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Inference Space Choices In Combined Mixed Model Analyses Of Discrete Grazing Trials, H A. Fribourg, R W. Thompson, J C. Waller, W L. Sanders Aug 2024

Inference Space Choices In Combined Mixed Model Analyses Of Discrete Grazing Trials, H A. Fribourg, R W. Thompson, J C. Waller, W L. Sanders

IGC Proceedings (1993-2023)

Grazing trials are limited by financial and logistical constraints that restrict duration and number of treatments to be investigated. Combining data from independent trials may be a feasible way to circumvent these constraints and provide a more meaningful body of information. Datasets were pooled from 12 independent trials conducted during the last 13 springs at 9 locations in 7 USA eastern states (a total of 37 location/ years) to provide combined estimates of steer (Bos taurus) performance on Festuca arundinacea pastures free of or infested with the endophytic fungus Acrenoium coenophialium, and to demonstrate the effectiveness and …


An Expert System To Advise Tasmanian Farmers On Pasture Mixtures And Fodder Crops, Peter Gillard Aug 2024

An Expert System To Advise Tasmanian Farmers On Pasture Mixtures And Fodder Crops, Peter Gillard

IGC Proceedings (1993-2023)

In Tasmania, the Department of Primary Industry and Fisheries needed to find a more effective method of informing farmers obout the best pasture and forage species. PASTURBPAK, an expert system, was designed to meet this need. The program offers advice on mixtures of species and lists cultivars that are adapted to the environment, and the need of the farmer and livestock type. Seed merchants have installed the software to make the information available when farmers buy seed. Sales starf can now instantly access the corporate expertise of DPIF, and offer customers printed advice specific to their need. The relevance of …


Comparison Of Two Calibration Procedures For Predicting Leaf Percentages From Forage Sorghums And Pearlmillets Using Near-Infrared Reflectance Spectroscopy, T P. Karnezos, A G. Matches, J W. Keeling Aug 2024

Comparison Of Two Calibration Procedures For Predicting Leaf Percentages From Forage Sorghums And Pearlmillets Using Near-Infrared Reflectance Spectroscopy, T P. Karnezos, A G. Matches, J W. Keeling

IGC Proceedings (1993-2023)

A rapid method for determining leaf percentage (LP) from forage sorghums (Sorglium bicolor (L.) Moench) and pearlmillets (Pennisetum americaum (L.) Leeke) is reported. Our objective was to develop and compare two near-infrared reflectance spectroscopy (NIRS) calibration methods for predicting LP. Calibration methods developed were: I) leaves assigned a value of 99.99% leaf and 0.01 % stem, and stems a value of 99.99% stem and 0.01% leaf; and 2) ground leaf and stem samples mixed by weight to obtain a range of LP. Samples were scanned using NIRS and calibration equations developed. Equations had coefficients of multiple determination (R2) …


Computer Simulation To Aid The Development, Transfer An Adoption Of Alternative Forage Conservation Systems, C A. Rotz, D R. Buckmaster Aug 2024

Computer Simulation To Aid The Development, Transfer An Adoption Of Alternative Forage Conservation Systems, C A. Rotz, D R. Buckmaster

IGC Proceedings (1993-2023)

A computer simulation model of forage production, conservation and use was developed to evaluate forage technologies. The model simulates forage losses and quality changes along with the use and costs of machines, structures, labour, fuel, chemicals, etc. Alfalfa and corn produced on the farm arc supplemented with purchased feeds to feed a dairy herd or they are sold. Simulation over many years of weather conditions provides a long-term evaluation of the performance and economics of alternative forage systems. Simulation results provide manufacturers with design-performance constraints which aid the development of new products. Adoption of available forage technology is aided by …


Developing Long-Term Weather Records For Use With Rangeland Management Modelsc, Clayton L. Hanson, J Ross Wight Aug 2024

Developing Long-Term Weather Records For Use With Rangeland Management Modelsc, Clayton L. Hanson, J Ross Wight

IGC Proceedings (1993-2023)

The use of natural resource models for research and management of rangeland ecosystems is limited by the unavailability of long-term weather records. This paper identifies sources of weather information for the United States; suggests methods for estimating missing records; and illustrates the use of double-mass curve procedures for evaluating the quality of data sets. Where weather data are limited in ducat ion or unavailable, stochastic weather generation is an option to simulate daily weather records.


