Digital Commons Network^™

Expression Of The Tobacco Β- 1,3-Glucanase Gene, Pr-2d, Following Induction Of Sar With Peronospora Tabacina, Deanna L. Funnell, Christopher B. Lawrence, Jeffrey F. Pedersen, Christopher L. Schardl

Department of Plant Pathology: Faculty Publications

Systemic acquired resistance (SAR) is induced following inoculation of Peronospora tabacina sporangia into the stems of Nicotiana tabacum plants highly susceptible to the pathogen. Previous results have shown that accumulation of acidic P-1,3-glucanases (PR-2's) following induction of SAR by P. tabacina may contribute to resistance to P. tabacina. We showed that up-regulation of the PR-2 gene, PR-2d, following stem inoculation with P. tabacina, is associated with SAR. Studies using plants transformed with GUS constructs containing the full length promoter from PR-2d or promoter deletions, provided evidence that a previously characterized regulatory element that is involved in response to …

Atrazine Runoff In The Blue River Basin: Geomorphology, Rainfall, And Agronomic Practices, Kundan Dhakal

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Atrazine concentrations in the Big Blue River Basin (BRB) in Nebraska and Kansas periodically exceed the U.S. EPA Maximum Contaminant Level (MCL) of 3 µg L^-1. The present study is focused on watershed variables influencing surface runoff of atrazine. The assessment includes the impact of corn and sorghum planting progress (indicating atrazine application), rainfall, antecedent soil water content, and soil restrictive layer on stream-measured weekly atrazine load in independent BRB subwatersheds for 1997 - 2004. Maximum atrazine loading occurred after most of the corn had been planted but during sorghum planting from mid-May to early June, immediately following …

Center For Grassland Studies Newsletter, Winter 2004, Volume 10, No. 1

Center for Grassland Studies: Newsletters

Matching Beef Cattle Genetics to Feed Resources by Jim Gosey, Department of Animal Science, UNL

Ecological Monitoring to Assist Producers with Grassland Production Forecasts by George Green, School of Natural Resources, UNL

Nebraska Ranch Practicum Enters Sixth Year by Jerry Volesky, Don Adams, Richard Clark, Dillon Feuz, Patrick Reece, Brent Plugge, Bud Stolzenburg and Troy Walz, UNL

Bull Riders for Biodiversity? – And Other Heresies for the Northern Great Plains by Tyler Sutton, for The Conservation Alliance of the Great Plains

2004 Nebraska Grazing Conference to be Aug. 10-11

The Nebraska Turfgrass Conference: 42 Years of Educating Nebraska Turfgrass Managers …

Agronomic Responses Of Corn Hybrids From Different Eras To Deficit And Adequate Levels Of Water And Nitrogen, Patrick M. O'Neill, J.F. Shanahan, James S. Schepers, Bob Caldwell

Department of Agronomy and Horticulture: Faculty Publications

Maintaining current high yields of corn (Zea mays L.) grown in the USA poses an environmental threat due to continued overuse of water and N inputs. To reduce overreliance on inputs, future corn breeding efforts should focus on improving tolerance of corn to water and N stresses, utilizing appropriate tolerance mechanisms. The objective of this study was to identify appropriate mechanisms by characterizing agronomic responses of 12 hybrids from three different eras (‘B73 x Mo17’ from 1970s and three early 1990s and eight late 1990s Pioneer brand hybrids) to varying water and N supply. This was done by growing …

The Prairie Naturalist Volume 36, No.4 December 2004

The Prairie Naturalist

ROADSIDE BIAS IN POINT COUNT SURVEYS AT ARROWWOOD NATIONAL WILDLIFE REFUGE, NORTH DAKOTA ▪ J. S. Dieni and P. Scherr

HISTORY OF GREATER SAGE-GROUSE IN THE DAKOTAS: DISTRIBUTION AND POPULATION TRENDS ▪ . J. T. Smith, L. D. Flake, K. F. Higgins, and G. D. Kobriger

