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Multiple Virus Infections In The Honey Bee And Genome Divergence Of Honey Bee Viruses, Yanping Chen, Yanping Zhao, John Hammond, Hei-Ti Hsu, Jay Evans, Mark Feldlaufea
Multiple Virus Infections In The Honey Bee And Genome Divergence Of Honey Bee Viruses, Yanping Chen, Yanping Zhao, John Hammond, Hei-Ti Hsu, Jay Evans, Mark Feldlaufea
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
Using uniplex RT-PCR we screened honey bee colonies for the presence of several bee viruses, including black queen cell virus (BQCV), deformed wing virus (DWV), Kashmir bee virus (KBV), and sacbrood virus (SBV), and described the detection of mixed virus infections in bees from these colonies. We report for the first time that individual bees can harbor four viruses simultaneously. We also developed a multiplex RT-PCR assay for the simultaneous detection of multiple bee viruses. The feasibility and specificity of the multiplex RT-PCR assay suggests that this assay is an effective tool for simultaneous examination of mixed virus infections in …