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Articles 61 - 90 of 929
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Solutions To Soil Problems: Iv. Soil Structure, Rich Koenig, Teresa Cerny
Solutions To Soil Problems: Iv. Soil Structure, Rich Koenig, Teresa Cerny
All Current Publications
Soil structure refers to the combination of primary soil particles – sand, silt and clay – into larger units called aggregates or clods. Aggregates are commonly seen when a soil is tilled or disturbed.
Utah Fertilizer Guide, D. W. James, K. F. Topper
Utah Fertilizer Guide, D. W. James, K. F. Topper
All Current Publications
The Utah Fertilizer Guide is designed to provide practical answers to routine questions related to soil fertility management. The guide emphasizes laboratory analysis of soil and plant samples as the keystone to optimum fertilizer rates for maximum economic plant yield and quality. The focus of the guide is on soil fertility problems peculiar to the semi-arid and arid soils of Utah.
Analysis Of The Response Of Medicago Truncatula Calcium Oxalate Mutants To Abiotic Stress, Wayra Gabriela Navia-Gine
Analysis Of The Response Of Medicago Truncatula Calcium Oxalate Mutants To Abiotic Stress, Wayra Gabriela Navia-Gine
Graduate Theses and Dissertations
Calcium oxalate crystals are found in most plant species. In Medicago truncatula wild-type A17, crystals accumulate in leaves along the secondary veins and the only role attributed to them so far is defense against chewing insects. Calcium oxalate deficient (cod) mutants were isolated in M. truncatula; the cod mutants include cod5, which completely lack crystals in the leaves and cod6 that accumulates fewer and smaller crystals compared to A17. We analyzed gene expression in the cod mutants and A17 using GeneChip® Medicago Genome Arrays and found important differences in transcriptome between the three genotypes. In particular, we found a gene …
Investigating Heavy Metal Accumulation And Oxidative Stress Tolerance Of Non-Accumulators And Hyperaccumulators In The Brassicaceae Plant Family, Fritzie Joy Into
Investigating Heavy Metal Accumulation And Oxidative Stress Tolerance Of Non-Accumulators And Hyperaccumulators In The Brassicaceae Plant Family, Fritzie Joy Into
Theses and Dissertations - UTB/UTPA
Physiological and enzymatic analysis indicated that the non-accumulator A. thaliana showed an oxidative stress response in all assays except for protein carbonylation. Other non-accumulator plant species showed a significant difference in oxidative stress response in the TBARS assay; however, for some assays no stress response was evident. The hyperaccumulator plant species showed no significant difference in oxidative stress as indicated by the all of the assays with the exception of T. montanum var. montanum which showed an oxidative stress response in the SOD assay. Basal catalase enzyme activity was notably higher in the hyperaccumulators T. montanum var. montanum and T. …
The Prairie Naturalist, Vol. 42, Issue 3/4, December 2010, The Great Plains Natural Science Society
The Prairie Naturalist, Vol. 42, Issue 3/4, December 2010, The Great Plains Natural Science Society
The Prairie Naturalist
EDITOR'S NOTE: REPORTING RESULTS OF DATA ANALYSIS, PREPARING SCIENTIFIC MANUSCRIPTS, AND WEBSITE DEVELOPMENT EFFORTS • Christopher N. Jacques
Current Distribution of Rare Fishes in Eastern Wyoming Prairie Streams • Christina E. Barrineau, Elizabeth A. Bear, and Anna C. Senecal
Diets of Nesting Swainson's Hawks in Relation to Land Cover in Northwestern North Dakota • Robert K. Murphy
Resource Selection of Greater Prairie-Chicken and and Sharp-Tailed Grouse Broods in Central South Dakota • Mark A. Norton, Kent C. Jensen, Anthony P. Leif, Thomas R. Kirschenmann, and Gregory A. Wolbrink
Population Characteristics of Central Stonerollers in Iowa Streams • Scott M Bisping, …
Cropland Nesting By Long-Billed Curlews In Southern Alberta, James H. Devries, Steven O. Rimer, Elizabeth M. Walsh
Cropland Nesting By Long-Billed Curlews In Southern Alberta, James H. Devries, Steven O. Rimer, Elizabeth M. Walsh
The Prairie Naturalist
Long-billed curlews (Numenius americanus) are described primarily as a grassland nesting species. However, no studies to date have quantified nest habitat selection among available habitats. During a study of waterfowl nest habitat selection and success in landscapes ranging from cropland to grassland-dominated, we found 9 curlew nests of which 8 were located in active cropland within cropland-dominated landscapes. Cropland nests occurred in fall-seeded winter wheat and spring-seeded barley and nests were clumped in distribution. Four cropland nests and 1 nest in native grass pasture hatched young. Further research is needed to characterize nesting habitat selection and reproductive success …
