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Remote Sensing To Detect The Movement Of Wheat Curl Mites Through The Spatial Spread Of Virus Symptoms, And Identification Of Thrips As Predators Of Wheat Curl Mites, Abby R. Stilwell

Department of Entomology: Dissertations, Theses, and Student Research

The wheat curl mite (WCM), Aceria tosichella Keifer, transmits three viruses to winter wheat: wheat streak mosaic virus, High Plains virus, and Triticum mosaic virus. This virus complex causes yellowing of the foliage and stunting of plants. WCMs disperse by wind, and an increased understanding of mite movement and subsequent virus spread is necessary in determining the risk of serious virus infections in winter wheat. These risk parameters will help growers make better decisions regarding WCM management. The objectives of this study were to evaluate the capabilities of remote sensing to identify virus infected plants and to establish the potential …

Education In Plant Pathology Present Status And Future Challenges, James Macdonald, Caitilyn Allen, David Gadoury, William Jacobi, Segenet Kelemu, James Moyer, Tim Murray, Kevin Ong, Charles Pearson, John Sherwood, Ann Vidaver

Department of Plant Pathology: Faculty Publications

Plant pathology is largely a mission driven discipline that seeks to increase the fundamental understanding of host–pathogen interactions and the etiology of plant diseases for the purpose of preventing or mitigating crop loss. This is an important mission because all nations face serious challenges in their efforts to prevent disease on plants cultivated for food, fiber, ornamental use, timber, and fuel, as well as those growing in native ecosystems. In 1994, it was estimated that global losses due to plant diseases ranged from 9.7 to 14.2% of potential yield (12).

Observations Of Badgers Preying On Black-Tailed Prairie Dogs, Daniel S. Licht

The Prairie Naturalist

American badgers (Taxidea taxus) often visit black-tailed prairie dog (Cynomys ludovicianus) colonies, most likely in search of prey (Lomolino and Smith 2004, Shaughnessy and Cifelli 2004). Badgers are well suited to hunting fossorial prey such as prairie dogs by excavating burrows and capturing individuals belowground (Lindzey 2003). However, the ecological literature is sparse regarding details of how badgers hunt and capture prairie dogs underground. Eads and Biggins (2008) documented three occurrences of a badger excavating prairie dogs. That badger (apparently the same individual) had a den within the prairie dog town where captures occurred. This note …

Mortality Of Cranes (Gruidae) Associated With Powerlines Over A Major Roost On The Platte River, Nebraska, Gregory D. Wright, Timothy J. Smith, Robert K. Murphy, Jeffery T. Runge, Robert R. Harms

The Prairie Naturalist

Two 69-kilovolt powerlines spanning the Platte River in south central Nebraska are suspected to cause substantial mortality to sandhill cranes (Grus canadensis) and pose a threat to endangered whooping cranes (G. americana) that roost overnight on the river during spring and fall migrations. Most studies of crane collisions with powerlines in the region have focused on counts of carcasses away from night roosts on the river and none have accounted for potential biases in detecting carcasses. We found 61 carcasses of sandhill cranes below over-river segments of the two powerlines during 4 March to 7 April …

Long Distance Molt Migration By A Giant Canada Goose From Eastern South Dakota, Bobby J. Anderson, Charles D. Dieter

The Prairie Naturalist

To reduce crop damage by resident giant Canada geese (Schaible et al. 2005), the South Dakota Department of Game, Fish and Parks (SDGFP) initiated a program to reduce goose nesting success in eastern South Dakota. One management tool used by SDGFP personnel was the destruction of giant Canada goose nests. When a giant Canada goose has its nest destroyed, they are known to initiate a molt migration (Mykut 2002, Luukkonen et aI. 2008). We attached Platform Transmitting Terminals (PTT; model ST -19) to document and describe molt migrations of giant Canada geese following nest destruction. We captured 3 adult nesting …

Noteworthy Winter Prey Of Short-Eared Owls In Southern Texas: A Case Study, Damon Williford, Marc C. Woodin, Mary Kay Skoruppa