Making Models Easy To Use, J Ross Wight, Clayton L. Hanson Aug 2024

Making Models Easy To Use, J Ross Wight, Clayton L. Hanson

IGC Proceedings (1993-2023)

A major limitation to the application of natural resource models to resource management problems is the unavailability of input information and complexity of model operation and output interpretation. These problems can be moderated by using synthetic weather data, algorithms and expert systems to calculate model parameters and input variables and computer programs to simplify development of input data files and interpretation of output results. This paper summarises some of the procedures and techniques that can be used to make models easier to use and more useful as management tools.


Simulation Models As Decision Aids For Management Of Rangeland Ecosystems, J Ross Wight, Clayton L. Hanson Aug 2024

Simulation Models As Decision Aids For Management Of Rangeland Ecosystems, J Ross Wight, Clayton L. Hanson

IGC Proceedings (1993-2023)

Rangeland simulation models have been used primarily as research tools to synthesise and organise information and to conceptualise and describe the interacting components of the ecosystem. Their application as decision aids for range management has not been developed. This paper discusses ways simulation models, such as SPUR· (Simulation of Production and Utilisation of Rangelands), can be used to forecast herbage p1oduction and the occurrence of biological windows, enhance monitoring, and predict the impacts of management and climate change on the soil, water, and plant resources including species composition.


Models Of Pasture Production To Aid On-Farm Decision Making In New Zealand, J A. Baars, M D. Rollo Aug 2024

Models Of Pasture Production To Aid On-Farm Decision Making In New Zealand, J A. Baars, M D. Rollo

IGC Proceedings (1993-2023)

Two microcomputer-based predictive pasture models, for use in decision making by agricultural consultants and industry, are described. The first model (GRASS) was developed to understand the interactions between environment and management. The second model supplies pasture growth rates for current or predicted weather conditions by adjustment of stored actual average fortnightly pasture growth rates for a wide range of measurement sites. Ways are described in which the models are being used in transfer of technology.


Production Of Dryland Lucerne In The Western And Southern Cape Regions Of South Africa, J M. Van Heerden Aug 2024

Production Of Dryland Lucerne In The Western And Southern Cape Regions Of South Africa, J M. Van Heerden

IGC Proceedings (1993-2023)

Cutting trials were conducted with lucerne (Medicago sativa) cv. S A Standard under dryland conditions over a period of 8 (1982 to 1989) years at 2 sites, one each in the western (Elsenburg) and southern (Tygerhoek) Cape. The sites differed in soil type and rainfall distribution. Replicated plots were harvested at 3 set frequencies (4·, 6- and 8- weekly). Production was lowest during summer and highest in late spring and increased with decreased harvesting frequency at both sites. These data were used to derive equations for the extrapolation of the seasonal yield. Inputs into the model 11cc average …


Graze Model: Importance Of Simulating Conceptual Grazing Areas Within A Paddock, Otto J. Loewer, L D. Parsch, H D. Scott, C P. West, A L. Goetsch Aug 2024

Graze Model: Importance Of Simulating Conceptual Grazing Areas Within A Paddock, Otto J. Loewer, L D. Parsch, H D. Scott, C P. West, A L. Goetsch

IGC Proceedings (1993-2023)

The selective grazing logic within the computer simulation model GRAZE was tested to determine if predicted ·results differed appreciably from situations ·where non-selective grazing logic· was used. Results indicated that predictions would_ differ somewhat." The main cause of the differences was determined to be the simulated levels of plant dry matter availability, which were considerably higher when non-selective· grazing was used.


Development Of An Expert System For New Zealand Seasonal-Supply Dairy Farmers, D I. Gray, G A. Lynch, E G. Todd, J C. Lockhart, W J. Parker Aug 2024

Development Of An Expert System For New Zealand Seasonal-Supply Dairy Farmers, D I. Gray, G A. Lynch, E G. Todd, J C. Lockhart, W J. Parker

IGC Proceedings (1993-2023)

The mechod for developing an expert system for the drying-off decision on seasonal-supply dairy farms is described. The decision-making process ' of 4 expert seasonal-supply dairy farmers and the consultancy process used by 2 New Zealand Dairy Board Consulting Officers were identified in a series e>f interviews. This information ,was used to develop 2 models; one of expertise and the other of user requirements. These models form the basis of an expert system to improve the decision-making ability of. New Zealand seasonal-supply dairy farmers.


