Digital Commons Network^™

Gibberellic Acid Promotes Seed Germination In Penstemon Digitalis Cv. Husker Red, Anderson Machado De Mello, Nereu Augusto Streck, Erin E. Blankenship, Ellen Paparozzi

Department of Agronomy and Horticulture: Faculty Publications

Penstemon seed often shows an inconsistent or a low germination percentage. Although most select cultivars are propagated by cuttings, for export to other countries, seed is preferred. Three experiments were conducted to determine if soaking seed in gibberellic acid (GA₃) would increase seed germination of Penstemon digitalis cv. Husker Red. GA₃ concentrations used were 0, 10, 50, 100, 200, and 500 mg∙L^–1 (first experiment); 0, 500, 1000, and 1500 mg∙L^–1 (second experiment); and 0, 500, and 1000 mg∙L^–1 (third experiment). The first and second experiments were conducted in a growth chamber, whereas the third …

Bulked Segregant Analysis Using The Goldengate Assay To Locate The Rpp3 Locus That Confers Resistance To Soybean Rust In Soybean, D. L. Hyten, James R. Smith, Reid D. Frederick, Mark L. Tucker, Qijian Song, P. B. Cregan

Department of Agronomy and Horticulture: Faculty Publications

Few resistance loci to soybean rust (SBR), caused by Phakopsora pachyrhizi Syd., have been genetically mapped and linked to molecular markers that can be used for marker assisted selection. New technologies are available for single nucleotide polymorphism (SNP) genotyping that can be used to rapidly map traits controlled by single loci such as resistance to SBR. Our objective was to demonstrate that the highthroughput SNP genotyping method known as the GoldenGate assay can be used to perform bulked segregant analysis (BSA) to fi nd candidate regions to facilitate effi cient mapping of a dominant resistant locus to SBR designated Rpp3 …

Limits To Maize Productivity In The Western Corn-Belt: A Simulation Analysis For Fully Irrigated And Rainfed Conditions, Patricio Grassini, Haishun Yang, Kenneth G. Cassman

Department of Agronomy and Horticulture: Faculty Publications

Unlike the Central and Eastern U.S. Corn-Belt where maize is grown almost entirely under rainfed conditions, maize in the Western Corn-Belt is produced under both irrigated (3.2 million ha) and rainfed (4.1 million ha) conditions. Simulation modeling, regression, and boundary-function analysis were used to assess constraints to maize productivity in the Western Corn-Belt. Aboveground biomass, grain yield, and water balance were simulated for fully irrigated and rainfed crops, using 20-year weather records from 18 locations in combination with actual soil, planting date, plant population, and hybrid-maturity data. Mean values of meteorological variables were estimated for three growth periods (pre- and …

Evaluation Of Glyphosate-Tolerant And Conventional Alfalfa Weed Control Systems During The First Year Of Establishment, Robert Wilson Jr., Paul Burgener

Department of Agronomy and Horticulture: Faculty Publications

A field trial was conducted for 3 yr (2005 through 2007) near Scottsbluff, NE, to examine weed control, crop safety, forage production, and economics of glyphosate-tolerant and conventional alfalfa establishment systems. Glyphosate applied to alfalfa at the unifoliate growth stage provided 67% weed control and was similar to imazamox applied at the two-trifoliate leaf stage. Delaying glyphosate application until alfalfa had reached the two-trifoliate growth stage improved weed control to 83%, and weed control was similar to imazamox plus 2,4-DB and imazethapyr plus 2,4-DB. Imazamox and imazethapyr caused minor crop injury, and the addition of bromoxynil or 2,4-DB to both …

Fluid Fertilizer’S Role In Sustaining Soils Used For Bio-Fuels Production, John L. Kovar, Douglas Karlen

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Project Objectives:

1)To evaluate several S sources for corn grown on low organic matter soils
2)To investigate nutrient dynamics in a comprehensive tillage, nutrient management, and residue removal study.

