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Brassica Carinata Growth And Yield Response To Nitrogen And Sulfur Fertilizers And Impacts On Selected Soil Parameters And Ghg Fluxes, Dwarika Bhattarai Jan 2019

Brassica Carinata Growth And Yield Response To Nitrogen And Sulfur Fertilizers And Impacts On Selected Soil Parameters And Ghg Fluxes, Dwarika Bhattarai

Electronic Theses and Dissertations

Carinata (Brassica carinata A. Braun), a non-food oilseed crop and an alternative bio-jet fuel feedstock, has received attention for its potential as a low-input option for production in the semi-arid regions of the Northern Great Plains of USA. The crop has a lower N fertilizer requirement as compared to the other oilseeds, suggesting less negative impact on soils and GHGs emissions. Carinata is a new crop to South Dakota (SD), thus, the best management practices have yet to be developed. In addition, no sufficient research to address the impact of growing carinata on soils and GHG emissions has been …


Increasing Accumulation Of Glyceollins In Soybeans Via Optimization Of The Fungal Incubation Process, Stephanie Wootton Jan 2019

Increasing Accumulation Of Glyceollins In Soybeans Via Optimization Of The Fungal Incubation Process, Stephanie Wootton

Electronic Theses and Dissertations

Continual use of antibiotics in the feed of food animals was viewed a solution to the problem of disease outbreaks in livestock produced in confinement operations. This practice also improved animal performance, likely due to the reduction in sub-clinical infections. Unfortunately, this practice led to a new problem, the development of antibiotic resistant microbes. This increase in antibiotic resistance reduced the direct benefits of antibiotics in animal production. Moreover, as antibiotic resistance spread from animal to human pathogens, this practice created a major public health concern. This led the FDA to enact the Veterinary Feed Directive in 2017 that greatly …


Characterizing Spatiotemporal Patterns Of White Mold In Soybean Across South Dakota Using Remote Sensing, Confiance L. Mfuka Jan 2019

Characterizing Spatiotemporal Patterns Of White Mold In Soybean Across South Dakota Using Remote Sensing, Confiance L. Mfuka

Electronic Theses and Dissertations

Soybean is among the most important crops, cultivated primarily for beans, which are used for food, feed, and biofuel. According to FAO, the United States was the biggest soybeans producer in 2016. The main soybean producing regions in the United States are the Corn Belt and the lower Mississippi Valley. Despite its importance, soybean production is reduced by several diseases, among which Sclerotinia stem rot, also known as white mold, a fungal disease that is caused by the fungus Sclerotinia sclerotiorum is among the top 10 soybean diseases. The disease may attack several plants and considerably reduce yield. According to …


Interactions Of Arbuscular Mychorrhizal Fungi And Bacterial Endophytes On Disease Resistance Of Common Root Pathogens In Wheat, Janice L. Eibensteiner Jan 2019

Interactions Of Arbuscular Mychorrhizal Fungi And Bacterial Endophytes On Disease Resistance Of Common Root Pathogens In Wheat, Janice L. Eibensteiner

Electronic Theses and Dissertations

Arbuscular mycorrhiza, a symbiosis between plants and fungi, help plants to capture nutrients such as phosphorus (P), nitrogen (N) and other micronutrients from the aggre in exchange for up to 20% of the fixed carbon (C) from the plant. In addition, arbuscular mycorrhizal (AM) fungi can improve the resistance against abiotic (drought, salinity), and also biotic (pathogen) stresses. Bacterial endophytes promote plant growth and yield by fixing N2 from the atmosphere, assimilating N and transfer it to the plant. Bacterial endophytes can also solubilize phosphate and stimulate plant defense responses, suppressing pathogens. The host plant provides sucrose and a favorable …


A Review Of The Genus Acrisis Foerster (Hymenoptera: Braconidae) With A New Species From American Licorice And A Survey Of Aphid Parasitoids Of Soybean Fields In Eastern South Dakota, Abigail P. Martens Jan 2019

A Review Of The Genus Acrisis Foerster (Hymenoptera: Braconidae) With A New Species From American Licorice And A Survey Of Aphid Parasitoids Of Soybean Fields In Eastern South Dakota, Abigail P. Martens

