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United States Patent Application Publication: Isolation And Characterization Of Heat Shock Protein, Thomas Elthon, Adrian A. Lund, Dinakar Bhattramakki, David Rhoads
United States Patent Application Publication: Isolation And Characterization Of Heat Shock Protein, Thomas Elthon, Adrian A. Lund, Dinakar Bhattramakki, David Rhoads
Center for Plant Science Innovation: Faculty and Staff Publications
This invention relates to the identification and characterization of the maize HSP22 protein including the corresponding nucleic acid molecules, nucleic acid fragments and protein fragments. Further, this invention relates to both polyclonal and monoclonal antibodies to HSP22 and the production thereof. Those antibodies can be used in a method for detecting the presence of HSP22 expression in whole leaf samples. Further, the nucleic acid molecule and fragments thereof can be used in assays to detect the levels of nucleic acid molecules involved in HSP22 expression. The levels of both HSP22 and HSP22 mRNA are then utilized as an indication of …
A Wd40-Repeat Containing Protein, Similar To A Fungal Co-Repressor, Is Required For Transcriptional Gene Silencing In Chlamydomonas, Chaomei Zhang, Dancia Wu-Scharf, Byeong-Ryool Jeong, Heriberto D. Cerutti
A Wd40-Repeat Containing Protein, Similar To A Fungal Co-Repressor, Is Required For Transcriptional Gene Silencing In Chlamydomonas, Chaomei Zhang, Dancia Wu-Scharf, Byeong-Ryool Jeong, Heriberto D. Cerutti
Center for Plant Science Innovation: Faculty and Staff Publications
In higher plants, mammals, and filamentous fungi, transcriptional gene silencing is frequently associated with DNA methylation. However, recent evidence suggests that certain transgenes can be inactivated by a methylation independent mechanism. In the unicellular green alga Chlamydomonas reinhardtii, single-copy transgenes are transcriptionally silenced without discernible cytosine methylation of the introduced DNA. We have isolated a Chlamydomonas gene, Mut11, which is required for the transcriptional repression of single-copy transgenes. Mut11 appears to have a global role in gene regulation since it also affects transposon mobilization, cellular growth, and sensitivity to DNA damaging agents. In transient expression assays, a fusion …
United States Patent Application Publication: Counter Selection Strategy For Gram-Negative Bacteria, Stephen K. Farrand, Paul E. Staswick, Thomas E. Clemente
United States Patent Application Publication: Counter Selection Strategy For Gram-Negative Bacteria, Stephen K. Farrand, Paul E. Staswick, Thomas E. Clemente
Center for Plant Science Innovation: Faculty and Staff Publications
A Gram-negative bacterium useful for genetically engineering plants is provided. The Gram-negative bacterium contains, as part of genome, an inducible regulatory sequence operatively linked to a nucleotide sequence encoding a levansucrase. Alternatively, the Gram-negative bacterium comprises a recombinant nucleic acid construct containing an inducible regulatory sequence operatively linked to a nucleotide sequence encoding a levansucrase. Also provided are recombinant nucleic acid constructs comprising an inducible regulatory sequence operatively coupled to a nucleotide sequence encoding a levansucrase and a method for transforming plants using the Gram-negative bacterium of the present invention.
United States Patent Application Publication: Down-Regulation Of Single Genes And Simultaneous Down-Regulation Of Multiple Genes By Nuclear Localization Of Rna Transcripts, Thomas E. Clemente, Paul E. Staswick, Tony L. Buhr
United States Patent Application Publication: Down-Regulation Of Single Genes And Simultaneous Down-Regulation Of Multiple Genes By Nuclear Localization Of Rna Transcripts, Thomas E. Clemente, Paul E. Staswick, Tony L. Buhr
Center for Plant Science Innovation: Faculty and Staff Publications
A method is provided for gene suppression in eukaryotes by transformation with a recombinant construct containing a promoter, at least one antisense and/or sense nucleotide sequence for the gene(s) to be suppressed, wherein the nucleus-to-cytoplasm transport of the transcription products of the construct is inhibited. In one embodiment, nucleus-to- cytoplasm transport is inhibited by the absence of a normal 3' UTR. The construct can optionally include at least one self-cleaving ribozyme. The construct can also optionally include sense and/or antisense sequences to multiple genes that are to be simultaneously down-regulated using a single promoter. Also disclosed are vectors, plants, animals, …
The Shca Protein Is A Molecular Chaperone That Assists In The Secretion Of The Hoppsya Effector From The Type Iii (Hrp) Protein Secretion System Of Pseudomonas Syringae, Karin V. Van Dijk, Vincent C. Tam, Angela R. Records, Tanja Petnicki-Ocwieja, James R. Alfano
The Shca Protein Is A Molecular Chaperone That Assists In The Secretion Of The Hoppsya Effector From The Type Iii (Hrp) Protein Secretion System Of Pseudomonas Syringae, Karin V. Van Dijk, Vincent C. Tam, Angela R. Records, Tanja Petnicki-Ocwieja, James R. Alfano
