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Forage Quality Of Alfalfa Protected By Resistance To Bacterial Leaf Spot, Andrew W. Lenssen, E. L. Sorensen, G. L. Posler, D. L. Stuteville Jan 1992

Forage Quality Of Alfalfa Protected By Resistance To Bacterial Leaf Spot, Andrew W. Lenssen, E. L. Sorensen, G. L. Posler, D. L. Stuteville

Andrew W. Lenssen

Bacterial leaf spot (BLS), caused by Xanthomonas campestris pv. arfalfae Dye (Xca ), can prevent stand establishment and decrease the yield and persistence of alfalfa (Medicago sativa L.) in the central USA. Previous studies have shown that BLS inoculation may affect concentrations of monophenolic compounds and thus alter the forage quality of alfalfa. In a controlled environment, comparisons were made of forage quality, including phenolic monomers, of alfalfa cv. Kanza (susceptible) and KS76 germplasm (derived from Kanza, resistant) with and without inoculation with Xca. The cultivarx inoculation interactions were non-significant (P> 0.05 ) for all parameters determined for leaves and …


Basic Alfalfa Germplasms Differ In Nutritive Content Of Forage, Andrew W. Lenssen, E. L. Sorensen, G. L. Posler, L. H. Harbers Mar 1991

Basic Alfalfa Germplasms Differ In Nutritive Content Of Forage, Andrew W. Lenssen, E. L. Sorensen, G. L. Posler, L. H. Harbers

Andrew W. Lenssen

Little information is available regarding comparative forage quality of alfalfa (Medicago sativa L.) leaves and stems of the nine germplasms from which most North American cultivars have been developed. In a greenhouse trial, forage quality of leaves and stems of the nine germplasms was compared when grown in a common environment and harvested at the same phenological stage. Germplasm sources (and representative cultivar) tested were: Indian (Sirsa #9), African (African), Peruvian (Hairy Peruvian), Flemish (DuPuits), Turkistan (Lahontan), Chilean (Kansas Common), M. varia Martin (Grimm), Ladak (Ladak), M. falcata L. (Anik). Leaves of germplasm sources differed for concentrations of neutral detergent …


Depression Of Forage Quality Of Alfalfa Leaves And Stems By Acyrthosiphon Kondoi (Homoptera: Aphididae), Andrew W. Lenssen, Edgar L. Sorensen, Gerry L. Posler, Sue L. Blodgett Jan 1991

Depression Of Forage Quality Of Alfalfa Leaves And Stems By Acyrthosiphon Kondoi (Homoptera: Aphididae), Andrew W. Lenssen, Edgar L. Sorensen, Gerry L. Posler, Sue L. Blodgett

Andrew W. Lenssen

ACYTthosiphon kondoi Shinji is a major pest of alfalfa (Medicago sativa L.). Susceptible seedling plants are killed by A. kondoi, and established plants suffer yield loss by stunting. Information is meager on the influence of A. kondoi infestation or host plant resistance on forage quality of alfalfa leaves and stems. In a controlled environment, the forage quality (includingphenolic monomers)of alfalfagermplasmsKSI53P4and KS153BA3P4 (susceptible and resistant to A. kondoi, respectively) with and without A. kondoi infestation was compared. Leaves infested with A. kondoi had lower concentrations of crude protein and true in vitro digestible dry matter (TIVDDM) than uninfested leaves of controls, …


Resistance To Anthracnose Protects Forage Quality Of Alfalfa, Andrew W. Lenssen, G. L. Posler, E. L. Sorenson, D. L. Stuteville Jan 1991

Resistance To Anthracnose Protects Forage Quality Of Alfalfa, Andrew W. Lenssen, G. L. Posler, E. L. Sorenson, D. L. Stuteville

Andrew W. Lenssen

Anthracnose, caused by Colletotrichum trifolii Bain, decreases forage yield and stand persistence of alfalfa (Medicago sativa L.) throughout much of the USA. Little is known about the effects of anthracnose or host-plant resistance on forage quality, including phenolic monomer concentrations. In a controlled environment, we compared forage quality, including phenolic monomers, of alfalfa cultivars Saranac (susceptible) and Saranac AR (resistant) with and without anthracnose (Race 1). In the absence of inoculation, leaves of the two cultivars did not differ significantly for any parameter measured. Leaves had more total phenolics than did stems. Forage quality of inoculated leaves of Saranac often …


Stratification Not Required For Tree-Of-Heaven Seed Germination, William R. Graves Jan 1990

