Digital Commons Network^™

Research Report: Radicchio Cultivar Performance In New Hampshire, Rebecca G. Sideman, Ella Lukacz, Lilly Hartman

Faculty Publications

Radicchio (Cichorium intybus var. latifolium) is grown widely throughout Europe, especially northern Italy. In addition to having interesting color and flavor profiles, they are rich in phenolics and other bioactive compounds. While increasingly popular as a niche vegetable in the region, there is little research-based information to guide growers. The New England growing climate is very different from that of major production regions, and it presents unique challenges. There is tremendous phenotypic variation among and within groups and cultivars. The availability of varieties to commercial growers in the U.S. has expanded greatly, but there remains a need for reliable …

Early Detection, Rapid Response Invasives Management: Documenting Perennial Pepperweed In New Hampshire Using Eddmaps, Final Project Report, December 2022, Malin Clyde

UNH Cooperative Extension

No abstract provided.

Perennial Pepperweek Id Card_2022, Malin Clyde, Charlotte Thompson

UNH Cooperative Extension

No abstract provided.

Research Report: High Tunnel Tomato Fruit Cluster Pruning, Caterina Roman, Rebecca G. Sideman

Faculty Publications

Tomatoes are a high value crop grown worldwide. Indeterminate varieties are commonly grown in high tunnel structures throughout New England for the fresh market. Indeterminate tomato plants often suffer from a phenomenon called ‘June drop’ in which the plant’s first four to five cluster of fruit set perfectly but the subsequent two to three clusters have poor set and plant productivity drops suddenly. While cluster thinning (e.g., reducing the number of fruit allowed to mature per cluster) has been successfully shown to increase fruit size, it has generally not increased marketable yield. We hypothesized that reducing the fruit load by …

Leveraging Natural Resources Toward Resilience: Outreach, Restoration, And Monitoring For A Resilient Nh Coast, Alyson Eberhardt, David M. Burdick, Gregg E. Moore

Faculty Publications

Both sound science and meaningful community engagement are critical to creating resilient coastlines given that important natural resources and high population densities coexist in these areas. Salt marshes and sand dunes provide important benefits to the people who live near them, such as mitigating flood impacts, when the condition of the ecosystem is robust enough to support such functions. To enhance the ability of these systems to perform ecosystem services, the goals of this project were to 1. Empower landowners to sustainably manage their own property to support coastal ecosystems and the benefits they provide, 2. Restore eroded sand dunes …

Sensitivity And Threshold Dynamics Of Pinus Strobus And Quercus Spp. In Response To Experimental And Naturally-Occurring Severe Droughts, Heidi Asbjornsen, Cameron D. Mcintire, Matthew A. Vadeboncoeur, Katie A. Jennings, Adam P. Coble, Z. Carter Berry

Earth Systems Research Center

Increased drought frequency and severity are a pervasive global threat, yet the capacity of mesic temperate forests to maintain resilience in response to drought remains poorly understood. We deployed a throughfall removal experiment to simulate a once in a century drought in New Hampshire, USA, which coupled with the region-wide 2016 drought, intensified moisture stress beyond that experienced in the lifetimes of our study trees. To assess the sensitivity and threshold dynamics of two dominant northeastern tree genera (Quercus and Pinus), we monitored sap flux density (Js), leaf water potential and gas exchange, growth, and intrinsic water use efficiency (iWUE) …

Seaweed Monitoring In The Great Bay Estuary, Nh: 2020 Annual Report, Andrew Payne, David M. Burdick, Gregg Moore, Natalie White, Christopher Peter, Arthur Mathieson

Jackson Estuarine Laboratory

As water temperatures rise due to global warming and nitrogen inputs change, it is important to understand how these changes are impacting vegetative communities that form the basic habitat structure in the Great Bay Estuary, NH. The abundance and taxa of intertidal seaweeds have been monitored at fixed locations throughout the Estuary since 2013. In 2020, percent cover and biomass were collected from five intertidal and four subtidal sampling locations. Data from 2013-2020 show appreciable amounts of nuisance seaweeds (primarily reds), including several introduced species. Cover of green seaweeds decreased significantly over time at the two intertidal sites (Depot Road …