Electronic Theses and Dissertations

The diversity of parasitoid wasps (Hymenoptera: Braconidae) in South Dakota is largely unexamined despite these wasps providing innumerable important ecological and biological services and being an essential part of natural and agricultural ecosystems. A new species of Acrisis Foerster was reared and is described from the native prairie legume Glycyrrhiza lepidota (Nuttall) Pursh, a plant studied for its potential for conservation and remediation. A large insect guild of seed predators and their associated parasitoids and hyperparasitoids reported from G. lepidota provides a list of potential hosts associated with the new parasitoid species. The agriculturally significant legume soybean, Glycine max (L.) …


Estimating Soil Organic Carbon In Cultivated Soils Using Soil Test Data, Remote Sensing Imagery From Satellites (Landsat 8 And Plantscope), And Web Soil Survey Data, Muhammed Halil Koparan Jan 2019

Estimating Soil Organic Carbon In Cultivated Soils Using Soil Test Data, Remote Sensing Imagery From Satellites (Landsat 8 And Plantscope), And Web Soil Survey Data, Muhammed Halil Koparan

Electronic Theses and Dissertations

Soil organic carbon (SOC) is an important soil parameter of cultivated soils that needs to be monitored and mapped regularly to enhance soil health and productivity. SOC levels in cultivated areas is difficult to monitor for farmers and is costly to analyze using traditional methods. The objective of this study was to estimate surface SOC distribution in selected soils of Major Land Resource Areas (MLRA) 102A (Rolling Till Plain, Brookings County, SD) and 103 (Central Iowa and Minnesota Till Prairies, Lac qui Parle County, MN) using soil sample data, Web Soil Survey (WSS) data, and satellite imagery (Landsat 8 and …


Identification And Characterization Of Stress Responsive Genes In Soybean And Sunflower, Surendra Neupane Jan 2019

Identification And Characterization Of Stress Responsive Genes In Soybean And Sunflower, Surendra Neupane

Electronic Theses and Dissertations

Stress responsive genes encode proteins involved in plants’ response to abiotic and biotic stresses. Among such stress responsive proteins, proteins encoded by resistance genes (R genes) or nucleotide binding site-leucine-rich repeats (NBS-LRRs) and mitogen-activated protein kinases (MAPKs) are the major groups of proteins regulating biotic and abiotic stresses, respectively. Previous studies in Nepal’s lab at SDSU identified and characterized coiled coil (CC)-NBS-LRRs (CNLs), resistance to powdery mildew8 (RPW8)-NBS-LRRs (RNLs), NBS-LRR (NLs), and MAPK proteins in soybean. This study focuses on R and MAPK genes in the recently sequenced genome of sunflower as well as the toll-interleukin-1 receptor-like nucleotide-binding site leucine-rich …


Development And Characterization Of Wheat-Thinopyrum Junceiforme Chromosome Addition Lines, Dilkaran Singh Jan 2019

Development And Characterization Of Wheat-Thinopyrum Junceiforme Chromosome Addition Lines, Dilkaran Singh

Electronic Theses and Dissertations

Production of wheat is challenged by dynamic biotic and abiotic stresses. Genetic improvement via alien gene transfer is an effective approach to tackle such challenges. Alien gene transfer played an important role in the history of wheat crop improvement. Sea wheatgrass (SWG; Thinopyrum junceiforme, 2n = 28, genomes J1J1J2J2) is a wild relative of wheat. In our previous work, we have developed a complete amphiploid between cultivated emmer and SWG and shown that SWG is resistant to wheat streak mosaic virus, Fusarium head blight and wheat stem sawflies (due to the solid stem) and tolerant to waterlogging, salinity, heat, and …


Efficacy Of Synthetic And Biopesticides On Bacteria Leaf Streak Management And Influence Of Cultivar And Environment On Epiphytic Bacteria Diversity On Wheat Seeds, Marilen Nampijja Jan 2019

Efficacy Of Synthetic And Biopesticides On Bacteria Leaf Streak Management And Influence Of Cultivar And Environment On Epiphytic Bacteria Diversity On Wheat Seeds, Marilen Nampijja

Electronic Theses and Dissertations

Wheat (Triticum aestivum) is the most important staple food for over two billion people, or 36% of the world population. The United States ranks 4th in the world for wheat production. However, wheat production is faced by both biological and non-biological constraints. Among the biological constraints, diseases play a big role in limiting wheat production with estimated yield losses ranging from 20% to 40%. Bacterial leaf streak (BLS) of wheat caused by Xanthomonas translucens pv undulosa (Xtpvu) is one of the major bacterial diseases affecting the wheat production in most wheat growing regions in the USA. In recent years, it …