Stratification Not Required For Tree-Of-Heaven Seed Germination, William R. Graves

William R Graves

Tree-of-heaven (Ailanthus altissima (Mill.) Swingle) seed germination and shoot dry weight were determined after stratifying samaras at 5 and 25 °C for up to 28 days. Seeds of samaras stratified at 5 °C for 0, 4, and 12 days showed 70, 77, and 96% germination, respectively, 7 days after sowing. Fourteen days after sowing, 90% of nonstratified seeds had germinated, but shoot weight of nonstratified seedlings was 16% less than that of seedlings from fruit stratified at 5 °C for 12 days. Germination 7 days after sowing was greater after stratification at 5 °C than at 25 °C, but the …


Forage Quality Of Genetically Diverse Alfalfa Germplasms At Four Phenological Growth Stages, Andrew W. Lenssen, E. L. Sorensen, G. L. Posler Jan 1990

Forage Quality Of Genetically Diverse Alfalfa Germplasms At Four Phenological Growth Stages, Andrew W. Lenssen, E. L. Sorensen, G. L. Posler

Andrew W. Lenssen

Forage quality of various alfalfa (Medicago sativa L.) cultivars has been determined under different environmental conditions, and numerous trials with alfalfa have documented an inverse relationship between forage quality and maturity. Little information is available, however, regarding the comparative forage quality of the germplasm sources from which most USA cultivars were developed. We compared forage quality of these germplasm sources at four phenological stages under disease- and insect-free conditions in a greenhouse. Germplasm sources (cultivars) tested were: Indian (Sirsa # 9), African (African), Peruvian (Hairy Peruvian), Flemish (DuPuits), Turkistan (Lahontan), Chilean (Kansas Common), M. varia (Grimm), and Ladak (Ladak). Four …


Sheep Preference For Perennial Glandular-Haired And Eglandular Medicago Populations, Andrew W. Lenssen, E. L. Sorensen, G. L. Posler, L. H. Harbers Jan 1989

Sheep Preference For Perennial Glandular-Haired And Eglandular Medicago Populations, Andrew W. Lenssen, E. L. Sorensen, G. L. Posler, L. H. Harbers

Andrew W. Lenssen

Erect glandular-haired Medicago species and hybrid populations have been developed that possess a high degree of resistance to the alfalfa weevil [Hypera postica (Gyllenhal)] and potato leafhopper [Empoasca fabae (Harris)]. Trials were conducted at the North Agronomy Farm, Manhattan, KS, in June and August 1986 to determine if erect glandular hairs on diverse perennial Medicago populations affect forage preference of sheep (Ovis aries). Glandular-haired (+) and eglandular (−) populations were selected from each of the following: diploids M. prostrata Jacq. and M. glandulosa David (KS94) and tetraploids M. glutinosa Bieb. (KS108), M. sativa L. ✕ M. prostrata (KS159), and M. …


In Vitro Dry Matter Disappearance, Crude Protein Concentration, And Leaf Percentage Of Erect Glandular-Haired Medicago Populations, Andrew W. Lenssen, E. L. Sorensen, G. L. Posler, L. H. Harbers Jan 1988

In Vitro Dry Matter Disappearance, Crude Protein Concentration, And Leaf Percentage Of Erect Glandular-Haired Medicago Populations, Andrew W. Lenssen, E. L. Sorensen, G. L. Posler, L. H. Harbers

Andrew W. Lenssen

Available resistance in alfalfa (Medicago sativa L.) cultivars is currently inadequate to control the alfalfa weevil, Hypera postica (Gyllenha), or potato Ieafhopper, ErnpoascaJabae (Harris), the two most injurious arthropod pests of alfalfa in North America. Resistance to both insects has been documented in other Medicago species having erect, glandular hairs. These hairs have been transferred to alfalfa. A field trial in 1985 was to determine the effects of erect, glandular hairs and their exudates on forage quality of several perennial Medicagos. Glandular and eglandular plant populations were selected from each of the diploids, M. prostrata Jacq. and M. glandulosa David., …


Molecular-Marker-Facilitated Investigations Of Quantitative-Trait Loci In Maize. I. Numbers, Genomic Distribution And Types Of Gene Action, Jonathan F. Wendel, M. D. Edwards, Charles W. Stuber May 1987

Molecular-Marker-Facilitated Investigations Of Quantitative-Trait Loci In Maize. I. Numbers, Genomic Distribution And Types Of Gene Action, Jonathan F. Wendel, M. D. Edwards, Charles W. Stuber

