Cooperative Extension Service in formulating recommendations for producers.

Skeleton Weed In Western Australia, Pocket Guide, State Skeleton Weed Committee, Department Of Agriculture And Food, Western Australia, Agriculture Protection Board

Bulletins 4000 -

The Skeleton Weed Program is a coordinated approach to eradicating skeleton weed in Western Australia. It is run jointly by the State Skeleton Weed Committee, the Department of Agriculture and the Agriculture Protection Board. It is funded through the skeleton weed levy collected from grain growers each harvest, and pooled in the Skeleton Weed Eradication Trust Fund.

Skeleton weed is targeted as a declared plant as it can drastically reduce crop yields (more than 1 tonne/ha in cereals) by competing for moisture and nutrients, mainly nitrogen. Its wiry stems impede headers and the sticky latex they contain cause machinery to …

Discovering Metabolic Networks Of Bovine Fertilization, Erin Lynn Young

Undergraduate Honors Capstone Projects

At the time of fertilization, a dramatic change occurs in the oocyte that transforms this cell from a metaphase arrested state into a metabolically active and dynamic state. The view of the flow of biological processes within organisms has recently shifted from that of a linear path to a more complex network. Biological processes are no longer thought of in the simple terms of DNA to RNA, RNA to proteins, and proteins to final activity. It is now known that many biological processes involve interconnected networks and feedback loops in which DNA, RNA, proteins, and metabolites perform specific roles. We …

Impacts Of American Student Teachers On Twelve Community Members In A Rural New South Wales Community Australia: A Qualitative Study, Tera Shenae Bunch

Masters Theses

The purpose of this study was to evaluate the influences of American student teachers on a rural community in New South Wales, Australia. The study analyzed interviews with twelve participants of the American student teacher program in A Rural New South Wales community, Australia. Two researchers worked together to complete this study. Both researchers were student teachers with the New South Wales community for ten weeks and taught Agriculture in one the community‟s two high schools. After allowing one year to pass, researcher one returned to the community to interview twelve individuals involved with the program. The interviewed participants were …

Heirloom And Hybrid Tomato Yield And Quality In Organic And Conventional Production Systems, Diana J. Edlin

Masters Theses & Specialist Projects

Due to the recent changes in the economy of Kentucky tobacco production, some producers are seeking an alternative crop that will provide similar economic gains to tobacco without needing more acreage. Tomatoes are an existing crop in Kentucky that have been declining in acreage over the last five years. There is evidence to suggest that, through niche and local marketing, tomatoes may be able to fill the void left by tobacco. However, there is concern among producers that they will lose yield and/or quality if they switch to one of these niche production systems or cultivars.

A two year study …

In Planta "Green Engineering" Of Variable Sizes And Exotic Shapes Of Gold Nanoparticles: An Integrative Eco-Friendly Approach, Daniel Lee Starnes

Masters Theses & Specialist Projects

Manipulating matter at the nanoscale creates materials endowed with unique optoelectronic and physicochemical attributes. Among the noble metals, the properties of gold in "nano" can be manipulated by varying, their shapes and sizes. Gold nanoparticles find several applications in electronics, medicine and environmental reclamation. Emphasis has been on the “green synthesis” of nanogold to mitigate the hazardous implications stemmed from conventional nanogold synthesis. However, it is not known if the in planta synthesis of nanogold particles could be “green engineered” as well for generating desirable sizes and exotic shapes. In the present study, we used inductively coupled plasma (ICP) analysis …

Mixed Species Plantations: Prospects And Challenges, J Doland Nichols, Mila Bristow, Jerome K. Vanclay

Professor Jerome K Vanclay

About 2% of English-language literature on plantations deals with mixed-species plantations, but only a tiny proportion (<0.1%) of industrial plantations are polycultures. Small landholders are more innovative, with 12% of Australia’s farm forestry plantations under mixed-species plantings, and 80% of Queensland’s farm forestry as polycultures. We examine reasons for this discrepancy, and explore the history, silviculture and economics of polycultures. Financial analyses suggest that a yield stimulus of 10%, depending on product and rotation length, may be sufficient to offset increased costs associated with planting and managing a mixed-species plantation, a stimulus that has been demonstrated in many field …

Improving Productivity In Mixed-Species Plantations, Mila Bristow, J Doland Nichols, Jerome K. Vanclay

Professor Jerome K Vanclay

Mixed species plantations are often promoted as being environmentally preferable to monocultures, but are rarely considered operationally viable by commercial forest growers. Despite many publications documenting benefits demonstrated in research studies (e.g., Kelty 2006; Forrester et al. 2006b; Wood and Vanclay 1995), and despite continuing calls from a wide range of advocates for mixed-species plantations, polyculture remains the exception rather than the rule in industrial plantation forestry (Nichols et al 2006)...