The Prairie Naturalist

The winter range of North American short-eared owls (Asio flammeus) encompasses much of the United States, including southern Texas, where it is a common winter resident (Oberholser 1974, Rappole and Blacklock 1985). Winter food habits of short-eared owls are relatively weJl documented, but the majority of investigations have been conducted in eastern Canada and northeastern U.S. (Clark 1975, Holt 1993); midwestern U.S. (Colvin and Spaulding 1983); and British Columbia and Pacific northwestern U.S. (Bogiatto et al. 2001). The short-eared owl has a narrow trophic niche, generally preying on small mammals, with voles (Microtus spp.) and deer mice …

Patterns Of Bison Hair Use In Nests Of Tallgrass Prairie Birds, Bryan R. Coppedge

The Prairie Naturalist

I examined patterns of bison (Bison bison L.) hair use by passerine birds nesting in the Tallgrass Prairie Preserve, Osage County, Oklahoma, 2002-2004. I collected and dissected 103 nests of 15 species into their constituent components. Nests were predominately composed of herbaceous material such as grass stems and leaves. Woody material and mud were rarely used. Bison hair was the most prominent zoological material used in nests, with lesser amounts and occurrence of arthropod silk, snake skin, feathers, jack rabbit (Lepus californicus Oray) fur, and man-made materials such as cellophane and string. At least one nest of 13 …

Monitoring Meadows With A Modified Robel Pole In The Northern Black Hills, South Dakota, Daniel W, Uresk, Daryl E. Mergen, Ted A. Benzon

The Prairie Naturalist

We used a modified Robel pole to measure vegetation for a study conducted in the northern Black Hills, South Dakota. Objectives were to determine the relationship between visual obstruction readings and clipped standing herbage, and develop guidelines for monitoring standing herbage. The relationship between visual obstruction readings and standing herbage was linear and regression coefficients were significant (P≤ 0.001). Herbage ranged from 140 to 3313 kg· ha-1 with a mean of 1386 kg· ha^-1 (SE = 320 kg· ha^-1) for 123 transects. Visual obstruction readings (VOR) ranged from 0.6 to 30.4 (number of 1.27 cm bands …

Bed Site Selection Of Fawn Pronghorn In Custer State Park, South Dakota, Chad P. Lehman, Jamin D. Hartland, Barbara J. Keller, Joshua J. Millspaugh, Gary C. Brundige

The Prairie Naturalist

We evaluated pronghorn (Antilocapra americana) fawn bedding site characteristics on a prairie and ponderosa pine (Pinus ponderosa) landscape interface in Custer State Park, South Dakota. We radio-marked 16 adult female pronghorn and collected bed site information from their fawns during 2007~2008. We compared bed site selection with random sites (n = 74) during 2 periods; the early hiding phase when fawns were 1 ~28 days of age (n = 23 bed sites) and the later group phase when fawns were 29~60 days of age (n = 52 bed sites). During the hiding phase fawns selected dry …

A Partial Inventory Of Islands In North Dakota: Potential For Breeding Waterfowl Management, Michael L. Szymanski

The Prairie Naturalist

Islands can provide secure nesting habitat for ducks and other waterbirds, especially in agriculturally dominated landscapes. I inventoried natural and man-made islands in the portion of North Dakota covered by the Prairie Pothole Joint Venture (PPJV). I mapped 1,305 islands in this area; up to 46% of which could provide enhanced nest success with management (e.g., predator removal or establishment of brushy cover). Management of islands for breeding ducks may be an important method for achieving desired reproductive rates in the PP JV as substantial areas of perennial grass cover are lost from federal conservation programs, primarily the Conservation Reserve …

Detection And Measurement Of Water Stress In Vegetation Using Visible Spectrum Reflectance, Arthur Zygielbaum

Department of Geography: Dissertations, Theses, and Student Research

At any scale, from a single microbe to the planet that nurtures us, water defines our place in the universe. It provides the hydraulic forces needed to give plants structure, and the medium enabling photosynthesis, the basis for most life on Earth, to occur. Knowledge of plant water status is vital to understanding the state or condition of vegetation, information which is essential to disciplines as diverse as agriculture, geography, and climatology. Non-destructive and remote sensing of plant water status allows the gathering of such information across wide geographic extents and over long periods of time. Monitoring vegetation remotely requires …

