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On-Line Wear Debris Detection In Lubricating Oil For Condition Based Health Monitoring Of Rotary Machinery, Li Du, Jiang Zhe Apr 2015

On-Line Wear Debris Detection In Lubricating Oil For Condition Based Health Monitoring Of Rotary Machinery, Li Du, Jiang Zhe

Dr. Jiang Zhe

Condition based monitoring has become essential in maintaining and extending the health of high-speed rotating and reciprocating machinery. One effective approach to detect signs of potential failure of a rotating or reciprocating machine is to examine the wear debris in its lubricating oil. Recent patents and research articles on on-line wear debris detection in lubricating oil are reviewed in this paper, including electrical impedance detection, acoustic detection, optical detection, pressure detection, online x-ray spectrography, resonant frequency detection, and electrostatic charge detection. Advantages and limitations of each detection method are discussed.


A High Throughput Inductive Pulse Sensor For Online Oil Debris Monitoring, Li Du, Jiang Zhe Apr 2015

A High Throughput Inductive Pulse Sensor For Online Oil Debris Monitoring, Li Du, Jiang Zhe

Dr. Jiang Zhe

A high throughput inductive pulse sensor based on inductive Coulter counting principle for detecting metallic wear debris in lubrication oil is presented. The device detects the passage of metallic debris by monitoring the inductance change of a two-layer planar coil with a meso-scale fluidic pipe crossing its center, which is designed to attain high throughput without sacrificing the sensitivity. The testing results using iron and copper particles ranging in size from 50 to 150 μm have demonstrated that the device is capable of detecting and distinguishing ferrous and non-ferrous metallic debris in lubrication oil with a high throughput.


Capacitive Coulter Counting: Detection Of Metal Wear Particles In Lubricant Using A Microfluidic Device, Srinidhi Murali, Xingao Xia, Ashish Jagtiani, Joan Carletta, Jiang Zhe Apr 2015

Capacitive Coulter Counting: Detection Of Metal Wear Particles In Lubricant Using A Microfluidic Device, Srinidhi Murali, Xingao Xia, Ashish Jagtiani, Joan Carletta, Jiang Zhe

Dr. Jiang Zhe

A microfluidic device based on the capacitance Coulter counting principle to detect metal debris particles in lubricant oil is presented. The device scans each individual metal debris particle as they pass through a microfluidic channel by monitoring the capacitance change. We first proved the feasibility of using the capacitance Coulter counting principle for detecting metal particles in a fluidic channel. Next, we tested the microfluidic device with aluminum abrasive particles ranging from 10 to 25 µm; the testing results show the microfluidic device is capable of detecting metal wear particles in low-conductive lubricant oil. The design concept demonstrated here can …


A Capillary Microgripper Based On Electrowetting, Abhay Vasudev, Jiang Zhe Apr 2015

A Capillary Microgripper Based On Electrowetting, Abhay Vasudev, Jiang Zhe

Dr. Jiang Zhe

An electrowetting based capillary microgripper, which can pick up and release micro-objects, is presented. Electrowetting was utilized to dynamically change the contact angle between the liquid bridge and the gripper surface to control the capillary lifting forces. A coplanar interdigitated goldelectrode pair was employed to simplify the microfabrication. The lifting force generated by the microgripper was experimentally characterized. The pickup and release routine was demonstrated for various micro glass beads whose weight ranges from 77 to 136 μN. The design, microfabrication, and testing of the microgripper are presented.


A Microfluidic Multichannel Resistive Pulse Sensor Using Frequency Division Multiplexing For High Throughput Counting Of Micro Particles, Ashish Jagtiani, Joan Carletta, Jiang Zhe Apr 2015

A Microfluidic Multichannel Resistive Pulse Sensor Using Frequency Division Multiplexing For High Throughput Counting Of Micro Particles, Ashish Jagtiani, Joan Carletta, Jiang Zhe

Dr. Jiang Zhe

In this work we demonstrate an on-chip multiplexed multichannel resistive pulse sensor (Coulter counter) for high throughput counting of microscale particles. The design, fabrication and testing of this device are presented. The high throughput counting is a result of using multiple parallel microfluidic channels to analyze the sample. Detection is achieved by using frequency division multiplexing; each microchannel is modulated with its own known and unique frequency, a combined measurement is made across a single pair of electrodes, and the measured signal is demodulated to determine the signal across each individual channel. Testing results using 30 µm polystyrene particles demonstrate …