Development Of Computational Techniques For Identification Of Regulatory Dna Motif, Cankun Wang Jan 2019

Development Of Computational Techniques For Identification Of Regulatory Dna Motif, Cankun Wang

Electronic Theses and Dissertations

Identifying precise transcription factor binding sites (TFBS) or regulatory DNA motif (motif) plays a fundamental role in researching transcriptional regulatory mechanism in cells and helping construct regulatory networks for biological investigation. Chromatin immunoprecipitation combined with sequencing (ChIP-seq) and lambda exonuclease digestion followed by high-throughput sequencing (ChIP-exo) enables researchers to identify TFBS on a genome-scale with improved resolution. Several algorithms have been developed to perform motif identification, employing widely different methods and often giving divergent results. In addition, these existing methods still suffer from prediction accuracy. Thesis focuses on the development of improved regulatory DNA motif identification techniques. We designed an …


A Seed Dormancy Gene-Mediated Biotechnology To Mitigate Transgene Flow Into Weedy Rice, Luai Nahar Muhammad Jan 2019

A Seed Dormancy Gene-Mediated Biotechnology To Mitigate Transgene Flow Into Weedy Rice, Luai Nahar Muhammad

Electronic Theses and Dissertations

Transgene flows into wild/weedy relatives may cause ecological and economic problems. Seed dormancy is an adaptive trait that distributes germination over time and promotes persistence of weeds in agroecosystems. Silencing natural genes controlling seed dormancy (SD) could promote germination and reduce weed adaptability. The goal of this project was to develop a transgenic mitigation (TM) technology by linking to a primary transgene with an SD gene-silencing structure, as a tandem construct for transformation, to reduce the risk of gene flow into weed populations. In this research, the Bar (Bialaphos) gene for resistance to the glufosinate herbicide was used as a …


Stoichiometric Homeostasis In Two Native And Two Invasive South Dakotan Grasses, Joshua Thonas Harvey Jan 2019

Stoichiometric Homeostasis In Two Native And Two Invasive South Dakotan Grasses, Joshua Thonas Harvey

Electronic Theses and Dissertations

Increased nutrient availability has been widely linked to the success of invasive plants, however a general mechanism explaining these observations is lacking. Stoichiometric homeostasis (H), which is the regulation of internal nutrient concentrations, has been used to explain changes in plant community diversity under alterations in nutrient availability. One hypothesis holds that plants with high regulation (larger H) decrease in abundance in nutrient enriched conditions but are stable in nutrient deficient and drought conditions, likely due to extensive root systems. Additionally, plants with low regulation (lower H) increase in abundance under nutrient enriched conditions but are sensitive to drought …


Validation Of Candidate Loci For Maize Regrowability And Selection Of Near Isogenic Lines For The Loci, Tajbir Raihan Jan 2019

Validation Of Candidate Loci For Maize Regrowability And Selection Of Near Isogenic Lines For The Loci, Tajbir Raihan

Electronic Theses and Dissertations

Developing perennial grain crops is an effective way for sustainable agriculture and it can be a tool for the betterment of the overall ecosystem. To understand how perenniality works in perennial species, we made a cross between Zea diploperennis and maize (Z. mays) inbred line B73. F1 hybrids produced from this cross were selfed to get the F2 population and later the F2 population underwent Genotyping-By- Sequencing (GBS) analysis. Based on the candidate loci identified on chromosome 2 and chromosome 7 from GBS, SNP-specific markers were developed from the candidate locus interval and used to genotype the F2 …


Sunflower Pollinators In South Dakota: An Evaluation Of Species Composition, Abundance And Influence On Yield, Mackenzie Mattern Jan 2019

Sunflower Pollinators In South Dakota: An Evaluation Of Species Composition, Abundance And Influence On Yield, Mackenzie Mattern

Electronic Theses and Dissertations

In 2017 and 2018, South Dakota was the largest producer of sunflowers, Helianthus annuus L. (Asterales: Asteraceae) in the US, grossing approximately $178 million per year. In South Dakota, both confection and oilseed sunflower cultivars are grown. Despite breeding efforts to increase sunflower's ability to self-pollinate there is evidence that pollinator visitation results in increased sunflower yields. In addition, there is evidence that sunflower pollen is beneficial for pathogen resistance in various bee species, suggesting the importance of a mutualistic relationship between pollinators and sunflowers. In some areas, observed increases in sunflower yield is attributed to the presence of Apis …