PATCH USE BY A MONOPHAGOUS HERBIVORE IN FRAGMENTED PRAIRIE LANDSCAPES ▪ . M. J. St. Pierre and S. D. Hendrix

SNOWSHOE HARE OCCURRENCE AND WINTER HABITAT ON THE MISSOURI COTEAU IN NORTH DAKOTA ▪ R. K. Murphy 243

HIGH ABUNDANCE OF NESTING LONG-EARED OWLS IN NORTH DAKOTA ▪ R. K. Murphy, L. J. Rosenfield, …

Sources Of Plant Materials For Land Rehabilitation, Heidi Howard, John A. Guretzky, Brandy Koch, Shannon Mcdowell, Steven Warren, Gwyn L. Howard

Department of Agronomy and Horticulture: Faculty Publications

Military training often results in environmental degradation that has negative impacts on future training missions. The U.S. Army Construction Engineering Research Laboratory (CERL) conceptualized the Army’s Integrated Training Area Management (ITAM) program in response to the concerns expressed by military land managers and trainers over the declining condition of training and testing lands. ITAM has evolved to include land condition and trend analysis (LCTA), environmental awareness (EA), training requirements integration (TRI), and land rehabilitation and maintenance (LRAM). The need for LRAM has increased dramatically over the past decade. Among the many difficulties faced in planning and implementing a land rehabilitation …

Land Rehabilitation And Maintenance Equipment Sources, Heidi Howard, Shannon Mcdowell, Amanda Breon, John A. Guretzky, Bradley Tadlock

Department of Agronomy and Horticulture: Faculty Publications

Military training can often be destructive to the environment. Environmental impacts associated with heavy training include soil compaction, soil erosion, siltation of waterways and wetlands, increased threat of flooding, loss of wildlife habitat, declining biodiversity, and invasion by noxious weeds. Planning and implementing land rehabilitation projects generally involves some soil movement, establishment of vegetation, stabilization and hardening of sites, etc. Procurement of appropriate heavy equipment and other specialized equipment is sometimes a difficult task that involves obtaining multiple vendors, especially when procuring equipment for rental, lease, or purchase. This report and associated database were created to help land managers easily …

C-Terminal 23 Kda Polypeptide Of Soybean Gly M Bd 28 K Is A Potential Allergen, Ping Xiang, Eric J. Haas, Michael G. Zeece, John P. Markwell, Gautam Sarath

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Gly m Bd 28 K is a major soybean (Glycine max Merr.) glycoprotein allergen. It was originally identified as a 28 kDa polypeptide in soybean seed flour. However, the full-length protein is encoded by an open reading frame (ORF) of 473 amino acids, and contains a 23 kDa C-terminal polypeptide of as yet unknown allergenic and structural characteristics. IgE-binding (allergenic potential) of the Gly m Bd 28 K protein including the 23 kDa C-terminal portion as well as shorter fragments derived from the full-length ORF were evaluated using sera from soy-sensitive adults. All of these sera contained IgE that …

Nebraska Vine Lines, Vol. Viii, No. 6. November/December 2004

Viticulture Publications and Research

• Fall Workshop: Instrumentation for Vineyards and Wineries – 95 attend.

• Edward Hellman

• The Grand Banquet to feature “Ragtime”

• Alcohol and Tobacco Tax and Trade Bureau to Speak at the March Forum in Kearney, NE

• Last Chance Winery

• Pioneer Nebraska Winery Turns “10”

• Taste Buds

• Masters Students to speak at 7th Forum in Kearney

Shiga Toxin Binding To Isolated Porcine Tissues And Peripheral Blood Leukocytes, Kellie R. K. Winter, William C. Stoffregen, Evelyn A. Dean-Nystrom

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Shiga toxin (Stx) binding sites in porcine tissues and leukocytes were identified by the use of Stx overlay and anti-CD77/Gb3 immunoassays. Stx1 and Stx2 bound to similar tissue locations and leukocytes, although some differences were noted. Previously unreported Stx binding sites were identified in kidney tubules, intestinal lymphoid aggregates, sinusoidal liver cells, alveolar macrophages, and peripheral blood leukocytes.