Sulfur Response: Site Characteristics

• Eroded side slopes

• Loam/silt loam (Typic Haplaquolls)

• Corn after soybean

• N fertilizer applied at planting + spoke-whee UAN (155 lb N/A)

• Corn (Fontanelle 4693 planted 5/6/08, 32K plants/A

S Fertilizer Treatments

Control

30 lb S/A; 13-33-0-15S; 2x3

30 lb S/A; 21-0-0-24S; 2x3

30 lb S/A; 12-0-0-26S; 2x0

Expression And Functional Characterization Of The Plant Antimicrobial Snakin-1 And Defensin Recombinant Proteins, Natalia Kovalskaya, Rosemarie W. Hammond

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

In this study, for the first time, functionally active, recombinant, cysteine-rich plant proteins snakin-1 (SN1) and defensin (PTH1) were expressed and purified using a prokaryotic expression system. The overall level of antimicrobial activities of SN1 and PTH1 produced in Escherichia coli was commensurate with that of the same proteins previously obtained from plant tissues. Both proteins exhibited strong antibacterial activity against the phytopathogenic bacterium Clavibacter michiganensis subsp. sepedonicus (50% inhibitory concentration (IC₅₀) 1.5–8 lM) and antifungal activity against the phytopathogenic fungi Colletotrichum coccoides and Botrytis cinerea (IC₅₀ 5–14 lM). Significantly weaker activity was observed against Pseudomonas syringae …

Natural Enemies Of The Coccinellidae: Parasites, Pathogens, And Parasitoids, E. W. Riddick, T. E. Cottrell, K. A. Kidd

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

We review aspects of the life histories of representative enemies of coccinellids (both entomophagous and phytophagous species) and expose both potential and real effects that they have on life parameters of their hosts. Lady beetles are attacked by a variety of natural enemies (bacteria, fungi, mites, nematodes, protozoa, wasps, flies). Few of these enemies have the ability to alter significantly the population dynamics of their hosts. This review should encourage further research to help define the role of natural enemies in the population dynamics of coccinellids. Ultimately, the conservation of beneficial lady beetles and the management of nuisance and pestiferous …

Coccinellidae As Predators Of Mites: Stethorini In Biological Control, David J. Biddinger, Donald D. Weber, Larry A. Hull

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The Stethorini are unique among the Coccinellidae in specializing on mites (principally Tetranychidae) as prey. Consisting of 90 species in two genera, Stethorus and Parasthethorus, the tribe is practically cosmopolitan. The Stethorini are found in a diverse range of habitats, including many agricultural systems such as pome and stone fruits, brambles, tree nuts, citrus, avocadoes, bananas, papaya, palms, tea, cassava, maize, strawberries, vegetables, and cotton, as well as ornamental plantings, grasslands, forests, and heathlands. Tetranychid mite outbreaks became common in many agricultural systems only after World War II, when widespread use of broad-spectrum insecticides increased. Stethorini were initially appreciated …

Enzymatic Quantification Of Phytate In Animal Manure, Zhongqi He, Heidi W. Waldrip, C. Wayne Honeycutt, M. Susan Erich, Zachary N. Senwo

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Phytate (inositol hexaphosphate) has been identified as a major organic phosphorus (P) form in soil, animal manure, and other environmental samples. Although a number of methods are available for quantitative isolation and determination of phytate, they are time-consuming and not amenable to routine analysis. We developed a simple, rapid method for enzymatic determination of phytate in animal manure. Animal manure was extracted by H₂O, 1M hydrochloric acid (HCl), 0.1M sodium acetate (NaOAc, pH5.0) with or without 0.05M ethylenediaminetetraacetate (EDTA), and 0.25M or 0.5M sodium hydroxide (NaOH)–0.05M EDTA. Extracts were diluted (1/10–1/150) and adjusted to pH5.0 in sodium acetate …

