Digital Commons Network^™

Improving Aedes Mosquito Surveillance And La Crosse Virus Screening In Eastern Tennessee, Cassandra Urquhart

Masters Theses

La Crosse virus (LACV), transmitted by infected Aedes triseriatus, Ae. albopictus, and Ae. japonicus mosquitoes is the leading cause of pediatric arboviral encephalitis. Severe cases of LAC encephalitis occur in individuals 16-years-old or younger and may cause permanent neurological damage or fatality. No vaccines exist making mosquito control and disease prevention crucial to public health. Effective screening and surveillance practices are key components to these goals. While a number of standard mosquito surveillance methods exist, continuous testing and improved understanding of vector biology to determine the best ways to implement these methods is important. Additionally, the current standard …

Farm-To-Preschool In East Tennessee: Evaluation Of A Small-Scale Farmer’S Market At A Childcare Center On Household Fruit And Vegetable Availability And Preschoolers’ Consumption Of Fruits And Vegetables, Jade Morgan Parry

Masters Theses

Background: Most preschool-aged children do not consume an adequate amount of fruits and vegetables (F&V). Accessibility to F&V and parental influence are important factors in determining fruit and vegetable consumption in preschool-aged children. The incorporation of farmer’s markets at childcare centers as part of a farm-to-preschool program provides an opportunity to increase access to F&V and engage parents in supporting positive changes in their children’s dietary behaviors. Gaps in the farm-to-preschool literature include a lack of well-designed intervention trials that include a control group and rigorous evaluation tools.

Methods: This was a pilot study with a quasi-experimental, pre-test, post-test …

A Feasibility Study Of Just-In-Time Asthma Self-Management Intervention, Phillip Charles Scruggs

Masters Theses

Quality asthma care requires not only an initial diagnosis, but long term follow up care and education on long term control. Preventable symptoms continue to reduce the quality of life for chronic asthma sufferers and place an unnecessary burden on emergency services and the health care system at large. A mobile health (mHealth) solution is proposed to effectively improve outcomes for patients while ultimately reducing the overall burden of mismanagement on the healthcare system as a whole. The Just-in-Time Asthma Self- Management and Intervention (JASMIN) system provides a way for a patient or user to track, self-manage, and interact with …

The Effects Of A Visually Appealing And Interactive Snack Activity On Fruit And Vegetable Intake Of Preschool-Aged Children, Kristen Leigh Clay

Masters Theses

Background: Previous research has shown the potential of increasing vegetable intake by incorporating the senses into interventions. However, little to no research has specifically looked at the impact that the use of the senses can have on intake of vegetables among preschool-aged children.

Purpose: The aims of this study were to examine if mean intake of vegetable snacks and/or willingness to try the vegetable snacks would be significantly higher among preschool-age children who participated in a visually appealing and interactive snack activity compared to children who received control vegetable snacks.

Methods: Two preschools were selected to participate in the study. …

The Effect Of Television Watching And Portion Size On Intake During A Meal, Rachel Nicole Rosenthal

Masters Theses

Background: Several investigations have examined the influence of television and portion size on intake. Results have found that watching television while eating or being provided larger portions increases intake. However, no investigation has examined the combined effect of these variables on food intake, when these factors are combined they may enhance consumption.

Methods: To test the influence of television and portion size on intake during a meal in healthy weight adults, a 4X2X2 mixed factorial design was used, with a between-subject factor of order of conditions and within-subject factors of television (TV vs. NO TV) and portion size (SMALL vs. …

Examining The Relationship Between Vitamin A Intake And Weight Management, Jennifer Paige Eastep

Masters Theses

Introduction: Treatments of retinoic acid, a metabolite of vitamin A (VA) are shown to induce hyperlipidemia in humans and animals, which may influence adiposity. To understand the role of VA in weight management, two studies, one in animals examining effects of VA combined with high-fat diet (HFD) on body mass (BM) gain and the second in humans comparing dietary VA between participants who were overweight/obese and who had and had not lost 10% of body weight by consuming a hypocaloric, low-fat diet, were conducted.