Long-Term Impacts Of Manure And Inorganic Fertilization On Soil Physical, Chemical And Biological Properties, Asmita Gautam Jan 2019

Long-Term Impacts Of Manure And Inorganic Fertilization On Soil Physical, Chemical And Biological Properties, Asmita Gautam

Electronic Theses and Dissertations

The intensive use of mineral fertilizers to achieve high crop yield has led to soil degradation and poor soil health. Thus, manure application as an alternative to mineral fertilizers can be an effective fertilization strategy to improve soil health and biodiversity. This study aims to assess the impacts of long-term manure and mineral fertilizers on soil physical, chemical and biological properties. The experimental site was initiated in 2003 near Beresford, South Dakota on Egan soil under a randomized complete block design with four replications and six treatments. The study treatments included: three manure rates [low manure (LM), manure application based …


Nodule Zone-Specific Gene Expression In Soybean, Sadikshya Aryal Jan 2019

Nodule Zone-Specific Gene Expression In Soybean, Sadikshya Aryal

Electronic Theses and Dissertations

Nitrogen is one of the most limiting nutrients for plant growth and yield. Leguminous plants such as soybean (Glycine max) have developed the ability to form a symbiotic association with nitrogen fixing rhizobia. This symbiotic association results in the formation of unique structures called nodules that originate from root cortex via de novo cell differentiation. During soybean nodule development, two major nodule zones, the Nodule primordium (Npr) in the center and the nodule parenchyma (Npa) in the periphery, are clearly distinguishable. Npr gives rise to infection zone (IZ), and the Npa holds vascular bundles. However, it is not clear what …


Some Studies On Leaf Spot Of Oats And Triticale, Mohammed Abdullah Lashram Jan 2019

Some Studies On Leaf Spot Of Oats And Triticale, Mohammed Abdullah Lashram

Electronic Theses and Dissertations

Oat is an important cereal crop, and it is considered to be among the highest used in cereal crops. It is considered among the healthiest grains due to the rich source of the soluble fiber, β-glucan that helps in lowering the cholesterol. Oat production and demand have increased considerably in the past few years due to its health benefits. South Dakota is ranked in the top three largest oat-producing states in the USA. Oat is relatively less susceptible to pest and diseases except for leaf diseases such as crown rust, Drechslera avenae leaf spot, and Stagonospora avenae leaf blotch. Leaf …


Agronomic And Quality Response Of Hard Red Spring Wheat Genotypes To Management Systems In South Dakota, Jonathan Kleinjan Jan 2019

Agronomic And Quality Response Of Hard Red Spring Wheat Genotypes To Management Systems In South Dakota, Jonathan Kleinjan

Electronic Theses and Dissertations

Intensive cereals management techniques such as multiple fungicide applications and in-season split N applications have been used to successfully improve wheat yields in Europe and in some winter wheat production areas of the USA. However, research on the effects of these management practices and their interaction with genotypes is limited in the hard red spring wheat (HRSW) production areas of the USA. The objectives of this study were to: (i) compare management treatments and (ii) quantify any interaction effects between management and genotype on the agronomic characteristics and relevant flour and dough properties of locally-adapted HRSW genotypes. A randomized complete …


Cropping Sequence Affects Subsequent Soybean Yield, Soil Microbiome And Soil Health, Izzet Bulbul Jan 2019

Cropping Sequence Affects Subsequent Soybean Yield, Soil Microbiome And Soil Health, Izzet Bulbul

Electronic Theses and Dissertations

Rotation is an important cultural practice that farmers use to manage crop growth and diseases. Diversified crop rotations directly affect plant residue inputs that may enrich specific groups of microbes that form beneficial associations with the following crop. At two field sites, yield differences were observed in soybeans planted after the following four long-term (14 year) crop sequences: continuous corn (CC), corn (Zea mays)/corn/soybean (Glycine max) (CCS), corn/soybean/corn (CSC), soybean/corn/soybean (SCS). Soybean yields were in decreasing order, CC>CCS>CSC>SCS, and the yield differences could not be explained by soil chemical and physical properties previously …