Ribosomal Protein L10 Is Encoded In The Mitochondrial Genome Of Many Land Plants And Green Algae, Jeffrey P. Mower, Linda Bonen

Center for Plant Science Innovation: Faculty and Staff Publications

Background: The mitochondrial genomes of plants generally encode 30-40 identified protein-coding genes and a large number of lineage-specific ORFs. The lack of wide conservation for most ORFs suggests they are unlikely to be functional. However, an ORF, termed orf-bryo1, was recently found to be conserved among bryophytes suggesting that it might indeed encode a functional mitochondrial protein.

Results: From a broad survey of land plants, we have found that the orf-bryo1 gene is also conserved in the mitochondria of vascular plants and charophycean green algae. This gene is actively transcribed and RNA edited in many flowering plants. Comparative sequence …

Wheat (Triticum Aestivum) Nam Proteins Regulate The Translocation Of Iron, Zinc, And Nitrogen Compounds From Vegetative Tissues To Grain, Brian M. Waters, Cristobal Uauy, Jorge Dubcovsky, Michael A. Grusak

Department of Agronomy and Horticulture: Faculty Publications

The NAM-B1 gene is a NAC transcription factor that affects grain nutrient concentrations in wheat (Triticum aestivum). An RNAi line with reduced expression of NAM genes has lower grain protein, iron (Fe), and zinc (Zn) concentrations. To determine whether decreased remobilization, lower plant uptake, or decreased partitioning to grain are responsible for this phenotype, mineral dynamics were quantified in wheat tissues throughout grain development. Control and RNAi wheat were grown in potting mix and hydroponics. Mineral (Ca, Cu, Fe, K, Mg, Mn, P, S, and Zn) and nitrogen (N) contents of organs were determined at regular intervals to …

Prediction Of Antigenic Epitopes On Protein Surfaces By Consensus Scoring, Shide Liang, Dandan Zheng, Chi Zhang, Martin Zacharias

Center for Plant Science Innovation: Faculty and Staff Publications

Background: Prediction of antigenic epitopes on protein surfaces is important for vaccine design. Most existing epitope prediction methods focus on protein sequences to predict continuous epitopes linear in sequence. Only a few structure-based epitope prediction algorithms are available and they have not yet shown satisfying performance.
Results: We present a new antigen Epitope Prediction method, which uses ConsEnsus Scoring (EPCES) from six different scoring functions - residue epitope propensity, conservation score, sidechain energy score, contact number, surface planarity score, and secondary structure composition. Applied to unbounded antigen structures from an independent test set, EPCES was able to predict antigenic eptitopes …

The Majority Of The Type Iii Effector Inventory Of Pseudomonas Syringae Pv. Tomato Dc3000 Can Suppress Plant Immunity, Ming Guo, Fang Tian, Yashitola Wamboldt, James R. Alfano

Center for Plant Science Innovation: Faculty and Staff Publications

The Pseudomonas syringae type III protein secretion system (T3SS) and the type III effectors it injects into plant cells are required for plant pathogenicity and the ability to elicit a hypersensitive response (HR). The HR is a programmed cell death that is associated with effector-triggered immunity (ETI). A primary function of P. syringae type III effectors appears to be the suppression of ETI and pathogen-associated molecular pattern–triggered immunity (PTI), which is induced by conserved molecules on microorganisms. We reported that seven type III effectors from P. syringae pv. tomato DC3000 were capable of suppressing an HR induced by P. fluorescens …

The Prairie Naturalist. Volume 41, No. 3/4, September/December 2009, The Great Plains Natural Science Society

The Prairie Naturalist

THE PRAIRIE NATURALIST MANUSCRIPT SUBMISSION GUIDELINES. C. N. Jacques, T. W. Grovenburg, and J. A. Jenks