Characterization Of Genes Encoding Poly(A) Polymerases In Plants: Evidence For Duplication And Functional Specialization, Lisa R. Meeks, Bala Subrahmanyam Addepalli, Arthur G. Hunt

Plant and Soil Sciences Faculty Publications

Background: Poly(A) polymerase is a key enzyme in the machinery that mediates mRNA 3′ end formation in eukaryotes. In plants, poly(A) polymerases are encoded by modest gene families. To better understand this multiplicity of genes, poly(A) polymerase-encoding genes from several other plants, as well as from Selaginella, Physcomitrella, and Chlamydomonas, were studied.

Methodology/Principal Findings: Using bioinformatics tools, poly(A) polymerase-encoding genes were identified in the genomes of eight species in the plant lineage. Whereas Chlamydomonas reinhardtii was found to possess a single poly(A) polymerase gene, other species possessed between two and six possible poly(A) polymerase genes. With the exception …

Non-Antibiotic Selection Systems For Soybean Somatic Embryos: The Lysine Analog Aminoethyl-Cysteine As A Selection Agent, Suryadevara S. Rao, Lewamy Mamadou, Matt Mcconnell, Raghuveer Polisetty, Prachuab Kwanyuen, David Hildebrand

Plant and Soil Sciences Faculty Publications

BACKGROUND: In soybean somatic embryo transformation, the standard selection agent currently used is hygromycin. It may be preferable to avoid use of antibiotic resistance genes in foods. The objective of these experiments was to develop a selection system for producing transgenic soybean somatic embryos without the use of antibiotics such as hygromycin.

RESULTS: When tested against different alternate selection agents our studies show that 0.16 microg/mL glufosinate, 40 mg/L isopropylamine-glyphosate, 0.5 mg/mL (S-(2 aminoethyl)-L-cysteine) (AEC) and the acetolactate synthase (ALS) inhibitors Exceed and Synchrony both at 150 microg/mL inhibited soybean somatic embryo growth. Even at the concentration of 2 mg/mL, …

Viral Vectors Useful In Soybean And Methods Of Use, Said A. Ghabrial, Chunquan Zhang, Honcgang Gu

Plant Pathology Faculty Patents

The invention provides Bean pod mottle virus (BPMV) vectors useful for expression of heterologous proteins in plants such as soybean. The BPMV vectors are also useful for virus-induced gene silencing. The invention also provides methods for expressing a heterologous polypeptide in a plant such as soybean. The invention additionally provides methods for virus-induced gene silencing, particularly in a soybean plant, which can be used to determine the function of a gene of interest.

Baird, Henry Herring, 1902-1991 - Collector (Mss 279), Manuscripts & Folklife Archives

Manuscript Collection Finding Aids

Finding aid only for Manuscripts Collection 279. Account books and other financial records related to Jenkins-Ryan Tobacco Company of Adairville, Kentucky. Also includes financial records associated with the farms and businesses operated by Thomas Henry Baird, Jr., as well as the 1941 monthly financial reports and a label from Scott Tobacco Company of Bowling Green, Kentucky.

Defective Interfering Rnas: Foes Of Viruses And Friends Of Virologists, Kunj Bihari Pathak, Peter D. Nagy

Plant Pathology Faculty Publications

Defective interfering (DI) RNAs are subviral RNAs produced during multiplication of RNA viruses by the error-prone viral replicase. DI-RNAs are parasitic RNAs that are derived from and associated with the parent virus, taking advantage of viral-coded protein factors for their multiplication. Recent advances in the field of DI RNA biology has led to a greater understanding about generation and evolution of DI-RNAs as well as the mechanism of symptom attenuation. Moreover, DI-RNAs are versatile tools in the hands of virologists and are used as less complex surrogate templates to understand the biology of their helper viruses. The ease of their …

Seed Dispersal And Reproduction Patterns Among Everglades Plants, Ronald E. Mossman

FIU Electronic Theses and Dissertations

In this study three aspects of sexual reproduction in Everglades plants were examined to more clearly understand seed dispersal and the allocation of resources to sexual reproduction— spatial dispersal process, temporal dispersal of seeds (seedbank), and germination patterns in the dominant species, sawgrass (Cladium jamaicense). Community assembly rules for fruit dispersal were deduced by analysis of functional traits associated with this process. Seedbank ecology was investigated by monitoring emergence of germinants from sawgrass soil samples held under varying water depths to determine the fate of dispersed seeds. Fine-scale study of sawgrass fruits yielded information on contributions to variation in sexually …

Functional Transition In The Floral Receptacle Of The Sacred Lotus (Nelumbo Nucifera): From Thermogenesis To Photosynthesis, R. E. Miller, J. R. Watling, Sharon A. Robinson

Sharon Robinson

The receptacle of the sacred lotus is the main source of heat during the thermogenic stage of floral development. Following anthesis, it enlarges, greens and becomes a fully functional photosynthetic organ. We investigated development of photosynthetic traits during this unusual functional transition. There were two distinct phases of pigment accumulation in receptacles. Lutein and photoprotective xanthophyll cycle pigments accumulated first with 64% and 95% of the maximum, respectively, present prior to anthesis. Lutein epoxide comprised 32% of total carotenoids in yellow receptacles, but declined with development. By contrast, more than 85% of maximum total chlorophyll, β-carotene and Rubisco were produced …