Correcting Tree-Ring Δ13c Time Series For Tree-Size Effects In Eight Temperate Tree Species, Matthew A. Vadeboncoeur, Katie A. Jennings, Andrew P. Ouimette, Heidi Asbjornsen

Earth Systems Research Center

Stable carbon isotope ratios (δ¹³C) in tree rings have been widely used to study changes in intrinsic water-use efficiency (iWUE), sometimes with limited consideration of how C-isotope discrimination is affected by tree height and canopy position. Our goals were to quantify the relationships between tree size or tree microenvironment and wood δ¹³C for eight functionally diverse temperate tree species in northern New England, and to better understand the physical and physiological mechanisms underlying these differences. We collected short increment cores in closed-canopy stands and analyzed δ¹³C in the most recent 5 years of growth. …

Eelgrass Health Survey And Results, Nicholas B. Anderson, Catherine M. Ashcraft, Dante D. Torio, Frederick T. Short

Natural Resources & the Environment

Researchers at the University of New Hampshire designed, tested, and conducted an eelgrass health survey. The primary goal of the survey was to collect health ratings from respondents who had viewed pre-selected images of eelgrass representing a wide range of health conditions. Survey results were used as a calibration and validation to a novel eelgrass health index developed using video-monitoring. Two secondary goals of this this survey were the identification of plant-specific and environmental characteristics important to respondents and the introduction of the new eelgrass health index. This published dataset includes de-identified survey respondent background and demographic data, the survey …

Influence Of Forest-To-Silvopasture Conversion And Drought On Components Of Evapotranspiration, Adam P. Coble, Alexandra R. Contosta, Richard G. Smith, Nathan W. Siegert, Matthew A. Vadeboncoeur, Katie A. Jennings, Anthony J. Stewart, Heidi Asbjornsen

Natural Resources & the Environment

The northeastern U.S. is projected to experience more frequent short-term (1-2 month) droughts interspersed among larger precipitation events. Agroforestry practices such as silvopasture may mitigate these impacts of climate change while maintaining economic benefits of both agricultural and forestry practices. This study evaluated the effects of forest-to-silvopasture (i.e., 50% thinning) conversion on the components of evapotranspiration (transpiration, rainfall interception, and soil evaporation) during the growing season of 2016. The study coincided with a late-summer drought throughout the northeastern U.S., which allowed us to also evaluate the effects of forest-to-silvopasture conversion on drought responses of multiple tree species, including Pinus strobus, …

Hydrological Niche Segregation Defines Forest Structure And Drought Tolerance Strategies In A Seasonal Amazon Forest, Mauro Brum, Matthew A. Vadeboncoeur, Valeriy Ivanov, Heidi Asbjornsen, Scott Saleska, Luciana F. Alves, Deliane Pinha, Jadson D. Dias, Luiz E O C Aragão, Fernanda Barros, Paulo Bittencourt, Luciano Pereira, Rafael S. Oliveira

Earth Systems Research Center

1. The relationship between rooting depth and above‐ground hydraulic traits can potentially define drought resistance strategies that are important in determining species distribution and coexistence in seasonal tropical forests, and understanding this is important for predicting the effects of future climate change in these ecosystems.
2. We assessed the rooting depth of 12 dominant tree species (representing c. 42% of the forest basal area) in a seasonal Amazon forest using the stable isotope ratios (δ¹⁸O and δ²H) of water collected from tree xylem and soils from a range of depths. We took advantage of a major ENSO‐related …

Phosphorus Limitation Of Aboveground Production In Northern Hardwood Forests, Shinjini Goswami, Melany C. Fisk, Matthew A. Vadeboncoeur, Mariann Garrison-Johnston, Ruth D. Yanai, Timothy J. Fahey