A PARTIAL INVENTORY OF ISLANDS IN NORTH DAKOTA: POTENTIAL FOR BREEDING WATERFOWL MANAGEMENT. M. L. Szymanski

BED SITE SELECTION OF FAWN PRONGHORN IN CUSTER STATE PARK, SOUTH DAKOTA. C. P. Lehman, J. D. Hartland, B. J. Keller, J. J. Millspaugh, and G. C. Brundige

PATTERNS OF BISON HAIR USE IN NESTS OF TALL GRASS PRAIRIE BIRDS B. R. Coppedge

MORTALITY OF CRANES (GRUIDAE) ASSOCIATED WITH POWERLINES OVER A MAJOR ROOST ON THE NORTH PLATTE RIVER, NEBRASKA. G. D. Wright, T. J. Smith, R. K. …

Biofuels: Cultivating Energy, Not Invasive Species, Invasive Species Advisory Committee

National Invasive Species Council

Biofuels: Cultivating Energy, not Invasive Species, approved by ISAC on August 11, 2009

ISSUE

To provide alternatives to petroleum-based energy, the United States government has mandated a greater proportion of plant-based biofuels be integrated into its energy portfolio. However, certain plant species being proposed for biofuel production in the United States are invasive species or are likely to escape cultivation and become invasive. United States Executive Order (EO) 131121 defines invasive species as “alien [non-native] species whose introduction does or is likely to cause economic or environmental harm or harm to human health” and states: “Each Federal agency whose actions …

Experiencia De Productoras Con El Uso De Harina De Sorgo: Por Años El Cultivo Del Sorgo Ha Sido Utilizado Para El Consumo Humano Y Animal, Roxana Ortiz, Eduardo Funes

INTSORMIL Impacts and Bulletins

En marzo del año pasado, la industria panifi cadora experimento los precios altos que sufrió la harina de trigo y en la búsqueda de nuevas alternativas algunos de ellos se interesaron en el uso de harina de sorgo para elaborar pan dulce y bebidas étnicas.

Es así que el Centro Nacional de Tecnología Agropecuaria y Forestal (CENTA) a través del Laboratorio de Alimentos impulsa esta tecnología. El interés de probar nuevas opciones llevo a muchas Panaderías, Asociaciones de Productoras, ONG, Industrias de Alimentos, Fundaciones y otras a capacitarse en el uso de esta harina.

Triticum Mosaic Virus: A Distinct Member Of The Family Potyviridae With An Unusually Long Leader Sequence, Satyanarayana Tatineni, Amy D. Ziems, Stephen N. Wegulo, Roy French

Department of Plant Pathology: Faculty Publications

The complete genome sequence of Triticum mosaic virus (TriMV), a member in the family Potyviridae, has been determined to be 10,266 nucleotides (nt) excluding the 3′ polyadenylated tail. The genome encodes a large polyprotein of 3,112 amino acids with the “hall-mark proteins” of potyviruses, including a small overlapping gene, PIPO, in the P3 cistron. The genome of TriMV has an unusually long 5′ nontranslated region of 739 nt with 12 translation initiation codons and three small open reading frames, which resemble those of the internal ribosome entry site containing 5′ leader sequences of the members of Picornaviridae. Pairwise …

Effect Of Growth Stage On The Relationship Between Tan Spot And Spot Blotch Severity And Yield In Winter Wheat, Stephen N. Wegulo, Julie A. Breathnach, P. Stephen Baenziger

Department of Plant Pathology: Faculty Publications

Foliar fungal diseases frequently cause significant economic losses in the hard red winter wheat production areas of the Great Plains of the United States. In 2007, field experiments were conducted in four environments in Nebraska, USA to determine the crop growth stage at which severity of tan spot and spot blotch was most strongly related to yield in winter wheat. Secondary objectives were to evaluate the efficacy of fungicides in controlling tan spot and spot blotch and to determine the effect of fun¬gicide application timing on disease intensity and yield. Disease severity assessed at Zadoks growth stage (ZGS) 60 (flower¬ing) …

Effects Of Nitrogen Supply On The Root Morphology Of Corn And Velvetleaf, Kimberly D. Bonifas, John L. Lindquist