Postsynthetic Surface Functionalization, Postsynthetic Surface Functionalization, Encapsulation, And Releasing Studies Of A Novel Polymer Nanocapsule, Duangruthal Sridaeng, Jacob Weingart, Nuanphun Chantarasiri, Jiang Zhe, J. Hu Apr 2015

Postsynthetic Surface Functionalization, Postsynthetic Surface Functionalization, Encapsulation, And Releasing Studies Of A Novel Polymer Nanocapsule, Duangruthal Sridaeng, Jacob Weingart, Nuanphun Chantarasiri, Jiang Zhe, J. Hu

Dr. Jiang Zhe

Postsynthetic surface functionalizations of the amphiphilic poly(allyamine)-g-poly(t-butylacrylate) nanocapsule of 50–100 nm diameters are reported. The hydrophobic poly(t-butylacrylate) surface of the polymer nanocapsules were successfully functionalized with poly(acrylate) and poly(ethylene glycol). These new nanocapsules were investigated for their water dispersibility, encapsulation and controlled releasing properties with fluorescein, an anionic fluorescence probe, and 5-fluorouacil, an anticancer drug as the model compounds. The postsynthetic surface funcitionalization approach of the cross-linked polymer nanocapsules shows promise for improving the biocompatibility and aqueous dispersibility of the nanocapules without disrupting the encapsulation and releasing properties of the polymer nanocapsules.


Novel Quantitative Macro Biomolecule Analysis Based On A Micro Coulter Counter, Y Han, H. Wu, F. Liu, Gang Cheng, Jiang Zhe Apr 2015

Novel Quantitative Macro Biomolecule Analysis Based On A Micro Coulter Counter, Y Han, H. Wu, F. Liu, Gang Cheng, Jiang Zhe

Dr. Jiang Zhe

We demonstrate quantitative biomolecule analysis using a micro coulter counter. Specific binding between antibody functionalized microparticles and target biomolecule cause large aggregates of microparticles. The micro coulter counter was employed to measure aggregation ratio, ratio of the aggregate counts to the total particle counts. Goat anti-rabbit IgG, used as a model biomarker, were tested in this paper. The experiment results showed that the aggregation ratio increases with the increasing biomolecule concentration, and the detectable concentration range from 16 to 160 ng/ml was achieved.


An Impedimetric Approach For Accurate Particle Sizing Using A Micro Coulter Counter, Ashish Jagtiani, Joan Carletta, Jiang Zhe Apr 2015

An Impedimetric Approach For Accurate Particle Sizing Using A Micro Coulter Counter, Ashish Jagtiani, Joan Carletta, Jiang Zhe

Dr. Jiang Zhe

In this paper, we present the design, impedimetric characterization and testing of a microfabricated Coulter counter for particle size measurement that uses a pair of thin film coplanar Au/Ti electrodes. An electrical equivalent circuit model of the designed device is analyzed. Accurate measurement of particle size was achieved by operating the device at a frequency for which the overall impedance is dominated by the channel resistance. A combination of design features, including the use of a pair of sensing electrodes with a surface area of 100 µm by 435 µm, a spacing of 1785 µm between the two sensing electrodes …


Wavelet Transform-Based Methods For Denoising Of Coulter Counter, Ashish Jagtiani, Rupesh Sawant, Joan Carletta, Jiang Zhe Apr 2015

Wavelet Transform-Based Methods For Denoising Of Coulter Counter, Ashish Jagtiani, Rupesh Sawant, Joan Carletta, Jiang Zhe

Dr. Jiang Zhe

A process based on discrete wavelet transforms is developed for denoising and baseline correction of measured signals from Coulter counters. Given signals from a particular Coulter counting experiment, which detect passage of particles through a fluid-filled microchannel, the process uses a cross-validation procedure to pick appropriate parameters for signal denoising; these parameters include the choice of the particular wavelet, the number of levels of decomposition, the threshold value and the threshold strategy. The process is demonstrated on simulated and experimental single channel data obtained from a particular multi-channel Coulter counter processing. For these example experimental signals from 20 µm polymethacrylate …


Real-Time Monitoring Of Wear Debris In Lubrication Oil Using A Microfluidic Inductive Coulter Counting Device, Li Du, Jiang Zhe, Joan Carletta, Robert Veillette, Fred Choy Apr 2015