Disease Resistance In Wheat And Its Relatives, Ethan James Andersen Jan 2019

Disease Resistance In Wheat And Its Relatives, Ethan James Andersen

Electronic Theses and Dissertations

Plants have evolved a complex defense system against pests and pathogens utilizing many types of receptors, signaling factors, and defense compounds to detect pathogen presence and respond effectively. Since many pathogens have evolved immunesuppressing effectors used to reduce plant resistance, plants have evolved a family of receptors that detect pathogenic effectors as a result of an evolutionary arms race. These receptors contain Nucleotide-Binding Site and Leucine-Rich Repeat domains and are called NBS-LRR or NLR proteins. Many grasses possess huge genomes with hundreds of NLR-encoding genes, often found in clusters at the extra-pericentromeric regions of chromosomes, where unequal crossing over causes …


Quality Evaluations Of Hard Red Spring Wheat Grown In South Dakota, Lance Merrick Jan 2019

Quality Evaluations Of Hard Red Spring Wheat Grown In South Dakota, Lance Merrick

Electronic Theses and Dissertations

Hard Red Spring Wheat (HRSW; Triticum aestivum L.) is characterized by generally high protein content and excellent baking and milling performance. The ability for millers, bakers, and other end-users of wheat to properly and cheaply produce food products with uniformity is dependent on the quality consistency of wheat produced by breeders and farmers. Determining how production years and locations influenced enduse quality traits will assist breeders, agronomists, and growers, in understanding which hard red spring wheat (HRSW; Triticum aestivum L.) varieties are least susceptible to quality fluctuations, and therefore, most desirable to end-users. In order to determine which varieties were …


Diversifying Cornfields By Interseeding Cover Crops: Practical Implications And The Response Of Invertebrate Communities, Michael Bredeson Jan 2019

Diversifying Cornfields By Interseeding Cover Crops: Practical Implications And The Response Of Invertebrate Communities, Michael Bredeson

Electronic Theses and Dissertations

The lack of suitable habitat conditions for beneficial organisms in simplified agroecosystems leads to unstable invertebrate communities and overreliance on chemical control of herbivores. It is possible to manage pest populations without agrichemicals by manipulating farmland so that herbivores are impaired by plant-driven bottom-up and enemy-driven top-down antagonisms. Interseeding cover crops between established crop rows is a method used by farmers to improve habitat suitability for natural enemies and hinder host-finding, feeding and movement by herbivores. Here I address three important research gaps related to interseeding cover crops. A calcium carbonate seed coating, used to improve seed-broadcasting efficiency, was tested …


Isolation And Identification Of Potential Bioinoculants Based On Phosphate Solubilizing And Plant Growth Promoting Benefits, Rachel Raths Jan 2019

Isolation And Identification Of Potential Bioinoculants Based On Phosphate Solubilizing And Plant Growth Promoting Benefits, Rachel Raths

Electronic Theses and Dissertations

Conservative models have shown that as populations rise, food production needs to double by 2050. Population increase and the green revolution have caused fertilizer inputs to increase since the 1960’s, increasing environmental issues and production costs. These intensive practices have led to degraded arable land and there has been an increase in urbanization meaning we need to make best use of the farming land that is available and ensure it is sustainable for future food production. Due to this, there has been a higher demand for research on more environmentally and economically friendly approaches to food production. Plant growth promoting …


Species Of Fusarium Causing Root Rot Of Soybean In South Dakota: Characterization, Pathogenicity, And Interaction With Heterodera Glycines, Paul Nyawanda Okello Jan 2019

Species Of Fusarium Causing Root Rot Of Soybean In South Dakota: Characterization, Pathogenicity, And Interaction With Heterodera Glycines, Paul Nyawanda Okello

Electronic Theses and Dissertations

In South Dakota, Fusarium-associated diseases (e.g. seedling diseases, root rot and sudden death syndrome) are emerging threats to soybean production. Several species of Fusarium have been reported to cause these diseases in the soybean producing regions of the United States and in the world. However, little information is available on the species of Fusarium causing soybean root rot in South Dakota and their pathogenicity. Therefore, the objectives of this study were to (1) characterize the species of Fusarium causing soybean root rot in South Dakota; (2) evaluate the cross-pathogenicity of species of Fusarium causing root rot of soybean and corn; …