Earth Systems Research Center

Forest productivity on glacially derived soils with weatherable phosphorus (P) is expected to be limited by nitrogen (N), according to theories of long-term ecosystem development. However, recent studies and model simulations based on resource optimization theory indicate that productivity can be co-limited by N and P. We conducted a full factorial N × P fertilization experiment in 13 northern hardwood forest stands of three age classes in central New Hampshire, USA, to test the hypothesis that forest productivity is co-limited by N and P. We also asked whether the response of productivity to N and P addition differs among species …

The Development Of Polyamines Throughout Brassica Rapa Over Its Lifecycle, Matthew Ryan Sullivan, Jon Larsen, Subhash C. Minocha

Student Research Projects

Polyamines are naturally produced chemicals in plants involved in growth, development and stress response. The primary objective of my study is to create a profile of changes in the entire life of the plant, in every organ at all stages of development from seed germination to seed formation. We have analyzed polyamines putrescine, spermidine and spermine in all parts of Brassica rapa, a small, rapid growing plant. Parallel to the polyamines, we will also study changes in the activities of the polyamine biosynthetic enzymes and the expression of their genes in different organs at different times. In the next stage …

Salt Marsh Values In A Changing World: Examining Sea Level Rise On Tidal Marshes With A Surface Elevation Table, Sarah Martina Tierney

Student Research Projects

Rising seas are threatening coastal communities and putting added pressures on the natural environment. Sea level rise rates are increasing on a global scale (from 1.7 to 3.2 mm/yr). Salt marshes are not only intertidal habitats acutely influenced by sea levels, but they also provide key ecosystem services such as: buffers against storm surges, habitat for wildlife, carbon dioxide storage, and pollutant filtration. In New England, salt marshes have built at a rate of 1 to 2 mm annually over the past 4,000 years, which has kept pace with sea level rise. However, we do not know if salt marshes …

Overcoming India’S Food Security Challenges: The Role Of Intellectual Property Management And Technology Transfer Capacity Building, Stanley Kowalski, Aarushi Gupta, Ifica Mehra

Law Faculty Scholarship

The growth of the Indian economy after Independence has had little impact on the food security of the country. The paper analyses the development of advanced crop varieties through the use of agricultural technologies (hereinafter "agbiotech") within the technology transfer system, a framework which comprises of the interactions of intellectual property rights law and agricultural research and development in India. Through this, the author argues that agricultural innovation in India is failing due to the absence of connections within the technology transfer system and advocates for the creation of a national program aimed at advancing IP and tech-transfer capacity in …

Phase Ii Of Tidal Marsh Restoration At Steedman Woods Reserve At York, Maine, David M. Burdick, Chris Peter, Gregg Moore

Jackson Estuarine Laboratory

No abstract provided.

Superweeds And Suspect Seeds: Does The Genetically-Engineered Crop Deregulation Process Put American Agriculture At Risk, Margaret Sova Mccabe

Law Faculty Scholarship

The federal government’s regulatory approach to genetically engineered (GE) crops, known as “The Framework”, is now twenty-five years old. Despite two and half decades of a consistent regulatory regime, GE crop and food regulation remains controversial. This article suggests that regulatory science and its tenets of independence, transparency, and public science should guide reforms of The Framework so that it is an efficient and reliable regulatory system. The article has four parts: 1) it provides a brief overview of the history of GE crop regulation; 2)it describes the key attributes of The Framework and related regulatory documents, with particular focus …

Conservation And Loss Of Ribosomal Rna Gene Sites In Diploid And Polyploid Fragaria (Rosaceae), Bo Liu, Thomas M. Davis

Biological Sciences

The genus Fragaria comprises species at ploidy levels ranging from diploid (2n = 2x = 14) to decaploid (2n = 10x = 70). Fluorescence in situ hybridization with 5S and 25S rDNA probes was performed to gather cytogenetic information that illuminates genomic divergence among different taxa at multiple ploidy levels, as well as to explore the evolution of ribosomal RNA genes during polyploidization in Fragaria.