Real-Time Monitoring Of Wear Debris In Lubrication Oil Using A Microfluidic Inductive Coulter Counting Device, Li Du, Jiang Zhe, Joan Carletta, Robert Veillette, Fred Choy

Dr. Jiang Zhe

A microfluidic device based on an inductive Coulter counting principle to detect metal wear particles in lubrication oil is presented. The device detects the passage of ferrous and nonferrous particles by monitoring the inductance change of an embedded coil. The device was tested using iron and copper particles ranging in size from 50 to 125 μm. The testing results have demonstrated that the device is capable of detecting and distinguishing ferrous and nonferrous metal particles in lubrication oil; such particles can be indicative of potential machine faults in rotating and reciprocating machinery.


Soft Microgripping Using Ionic Liquid Droplet For High Temperature And Vacuum Applications, Abdullah Amin, Ashish Jagtiani, Abhay Vasudev, Jun Hu, Jiang Zhe Apr 2015

Soft Microgripping Using Ionic Liquid Droplet For High Temperature And Vacuum Applications, Abdullah Amin, Ashish Jagtiani, Abhay Vasudev, Jun Hu, Jiang Zhe

Dr. Jiang Zhe

Aqueous droplet-based micro-gripper has been used for micro-assembly. However they cannot be used for high temperature and vacuum applications. Ionic liquids, organic salts that have a lower melting point temperature, appear suitable for droplet-based micro-gripping application in high temperature and vacuum environments because of their nonvolatility and thermal stability. In this paper, we demonstrated the use of ionic liquid as the operating liquid for micro-gripping applications in high temperatures (up to 110 °C) and vacuum (up to 24 inch Hg) environments. Electrowetting was utilized to dynamically change the contact angle of a 1-butyl-3-methylimidazolium hexafluorophosphate (BmimPF6) liquid bridge to control the …


Static And Dynamic Responsive Behavior Of Polyelectrolyte Brushes Under External Electrical Field, Hui Ouyang, Zhenhai Xia, Jiang Zhe Apr 2015

Static And Dynamic Responsive Behavior Of Polyelectrolyte Brushes Under External Electrical Field, Hui Ouyang, Zhenhai Xia, Jiang Zhe

Dr. Jiang Zhe

The static and dynamic behaviors of partially charged and end-grafted polyelectrolyte brushes in response to electric fields were investigated by means of molecular dynamics simulation. The results show that the polymer brushes can be partially or fully stretched by applying an external electric field. Moreover, the brushes can switch reversibly from collapsed to stretched states, fully responding to the AC electric stimuli, and the gating response frequency can reach a few hundred MHz. The effects of the grafting density, the charge fraction of the brushes and the strength of the electric field on the average height of the polymer brushes …


A Microfluidic Mixer Utilizing Electrokinetic Relay Switching And Asymmetric Flow Geometries, Yiou Wang, Jiang Zhe, Prashanta Dutta, Benjamin Chung Apr 2015

A Microfluidic Mixer Utilizing Electrokinetic Relay Switching And Asymmetric Flow Geometries, Yiou Wang, Jiang Zhe, Prashanta Dutta, Benjamin Chung

Dr. Jiang Zhe

No abstract provided.


High School Bridge Program: A Multidisciplinary Stem Research Program, Jiang Zhe, Dennis Doverspike, Julie Zhao, Paul C. Lam, Craig C. Menzemer Apr 2015

High School Bridge Program: A Multidisciplinary Stem Research Program, Jiang Zhe, Dennis Doverspike, Julie Zhao, Paul C. Lam, Craig C. Menzemer

Dr. Jiang Zhe

A Science, Technology, Engineering and Math (STEM) summer Bridge Program was developed for high school students. The program was designed to encourage students to consider choosing an engineering major in college and to explore STEM as a future career. This was accomplished through a 10-week program involving multidisciplinary research activities. The participants in the program included 33 high school students. Among former participants in position to make a choice in terms of attending college, 100% had chosen to continue on in college, and 86% had chosen to major in a STEM area. The results indicated that that the program had …


An Evaluation Of A Stem Program For Middle School Students On Learning Disability Related Ieps, Paul C. Lam, Dennis Doverspike, Julie Zhao, Jiang Zhe, Craig C. Menzemer Apr 2015