Examination Of Owl Pellets For Northern Pocket Gophers At Crescent Lake National Wildlife Refuge, Nebraska, Stacey L. Bonner, Keith Geluso
Examination Of Owl Pellets For Northern Pocket Gophers At Crescent Lake National Wildlife Refuge, Nebraska, Stacey L. Bonner, Keith Geluso
The Prairie Naturalist
Analysis of regurgitated pellets from owls is a well-known and nondestructive method that provides useful information regarding diet (Errington 1930). This technique also is used to examine composition of small mammal communities and distribution of prey species (e.g. Kamler et al. 2003, Torre et al. 2004, Poole and Matlack 2007). In western Nebraska, two species of owls that regularly breed in the region are the common barn owl (Tyto alba) and great horned owl (Bubo virginianus). In the early 1970s, Rickart (1972) studied the diet of both species at Crescent Lake National Wildlife Refuge (CLNWR), Garden …
Resource Selection Of Greater Prairie-Chicken And Sharp-Tailed Grouse Broods In Central South Dakota, Mark A. Norton, Kent C. Jensen, Anthony P. Leif, Thomas R. Kirschenmann, Gregaory A. Wolbrink
Resource Selection Of Greater Prairie-Chicken And Sharp-Tailed Grouse Broods In Central South Dakota, Mark A. Norton, Kent C. Jensen, Anthony P. Leif, Thomas R. Kirschenmann, Gregaory A. Wolbrink
The Prairie Naturalist
Habitat use of sympatric greater prairie-chicken (Tympanuchus cupido) and sharp-tailed grouse (T. phasianellus) broods during the brood-rearing season has not been quantified for stable prairie grouse populations in large contiguous grassland landscapes in the Northern Great Plains. Characteristics of habitats used by prairie grouse broods were described based on data collected from 35 broods (18 greater prairie-chicken and 17 sharp-tailed grottse) during the breeding seasons of 2004 and 2005. Greater prairie-chicken and sharp-tailed grouse broods used vegetation with visual obstruction heights 2:26 cm and 37 cm, respectively. Greater prairie-chicken broods selected western wheatgrass (Pascopyrum smithii …
Estimating Maize Grain Yield From Crop Biophysical Parameters Using Remote Sensing, Noemi Guindin-Garcia
Estimating Maize Grain Yield From Crop Biophysical Parameters Using Remote Sensing, Noemi Guindin-Garcia
Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research
The overall objective of this investigation was to develop a robust technique to predict maize (Zea mays L.) grain yield that could be applied at a regional level using remote sensing with or without a simple crop growth simulation model. This study evaluated capabilities and limitations of the Moderate Resolution Imaging Spectroradiometer (MODIS) Vegetation Index 250-m and MODIS surface reflectance 500-m products to track and retrieve information over maize fields. Results demonstrated the feasibility of using MODIS data to estimate maize green leaf area index (LAIg). Estimates of maize LAIg obtained from Wide Dynamic Range Vegetation …
The Impact Of Long-Term Tillage, Crop Rotation And N Application On Soil Carbon Sequestration, Agustin Martellotto
The Impact Of Long-Term Tillage, Crop Rotation And N Application On Soil Carbon Sequestration, Agustin Martellotto
Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research
Soil organic carbon (SOC) stock is controlled by many factors, but for given conditions it is the long term summation of the balance between inputs and outputs. Management practices will alter this balance by affecting the system’s productivity and the speed of residue and soil organic matter decomposition. Given that annual changes in SOC are generally small, compared with the large and variable SOC background, long-term experiments, and soil samples taken at the start of experiments (archived samples) are necessary to determine SOC trends over time. Changes in SOC were analyzed for two long-term experiments; one an irrigated site at …
Comparing Cropping System Productivity Between Fixed Rotations And A Flexible Fallow System Using Modeling And Historical Weather Data In The Semi-Arid Central Great Plains, Juan Jose Miceli-Garcia
Comparing Cropping System Productivity Between Fixed Rotations And A Flexible Fallow System Using Modeling And Historical Weather Data In The Semi-Arid Central Great Plains, Juan Jose Miceli-Garcia
Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research
