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Center for Plant Science Innovation: Faculty and Staff Publications
TGS transcriptional gene silencing; PTGS posttranscriptional gene silencing; DSBs DNA double-strand breaks; TAP Tris-acetate-phosphate; UV-C UV light <280 nm
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Suppressors Of Transcriptional Transgenic Silencing In Chlamydomonas Are Sensitive To Dna-Damaging Agents And Reactivate Transposable Elements, Byeong-Ryool Jeong, Dancia Wu-Scharf, Chaomei Zhang, Heriberto D. Cerutti
Suppressors Of Transcriptional Transgenic Silencing In Chlamydomonas Are Sensitive To Dna-Damaging Agents And Reactivate Transposable Elements, Byeong-Ryool Jeong, Dancia Wu-Scharf, Chaomei Zhang, Heriberto D. Cerutti
Center for Plant Science Innovation: Faculty and Staff Publications
In the unicellular green alga Chlamydomonas reinhardtii, the epigenetic silencing of transgenes occurs, as in land plants, at both the transcriptional and posttranscriptional levels. In the case of single-copy transgenes, transcriptional silencing takes place without detectable cytosine methylation of the introduced DNA. We have isolated two mutant strains, Mut-9 and Mut-11, that reactivate expression of a transcriptionally silenced single-copy transgene. These suppressors are deficient in the repression of a DNA transposon and a retrotransposon-like element. In addition, the mutants show enhanced sensitivity to DNA-damaging agents, particularly radiomimetic chemicals inducing DNA double- strand breaks. All of these phenotypes are much …