Methods: Male Sprague Dawley rats were fed a HFD containing sufficient VA (HF-VAS) or a HFD …

Identification Of Feline Tritrichomonas Foetus Surface Epitopes: Putative Targets For Development Of A Novel Diagnostic Test For Feline Trichomonosis, Emily Nissa Gould

Masters Theses

BACKGROUND: Tritrichomonas foetus (T. foetus) is a flagellated protozoa that infects the distal ileum and proximal colon of domestic cats and also induces reproductive failure in cattle. Although feline trichomonosis is recognized to have a global prevalence of up to 30%, it still poses a diagnostic and therapeutic challenge to veterinarians; thus, there is a need for both improved diagnostics and therapeutics. Despite differing organ tropism between genotypes, evidence exists for conserved virulence factors between feline and bovine T. foetus. Two epitopes (1.15 and 1.17) of the bovine T. foetus glycosylated surface antigen 1.15-1.17 have been found …

Perceived Benefits Of Therapeutic Recreation Camps For Young Adults With Disabilities, Macie Dean Parsons

Masters Theses

The purpose of this study is to determine the perceived benefits that a Therapeutic Recreation camp offers young adults with disabilities. A total of 12 campers were interviewed with 6 being male and 6 being female. Emerging themes included: (a) Social, (b) New Experiences, (c) Character Building, and (d) Get Away. Findings identified the benefits that young adults receive from attending a Therapeutic Recreation camp program. Attending a Therapeutic Recreation camp program allows for individuals with disabilities to be around friends, to meet new friends, to feel a part of a family, to do things they have never done before, …

Improving Ventricular Catheter Design Through Computational Fluid Dynamics, Sofy Hefets Weisenberg

Masters Theses

Cerebrospinal fluid (CSF) shunts are fully implantable medical devices that are used to treat patients suffering from conditions characterized by elevated intracranial pressure, such as hydrocephalus. In cases of shunt failure or malfunction, patients are often required to endure one or more revision surgeries to replace all or part of the shunt. One of the primary causes of CSF shunt failure is obstruction of the ventricular catheter, a component of the shunt system implanted directly into the brain's ventricular system. This work aims to improve the design of ventricular catheters in order to reduce the incidence of catheter obstruction and …

Effects Of Varus Knee Alignment And Using Toe-Cages On Frontal Plane Knee Biomechanics In Stationary Cycling, Guangping Shen

Masters Theses

Effects of varus knee alignment on the internal knee abduction moment (KAM) in walking has been widely studied. KAM has been shown to be closely associated with the development of medial knee osteoarthritis (OA). Despite the importance of the knee alignment, no studies have explored its effects on knee frontal plane biomechanics during stationary cycling. The purpose of this study was to examine the effects of varus knee alignment and using a toe-cage on the knee frontal plane biomechanics during stationary cycling. Eleven participants in each of the varus and neutral groups participated in the study. The participants performed in …

Icloudecg: A Mobile Cardiac Telemedicine System, David S. Clifford

Masters Theses

With rising healthcare costs and a substantially growing number of patients 65 or over, the benefits of telemedicine and patient self-monitoring systems are becoming increasingly evident. Patients, physicians, hospitals, and even insurance providers benefit from vigilant, cost-effective patient monitoring systems. This thesis describes the development of a portable, smart-phone connected system for continuous cardiac monitoring. The system is capable of continuously monitoring the conditions of the heart, automated detection of cardiac arrhythmias, and real-time notifying patients and physicians of the detected abnormalities. The system consists of four main subsystems: 1) a Bluetooth capable chest-strap ECG, 2) an Android-enabled mobile device, …

Associations Of Cooking Self-Efficacy And Frequency Of Icook-4h Youth Participants With Dietary Quality And Bmi At Baseline, Amber Donaldson Ford

Masters Theses

Background: With increased obesity has come increased ready-made and fast food consumption and decreased homemade food consumption. Previous studies have shown associations between cooking self-efficacy (SE) and cooking frequency (CF) with dietary quality and weight status. Cooking interventions have shown positive associations with dietary outcomes, such as increased fruit and vegetable intake and decreased fast food consumption. There is still much unknown about SE and CF, especially among youth.

Objective: The objective of this project was to determine baseline SE and CF and the associations with dietary quality and body mass index (BMI) of youth enrolled in iCook 4-H.