In the Central Great Plains, the predominant crop rotation is winter wheat (Triticum aestivum L.)-fallow. Producers are looking to add diversity and intensity to their cropping systems by adding summer crops, however, the elimination of summer fallow may increase crop production risk. The objective of this study was to use crop simulation modeling to compare the productivity of two fixed rotations [winter wheat-corn (Zea mays L.)-fallow and winter wheat-corn-spring triticale (X Triticosecale Wittmack)] with simulated flexible fallow rotations. The flexible fallow rotations made the decision to plant triticale or use summer fallow prior to winter wheat seeding based …
Detection Of Soybean Seed Protein Qtls Using Selective Genotyping, Piyaporn Phansak
Detection Of Soybean Seed Protein Qtls Using Selective Genotyping, Piyaporn Phansak
Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research
A quantitative trait locus (QTL) is a statically defined location of a gene governing that trait. QTL identification is the first step towards using marker-assisted selection (MAS) to introgress desirable QTL alleles into elite high-yield cultivars. Hundreds of high protein plant introductions (PIs) exist in the USDA germplasm collection and are a source of high protein alleles. Although 86 protein QTLs are currently listed in SoyBase, many are likely repeat discoveries of the same QTL(s), given the typical +/- 10 cM confidence intervals associated with QTL positions. Six germplasm accessions of maturity groups (MGs) II to IV that exhibited high …
Micropropagation And Acclimatization Of 'Norton' Grapevine (Vitis Aestivalis), Brant B. Bigger
Micropropagation And Acclimatization Of 'Norton' Grapevine (Vitis Aestivalis), Brant B. Bigger
Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research
Propagation of the Vitis aestivalis cultivar 'Norton‘ (syn='Cynthiana‘) through traditional woody cuttings has been difficult. Rooting of woody cuttings has been a major hindrance in propagating this cultivar and providing enough plants to meet grower needs. In vitro propagation offers another method of increasing plant material. Cultures were established and maintained on Murashige and Skoog (MS) medium supplemented with 4 M 6-benzyladenine (BA) and thiamine at 0.5 mg•L-1 and solidified with Difco-Bacto agar at 7.5 g•L-1. The objectives of this study were to determine optimal methods for in vitro production and ex vitro establishment of 'Norton‘ plantlets. …
Evaluation Of Novel Input Output Traits In Sorghum Through Biotechnology, Tejinder K. Mall
Evaluation Of Novel Input Output Traits In Sorghum Through Biotechnology, Tejinder K. Mall
Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research
Sorghum [Sorghum bicolor (L.) Moench] is the fifth most important cereal crop world-wide as well as an important source of feed, fiber and biofuel. It is a C4 plant and is well adapted to environments subject to high temperature and water limitation. Despite these agronomic qualities, it suffers from limitations of sensitivity to low temperature and the grain has drawbacks in relation to functionality and digestibility. The objectives of this study are: 1. To promote seed germination at low temperature and to enhance the seedling cold tolerance. 2. To enhance the grain digestibility and functionality. In an attempt to …
Delaying Bud Break In ‘Edelweiss’ Grapevines To Avoid Spring Frost Injury By Naa And Vegetable Oil Applications, Issam M. Qrunfleh
Delaying Bud Break In ‘Edelweiss’ Grapevines To Avoid Spring Frost Injury By Naa And Vegetable Oil Applications, Issam M. Qrunfleh
Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research
Delaying bud break is an approach to avoid spring frost damage. Field experiments were conducted during the winters of 2009 and 2010 at James Arthur Vineyards in Raymond, Nebraska to study the effect of spraying NAA and Amigo Oil on delaying bud break in ‘Edelweiss’ grapevines to avoid such damage. In 2009, the experiment consisted of five treatments: NAA (500, 750, and 1000 mg/l), oil applied at 10%, and the non-sprayed control. There were four application dates: January 6, February 3, March 3, and April 1. Bud break was evaluated throughout spring. During harvest, the number of clusters and weights …
Farmers' Markets In Kentucky: A Geospatial, Statistical, And Cultural Analysis, Elizabeth Ann Schmitz
Farmers' Markets In Kentucky: A Geospatial, Statistical, And Cultural Analysis, Elizabeth Ann Schmitz
Masters Theses & Specialist Projects
To assess what factors are driving the exponential growth of farmers’ markets in Kentucky, geospatial and statistical analysis of a database of 121 farmers’ markets was conducted. A statewide survey of market leaders and a case study of a single farmers’ market both identified reasons for growing support of farmers’ markets in Kentucky.