Tandem Inverted Repeat System For Selection Of Effective Transgenic Rnai Strains In Chlamydomonas, Jennifer Rohr, Nandita Sarkar, Susan Belanger, Byeong-Ryool Jeong, Heriberto D. Cerutti

Center for Plant Science Innovation: Faculty and Staff Publications

RNA interference (RNAi), the double-stranded RNA (dsRNA) triggered post-transcriptional gene silencing, is becoming a powerful tool for reverse genetics studies. Stable RNAi, induced by the expression of inverted repeat (IR) transgenes, has been achieved in protozoa, algae, fungi, plants, and metazoans. However, the level of gene silencing is often quite variable, depending on the type of construct, transgene copy number, site of integration, and target gene. This is a hindrance in functional genomics studies, where it is desirable to suppress target genes reliably to analyze unknown phenotypes. Consequently, we explored strategies for direct selection of effective transgenic RNAi lines in …

Interactions Among Western Ragweed And Other Sandhills Species After Drought, Patrick E. Reece, Joe E. Brummer, Brian K. Northup, Ann E. Koehler, Lowell E. Moser

Department of Agronomy and Horticulture: Faculty Publications

Interannual differences in yield and species composition of herbaceous vegetation on semiarid rangelands are common and often related to variations in precipitation regime. Interspecific interactions that occur after drought-induced population fluxes of western ragweed (Ambrosia psilostachya D.C.) were evaluated by removing western ragweed or associated species from 1-m² quadrats at weekly intervals beginning in early May, June, or July 1991 or 1992 on high-seral sandhills prairie in Nebraska. The composite of peak standing crops for ragweed and each group of associated species was 77% greater during May–October 1991 (2 252 kg•ha^-1) compared with 1992 (1 275 …

Nebline, November/December 2004

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Local Efforts to Promote Good Health
What Parents Can Do
Tax Planning Update for 2004
Don’t Forget to Winterize Your Underground Sprinkler System
Management Pays Better Than Labor
Management = Planning for the Future
Helping Trees Through Winter
Sources of Man-Made Chemicals in Drinking Water
How to Hire an Arborist
Tips on Storing Firewood
Tips on Turkey Preparation
WINTER FRUIT GLAZE Recipe
How to Cook a Turkey the Day Before Serving It
GREEN ONION AND CORN BREAD STUFFING Recipe
Simple Tip to Avoid Scratches When Storing Nonstick Skillets
Cooking Tools Class Oct. 26
President’s Notes — Janet’s Jargon
Household Hints: …

Temporal Origin Of Nitrogen In The Grain Of Irrigated Rice In The Dry Season: The Outcome Of Uptake, Cycling, Senescence And Competition Studied Using A 15N-Point Placement Technique, J. E. Sheehy, M. Mnzava, Kenneth G. Cassman, P. L. Mitchell, P. Pablico, R. P. Robles, H. P. Samonte, J. S. Lales, A. B. Ferrer

Department of Agronomy and Horticulture: Faculty Publications

It is often suggested that nitrogen absorbed in the vegetative stage of growth acts as a “reservoir” to supply the shortfall in demand during grain filling. The main objective of the work described in this paper was to investigate how effectively nitrogen absorbed at different stages of the growing season was retained and used for grain growth. The total nitrogen in the grain is the integral of the product of the total nitrogen absorbed at any instant and the eventual allocation of a fraction of that nitrogen to the grain. A point-placement technique was used to deliver small amounts of …

Necrotic Lesion Resistance Induced By Peronospora Tabacina On Leaves Of Nicotiana Obtusifolia, E.P. Heist, D. Zaitlin, Deanna L. Funnell, W.C. Nesmith, C.L. Schardl

Department of Plant Pathology: Faculty Publications

Infection of Nicotiana obtusifolia plant introduction (PI) #555573 by the downy mildew pathogen Peronospora tabacina resulted in a compatible interaction, in which P. tabacina penetrated and freely colonized host leaf tissue. This interaction became incompatible 5 to 6 days later, with the appearance of necrotic lesions (NLs) and inhibition of pathogen growth and subsequent sporulation. NL development depended upon the presence of P. tabacina in host tissue, was not due to the effects of other microbes, and occurred co-incident in time with the pathogen’s ability to produce asexual sporangia on a susceptible N. obtusifolia genotype. Inhibition of the necrotic response …