A Strawberry Knox Gene Regulates Leaf, Flower And Meristem Architecture, Mithu Chatterjee, Claudia L. Bermudez-Lozano, Maureen A. Clancy, Thomas M. Davis, Kevin M. Folta

Biological Sciences

The KNOTTED-LIKE HOMEODOMAIN (KNOX) genes play a central role in maintenance of the shoot apical meristem. They also contribute to the morphology of simple and compound leaves. In this report we characterize the FaKNOX1 gene from strawberry (Fragaria spp.) and demonstrate its function in trasgenic plants. The FaKNOX1 cDNA was isolated from a cultivated strawberry (F.×ananassa) flower EST library. The sequence is most similar to Class I KNOX genes, and was mapped to linkage group VI of the diploid strawberry genome. Unlike most KNOX genes studied, steady-state transcript levels were highest in flowers and fruits. Transcripts were also detected in …

An Examination Of Targeted Gene Neighborhoods In Strawberry, Thomas M. Davis, Melanie E. Shields, Qian Zhang, Denise Tombolato-Terzić, Jeffrey L. Bennetzen, Ana C. Pontaroli, Hao Wang, Qin Yao, Phillip Sanmiguel, Kevin M. Folta

Biological Sciences

This report details the structure and content of targeted regions of the strawberry genome. The data indicate that the strawberry genome is gene-dense, with an average of one protein-encoding gene or pseudogene per 5.9 kb. Current overall EST coverage is sparse. The unexpected gene duplications and their differential patterns of EST support suggest possible subfunctionalization or pseudogenization of these sequences. This report provides a high-resolution depiction of targeted gene neighborhoods that will aid whole-genome sequence assembly, provide valuable tools for plant breeders and advance the understanding of strawberry genome evolution.

An Enhanced Method For Sequence Walking And Paralog Mining: Topo® Vector-Ligation Pcr, Benjamin B. Orcheski, Thomas M. Davis

Biological Sciences

Background

Although technological advances allow for the economical acquisition of whole genome sequences, many organisms' genomes remain unsequenced, and fully sequenced genomes may contain gaps. Researchers reliant upon partial genomic or heterologous sequence information require methods for obtaining unknown sequences from loci of interest. Various PCR based techniques are available for sequence walking - i.e., the acquisition of unknown DNA sequence adjacent to known sequence. Many such methods require rigid, elaborate protocols and/or impose narrowly confined options in the choice of restriction enzymes for necessary genomic digests. We describe a new method, TOPO^® Vector-Ligation PCR (or TVL-PCR) that innovatively …

Insertion Sequence Content Reflects Genome Plasticity In Strains Of The Root Nodule Actinobacterium Frankia, Derek M. Bickhart, Johann P. Gogarten, Pascal Lapierre, Louis S. Tisa, Philippe Normand, David R. Benson

Molecular, Cellular & Biomedical Sciences

Background: Genome analysis of three Frankia sp. strains has revealed a high number of transposable elements in two of the strains. Twelve out of the 20 major families of bacterial Insertion Sequence (IS) elements are represented in the 148 annotated transposases of Frankia strain HFPCcI3 (CcI3) comprising 3% of its total coding sequences (CDS). EAN1pec (EAN) has 183 transposase ORFs from 13 IS families comprising 2.2% of its CDS. Strain ACN14a (ACN) differs significantly from the other strains with only 33 transposase ORFs (0.5% of the total CDS) from 9 IS families. Results: Insertion sequences in the Frankia genomes were …

Crop Bioengineering: Enormous Potential For Catalyzing International Development, Peter Gregory, Stanley P. Kowalski

Law Faculty Scholarship

[Excerpt] Bioengineering provides unique and dramatic opportunities for crop improvement. It can be used to develop crop varieties that would otherwise be unavailable and can facilitate much faster and more precise ways of developing improved varieties. It can help to increase yields and reliability and thus reduce food costs for the consumer while helping to control input costs for farmers through reduced applications of herbicides, pesticides, and fertilizer.