Methods: …

Do Increases In Fruit Intake Lead To Substitutive Eating Patterns And Decreases In Overall Energy Intake In Normal Weight Adults?, Shireen Amanda Sobhani

Masters Theses

Background: It is suggested that increasing intake of fruits and vegetables may lead to decreased energy intake via substitution of higher energy-dense foods, such as snack foods (SF; i.e. candy, chips, cookies). This study investigated the impact of increasing fruit (F; grapes) intake, via increased portion size, on SF (potato chips) intake during a meal and whether increases in F intake affected total meal energy intake, via reducing SF intake (substitutive eating), in normal-weight adults. A secondary objective investigated whether the relative reinforcing value (RRV) of SF was a moderator of the substitutive relationship between F and SF.

Methods: Using …

Enzyme Catalyzed Alginate Nanogels For Drug Delivery, Danna Nichole Sharp

Masters Theses

Developing nanoscale carriers for the delivery of therapeutics is an important topic of investigation in current biomedical research. As opposed to traditional drug delivery systems, nanoscale systems offer enhanced tissue and cell permeation in addition to reducing drug elimination from the body. Biological based therapeutics such as DNA and proteins are now widely employed in medical applications and research has focused on using nanoscale drug delivery systems to administer these more effectively. Current synthesis methods of nanoscale biotherapeutic carriers face significant challenges. Among these are creating carriers with: sizes between 10-200 nm, low polydispersity, and non-cytotoxic materials. In this thesis, …

Nutrition Marketing On The Front-Of-Package: 2007-2014, Ana Elizabeth Hoffmann

Masters Theses

Objective: The objectives of this research were to describe the proportion of packaged foods with front-­‐of package nutrition marketing or marketing to children that were high in saturated fat, sodium, and/or sugar content, and to delineate changes in the proportions of these foods from 2007 to 2014.

Methods: Front-­‐of-­‐pack nutrition marketing, child marketing, and nutrient criteria from the Nutrition Facts Label on all packaged food items in a large Midwestern supermarket were recorded in 2007 (n = 5,500) and again in 2014 (n = 6,324). Products were coded as high/not high in saturated fat, sodium, and/or sugar according to agreed …

Biodegradable Nano-Hybrid Polymer Composite Networks For Regulating Cellular Behavior, Charles Henley Sprague

Masters Theses

Photo-crosslinkable polymeric biomaterials have emerged in the field of biomedical research to promote tissue regeneration. For example, scaffolds that can be crosslinked and hardened in situ have been known to make suitable implant alternatives. Since injectable and photo-crosslinkable biomaterials offer the advantage of being minimally invasive, they have emerged to compete with autografts, a current highly invasive method to repair diseased tissue. A series of novel photo-crosslinkable, injectable, and biodegradable nano-hybrid polymers consisting of poly(ε-caprolactone fumarate) (PCLF) and polyhedral oligomeric silsesquioxane (POSS) has been synthesized in our laboratory via polycondensation. To engineer the material properties of the nano-hybrid networks, varied …

A Computational, Topological Approach To Icu Mortality Rate Prediction With Data Relationship Realization, Adam Michael Aaron

Masters Theses

The objective of this work is to predict the mortality of intensive care unit patients based on their physiological data and understand the relationships between physiological data. Such a model may be used to prioritize care when resources are limited or identify patients that will need significant care in the immediate future. This effort will take a novel approach applying computational topological analysis to classify patients. The algorithm predicting the patient outcomes is trained using an evolutionary algorithm. The dataset used is from the 2012 PhysioNet Computing in Cardiology Challenge. A set containing 4000 records with outcomes was used to …

Effect Of A Core Conditioning Program On Lumbar Paraspinal Area, Asymmetry And Pain Score In Military Working Dogs With Lumbosacral Pain, Andrea Leigh Henderson

Masters Theses

Introduction: Lumbosacral pain and stenosis are common causes of retirement from duty for Military Working Dogs (MWDs). Working dogs that receive surgical management for this condition often have a poor prognosis for return to duty after recovery. Humans with chronic low back pain demonstrate paraspinal muscle asymmetry, pain and dysfunction that often improve in response to an exercise program. This study investigated whether dogs with mild lumbosacral pain have decreased lumbar paraspinal muscle area, symmetry, and density, as well as increased pain and dysfunction compared to control dogs. Additionally, response of pain and dysfunction to an exercise program was assessed. …