Demographics And Habitat Selection For The Western Meadowlark (Sturnella Neglecta) In The Nebraska Sandhills, Matthew D. Giovanni

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

The prairie ecosystems of the Great Plains region in North America have largely been replaced and fragmented with industrial agriculture and invasive herbaceous and woody plant species. The concurrent and large-scale suppression of wildfire and elimination of grazing by native ungulates may have further decreased the availability and quality of habitat for wildlife. Indeed, 2004 estimates indicate only 30% of historic grasslands in the Great Plains still exist, while the trends of decreased land area enrolled in the Conservation Reserve Program and increased land area in commercial agriculture indicate continued loss of habitat. This decrease in habitat availability continues to …

Protein-Protein Interactions Of Tandem Affinity Purified Protein Kinases From Rice, Jai S. Rohila, Mei Chen, Shuo Chen, Johann Chen, Ronald L. Cerny, Christopher Dardick, Patrick Canlas, Hiroaki Fujii, Michael Gribskov, Siddhartha Kanrar, Lucas Knoflicek, Becky Stevenson, Mingtang Xie, Xia Xu, Xianwu Zheng, Jing-Kang Zhu, Pamela Ronald, Michael E. Fromm

Center for Plant Science Innovation: Faculty and Staff Publications

Eighty-eight rice (Oryza sativa) cDNAs encoding rice leaf expressed protein kinases (PKs) were fused to a Tandem Affinity Purification tag (TAP-tag) and expressed in transgenic rice plants. The TAP-tagged PKs and interacting proteins were purified from the T1 progeny of the transgenic rice plants and identified by tandem mass spectrometry. Forty-five TAPtagged PKs were recovered in this study and thirteen of these were found to interact with other rice proteins with a high probability score. In vivo phosphorylated sites were found for three of the PKs. A comparison of the TAP-tagged data from a combined analysis of 129 …

Effectors, Effectors Et Encore Des Effectors: The Xiv International Congress On Molecular-Plant Microbe Interactions, Quebec, Jonathan D. Walton, Tyler J. Avis, James R. Alfano, Mark Gijzen, Pietro Spanu, Kim Hammond-Kosack, Federico Sánchez

Center for Plant Science Innovation: Faculty and Staff Publications

The IS-MPMI held its fourteenth meeting in Quebec City July 19-23, 2009. There were 956 attendees from 49 countries. Just over 50% of the participants were students or postdoctoral associates. Hani Antoun (Université Laval, Canada) served as Program Chair, capably assisted by the Local Organizing Committee and the IS-MPMI International Advisory Board. There were eight plenary sessions, 18 concurrent sessions, and over 700 posters.

Fungal Physiology: A Future Perspective, Richard A. Wilson, Nicholas J. Talbot

Fungal Molecular Plant-Microbe Interactions

The study of fungal physiology is set to change dramatically in the next few years as highly scalable technologies are deployed allowing accurate measurement and identification of metabolites, proteins and transcripts within cells. The advent of next-generation DNA-sequencing technologies will also provide genome sequence information from large numbers of industrially relevant and pathogenic fungal species, and allow comparative genome analysis between strains and populations of fungi. When coupled with advances in gene functional analysis, protein-protein interaction studies, live cell imaging and mathematical modelling, this promises a step-change in our understanding of how fungal cells operate as integrated dynamic living systems

Nebraska Seed Focus September 2009, Nebraska Crop Improvement Association

Nebraska Seed Focus

Planning Ahead

Certified Seed Bags

Labeling Requirements for Treatment of Wheat Seed

AOSCA Elects Leadership

NCIA Membership Meeting!