Market distribution, vendor levels, and gross sales were mapped against a backdrop of county urban classification, median household income, and education levels. Kruskal-Wallace analysis was used to identify if Kentucky’s rural, micropolitan, and metropolitan markets differ significantly in terms of their age, number of vendors, and market …
Response Of Warm Season Turfgrasses To Reduced Light Environments, Jeffrey Atkinson
Response Of Warm Season Turfgrasses To Reduced Light Environments, Jeffrey Atkinson
All Theses
Shade or low light tolerance is an increasingly important issue to turf managers as they are often expected to grow turf in less than ideal agronomic conditions. As permanent structures such as residential buildings add to already problematic shade caused by trees, and other barriers, new solutions are needed to help turf managers provide acceptable turf conditions. The plant growth regulator trinexapac-ethyl (TE) can lessen negative responses of turfgrass to shade.
Two experiments were conducted during the summers of 2008 and 2009 to evaluate various grasses under a reduced light environment (RLE). In the first study, performance of `Diamond' zoysiagrass …
Solutions To Soil Problems: I. High Salinity (Soluble Salts), Vernon Parent, Rich Koenig
Solutions To Soil Problems: I. High Salinity (Soluble Salts), Vernon Parent, Rich Koenig
All Current Publications
Salinity is a measure of the total amount of soluble salts in soil. As soluble salt levels increase, it becomes more difficult for plants to extract water from soil. Some plants are more resistant than other’s, but as the salt levels exceed their ability to extract water, they become water stressed.
Selecting And Propagating Clones Of Bigtooth Maple (Acer Grandidentatum Nutt.), Melody Reed Richards
Selecting And Propagating Clones Of Bigtooth Maple (Acer Grandidentatum Nutt.), Melody Reed Richards
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
Numerous wild bigtooth maple (Acer grandidentatum Nutt.) specimens in northern Utah have potential for use in landscapes, but improvements in selection and propagation need to be developed before these specimens can be introduced to the green industry. Criteria-based evaluations centered on aesthetics, function, and fall color were performed to objectively select superior bigtooth maple specimens. Out of 56 trees initially selected for red fall color, six were selected for propagation based on all three criteria. Five of the six selected trees yielded viable bud take via chip budding. Optimum time for chip budding propagation was determined by four experiments. …
Enhancing Out-Of-Season Production Of Tomatoes And Lettuce Using High Tunnels, Britney L. Hunter
Enhancing Out-Of-Season Production Of Tomatoes And Lettuce Using High Tunnels, Britney L. Hunter
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
The growing season for vegetable crops is limited by freezing temperatures in arid high elevation climates such as northern Utah. Logan, Utah (41.73 N, 111.83 W, 1382 m elevation) has a short, variable growing season with an average frost-free period of 135 days. Extending the growing season provides growers with an opportunity to extend revenue into a normally unproductive period and benefit from out-of-season price premiums. High tunnels have been used to effectively extend the growing season for numerous crops by providing cold temperature protection. However, limited high tunnel research has been performed in arid high elevation regions that experience …
Phenotypic And Genetic Characterization Of Wildland Collections Of Western And Searls Prairie Clovers For Rangeland Revegetation In The Western Usa, Kishor Bhattarai
Phenotypic And Genetic Characterization Of Wildland Collections Of Western And Searls Prairie Clovers For Rangeland Revegetation In The Western Usa, Kishor Bhattarai
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