Differentiation Of Equine Mesenchymal Stromal Cells Into Cells Of Neural Lineage And Their Application Into A Novel Model For Acute Peripheral Nerve Injury In The Horse, Claudia Cruz

Masters Theses

Studies have shown that mesenchymal stromal cells (MSCs) are able to differentiate into extra-mesodermal lineages, including neurons. Positive outcomes were obtained after transplantation of neurally-induced MSCs in rats, rabbits and guinea pigs after nerve injury, but the effect of these cells is unknown in horses. Our objective was to test the ability of equine mesenchymal stromal cells to differentiate into cells of neuronal lineage, and to assess differences, if any, in morphology and protein expression. Additionally, we wanted to investigate if horse age and cell passage number contributed to the ability to achieve neural differentiation.

The first part of this …

Attention, Hyperactivity, And Cognitive Performance In Adults With Attention-Deficit/Hyperactivity Disorder (Adhd), Brianna Elizabeth Pollock

Masters Theses

The extent to which Attention-Deficit/Hyperactivity Disorder (ADHD) symptoms (i.e., inattention, hyperactivity/impulsivity) are associated with ADHD-related cognitive impairments has yet to be understood. This study sought to examine between-group differences in activity level, performance on a sustained attention task and objectively measured attention, while controlling for anxiety and depression severity in a sample of adults with and without ADHD (N = 26). High precision actigraphs and behavioral codes of visual attention to task were used to examine the extent to which activity level and visual attention to task are related to performance on a sustained attention task (i.e., Continuous Performance …

Social Support In An Internet-Based Weight Loss Intervention Among College Students, Yijia Zhang

Masters Theses

Objective: To assess college students’ perceptions regarding online social support in an 8-week Internet-based weight loss intervention.

Participants: Participants were those randomized to the intervention group of an online weight loss intervention targeting overweight and obese college students.

Methods: Members of the intervention group had access to a private discussion forum and weekly live chat sessions, allowing for anonymous interactions. Following the 8-week intervention, intervention group members were invited to complete an online focus group, discussing these tools. The transcripts were directly copied from the live chat conversation history. Using thematic analysis, perceptions regarding online social support were identified, coded …

Effects Of Cycling Workload And Cadence On Frontal Plane Knee Load, Ying Fang

Masters Theses

The effect of workload and cadence on sagittal plane knee biomechanics in cycling has been widely studied, however few studies have focused on the frontal plane. Frontal plane knee biomechanics, especially knee abduction moment, is closely related to the severity and progression of knee osteoarthritis. The purpose of this study was to investigate the effects of different workloads and cadences on knee frontal plane kinematics and kinetics. Eighteen subjects participated in this study. A motion analysis system was used to collect five cycles of kinematics during two minutes of cycling in eight testing conditions, which included five workload conditions of …

Accuracy Of Sensewear Armband Mini-Fly For Estimating Energy Expenditure In Normal Weight, Overweight, And Obese Individuals, Bethany Maryalice Forseth

Masters Theses

Obesity has become one of the greatest public health issues in America. BodyMedia promotes their SenseWear Armband physical activity monitor as a way to help with weight management. Purpose: to assess the accuracy of the SenseWear Armband Mini-Fly (SWA-MF) in adults of different BMI categories during rest, recovery, and two walking speeds. Methods: Forty-six participants were assigned to one of three BMI classifications: normal weight (n =15; 18.5-24.9 kg^.m^-2 [kilograms per meter squared]), overweight (n=17; 25-29.9 kg^.m^-2), or obese (n= 14; ≥30 kg^.m^-2). Height and weight were measured. Participants …

The Energy Expenditure And Feasibility Of Two Prenatal Yoga Programs, Doree Lynn Gardner

Masters Theses

Purpose: To determine the energy expenditure and feasibility of two different DVD-­‐ based prenatal yoga programs (static yoga practice and vinyasa practice). Methods: Participants were 25 women in their second trimester (13-­‐28 weeks) who werescreened for pregnancy-­‐related risk factors. The two DVDs used were “Prenatal Yoga with Shiva Rea” (DVD A) which is a static yoga practice, and “Jennifer Wolfes Prenatal Vinyasa Yoga” (DVD B) which is a flow yoga practice. Both DVDs were approximately 50-­‐55 minutes, and included three phases (warm up, main, cool down). Each subject completed both prenatal DVD routines approximately two weeks apart. The …

College Environment Behavioral And Perceptions Survey (Cebps): Using In-Depth Interviews As A Validation Method For Survey Development, Mackenzie Rae Ruppert

Masters Theses

Background: Although there are some validation procedures based in qualitative approaches (e.g. asking what someone thinks about the structure of a survey question during face, content validation, or cognitive interviews), there are no approaches to validate a subject’s responses by a “gold standard assessment”. The purpose of this study was to use a mix-methodology approach to validate subjects’ perceptions, which used indepth interviews as a criterion-based validation method.