Bulk Seed Sales

2009 Fall Seed Guides

Inspected Acres for 2009

Nebraska Seed Focus June 2009, Nebraska Crop Improvement Association

Nebraska Seed Focus

Seed Lab Remodeling

Certified Seed Bags

Congratulations

Carryover and New Crop Testing

Invitation to NCIA Membership

2009 Nebraska Winter Wheat Field Days

The Effect Of Adjuvants, Pesticide Formulation, And Spray Nozzle Tips On Spray Droplet Size, Robert N. Klein, Jeffrey A. Golus, Kelli L. Nelms

West Central Research and Extension Center, North Platte

Many factors, including adjuvants, pesticide formulations, and nozzle tips, affect spray droplet size. It is important to understand these factors as spray droplet size affects both drift and efficacy of pesticides, which is a main concern with pesticide application. A laser particle analyzer was used to determine the spray droplet size and distributions of a range of formulations sprayed through several types of nozzle tips. Nozzles included were extended range flat fan sizes 11003 and 11005 (Spraying Systems XR), air induction flat fan sizes 11005 and 11004 (AI) air induction extended range flat fan size 11005 (AIXR), preorifice flat fan …

Effect Of Drought And Agriculture On Ring-Necked Pheasant Abundance, Nebraska Panhandle, Charles J. Randel Iii

The Prairie Naturalist

The objectives of my study were to detennine the effects of drought (e.g., Palmer Modified Drought Severity Index; PMDI, Bridges et at. 2001) and/or agricultural practices (e.g., conversion) on RNP abundance in the Nebraska Panhandle (NP).

My RNP survey data were found to be correlated negatively to drought condition (PMDI) in January, February, and April. This was supported by Snyder (1984) and Riley (1995), both of whom reported that decreased precipitation in spring affected RNP production in the western Great Plains and Iowa, respectively. Late winter and early spring drought affect subsoil moisture and decrease primary production (Kiesselbach et al. …

The Prairie Naturalist Manuscript Submission Guidelines, Christopher N. Jacques, Troy W. Grovenburg, Jonathan Jenks

The Prairie Naturalist

These guidelines present The Prairie Naturalist (PNAT) policies and procedures for submitting scientific manuscripts for consideration for publication. In January 2009, a change in Editorial staff occurred and these guidelines address the ongoing transition and update the online "Suggestions for Contributors" guidelines provided on the PNAT website (http://www.fhsu.edu/biology/pn/prairienat.htm); these instructions supersede all previous guidelines. Tables and appendices are included for common word expressions with superfluous wording, examples of correct format and style guidelines for tables accompanying manuscripts, guidance in properly preparing Research Articles and Notes, citing literature, and mandatory abbreviations for tables, figures and parenthetical expressions.

Third Report Of The North Dakota Bird Records Committee: 2004-2005, Dan Svingen, Ron E. Martin

The Prairie Naturalist

Since 1979, North Dakota's bird records committee has collected, evaluated, and archived documentations of rare bird occurrences in the state. In 2004 and 2005, this committee resolved 189 rare bird records. On the basis of these record reviews, six species were ad~ed to the North Dakota state bird list: mottled duck (Anas fulvigula), yellow-billed loon (Cavia adamsii), white-tailed kite (Elanus leucurus), little stint (Calidris minuta), glaucous-winged gull (Larus glaucescens), and great black-backed gull (Larus marinus). Four species were added to the list of nesting species within the state: snowy …

Common Raven Nests In North Dakota After 100-Year Hiatus, Melvin P. Nenneman, Todd A. Grant, Ron E. Martin

The Prairie Naturalist

Our observations represent the first documented nesting by the common raven in North Dakota since the late 1800's. Houston (1977) suggested that the expansion of the American crow onto the Canadian prairies was limited by the scarcity of trees for nest sites, which also might have limited the extent of the common raven. Aspen woodland has increased substantially in and around J. Clark Salyer National Wildlife Refuge since European settlement, due primarily to fire suppression and extirpation of large herbivores (Grant and Murphy 2005). Thus, plausibly these increases in woodland habitat might be providing nest sites necessary for the common …

Factors Associated With Duck Use Of Impounded And Natural Wetlands In Western South Dakota, Jane E. Austin, Deborah A. Buhl