Department of Agronomy and Horticulture: Faculty Publications

Root morphology will affect interplant competition for soil nutrients. Research was conducted to assess fine root fraction, mean root radius, specific root length, root length density, and nitrogen (N) uptake per unit fine root biomass of corn and velvetleaf over time and in response to nitrogen supply. Pots containing either corn or velvetleaf were embedded in the ground and received one of three N treatments. Plants were destructively sampled on 10 dates during each of two years and root subsamples analyzed using root scanning software. While corn root morphology was more responsive to N supply than velvetleaf, velvetleaf N uptake …

Switchgrass For Forage And Bioenergy: Ii. Effects Of P And K Fertilization, John A. Guretzky, Maru Kering, Jon Biermacher, Billy Cook

Department of Agronomy and Horticulture: Faculty Publications

Switchgrass (Panicum virgatum L.) has been targeted for cellulosic ethanol production. Our objective was to evaluate effects of P and K fertilization on switchgrass biomass yields. Experiments were established in switchgrass (cv. Alamo) fields planted at three Oklahoma locations in 2007. Interactions of N and P fertilizer rates were evaluated on two sites that had low soil test P (6 to 9 kg/ha). Interactions of N and K fertilizer rates and harvest system were evaluated on a site that had low soil test K (134 kg/ha). In the N and P rate experiments, biomass yields during establishment …

An Integrated Crop- And Soil-Based Strategy For Variable-Rate Nitrogen Management In Corn, Darrin F. Roberts

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Nitrogen (N) management in cereal crops has been the subject of considerable research and debate for several decades. Historic N management practices have contributed to low nitrogen use efficiency (NUE). Low NUE can be caused by such things as poor synchronization between soil N supply and crop demand, uniform application rates of fertilizer N to spatially variable landscapes, and failure to account for temporally variable influences on soil N supply and crop N need. Active canopy reflectance sensors and management zones (MZ) have been studied separately as possible plant- and soil-based N management tools to increase NUE. Recently, some have …

A Preliminary Analysis Of The Effects Of Hr 2454 On U.S. Agriculture, Economic Research Service

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

USDA performed a preliminary economic analysis of the impacts of House-passed climate legislation, HR 2454, on U.S. agriculture. The analysis assumes no technological change, no alteration of inputs in agriculture, and no increase in demand for bio-energy as a result of higher energy prices. Therefore, it overstates the impact of the climate legislation on agriculture costs in the short (2012-18), medium (2027-2033), and long-term (2042 to 2048). In USDA’s analysis, short-term costs remain low in part because of provisions in HR 2454 that reduce the impacts of the bill on fertilizer costs. In fact, the impact on net farm income …

An Icosahedral Algal Virus Has A Complex Unique Vertex Decorated By A Spike, Mickaël V. Cherrier, Victor A. Kostyuchenko, Chuan Xiao, Valorie D. Bowman, Anthony J. Battisti, Xiaodong Yan, Paul R. Chipman, Timothy S. Baker, James L. Van Etten, Michael G. Rossmann

Department of Plant Pathology: Faculty Publications

Paramecium bursaria Chlorella virus-1 is an icosahedrally shaped, 1,900-Å-diameter virus that infects unicellular eukaryotic green algae. A 5-fold symmetric, 3D reconstruction using cryoelectron microscopy images has now shown that the quasiicosahedral virus has a unique vertex, with a pocket on the inside and a spike structure on the outside of the capsid. The pocket might contain enzymes for use in the initial stages of infection. The unique vertex consists of virally coded proteins, some of which have been identified. Comparison of shape, size, and location of the spike with similar features in bacteriophages T4 and P22 suggests that the spike …

Functional Characterization Of The Arabidopsis Β-Ketoacyl-Coenzyme A Reductase Candidates Of The Fatty Acid Elongase, Frédéric Beaudoin, Xianzhong Wu, Fengling Li, Richard P. Haslam, Jennifer E. Markham, Huanquan Zheng, Johnathan A. Napier, Ljerka Kunst