An Evaluation Of A Stem Program For Middle School Students On Learning Disability Related Ieps, Paul C. Lam, Dennis Doverspike, Julie Zhao, Jiang Zhe, Craig C. Menzemer

Dr. Jiang Zhe

A year long Science, Technology, Engineering and Math (STEM) program was developed for middle schools students on Individualized Education Programs (IEPs) involving learning disabilities. The workshops were designed to encourage students both on IEPs and not on IEPs to explore STEM as a future career choice by building their knowledge and confidence. The participants in the workshops included 11 students on IEPs and 15 students not on IEPs. Parents also provided feedback regarding their attitudes toward the program. The results indicated that there were increases in student participant knowledge and career interest for both the students not on IEPs and …


Passive Continuous Particle Focusing In A Microchannel With Symmetric Sharp Corner Structures, Liang-Liang Fan, Liang Zhao, Xu-Kun He, Hand Yu, Qing-Yu Wei, Jiang Zhe Apr 2015

Passive Continuous Particle Focusing In A Microchannel With Symmetric Sharp Corner Structures, Liang-Liang Fan, Liang Zhao, Xu-Kun He, Hand Yu, Qing-Yu Wei, Jiang Zhe

Dr. Jiang Zhe

We report a continuous passive particle focusing method using a novel microchannel with symmetric sharp corners which induce curved streamlines and large centrifugal force on particles. At appropriate flow rate, the centrifugal force generated on particles exceeds the inertial lift force; particles driven by the centrifugal force migrate toward the center of the microchannel, achieving continuous particle focus-ing. With simple structure and operation, this method can be potentially used in particle focusing and ex-traction processes in a variety of lab-on-a chip applications.


A Low-Voltage Droplet Microgripper For Micro-Object Manipulation, Abhay Vasudev, Ashish Jagtiani, Li Du, Jiang Zhe Apr 2015

A Low-Voltage Droplet Microgripper For Micro-Object Manipulation, Abhay Vasudev, Ashish Jagtiani, Li Du, Jiang Zhe

Dr. Jiang Zhe

We present a low-voltage microgripper utilizing a liquid droplet to pick up and release micro-objects. Lifting force is generated by a liquid bridge formed between the gripper surface and an object. Electrowetting was used to dynamically change the capillary lifting forces and enable easy object release. The driving voltage was applied to a pair of coplanar interdigitated electrodes, eliminating the need for an electrode on top of the droplet and thus significantly facilitating object manipulation. A barium strontium titanate insulation layer was used to lower the driving voltage. Experiments indicated that the lifting forces can be as high as 213 …


Inductive Coulter Counting: Detection And Differentiation Of Metal Wear Particles In Lubricant, Li Du, Jiang Zhe, Robert Veillette Apr 2015

Inductive Coulter Counting: Detection And Differentiation Of Metal Wear Particles In Lubricant, Li Du, Jiang Zhe, Robert Veillette

Dr. Jiang Zhe

A device based on an inductive Coulter counting principle for detecting metal particles in lubrication oil is presented. The device detects the passage of ferrous and nonferrous particles by monitoring the inductance change in a coil. First, the sensing principle is demonstrated at the mesoscale using a solenoid. Next, a small planar coil suitable for use in a microscale device is tested. Static tests are conducted on the planar coil using iron and aluminum particles ranging from 80 to 500 µm. The testing results show that the coil can be used to detect and distinguish ferrous and nonferrous metal particles …


Recent Advances In Particle And Droplet Manipulation For Lab-On-A-Chip Devices Based On Surface Acoustic Wave, Zhuochen Wang, Jiang Zhe Apr 2015

Recent Advances In Particle And Droplet Manipulation For Lab-On-A-Chip Devices Based On Surface Acoustic Wave, Zhuochen Wang, Jiang Zhe

Dr. Jiang Zhe

Manipulation of microscale particles and fluid liquid droplets is an important task for lab-on-a-chip devices for numerous biological researches and applications, such as cell detection and tissue engineering. Particle manipulation techniques based on surface acoustic waves (SAWs) appear effective for lab-on-a-chip devices because they are non-invasive, compatible with soft lithography micromachining, have high energy density, and work for nearly any type of microscale particles. Here we review the most recent research and development of the past two years in SAW based particle and liquid droplet manipulation for lab-on-a-chip devices including particle focusing and separation, particle alignment and patterning, particle directing, …