The extent to which this will be achieved depends on how effectively the global scientific community – including both the public and private sectors – can cooperate in harnessing the power of …

Impacts Of Marine Docks On Eelgrass In New England: A Spreadsheet-Based Model For Managers And Planners, David M. Burdick, Frederick T. Short, Gregg Moore

Faculty Publications

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Comparative Secretome Analysis Suggests Low Plant Cell Wall Degrading Capacity In Frankia Symbionts, Juliana E. Mastronunzio, Louis S. Tisa, Philippe Normand, David R. Benson

Molecular, Cellular & Biomedical Sciences

Background: Frankia sp. strains, the nitrogen-fixing facultative endosymbionts of actinorhizal plants, have long been proposed to secrete hydrolytic enzymes such as cellulases, pectinases, and proteases that may contribute to plant root penetration and formation of symbiotic root nodules. These or other secreted proteins might logically be involved in the as yet unknown Molecular interactions between Frankia and their host plants. We compared the genome-based secretomes of three Frankia strains representing diverse host specificities. Signal peptide detection algorithms were used to predict the individual secretomes of each strain, and the set of secreted proteins shared among the strains, termed the core …

U.S. Patent Literature Survey Of Agrobacterium-Mediated Transformation Of Sweet Potato (Ipomoea Batatas), Jon R. Cavicchi, Stanley P. Kowalski, J. Jaya Murthy, Rahul R. Vartak

Law Faculty Scholarship

A team of researchers and patent information scientists at Franklin Pierce Law Center were asked to evaluate the patent and literature landscape related to the Agrobacterium-mediated transformation in sweet potato with respect to the U.S. patents and patent applications. This report provides a patent landscape of the Agrobacterium-mediated transformation of sweet potato. The report includes the applicable methods of transformation and has also included certain patents and patent applications which claim a transformed plant by virtue of these methods. In certain cases, the claim structure covers Agrobacterium-mediated transformation technology via system and composition of matter claims and not the more …

Freedom-To-Operate In The Crop Sciences: Procedure, Stanley P. Kowalski

Law Faculty Scholarship

Freedom to operate (FTO) is the ability to proceed with research, development and commercialization of a crop science product, while fully accounting for any potential risks of infringing activity, that is, whether a product can be made, used, sold, offered for sale, or exported, with a minimal risk of infringing the unlicensed intellectual property rights (IPRs) or tangible property rights (TPRs) of another. An FTO analysis begins with the ‘FTO team’ systematically dissecting the crop science product into the components, combination of components, processes and germplasm that went into its research and development. This is followed by generating a series …

Rational Risk/Benefit Analysis Of Genetically Modified Crops, Stanley P. Kowalski

Law Faculty Scholarship

Safety concerns over the use of molecular biotechnology in the improvement of crops has generated substantial, heated and confusing debates, often driven by ideology and hysterics. Modification of crops is not new, and biotechnology (in its broadest sense) has been used for over a century to accelerate the development of new crops for food, feed and fibre, so as to meet the demands of a growing global community. The introduction of crops developed via molecular biotechnology [Genetically Modified Crops (GMCs)] represents the latest step in this inexorable innovative progression of technology. However, misinformed concern has led to a broad embrace …

Fluorescent Flowers: A Long Road To Accomplishment, Lindsay Kendrick

Inquiry Journal 2006

No abstract provided.

Portrait: Anatole Krattiger—Intellectual Property Management In The Global Public Interest, Stanley P. Kowalski

Law Faculty Scholarship

[Excerpt] "What do cows in green Alpine landscapes have in common with IP? Not much unless you ask Dr. Krattiger. As a young farmer in his native Switzerland, and later in the South of France where he cultivated vineyards, he developed a practical approach to solving problems. During these formative years as a farmer, Dr. Krattiger particularly enjoyed tending dairy herds in the green pastures of the Swiss Alps. There he learned and practiced the art of fine cheese making: an age-old and fundamental application of traditional biotechnology. Working in sight of the sublime peaks of the Alps must have …