Responses Of Plants In Polar Regions To Uvb Exposure : A Meta-Analysis, K. K. Newsham, Sharon A. Robinson

Sharon Robinson

We report a meta-analysis of data from 34 field studies into the effects of UV-B radiation on Arctic and Antarctic bryophytes and angiosperms. The studies measured plant responses to decreases in UV-B radiation under screens, natural fluctuations in UV-B irradiance, or increases in UV-B radiation applied from fluorescent UV lamps. Exposure to UV-B radiation was found to increase the concentrations of UV-B absorbing compounds in leaves or thalli by 7% and 25% (expressed on a mass or area basis, respectively). UV-B exposure also reduced aboveground biomass and plant height by 15% and 10%, respectively, and increased DNA damage by 90%. …

Genetic Structure Of East Antarctic Populations Of The Moss Ceratodon Purpureus, L. J. Clarke, D. J. Ayre, Sharon A. Robinson

Sharon Robinson

The capacity of the polar flora to adapt is of increasing concern given current and predicted environmental change in these regions. Previous genetic studies of Antarctic mosses have been of limited value due to a lack of variation in the markers or non-specificity of the methods used. We examined the power of five microsatellite loci developed for the cosmopolitan moss Ceratodon purpureus to detect genetically distinct clones and infer the distribution of clones within and among populations from the Windmill Islands, East Antarctica. Our microsatellite data suggest extraordinarily high levels of variation reported in RAPD studies were artificially elevated by …

Two Cys Or Not Two Cys? That Is The Question; Alternative Oxidase In The Thermogenic Plant Sacred Lotus, Nicole M. Grant, Yoshihiko Onda, Yusuke Kakizaki, Kkikukatsu Ito, Jennifer R. Watling, Sharon A. Robinson

Sharon Robinson

Sacred lotus (Nelumbo nucifera) regulates temperature in its floral chamber to 32°C to 35°C across ambient temperatures of 8°C to 40°C with heating achieved through high alternative pathway fluxes. In most alternative oxidase (AOX) isoforms, two cysteine residues, Cys1 and Cys2, are highly conserved and play a role in posttranslational regulation of AOX. Further control occurs via interaction of reduced Cys1 with α-keto acids, such as pyruvate. Here, we report on the in vitro regulation of AOX isolated from thermogenic receptacle tissues of sacred lotus. AOX protein was mostly present in the reduced form, and only a small fraction could …

Biological Control Of Purple Loosestrife (Lythrum Salicaria): Factors Affecting Galerucella Pusilla And Galerucella Calmariensis Establishment In Tidal Areas, Lynda Kathryn Moore

Dissertations and Theses

Galerucella pusilla and G. calmariensis have provided successful biological control of purple loosestrife (Lythrum salicaria L.) in non-tidal areas but only marginal control in areas of tidal influence. While a previous study identified mechanical scour by tidal waters as the main cause of establishment failure, purple loosestrife stem density explained more than 80% of the variability in presence and absence of Galerucella at my study sites in the Columbia River Estuary. A logistic regression model using purple loosestrife stem density, elevation, and their interaction as predictors accurately predicted 92.5% of Galerucella presence or absence observations of a test data …

Annis, Lena Grey, 1897-1996 - Collection (Mss 275), Manuscripts & Folklife Archives

Manuscript Collection Finding Aids

Finding aid only for Manuscripts Collection 275. Letters sent to Lena Grey Annis from family members and friends, chiefly related to farm and family life in Butler County, Kentucky in the mid-to-late-twentieth century.

Identifying Sub-Tropical Grass Seedlings, Brad Wintle, Geoff Moore, Phil Nichols

Bulletins 4000 -

There is considerable interest in growing sub-tropical (or warm season) perennial grasses for out-of-season green feed, especially on the south coast and in the northern agricultural region (NAR). They consist of two types: creeping grasses, which spread by either above ground runners (stolons) or below ground runners (rhizomes) and bunch grasses, which are tufted and do not spread by runners.

The main species sown include Panic grass, Rhodes grass, Kikuyu, Setaria, Signal grass, Digit grass, and Bambatsi panic and are often sown as a mixture, comprising 2 or more species. This bulletin describes how to identify these grasses at the …

Corn Response To Competition: Growth Alteration Vs. Yield Limiting Factors, Sharon A. Clay, David E. Clay, D. P. Horvath, J. Pullis, C. Gregg Carlson, Stephanie A. Hansen, Graig Reicks

Agronomy, Horticulture and Plant Science Faculty Publications

Competition mechanisms among adjacent plants are not well understood. This study compared corn growth and yield responses to water, N, and shade at 74,500 plants ha⁻¹ (1×) with responses to water and N when planted at 149,000 plant ha⁻¹ Plant biomass, leaf area, chlorophyll content, reflectance, and enzyme expression (transcriptome analysis) were measured at V-12. Grain and stover yields were measured with grain analyzed for ¹³C isotopic discrimination (Δ) and N concentration. At V-12, 60% shade plants had increased chlorophyll and reduced leaf area and height compared to full sun plants. In the 2× treatment, plants had …