A Rapid Magnetic Particle Driven Micromixer, Yiou Wang, Jiang Zhe, Benjamin Chung, Prashanta Dutta Apr 2015

A Rapid Magnetic Particle Driven Micromixer, Yiou Wang, Jiang Zhe, Benjamin Chung, Prashanta Dutta

Dr. Jiang Zhe

Performances of a magnetic particle driven micromixer are predicted numerically. This micromixer takes advantages of mixing enhancements induced by alternating actuation of magnetic particles suspended in the fluid. Effects of magnetic actuation force, switching frequency and channel’s lateral dimension have been investigated. Numerical results show that the magnetic particle actuation at an appropriate frequency causes effective mixing and the optimum switching frequency depends on the channel’s lateral dimension and the applied magnetic force. The maximum efficiency is obtained at a relatively high operating frequency for large magnetic actuation forces and narrow microchannels. If the magnetic particles are actuated with a …


A Thermal Management Device For A Lower-Limb Prosthesis, Yu Han, Fan Liu, Garrett Dowd, Jiang Zhe Apr 2015

A Thermal Management Device For A Lower-Limb Prosthesis, Yu Han, Fan Liu, Garrett Dowd, Jiang Zhe

Dr. Jiang Zhe

Excessive heat and the resulted perspiration within a prosthetic socket were the most common causes for reporting a reduced quality of life for prosthesis users. This paper presents a novel thermal management device to maintain a constant skin temperature for a lower-limb prosthesis. To decrease the prosthetic socket's thermal resistance, heat pipes were used to concentrate heat flux from the simulated skin surface to a cooling region on the outer surface of the socket where a compact heat sink was attached. A small fan was used to convect heat from the heat sink to the ambient surroundings. Thermal resistance of …


Effects Of Sea Surface Temperature, Cloud Radiative And Microphysical Processes, And Diurnal Variations On Rainfall In Equilibrium Cloud-Resolving Model Simulations, Jiang Zhe, Li Fan, Zhou Shu, Gao Ting Apr 2015

Effects Of Sea Surface Temperature, Cloud Radiative And Microphysical Processes, And Diurnal Variations On Rainfall In Equilibrium Cloud-Resolving Model Simulations, Jiang Zhe, Li Fan, Zhou Shu, Gao Ting

Dr. Jiang Zhe

No abstract provided.


Thermal And Fluid Field Simulation Of Single Pulse Discharge In Dry Edm, T. Wang, Jiang Zhe, Y. Zhang, Y. Li, X. Wen Apr 2015

Thermal And Fluid Field Simulation Of Single Pulse Discharge In Dry Edm, T. Wang, Jiang Zhe, Y. Zhang, Y. Li, X. Wen

Dr. Jiang Zhe

Dry Wire Electrical Discharge Machining (WEDM) is a newly developed technology that uses gases as dielectric and is in favor of environmental protection. Dry finishing of WEDM offers advantages such as better straightness, lower surface roughness and shorter gap length. Experimental studies show that the machining behaviors in dry conditions are quite different from those in conventional liquid dielectric. In order to explore the processing mechanism of dry WEDM, this paper studies the single pulse discharge mechanism in gas with finite element method. In order to obtain the crater morphology with edge raised, the fluid field was simulated by ANAYS …


High Throughput Microfluidic Electrical Impedance Flow Cytometry For Assay Of Micro Particles, Ashish Jagtiani, Jiang Zhe Apr 2015

High Throughput Microfluidic Electrical Impedance Flow Cytometry For Assay Of Micro Particles, Ashish Jagtiani, Jiang Zhe

Dr. Jiang Zhe

No abstract provided.


Optimal Design And Simulation Of A Cross-Plane Micro-Thermoelectric Generator, Xiao Zhu, De Li, Ben Liu, Jiang Zhe Apr 2015

Optimal Design And Simulation Of A Cross-Plane Micro-Thermoelectric Generator, Xiao Zhu, De Li, Ben Liu, Jiang Zhe

Dr. Jiang Zhe

No abstract provided.