Chemical Composition That Attract Arthropods- Patent 2004, Ulrich R. Bernier, Donald R. Barnard, Matthew Booth, Daniel Kline, Kenneth H. Posey, Richard A. Yost

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Compositions and methods employing the compositions for attracting arthropods. The compositions comprise at least one compound of formula I and at least one compound from group II.

Long Polar Fimbriae Contribute To Colonization By Escherichia Coli O157:H7 In Vivo, Dianna M. Jordan, Nancy Cornick, Alfredo G. Torres, Evelyn A. Dean-Nystrom, James B. Kaper, Harley W. Moon

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The contribution of long polar fimbriae to intestinal colonization by Escherichia coli O157:H7 was evaluated in sheep, conventional pigs, and gnotobiotic piglets. E. coli O157:H7 strains with lpfA1 and lpfA2 mutated were recovered in significantly lower numbers and caused fewer attachment and effacement lesions than the parent strain.

Center For Grassland Studies Newsletter, Fall 2004, Volume 10, No. 4

Center for Grassland Studies: Newsletters

Nebraska's Natural Legacy Project by Kristal Stoner, Nebraska Game and Parks Commission

Earth, Wind, and Fire in the Flint Hills by David Hartnett, Konza Prairie Biological Station, Kansas State University

Owens Highlights UNL Grazing Activities at Grazing Conference

Professional Golf Management Program Tees Off with 31 Students

CGS Director Massengale Inducted into CSREES Hall of Fame

Development Of An Expressed Sequence Tag (Est) Resource For Wheat (Triticum Aestivum L.): Est Generation, Unigene Analysis, Probe Selection And Bioinformatics For A 16,000-Locus Bin-Delineated Map, G. R. Lazo, S. Chao, D. D. Hummel, H. Edwards, C. C. Crossman, N. Lui, D. E. Matthews, V. L. Carollo, D. L. Hane, F. M. You, G. E. Butler, R. E. Miller, T. J. Close, J. H. Peng, N. L. V. Lapitan, J. P. Gustafson, L. L. Qi, B. Echalier, B. S. Gill, M. Dilbirligi, H. S. Randhawa, K. S. Gill, R. A. Greene, M. E. Sorrells, E. D. Akhunov, J. Dvorak, A. M. Linkiewicz, J. Dubcovsky, K. G. Hossain, V. Kalavacharla, S. F. Kianian, A. A. Mahmoud, Miftahudin, X.-F. Ma, E. J. Conley, J. A. Anderson, M. S. Pathan, H. T. Nguyen, P. E. Mcguire, C. O. Qualset, O. D. Anderson

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

This report describes the rationale, approaches, organization, and resource development leading to a large-scale deletion bin map of the hexaploid (2n = 6x = 42) wheat genome (Triticum aestivum L.). Accompanying reports in this issue detail results from chromosome bin-mapping of expressed sequence tags (ESTs) representing genes onto the seven homoeologous chromosome groups and a global analysis of the entire mapped wheat EST data set. Among the resources developed were the first extensive public wheat EST collection (113,220 ESTs). Described are protocols for sequencing, sequence processing, EST nomenclature, and the assembly of ESTs into contigs. These contigs plus singletons …

Nebline, October 2004

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Discover 4-H, Discover You!
Five Ways to Join 4-H!
Nebraska Wild Fruit and Nut Jam Oct. 2
Ten Rules for Planting Trees
Garden Guide: Things to do this month
Direct Seeding Hardwood Tree Species
Storing Sweet Potatoes
Watch for Deer When Driving, Especially at Dawn and Dusk
Head Lice Resources You Can Trust
Nebraska Water Facts
The Hazards of Biting Fleas
Upcoming Household Hazardous Waste Collections
The Hazards of Biting Fleas
Prepare Bins and Equipment Before Harvest
“Grapes” is October Rural Living Clinic
Add Lime This Fall for Next Spring’s Alfalfa
Finding Forgotten Food
National Popcorn Poppin’ Month
Camp Abbott: …