Jonathan F. Wendel

Individual genetic factors which underlie variation in quantitative traits of maize were investigated in each of two F 2 populations by examining the mean trait expressions of genotypic classes at each of 17-20 segregating marker loci. It was demonstrated that the trait expression of marker locus classes could be interpreted in terms of genetic behavior at linked quantitative trait loci (QTLs). For each of 82 traits evaluated, QTLs were detected and located to genomic sites. The numbers of detected factors varied according to trait, with the average trait significantly influenced by almost two-thirds of the marked genomic sites. Most of …


Forage Quality Of Perennial Glandular-Haired And Eglandular Medicago Populations, Andrew W. Lenssen Jan 1987

Forage Quality Of Perennial Glandular-Haired And Eglandular Medicago Populations, Andrew W. Lenssen

Andrew W. Lenssen

No abstract provided.


Additional Mapping Of Isozyme Loci: Localization Of Acp4, Dia2, Adkl, Tpi3, And Sadl, Jonathan F. Wendel, Major M. Goodman, Charles W. Stuber Jan 1986

Additional Mapping Of Isozyme Loci: Localization Of Acp4, Dia2, Adkl, Tpi3, And Sadl, Jonathan F. Wendel, Major M. Goodman, Charles W. Stuber

Jonathan F. Wendel

We recently listed the isozyme loci being studied by starch gel electrophoresis in our laboratory and summarized the available mapping data in a series of reports (Wendel et al., MGCNL 59:87-90). Subsequent work has resulted in further clarification of the chromosomal locations of markers on chromosomes 1 (Acp4, Dia2), 6 (Adkl), 8 (Tpi3), and 10 (Sadl). Previously unreported information on these loci and their chromosomal locations follows.


Tpi4 Is Located Near The Centromere On The Long Arm Of Chromosome 3, Jonathan F. Wendel, J. B. Beckett Jan 1986

Tpi4 Is Located Near The Centromere On The Long Arm Of Chromosome 3, Jonathan F. Wendel, J. B. Beckett

Jonathan F. Wendel

Tpi4, one of the three genes encoding cytosolic triose phosphate isomerase isozymes, was earlier shown to be on 3L between Pgd2 (phosphogluconate dehydrogenase) and the centromere (Wendel et al., MNL 59:88). In an effort to better localize Tpi4, a series of crosses was made between Tpi4 testers and stocks carrying three B-A translocations believed to be near the centromere on 3L.


Localization Of Two New Isozyme Loci, Hexl And Tpi4, To Chromosome 3, Jonathan F. Wendel, Charles W. Stuber, Major M. Goodman Jan 1985

Localization Of Two New Isozyme Loci, Hexl And Tpi4, To Chromosome 3, Jonathan F. Wendel, Charles W. Stuber, Major M. Goodman

Jonathan F. Wendel

Recent work with starch gel electrophoresis of coleoptile extracts has indicated that Hexl, the structural locus for the most anodal set of hexokinase isozymes, and Tpi4, which encodes the slowest migrating set of triose phosphate isomerase bands, are located on chromosome 3.


Mapping Data For 34 Isozyme Loci Currently Being Studied, Jonathan F. Wendel, Charles W. Stuber, Major M. Goodman Jan 1985

Mapping Data For 34 Isozyme Loci Currently Being Studied, Jonathan F. Wendel, Charles W. Stuber, Major M. Goodman

Jonathan F. Wendel

With the recent localization of several new loci, a composite listing of these loci and chromosomal locations has been compiled and is shown in Table 1. Although locations of some of the loci are tentative, many are very precisely located from studies involving 1600 to more than 1900 F2 plants.


Linkage Relationships Between A New Locus, Hex2, And Previously Assigned Loci On Chromosome 6, Jonathan F. Wendel, Charles W. Stuber, Major M. Goodman Jan 1985

Linkage Relationships Between A New Locus, Hex2, And Previously Assigned Loci On Chromosome 6, Jonathan F. Wendel, Charles W. Stuber, Major M. Goodman

Jonathan F. Wendel

Starch gel electrophoresis of maize coleoptile extracts in our laboratory has shown two zones of staining for isozymes of hexokinase. Genetic analyses 9 indicate that the slower migrating set of bands is encoded by the structural gene Hex2 and that the enzyme is monomeric. Recent linkage analyses and work with B-A translocation stocks (generously supplied by Jack Beckett) have allowed for the placement ofHex2 on the long arm of chromosome 6. Its location relative to other markers on 6L was determined from the analysis of two very large F2 populations generated for other reasons.