Western prairie clover [Dalea ornata (Douglas ex Hook.) Eaton & J. Wright] is a perennial legume that occurs in the northern Great Basin, Snake River Basin, and southern Columbia Plateau, whereas Searls prairie clover [Dalea searlsiae (A. Gray) Barneby], also a perennial legume, occurs in the southern Great Basin and surrounding areas. Understanding the genetic and ecotypic variation of these prairie clovers is a prerequisite for developing populations suitable for rangeland revegetation in the western USA. DNA sequences of internal transcribed spacer (ITS/5.8S) and trnK/matK were used to study the phylogeny of these species. The species were distinguished …
An Investigation Of The Utilization Of Soil Water And Nitrogen Among Diverse Forage Plant Species And Mixtures, Sallee Reynolds
An Investigation Of The Utilization Of Soil Water And Nitrogen Among Diverse Forage Plant Species And Mixtures, Sallee Reynolds
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
Species diversity achieved by adding novel functional groups (warm-season grasses and non-leguminous forbs) to pasture land, along with traditional grasses and legumes, could aid in the capture of nutrients and water in pasture systems by offering complementary rooting architecture to aid in water and nitrogen uptake and decrease nitrogen leaching. Because these species may differ from commonly used grasses and legumes in their seasonal pattern of productivity, they could also extend or enhance growing-season productivity. The goal of this project is to better understand the role of plant diversity in 1) nitrogen use and 2) distribution of rooting dynamics and …
Interclonal Variation Of Primary And Secondary Chemistry In Western Quaking Aspen And Its Influence On Ungulate Selection, Damon A. Winter
Interclonal Variation Of Primary And Secondary Chemistry In Western Quaking Aspen And Its Influence On Ungulate Selection, Damon A. Winter
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
Quaking aspen (Populus tremuloides) clones within close proximity to one another can exhibit drastically different levels of browsing by ungulates. The objectives of this study were to (1) determine interclonal differences in plant chemistry between adjacent clones exhibiting different degrees of herbivory which may influence the browsing behavior and patterns of ungulates, and (2) determine if correlation exists in the levels of salicortin and tremulacin between current year's suckers and current year's growth on older trees. This second objective was meant to indicate a protocol for land managers for identifying clones meriting increased protection from herbivory after treatment …
Using Mulches In Utah Landscapes And Gardens, Rich Koenig, Kitt Farrell-Poe Phd, Bruce Miller
Using Mulches In Utah Landscapes And Gardens, Rich Koenig, Kitt Farrell-Poe Phd, Bruce Miller
All Current Publications
This publication discusses the use of mulches in Utah landscapes including benefits and application information.
Solutions To Soil Problems: Ii. High Ph (Alkaline Soil), Loralie Cox, Rich Koenig
Solutions To Soil Problems: Ii. High Ph (Alkaline Soil), Loralie Cox, Rich Koenig
All Current Publications
PH is a measure of the acidity or alkalinity of a material. The pH scale ranges from 0 to 14, with 7 being neutral. A pH value below 7 indicates the soil is acidic, while values above 7 are alkaline. Each unit change in the pH scale is a 10-fold difference in acidity or alkalinity. For example, soil with a pH of 8 is ten times more alkaline than soil with a pH of 7.
Solutions To Soil Problems: V. Low Organic Matter, Kevin Heaton, Rich Koenig
Solutions To Soil Problems: V. Low Organic Matter, Kevin Heaton, Rich Koenig
All Current Publications
Utah soils are inherently low in organic matter due to the desert climate and historically low plant growth rates. In Utah, soil organic matter levels are typically 0.25 to 1%, while regions with high rainfall such as the Midwest and Eastern United States have soils with as much as 7 to 10% organic matter.