Center for Plant Science Innovation: Faculty and Staff Publications
Pseudomonas syringae uses a type III protein secretion system encoded by the Hrp pathogenicity island (Pai) to translocate effector proteins into plant cells. One of these effector proteins is HopPsyA. A small open reading frame (ORF), named shcA, precedes the hopPsyA gene in the Hrp Pai of P. s. syringae 61. The predicted amino acid sequence of shcA shares general characteristics with chaperones used in type III protein secretion systems of animal pathogens. A functionally non-polar deletion of shcA in P. s. syringae 61 resulted in the loss of detectable HopPsyA in supernatant fractions, consistent with ShcA acting as a …
Genomewide Identification Of Pseudomonas Syringae Pv.Tomato Dc3000 Promoters Controlled By The Hrpl Alternative Sigma Factor, Derrick E. Fouts, Robert B. Abramovitch, James R. Alfano, Angela M. Baldo, C. Robin Buell, Samuel Cartinhour, Arn K. Chatterjee, Mark D'Ascenzo, Michelle L. Gwinn, Sondra G. Lazarowitz, Na-Chun Lin, Gregory B. Martin, Amos H. Rehm, David J. Schneider, Karin V. Van Dijk, Xiaoyan Tang, Alan Collmer
Genomewide Identification Of Pseudomonas Syringae Pv.Tomato Dc3000 Promoters Controlled By The Hrpl Alternative Sigma Factor, Derrick E. Fouts, Robert B. Abramovitch, James R. Alfano, Angela M. Baldo, C. Robin Buell, Samuel Cartinhour, Arn K. Chatterjee, Mark D'Ascenzo, Michelle L. Gwinn, Sondra G. Lazarowitz, Na-Chun Lin, Gregory B. Martin, Amos H. Rehm, David J. Schneider, Karin V. Van Dijk, Xiaoyan Tang, Alan Collmer
Center for Plant Science Innovation: Faculty and Staff Publications
The ability of Pseudomonas syringae pv. tomato DC3000 to parasitize tomato and Arabidopsis thaliana depends on genes activated by the HrpL alternative sigma factor. To support various functional genomic analyses of DC3000, and specifically, to identify genes involved in pathogenesis, we developed a draft sequence of DC3000 and used an iterative process involving computational and gene expression techniques to identify virulence-implicated genes downstream of HrpLresponsive promoters. Hypersensitive response and pathogenicity (Hrp) promoters are known to control genes encoding the Hrp (type III protein secretion) machinery and a few type III effector proteins in DC3000. This process involved (i) identification of …
Suppressors Of Transcriptional Transgenic Silencing In Chlamydomonas Are Sensitive To Dna-Damaging Agents And Reactivate Transposable Elements, Byeong-Ryool Jeong, Dancia Wu-Scharf, Chaomei Zhang, Heriberto D. Cerutti
Suppressors Of Transcriptional Transgenic Silencing In Chlamydomonas Are Sensitive To Dna-Damaging Agents And Reactivate Transposable Elements, Byeong-Ryool Jeong, Dancia Wu-Scharf, Chaomei Zhang, Heriberto D. Cerutti
Center for Plant Science Innovation: Faculty and Staff Publications
In the unicellular green alga Chlamydomonas reinhardtii, the epigenetic silencing of transgenes occurs, as in land plants, at both the transcriptional and posttranscriptional levels. In the case of single-copy transgenes, transcriptional silencing takes place without detectable cytosine methylation of the introduced DNA. We have isolated two mutant strains, Mut-9 and Mut-11, that reactivate expression of a transcriptionally silenced single-copy transgene. These suppressors are deficient in the repression of a DNA transposon and a retrotransposon-like element. In addition, the mutants show enhanced sensitivity to DNA-damaging agents, particularly radiomimetic chemicals inducing DNA double- strand breaks. All of these phenotypes are much …
Folate Synthesis In Plants: The First Step Of The Pterin Branch Is Mediated By A Unique Bimodular Gtp Cyclohydrolase I, Gilles J. C. Basset, Eoin P. Quinlivan, Michael J. Ziemak, Rocío Díaz De La Garza, Markus Fischer, Susi Schiffmann, Adelbert Bacher, Jesse F. Gergory Iii, Andrew D. Hanson
Folate Synthesis In Plants: The First Step Of The Pterin Branch Is Mediated By A Unique Bimodular Gtp Cyclohydrolase I, Gilles J. C. Basset, Eoin P. Quinlivan, Michael J. Ziemak, Rocío Díaz De La Garza, Markus Fischer, Susi Schiffmann, Adelbert Bacher, Jesse F. Gergory Iii, Andrew D. Hanson
Center for Plant Science Innovation: Faculty and Staff Publications
GTP cyclohydrolase I (GCHI) mediates the first and committing step of the pterin branch of the folate-synthesis pathway. In microorganisms and mammals, GCHI is a homodecamer of ≈26-kDa subunits. Genomic approaches identified tomato and Arabidopsis cDNAs specifying ≈50-kDa proteins containing two GCHI-like domains in tandem and indicated that such bimodular proteins occur in other plants. Neither domain of these proteins has a full set of the residues involved in substrate binding and catalysis in other GCHIs. The tomato and Arabidopsis cDNAs nevertheless encode functional enzymes, as shown by complementation of a yeast fol2 mutant and by assaying GCHI activity …