Twelve New Isozyme Loci In Maize: Progress Report On Chromosomal Locations, The Subunit Composition, And Subcellular Localization Of Their Products, Jonathan F. Wendel, Charles W. Stuber, Major M. Goodman Jan 1985

Twelve New Isozyme Loci In Maize: Progress Report On Chromosomal Locations, The Subunit Composition, And Subcellular Localization Of Their Products, Jonathan F. Wendel, Charles W. Stuber, Major M. Goodman

Jonathan F. Wendel

To improve the degree to which the com genome is biochemically marked, we have expanded the number of enzyme systems analyzed by starch gel electrophoresis. Seven new enzyme systems encoded by a minimum of 16 loci have been added to the 23 loci used for previous work in our laboratory. A wide range of domestic and exotic maize has been screened for variation at these new loci and genetic analysis of variant electromorphs is well under way. Crosses with B-A translocation stocks (kindly provided by Jack Beckett), previously mapped allozyme loci, and in a few cases morphological markers, are being …


Genetic Divergence Within The Genus Liriodendron (Magnoliaceae), Jonathan F. Wendel, Clifford R. Parks, Norton G. Miller, Karen M. Mcdougal Jan 1983

Genetic Divergence Within The Genus Liriodendron (Magnoliaceae), Jonathan F. Wendel, Clifford R. Parks, Norton G. Miller, Karen M. Mcdougal

Jonathan F. Wendel

The genus Liriodendron L. consists of a southeast Asian-eastern North American disjunct species pair, but the genus had a much wider distribution in the Northern Hemisphere during the late Cretaceous and the Tertiary. Although generally similar in morphology, the two extant species are measurably different. In 1973 and 1977 they were hybridized, and interspecific heterosis was observed in the progeny. After seven years, the interspecific hybrids synthesized in 1973 had a significantly greater biomass than intraspecific hybrids of L. tulipifera. The growth rate of interspecific and intraspecific F2 seedlings was approximately equal. A preliminary survey of flavonoid extracts in L. …


Modeling The Kinetics Of Heat Inactivation Of Trypsin Inhibitors During Steam-Infusion Cooking Of Soymilk, Lawrence A. Johnson, W. J. Hoover, C. W. Deyoe, L. E. Erickson, W. H. Johnson, J. R. Schwenke Mar 1980

Modeling The Kinetics Of Heat Inactivation Of Trypsin Inhibitors During Steam-Infusion Cooking Of Soymilk, Lawrence A. Johnson, W. J. Hoover, C. W. Deyoe, L. E. Erickson, W. H. Johnson, J. R. Schwenke

Lawrence A. Johnson

Theeffect of heat on trypsin inhibitor (TI) activity during steam-infusion cooking of soymilk was studied. At 154°C only 40 s process time reduced TI to the same level as conventional cooking at 99°C for 60 min. Between 99 and 154°C the kinetics of TI inactivation followed behavior exemplified by the summation of two first-order reactions. Spline fitting functions effectively modeled the data with r2 ranging from 0.984 to 0.999. The heat-labile reaction was attributed to Kunitz inhibitor and the heat-stable reaction, to Bowman-Birk inhibitor. The former accounted for approximately 85 percent of the iriginal TI activity. Arrhenius equation kinetic constants …


Removal Of Raw Peanut Flavor And Odor In Peanut Flour Processed By Direct Solvent Extraction1, Lawrence A. Johnson, J. T. Farnsworth, R. J. Garland, E. W. Lusas Jan 1979

Removal Of Raw Peanut Flavor And Odor In Peanut Flour Processed By Direct Solvent Extraction1, Lawrence A. Johnson, J. T. Farnsworth, R. J. Garland, E. W. Lusas

Lawrence A. Johnson

Peanut slices can be directly extracted with hexane yielding more soluble protein and better color than pre-press solvent extraction; however, flavor and odor are characterized as raw and “green beany”. The utilization of secondary extraction with hexane: ethanol azeotrope, hexane: methanol azeotrope and absolute ethanol subsequent to hexane extraction significantly improved flavor and odor characteristics of peanut flour. Hexane: propanol azeotrope did not significantly improve sensory evaluations. Hexane: ethanol azeotrope did not reduce soluble protein, yielding an NSI value of 95%. Hexane: methanol azeotrope and absolute ethanol slightly reduced NSI to 88% and 92% respectively. Color of peanut flour was …