Population Variability Of Rotylenchulus Reniformis In Cotton Agroecosystems, Megan Leach
Population Variability Of Rotylenchulus Reniformis In Cotton Agroecosystems, Megan Leach
All Dissertations
Rotylenchulus reniformis, reniform nematode, is a highly variable species and an economically important pest in many cotton fields across the southeast. Rotation to resistant or poor host crops is a prescribed method for management of reniform nematode. An increase in the incidence and prevalence of the nematode in the United States has been reported over the last two decades. However it is not clear whether the observed increase is related to the emergence of novel populations that are more aggressive or have a higher fitness or shifts in host availability or susceptibility. The objectives of this research were to determine …
Substrates Of The Arabidopsis Thaliana Protein Isoaspartyl Methyltransferase 1 Identified Using Phage Display And Biopanning, Tingsu Chen, Nihar Nayak, Susmita Maitra Majee, Jonathan Lowenson, Kim R. Schäfermeyer, Alyssa C. Eliopoulos, Taylor D. Lloyd, Randy Dinkins, Sharyn E. Perry, Nancy R. Forsthoefel, Steven G. Clarke, Daniel M. Vernon, Zhaohui Sunny Zhou, Tomas Rejtar, A. Bruce Downie
Substrates Of The Arabidopsis Thaliana Protein Isoaspartyl Methyltransferase 1 Identified Using Phage Display And Biopanning, Tingsu Chen, Nihar Nayak, Susmita Maitra Majee, Jonathan Lowenson, Kim R. Schäfermeyer, Alyssa C. Eliopoulos, Taylor D. Lloyd, Randy Dinkins, Sharyn E. Perry, Nancy R. Forsthoefel, Steven G. Clarke, Daniel M. Vernon, Zhaohui Sunny Zhou, Tomas Rejtar, A. Bruce Downie
Horticulture Faculty Publications
The role of protein isoaspartyl methyltransferase (PIMT) in repairing a wide assortment of damaged proteins in a host of organisms has been inferred from the affinity of the enzyme for isoaspartyl residues in a plethora of amino acid contexts. The identification of PIMT target proteins in plant seeds, where the enzyme is highly active and proteome long-lived, has been hindered by large amounts of isoaspartate-containing storage proteins. Mature seed phage display libraries circumvented this problem. Inclusion of the PIMT co-substrate, S-adenosylmethionine (AdoMet), during panning permitted PIMT to retain aged phage in greater numbers than controls lacking co-substrate or when …
Fusarium Head Blight: Winter Wheat Cultivar Responses And Characterization Of Pathogen Isolates, John Fredy Hernandez Nopsa
Fusarium Head Blight: Winter Wheat Cultivar Responses And Characterization Of Pathogen Isolates, John Fredy Hernandez Nopsa
Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research
Fusarium head blight (FHB) is a destructive disease of wheat (Triticum aestivum L). FHB reduces yield and grain quality and causes accumulation of the mycotoxin deoxynivalenol (DON) in grain. Cultivar resistance is one of the most effective management strategies for FHB. Experiments were conducted to 1) identify winter wheat cultivars with resistance to FHB and DON accumulation, 2) determine the effect of winter wheat cultivar on the relationship between FHB and DON concentration, and 3) identify the major species of Fusarium causing FHB in Nebraska and characterize its isolates. Differences (P ≤ 0.05) were detected among cultivars in FHB …
Phenotypic Leaf Variation In Avicennia Marina In Tropical Australia: Can Discrete Subpopulations Be Recognised In The Field?, Peter Saenger, Lyndon O. Brooks
Phenotypic Leaf Variation In Avicennia Marina In Tropical Australia: Can Discrete Subpopulations Be Recognised In The Field?, Peter Saenger, Lyndon O. Brooks
Dr Lyndon O Brooks
The geographic patterns of phenotypic variation in leaf morphology traits were studied in the mangrove Avicennia marina (Forsk.) Vierh. in tropical Queensland, Australia, to determine whether discrete subpopulations could be recognised in the field. Significant differences in the various leaf characters occurred among the sites, which were not explained by longitude or latitude, nor by inter-site distances. Hierarchical cluster analysis of the estimated site means showed no coherent geographical groupings of the sites, suggesting that site populations do not follow a differentiation by distance model. Principal component analysis showed that site groupings with consistent leaf morphological characteristics could be identified, …