Effects Of Particle's Off-Axis Position, Shape, Orientation And Entry Position On Resistance Changes Of Micro Coulter Counting Devices, Zhenpeng Qin, Jiang Zhe, Guo-Xiang Wang Apr 2015

Effects Of Particle's Off-Axis Position, Shape, Orientation And Entry Position On Resistance Changes Of Micro Coulter Counting Devices, Zhenpeng Qin, Jiang Zhe, Guo-Xiang Wang

Dr. Jiang Zhe

With the recent advance in micro/nano-fabrication technology, micro Coulter counters have been widely used in detecting and characterizing micro- and nanoscale objects. In this paper, the electrical resistance change during translocation of a non-conducting particle through a channel is studied numerically. The numerical results are validated by proven analytical results available in the literature. The effects of particle's off-axis position, shape and orientation, and entry position are studied for particles with a large dynamic range. From the numerical results, a new fitted correlation is proposed that can accurately predict the resistance change caused by off-axis spherical particles regardless of their …


High-Throughput, Single-Stream Microparticle Focusing Using A Microchannel With Asymmetric Sharp Corners, Liang-Liang Fan, Yu Han, Xu-Kun He, Liang Zhao, Jiang Zhe Apr 2015

High-Throughput, Single-Stream Microparticle Focusing Using A Microchannel With Asymmetric Sharp Corners, Liang-Liang Fan, Yu Han, Xu-Kun He, Liang Zhao, Jiang Zhe

Dr. Jiang Zhe

A new microfluidic device for fast and high-throughput particle focusing is reported. The particle focusing is based on the combination of inertial lift force effect and centrifugal force effect generated in a microchannel with a series of repeated asymmetric sharp corners on one side of the channel wall. The inertial lift force induces two focused particle streams in the microchannel, and the centrifugal force generated at the sharp corner structures tends to drive the particles laterally away from the corner. With the use of a series of repeated asymmetric sharp corner structures, a single and highly focused particle stream was …


Continuous 3d Particle Focusing In A Microchannel With Curved And Symmetric Sharp Corner Structures, Liang-Liang Fan, Xu-Kun He, Yu Han, Jiang Zhe, Liang Zhao Apr 2015

Continuous 3d Particle Focusing In A Microchannel With Curved And Symmetric Sharp Corner Structures, Liang-Liang Fan, Xu-Kun He, Yu Han, Jiang Zhe, Liang Zhao

Dr. Jiang Zhe

No abstract provided.


Sheathless Size-Based Acoustic Particle Separation, Rasim Guldiken, Myeong Jo, Nathan Gallant, Utkan Demirci, Jiang Zhe Apr 2015

Sheathless Size-Based Acoustic Particle Separation, Rasim Guldiken, Myeong Jo, Nathan Gallant, Utkan Demirci, Jiang Zhe

Dr. Jiang Zhe

Particle separation is of great interest in many biological and biomedical applications. Flow-based methods have been used to sort particles and cells. However, the main challenge with flow based particle separation systems is the need for a sheath flow for successful operation. Existence of the sheath liquid dilutes the analyte, necessitates precise flow control between sample and sheath flow, requires a complicated design to create sheath flow and separation efficiency depends on the sheath liquid composition. In this paper, we present a microfluidic platform for sheathless particle separation using standing surface acoustic waves. In this platform, particles are first lined …


A Versatile Microparticle-Based Immunoaggregation Assay For Macromolecular Biomarker Detection And Quantification, Haiyan Wu, Yu Han, George G. Chase, Qiong Tang, Chen-Jung Lee, Bin Cao, Jiang Zhe, Gang Cheng Apr 2015

A Versatile Microparticle-Based Immunoaggregation Assay For Macromolecular Biomarker Detection And Quantification, Haiyan Wu, Yu Han, George G. Chase, Qiong Tang, Chen-Jung Lee, Bin Cao, Jiang Zhe, Gang Cheng

Dr. Jiang Zhe

The rapid, sensitive and low-cost detection of macromolecular biomarkers is critical in clinical diagnostics, environmental monitoring, research, etc. Conventional assay methods usually require bulky, expensive and designated instruments and relative long assay time. For hospitals and laboratories that lack immediate access to analytical instruments, fast and low-cost assay methods for the detection of macromolecular biomarkers are urgently needed. In this work, we developed a versatile microparticle (MP)-based immunoaggregation method for the detection and quantification of macromolecular biomarkers. Antibodies (Abs) were firstly conjugated to MP through streptavidin-biotin interaction; the addition of macromolecular biomarkers caused the aggregation of Ab-MPs, which were subsequently …