Methods: 30 participants (15 male and 15 female) completed the CEBPS survey and an indepth interview with questions mirroring those in the survey. A priori coding was used to develop …

Joint Reaction Force And Contributions Of Surrounding Muscles To Knee Joint Load During Stair Ascent In Total Knee Replacement Patients And Healthy Individuals, Robert Jacob Rasnick

Masters Theses

Total knee replacement (TKR) is commonly used to correct end stage knee osteoarthritis (OA) of the knee joint. Unfortunately, difficulty with stair climbing has been seen to exist, prolonging the challenges of TKR patents. Complete understanding of loading at the knee is of great interest in order to aid patient populations, implant manufacturers, rehabilitation, and future research. The outcome of a TKR is intended to reestablish normal motion and loading of the knee. Musculoskeletal modeling provides a means to accurately approximate joint loading and the corresponding muscle contributions during a movement.

The purpose of the present study was to examine …

Biological Variation Of Thromboelastography Variables In Ten Healthy Female Horses, Jennifer Lee Scruggs

Masters Theses

Biological variability (BV) has important applications in laboratory medicine. It can be a source of variation in measured analyte values and provide guidance on reference interval use. BV has three components: between-individual variation (CVg), caused by differences in mean values of a particular analyte among members of a group, within-individual variation (CVi), caused by fluctuations around an individual’s inherent homeostatic set point, and analytical variation (CVa). Thromboelastography (TEG), a type of viscoelastic coagulation analysis, is becoming increasingly common in veterinary referral centers. Despite increased popularity, the optimal method of results interpretation is not clear. While population-based reference intervals (PRI) are …

Biomechanical Difference Between Chronic Ankle Instability Individuals And Healthy Individuals During Landing On Flat, Inverted And Combined Surfaces, Xuan Liu

Masters Theses

Lateral ankle sprains most frequently occurs during sports. Individuals who experienced a first time ankle sprain had a high reoccurrence rate and residual symptoms and functional instability leading to chronic ankle instability (CAI). The purpose of this study was to investigate kinematic and kinetic differences between CAI individuals and healthy subjects in single-leg drop landing on a flat surface, an inverted surface and a combined surface of inversion and plantarflexion. A total of 17 subjects (6 subjects with chronic ankle instability, 11 healthy subjects) performed five trails in each of four dynamic movement conditions of drop landing from a height …

Knee Biomechanics Of Older Adults During Common Recreational And Daily Activities, Julie Lynn Pfeiffer

Masters Theses

Among older adults, socialization is a key motivating factor for participation in physical activity. In order to develop evidence-based physical activity recommendations for older adults with knee pathologies a better understanding of knee biomechanics during social recreational activities is needed. The purpose of this study was to determine knee joint kinematics and kinetics in healthy older adults during golf and bowling. Knee biomechanics during these recreational activities were also compared to those experienced during activities of daily living. Motion analysis data were collected as subjects performed walking, stair ascent, stair descent, and either golf or bowling. Knee angles, peak knee …

Mechanism Of Lpa-Induced Lipid Uptake In Macrophages, Kan Xu

Masters Theses

Cardiovascular disease, currently the leading cause of mortality throughout the developed countries, is mainly caused by atherosclerosis, which is recognized as a chronic inflammatory disease. Atherosclerotic plaques are characterized by accumulations of lipid in arterial walls together with infiltration of macrophages. These macrophages differentiate from monocytes which transform into foam cells through phagocytizing various forms of lipid, are believed to be the main component of early atherosclerotic lesions.

Lysophosphatidic acid (LPA), a potent bioactive lipid, regulates a broad range of cellular functions in various cell types. In our findings, LPA-induced macrophages may enhance the lipid uptake effect in both J774A.1-mouse …