A Single Amino Acid Substitution In Soybean Vspα Increases Its Acid Phosphatase Activity Nearly 20-Fold, Oranuch Leelapon, Gautam Sarath, Paul E. Staswick

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Soybean [Glycine max (L.) Merr.] contains two proteins called vegetative storage proteins (VSPs) that function as temporary storage reserves, but are also closely related to plant acid phosphatases of the haloacid dehalogenase (HAD) superfamily. This study examined the biochemical basis for the relatively low catalytic activity previously reported for these VSPs. The specific activity of purified recombinant VSPα on GMP was about 40-fold lower than for a related soybean root nodule acid phosphatase (APase), which had a specific activity of 845 U mg^-1 protein. Conversion of Ser¹⁰⁶ to Asp increased VSPα activity about 20-fold. This Asp residue …

Pedogenic Factors Affecting Magnetic Susceptibility Of The Last Interglacial Palaeosol S1 In The Chinese Loess Plateau, Z.-D. Feng, H. B. Wang, C. G. Olson

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The magnetic susceptibility has been used as a quantitative or semi-quantitative proxy for reconstructing the summer monsoon intensity in the Chinese Loess Plateau based on extensive studies on climatic or/and environmental mechanisms producing the magnetic susceptibility signatures. However, the precise nature of the link between past climates and the susceptibility signatures has remained uncertain primarily due to lack of our understanding in the finalizing and preserving processes of the signatures. This paper attempts to examine the reliability or acceptability of this summer monsoon proxy from non-magnetic perspectives of soil-forming processes. We chose nine sections along two transects: one across the …

Putting Genes Into Genetic Coefficients, P. Stephen Baenziger, Gregory S. Mcmaster, Wallace Wilhelm, Albert Weiss, Cynthia J. Hays

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Plant parameters are critical inputs in crop simulation models and allow a general set of algorithms to represent features of specific cultivars. A subset of plant parameters is often referred to as ‘‘genetic coefficients’’. However, these genetic coefficients are developed from phenotypic observations, usually have a weak genetic basis, and are at best ‘‘genotypic’’ coefficients because they consider the genotype from a very integrative perspective and likely include some impact of environment on the trait or characteristic described. With increased understanding of crop genomes, we believe models can be improved by incorporating genetic coefficients that accurately describe the action of …

Channel Aggradation By Beaver Dams On A Small Agricultural Stream In Eastern Nebraska, M. C. Mccullough, J. L. Harper, D. E. Eisenhauer, M. G. Dosskey

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

agricultural area of eastern Nebraska. A topographic survey was conducted of a reach of Little Muddy Creek where beaver are known to have been building dams for twelve years. Results indicate that over this time period the thalweg elevation has aggraded an average of 0.65 m by trapping 1730 t of sediment in the pools behind dams. Beaver may provide a feasible solution to channel degradation problems in this region.

Nebraska Vine Lines, Vol. Vii, No. 5, September/October 2004.

Viticulture Publications and Research

Contents

• Fall Workshop: Instrumentation for Vineyards and Wineries

• Electronic Nose Technologies: A New Instrumentation Option for Wineries?

• Just Getting Started – or “So You Want to Grow Grapes”

• Fall Vineyard Management By Paul Read, Viticulture Professor

• FUNDAMENTALS OF WINE EVALUATION

• Summer Closes with a Bang

Folate Biofortification In Tomatoes By Engineering The Pteridine Branch Of Folate Synthesis, Rocio Diaz De La Garza, Eoin P. Quinlivan, Sebastian M. J. Klaus, Gilles J. C. Basset, Jesse F. Gregory Iii, Andrew D. Hanson