The Prairie Naturalist

Many wetlands in the northern Great Plains west of the Missouri River are stock ponds, created by impoundment of natural drainages or excavation of existing wetlands to provide water for livestock or improve habitat for waterfowl. We evaluated factors influencing use of wetlands by breeding duck pairs and broods relative to modification, water regime, size, and hydrological location on United States Forest Service lands within the Grand River National Grassland in northern South Dakota (2003 and 2004). Responses for both indicated pairs and broods were related positively to wet area, total wetland area within 4 km, emergent edge cover, and …

Brood Parasitism In A North American Population Of White-Faced Ibis, Mark E. Clark

The Prairie Naturalist

In 2007 while monitoring reproductive success among various overwater nesting birds at J. Clark Salyer National Wildlife Refuge in North Dakota, I observed parasitism of a white-faced ibis nest by a cattle egret. I located a nest on 23 May 2007 that contained four white-faced ibis eggs and one cattle egret egg. The nest was located in the middle of a small colony of nesting white-faced ibis (approximately 35 pairs) and black-crowned night herons (approximately 30 pairs). On 3 June 2007 all of the eggs were still present in the nest, but two of the white-faced ibis eggs began hatching …

Nebline, January 2009

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Creating a Strong Family: Strong Families Have Six Common Characteristics
Ways to Spend Time With Your Family in January
Private Pesticide Applicator Certification Opportunities
Soybean Cyst Nematode Spreading in Nebraska: Free Soil Sampling Kits at Extension Offices
Mystery “Bites”: Unidentifiable Skin Problems Usually Have Non-Insect Causes
Raccoons in the City
2009 Wall Calendar
Beginning Beekeeping 2-Day Workshop
2009 All-America Rose Selections
Shopping for Plants and Seeds
Garden Guide: Things to Do This Month
Birds Need Water In Winter
Attract Winter Birds With Feeders
Top 10 Myths of Entrepreneurship
Winter Houseplant Care
Wishing You a Healthy H-A-P-P-Y N-E-W Y-E-A-R!
$tretch Your …

Antibiotic Use In Animal Production: Environmental Concerns, Teshome H. Regassa, Richard K. Koelsch, Charles S. Wortmann, Richard F. Randle, Akwasi A. Abunyewa

Department of Agronomy and Horticulture: Faculty Publications

Introduction

Antibiotic1 use in animal production has led to improved feed use efficiency and increased growth rates. In turn, these resulted in reduced food production costs and reduced excrement of manure nutrients which may cause pollution problems.

On the other hand, antibiotic use leads to antibiotic resistance and a possible reduction in effectiveness of treatment options for both animals and humans. This publication addresses these issues.

Disease-causing microorganisms2, including bacteria,are the most diverse and numerous organisms on earth. Diverse in their habitat, environmental adaptation, and mechanisms of reproduction, they have short generations with high multiplication rates. Such characteristics help develop …

Mapping And Confirmation Of A New Allele At Rpp1 From Soybean Pi 594538a Conferring Rb Lesion–Type Resistance To Soybean Rust, Nanda Chakraborty, Joe Curley, Reid D. Frederick, D. L. Hyten, Randall L. Nelson, Glen L. Hartman, Brian W. Diers

Department of Agronomy and Horticulture: Faculty Publications

Soybean rust, caused by Phakopsora pachyrhizi (H. Sydow & Sydow), is a destructive soybean [Glycine max (L.) Merr.] disease and identification of new resistance genes is essential for effective rust management. Our research objectives were to map and confirm the location of resistance gene(s) in PI 594538A using a population of 98 F_3:4 lines from across between PI 594538A (reddish-brown [RB] lesions) and the susceptible cultivar Loda (tan [TAN] lesions). The lines were inoculated with the P. pachyrhizi isolate ZM01-1 from Zimbabwe. The RB resistance in PI 594538A mapped on linkage group G as a single dominant gene. …

