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Increased Matrix Synthesis Following Adenoviral Transfer Of A Transforming Growth Factor Beta1 Gene Into Articular Chondrocytes, Franklin D. Shuler Md, Phd, Helga I. Georgescu, Christopher Niyibizi, Rebecca K. Studer, Zhibao Mi, Brian Johnstone, Paul D. Robbins, Christopher H. Evans

Orthopaedics

Monolayer cultures of lapine articular chondrocytes were transduced with first-generation adenoviral vectors carrying lacZ or transforming growth factor β₁ genes under the transcriptional control of the human cytomegalovirus early promoter. High concentrations of transforming growth factor β₁ were produced by chondrocytes following transfer of the transforming growth factor β₁ gene but not the lacZ gene. Transduced chondrocytes responded to the elevated endogenous production of transforming growth factor β₁ by increasing their synthesis of proteoglycan, collagen, and noncollagenous proteins in a dose-dependent fashion. The increases in collagen synthesis were not accompanied by alterations in the collagen phenotype; …

Visual-Vestibular Habituation And Balance Training For Motion Sickness, Rose Marie Rine, Michael C. Schubert, Thomas J. Balkany

Physical Therapy Faculty Research

Background and Purpose. This case report describes physical therapy for motion sickness in a 34-year-old woman. The purpose of the report is twofold: (1) to provide an overview of the literature regarding motion sickness syndrome, causal factors, and rationale for treatment and (2) to describe the evaluation and treatment of a patient with motion sickness.

Case Description and Outcomes. The patient initially had moderate to severe visually induced motion sickness, which affected her functional abilities and prevented her from working. Following 10 weeks of a primarily home-based program of visualvestibular habituation and balance training, her symptoms were alleviated and she …

Intracellular Reactive Oxygen Species Mediate The Linkage Of Na+/K+-Atpase To Hypertrophy And Its Marker Genes In Cardiac Myocytes, Joseph I. Shapiro, Amir Askari, Zijian Xie, Peter Kometiani, Jie Li, Jiang Liu

Pharmaceutical Science and Research

We showed before that in cardiac myocytes partial inhibition of Na⁺/K⁺-ATPase by nontoxic concentrations of ouabain causes hypertrophy and transcriptional regulations of growth-related marker genes through multiple Ca²⁺-dependent signal pathways many of which involve Ras and p42/44 mitogen-activated protein kinases. The aim of this work was to explore the roles of intracellular reactive oxygen species (ROS) in these ouabain-initiated pathways. Ouabain caused a rapid generation of ROS within the myocytes that was prevented by preexposure of cells to N-acetylcysteine (NAC) or vitamin E. These antioxidants also blocked or attenuated the following actions of …

Transfer Of Lacz Marker Gene To The Meniscus, Hideyuki Goto Md, Franklin D. Shuler Md, Phd, Chanin Lamsam Md, Hans D. Moller Md, Christopher Niyibizi Phd, Freddie H. Fu Md, Paul D. Robbins Phd, Christopher H. Evans Phd

Orthopaedics

Background: Lesions in the avascular two-thirds of the meniscus do not heal well and are of concern clinically. Various growth factors promote the synthesis of matrix by meniscal cells and thus have the potential to augment healing. However, their clinical application is severely hindered by problems with delivery. An attractive approach to overcoming such problems is to transfer genes that encode the growth factors in question to the site of the injury. As a prelude to this, we evaluated methods for delivering genes to the meniscus.

Methods: Gene transfer was evaluated in vitro and in vivo with a lacZ marker …

Contrasting Views Of Physicians And Nurses About An Inpatient Computer-Based Provider Order-Entry System, Todd W. Gress, Michael Weiner, David R. Thiemann, Mollie Jenckes, Stephanie L. Reel, Steven F. Mandell, Eric B. Bass

Internal Medicine

Objective: Many hospitals are investing in computer-based provider order-entry (POE) systems, and providers’ evaluations have proved important for the success of the systems. The authors assessed how physicians and nurses viewed the effects of one modified commercial POE system on time spent patients, resource utilization, errors with orders, and overall quality of care.

Design: Survey.

Measurements: Opinions of 271 POE users on medicine wards of an urban teaching hospital: 96 medical house officers, 49 attending physicians, 19 clinical fellows with heavy inpatient loads, and 107 nurses.

Results: Responses were received from 85 percent of the sample. Most physicians and nurses …

The Cardiovascular And Metabolic Adaptations To Iso-Caloric Moderate Intensity And High Intensity Exercise, Summerson Desmond

Theses, Dissertations and Capstones

Cardiovascular disease is the leading cause of death in the industrialized nations, and accounts for 1 million deaths in the United States each year (McArdle, Katch & Katch, 1991). One of the major risk factors for cardiovascular disease is physical inactivity, which is a behavioral modified risk factor. Physical inactivity plagues 59% of the people in the United States (ACSM Resource Manual, 1998). Physical inactivity often leads to obesity, diabetes, hypertension and hypercholemia, and places sedentary individuals at more risk for cardiovascular disease (CVD), or coronary heart disease (CHD). The question which remains unclear is the quantity and quality of …

A Cross-Validation Of The Marshall University Step Test On College Males And Females Age 18-24, Mary Robin Hampton

Theses, Dissertations and Capstones

Positive health entails more than the absence of disease; it is a state of physical, mental, and social well-being (Hartung, Krock, Crandall, Bisson, & Myhre, 1993; Payne & Hahn, 1995). Physical fitness, as described by Francis (1987), is the potential for making adequate functional adjustments to increased metabolic demands. Cardiorespiratory fitness (aerobic fitness) is believed to be the single best indicator of physical fitness (Astrand & Rodale, 1985). The standard index of cardiorespiratory fitness is maximum oxygen uptake (VO^^), or the maximum rate at which oxygen can be consumed. VO2max is dependent upon the cardiovascular system’s ability to deliver blood …

Interaction Of Laminin Beta2 With Eps8 And Desmin In Muscle Cells, Luke Xueliang Cui

Theses, Dissertations and Capstones

Laminin β2 is localized to the synaptic basal lamina (BL) in muscle and glomerular BL in kidney. To find interacting proteins, a mouse kidney cDNA library was screened with domain I of rat laminin β2 using the yeast two-hybrid system. Fifteen positive clones were found. The DNA sequence of three each of these clones corresponded to the phosphoprotein Eps8 (epidermal growth factor receptor pathway substrate clone 8, which is tyrosine phosphorylated after EGF stimulation) and the intermediate filament (IF) protein keratin. To determine if this interaction occurred in muscle cells, C2 cells transfected with the rat …

Depression In Women With Heart Disease, Kimberly M. Ashcraft

Theses, Dissertations and Capstones

The purpose of this study was to examine the relationship between anxiety and anger with depression in women with heart disease. A sample of 50 women participating in cardiac rehabilitation programs in Charleston, WV and Huntington, WV was used to evaluate the inter-relationship of suppressed, expressed, state and trait anger, state and trait anxiety and depression. This non-experimental, descriptive designed study was evaluated by using multiple linear regression and regression coefficients analyzed for the anger and anxiety scores and their interaction. Depression was measured by the Beck Depression Inventory II (BDI-II). Anxiety and anger were measured by Spielberger’s State-Trait Anxiety …