Center for Plant Science Innovation: Faculty and Staff Publications

Plants are the main source of folate in human diets, but many fruits, tubers, and seeds are poor in this vitamin, and folate deficiency is a worldwide problem. Plants synthesize folate from pteridine, p-aminobenzoate (PABA), and glutamate moieties. Pteridine synthesis capacity is known to drop in ripening tomato fruit; therefore, we countered this decline by fruit-specific overexpression of GTP cyclohydrolase I, the first enzyme of pteridine synthesis. We used a synthetic gene based on mammalian GTP cyclohydrolase I, because this enzyme is predicted to escape feedback control in planta. This engineering maneuver raised fruit pteridine content by 3- …

The Prairie Naturalist Volume 36, No.3 September 2004

The Prairie Naturalist

OVERLAND MOVEMENTS AND HABITAT USE OF MALLARD BROODS DEPARTING OVERWATER NESTING STRUCTURES ▪ J. D. Stafford, L. D. Flake, and P. W. Mammenga

NEST SITE CHARACTERISTICS OF EASTERN WILD TURKEY IN NORTHEASTERN SOUTH DAKOTA ▪ R. D. Shields and L. D. Flake

WHITE-TAILED DEER INFECTEUWITH STAPHYLOCOCCUS HYICUS IN SOUTH DAKOTA ▪ T. J. Zimmerman, J. A. Jenks, and A. E. Pillatzki

CLOSTRIDIUM PERFRINGENS TYPE A IN A FREE-RANGING FAWN? ▪ T. J. Brinkman, J. A. Jenks, C. S. DePerno, and B. S. Haroldson

BUFFLEHEAD BREEDING ACTIVITY IN SOUTH-CENTRAL NORTH DAKOTA ▪ G. A. Knutsen and J. C. King

POTENTIAL MULTIPLE …

Nebline, September 2004

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

What if You Could Access Extension’s Educational Resources at the Push of a Button?
Sorting Through Internet Clutter
What Users Are Saying
Garden Guide: Things to do this month
Good, Low-Maintenance Trees to Plant
Herbal Festival
Aster Yellows on Purple Coneflower
Ladybugs, Ladybugs, Fly Away Home! Please!
Composting Indoors with Worms
Upcoming Household Hazardous Waste Collections
Packed Lunch Pizzazz!
Sports Mix Recipe
Adding A Rainbow To Your Diet
President’s Notes — Janet’s Jargon
Household Hints: Chill Out with an Efficient Fridge
FCE News & Events
Stress Affects Adolescents Too
Plan Now for Holiday Spending
Values
St. Johnswort is Increasing in …

Genetic Correlation Of Ram Sexual Performance With Ewe Reproductive Traits Of Four Sheep Breeds, G. Snowder, J. N. Stellflug, L. Dale Van Vleck

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Rams express differences in sexual performance during the breeding season. Breeding rams with high sexual performance scores as measured during a sexual performance test can improve flock fertility. Whether selecting rams for high sexual performance score will indirectly improve ewe reproductive performance is not known. The objective of this study was to estimate the genetic correlation between sexual performance scores of rams and reproduction of ewes. Sexual performance scores of rams and reproductive performance (number of lambs born per ewe exposed and number of lambs weaned per ewe exposed) from four breeds were analyzed with univariate and bivariate animal models …

Disabling Surveillance: Bacterial Type Iii Secretion System Effectors That Suppress Innate Immunity, Avelina Espinosa, James R. Alfano

Center for Plant Science Innovation: Faculty and Staff Publications

Many Gram-negative bacterial pathogens of plants and animals are dependent on a type III protein secretion system (TTSS). TTSSs translocate effector proteins into host cells and are capable of modifying signal transduction pathways. The innate immune system of eukaryotes detects the presence of pathogens using specific pathogen recognition receptors (PRRs). Plant PRRs include the FLS2 receptor kinase and resistance proteins. Animal PRRs include Tolllike receptors and nucleotide-binding oligomerization domain proteins. PRRs initiate signal transduction pathways that include mitogen-activated protein kinase (MAPK) cascades that activate defencerelated transcription factors. This results in induction of proinflammatory cytokines in animals, and hallmarks of defence …