Alternative Methods For Wetland Restoration In The Rainwater Basin, Nebraska, Usa, Nancy Nicolai, James L. Stubbendieck

Department of Agronomy and Horticulture: Faculty Publications

South-central Nebraska is recognized as a focal point of the mid-continent migratory waterfowl flyway. Substantial wetland alterations led to a critical need for restoration. Managers have restored wetlands by scraping with heavy earthmoving equipment to remove excess organic material and near-monocultures of reed canarygrass (Phalaris arundinacea L.) but managers report high costs and topsoil perturbation. Moderate livestock grazing was tested to compare results with those achieved with mechanical techniques. Advantages of grazing could include low costs and less soil perturbation. During the 2006 and 2007 growing seasons, we compared cover of bare ground and open water and plant species …

Forage Yields From 2008-2009 Small Grains, Jagadeesh Mosali, John A. Guretzky, M. Saha, J. Baker, S. Norton

Department of Agronomy and Horticulture: Faculty Publications

Introduction

Livestock and forage production are the largest contributors to agricultural income in the primary service region of the Noble Foundation. The small grains variety testing program which includes oats, rye, triticale and wheat is designed to provide up-to-date performance information to producers in Oklahoma and Texas about varieties that are commercially and commonly available. In addition, the program provides a tool to evaluate and compare experimental breeding lines emerging from the Noble Foundation breeding program as well as other public and private breeding programs. The program is intended to furnish producers with supplemental information and to aid decision-making and …

Research Emphasis – Forage And Bioenergy Crop Management, John A. Guretzky

Department of Agronomy and Horticulture: Faculty Publications

Guretzky specializes in research on forage and bioenergy crop management that will assist agricultural producers and landowners in meeting their goals. The first area of research involves Drs. Jon Biermacher and Billy Cook for the evaluation of management practices of switchgrass in forage and bioenergy systems. Objectives include: 1.) assessing potential on-farm biomass yields and production economics of switchgrass; 2.) documenting effects of location, harvest system and nitrogen fertilizer rates on biomass yields, forage quality, stand persistence and nutrient removal rates; 3.) evaluating establishment and biomass production responses to phosphorus and potassium fertilization; 4.) determining how stocker cattle gains on …

Blue Fescue Overseeding Improves Performance Of Fairway Height Buffalograsses, Bekele G. Abeyo, Robert C. Shearman, Roch E. Gaussoin, Leonard A. Wit, Desalegn D. Serba, Ugur Bilgili

Department of Agronomy and Horticulture: Faculty Publications

Buffalograss [Buchloe dactyloides (Nutt.) Engelm.] use as a fairway turfgrass is limited in northern portions of its adaptation zone by its extended winter dormancy and tan coloration in early spring and late fall. Cool-season grasses mixed with buffalograss could enhance turfgrass appearance and performance in fall and early spring. Research was conducted near Mead, NE, with eight buffalograss genotypes maintained under fairway conditions to determine the effect of blue fescue (Festuca ovina L. var. glauca Lam.) overseeding rate on turfgrass performance. Interactions were nonsignificant in most cases so main effects are emphasized. Differences were observed between seeding rates and …

Non-Destructive Determination Of Maize Leaf And Canopy Chlorophyll Content, Veronica Ciganda, Anatoly A. Gitelson, James S. Schepers

Department of Agronomy and Horticulture: Faculty Publications

The objective of this study was to develop a rapid non-destructive technique to estimate total chlorophyll (Chl) content in a maize canopy using Chl content in a single leaf. The approach was (1) to calibrate and validate a reflectance-based nondestructive technique to estimate leaf Chl in maize; (2) to quantify the relative contribution of each leaf Chl to the total Chl in the canopy; and (3) to establish a relationship between leaf Chl content and total Chl in a maize canopy. The Red Edge Chlorophyll Index Cl_{red edge} = (R_NIR/R_{red edge})-1 based on …