Department of Biochemistry: Faculty Publications

In plants, very-long-chain fatty acids (VLCFAs; >18 carbon) are precursors of sphingolipids, triacylglycerols, cuticular waxes, and suberin. VLCFAs are synthesized by a multiprotein membrane-bound fatty acid elongation system that catalyzes four successive enzymatic reactions: condensation, reduction, dehydration, and a second reduction. A bioinformatics survey of the Arabidopsis (Arabidopsis thaliana) genome has revealed two sequences homologous to YBR159w encoding a Saccharomyces cerevisiae β-ketoacyl reductase (KCR), which catalyzes the first reduction during VLCFA elongation. Expression analyses showed that both AtKCR1 and AtKCR2 genes were transcribed in siliques, flowers, inflorescence stems, leaves, as well as developing embryos, but only AtKCR1 …

Quantification Of Bt-Endotoxin Exposure Pathways In Carabid Food Webs Across Multiple Transgenic Events, Julie A. Peterson, John J. Obrycki, James D. Harwood

West Central Research and Extension Center, North Platte

Despite the reported specificity of Bacillus thuringiensis proteins against target pests, a number of studies have indicated that the uptake of Bt-endotoxins from bioengineered crops could have negative effects on natural enemies. It is therefore essential to quantify exposure pathways in non-target arthropod food webs across multiple transgenic events. Adult ground beetles (Coleoptera: Carabidae) were collected from transgenic corn fields expressing lepidopteran-specific Cry1Ab, coleopteran-specific Cry3Bb1, and both Cry1Ab and Cry3Bb1 (stacked event), as well as a non-transgenic isoline. Carabid gut-contents were screened for Cry1Ab Bt-endotoxin using enzyme-linked immunosorbent assay. Significant numbers of carabids tested positive for Cry1Ab from the lepidopteran-specific …

Usan Sorgo Para Hacer Pan: El Centa Capacita A Panifi Cadores Para Usar Esa Materia Prima, Roxana Ortiz

INTSORMIL Impacts and Bulletins

En marzo del año pasado, la industria panifi cadora experimento los precios altos que sufrió la harina de trigo. En la búsqueda de nuevas alternativas, algunos productores se interesaron en el uso de harina de sorgo para elaborar pan dulce y bebidas étnicas.

Por esa razón, el Centro Nacional de Tecnología Agropecuaria y Forestal (CENTA), a través del laboratorio de alimentos, impulsa esta tecnología.

Promueven Ventajas Nutritivas Del Sorgo: La Harina De Este Cereal Es Utilizada Para Elaborar Diversos Productivos Alimenticios, Roxana Ortiz

INTSORMIL Impacts and Bulletins

Horchata, tiste, atoles saborizados, poleadas, pudín, harina de afrecho, pan francés y dulce son productos elaborados a base de sorgo o maicillo y son mezclados con otros ingredientes, técnica impulsada por especialistas del laboratorio de alimentos del Centro Nacional de Tecnología Agropecuaria y Forestal (CENTA).

El proceso consiste en usar la harina sola de sorgo (100%) y mezclarla con otros cereales, rostizando el grano para la elaboración de bebidas o molido en seco para el desarrollo de otros productos.

A Nonsense Mutation In A Cinnamyl Alcohol Dehydrogenase Gene Is Responsible For The Sorghum Brown Midrib6 Phenotype1[W][Oa], Scott E. Sattler, Aaron J. Saathoff, Eric J. Haas, Nathan A. Palmer, Deanna L. Funnell-Harris, Gautam Sarath, Jeffrey F. Pedersen

Department of Plant Pathology: Faculty Publications

brown midrib6 (bmr6) affects phenylpropanoid metabolism, resulting in reduced lignin concentrations and altered lignin composition in sorghum (Sorghum bicolor). Recently, bmr6 plants were shown to have limited cinnamyl alcohol dehydrogenase activity (CAD; EC 1.1.1.195), the enzyme that catalyzes the conversion of hydroxycinnamoyl aldehydes (monolignals) to monolignols. A candidate gene approach was taken to identify Bmr6. Two CAD genes (Sb02g024190 and Sb04g005950) were identified in the sorghum genome based on similarity to known CAD genes and through DNA sequencing a nonsense mutation was discovered in Sb04g005950 that results in a truncated protein lacking the NADPH-binding and C-terminal …