Rehabilitation On Public Rangelands In Idaho, Usa: A Change In Emphasis From Grass Monocultures, Mike Pellant, Stephen B. Monsen Aug 2024

Rehabilitation On Public Rangelands In Idaho, Usa: A Change In Emphasis From Grass Monocultures, Mike Pellant, Stephen B. Monsen

IGC Proceedings (1993-2023)

Since the 1940s the Bureau of Land Management has reseeded 485 830 ha of public rangelands in southern Idaho. Introduced wheatgrasses were seeded on most rehabilitation projects, often resulting in monoculture plant communities. Introduced wheatgrasses were relatively inexpensive to seed, established well, excluded weedy annuals and provided additional livestock forage. Concern about extensive shrubland losses after wildfires prompted BLM managers to begin reseeding native shrubs in the early 1980s. Use of different herbaceous plants on rehabilitation projects is increasing. Seed mixtures now include up to 10 species (grass, forb and shrubs) that, when established, meet multiple use resource objectives.


Micro-Computer Version Of The Database On Feed Commodities Derived From Pastures And Green Crops, H T. Ostrowski-Meissner Aug 2024

Micro-Computer Version Of The Database On Feed Commodities Derived From Pastures And Green Crops, H T. Ostrowski-Meissner

IGC Proceedings (1993-2023)

The subunit of the national feed database devoted specifically to feeds derived from grasslands and green crops is presented. This collection of chemical and nutritional data is the result of 20 years of data collection from analyses conducted from 62 laboratories Australia-wide, The data entries are classified according to each Australian state and laboratory which provided the analytical and nutritional characteristics of fodder. Each entry is classified according to the International Feed Numbering (IFN) system as maintained by the International Network of Feed Information Centres (INFIC), The data in the collection as presented in the paper are an integral part …


A Computer Model For Determining When To Start Spring Grazing, Dennis R. Buckmaster, Warren J. Parker Aug 2024

A Computer Model For Determining When To Start Spring Grazing, Dennis R. Buckmaster, Warren J. Parker

IGC Proceedings (1993-2023)

A simple computer model is presented for determining the earliest grazing date after winter freezing. The model also illustrates principles of rotational grazing management and projects when forage should be conserved during the spring. Inputs to the model include initial herbage cover, herbage growth rate, herbage quality, a post•grazing residual dry matter target, a harvest set•aside threshold, and a description of the grazing animals.


Comparison Of Methods For Assessing Productivity Of Native Pastures In Queensland, K A. Day, G M. Mckeon, D M. Orr Aug 2024

Comparison Of Methods For Assessing Productivity Of Native Pastures In Queensland, K A. Day, G M. Mckeon, D M. Orr

IGC Proceedings (1993-2023)

The relationship between seasonal climatic conditions and observed seasonal pasture growth was examined for 3 native pasture sites in south-east Queensland for periods of up to 5 years. Nine indices of seasonal climatic conditions were considered. Across sites, between year differences in observed growth were best accounted for by the average daily ratio of calculated transpiration and vapour pressure deficit, This index accounted for 83% of the between year variation in observed growl h.


Application Of Neural Networks To The Extraction Of Various Types Of Grasslands In Japan Using Landsat Thematic Mapper Data, Mikinori Tsuiki, Shigeo Takahashi, Toshiki Oku Aug 2024

Application Of Neural Networks To The Extraction Of Various Types Of Grasslands In Japan Using Landsat Thematic Mapper Data, Mikinori Tsuiki, Shigeo Takahashi, Toshiki Oku

IGC Proceedings (1993-2023)

A neural network was applied to the extraction of various types of grasslands using Landsat Thematic Mapper (TM) data. Training fields contained 12 classes (water, paddy field, farmland, sands and rocks, urban area, coniferous forest, deciduous forest, golf course, Sasa­type grassland, Miscanthus type grassland, meadow before culling and meadow after cutting). Classification performance using the neural network was 99.4%, which was 2.4% higher than that obtained using the maximum likelihood method. For all types of grasslands, classification performance was 99.8%. The results of the classification area obtained using the neural network and the maximum likelihood method resembled each other. …


Use Of A Simple Model Of Continuous And Rotational Grazing To Compare Herbage Consumption, Simon J.R Woodward, G `C Wake, D G. Mccall, A B. Pleasants Aug 2024

Use Of A Simple Model Of Continuous And Rotational Grazing To Compare Herbage Consumption, Simon J.R Woodward, G `C Wake, D G. Mccall, A B. Pleasants

IGC Proceedings (1993-2023)

A simple mathematical model (a linear dynamical system) was constructed to predict herbage consumption by grazing animals. This model was used to compare 3 grazing methods over a range of stocking rates at low pasture mass. The grazing methods compared were: continuous grazing (CTS), rotational grazing with equal time intervals (BQ), and rotational grazing where the time of shift was optimal for maximising intake (OPT). The rotational methods were the simplest possible, the land being subdivided into 2 fields, each of which was grazed once. The order of grazing the fields was also considered. The model predicts (I) that at …


Pasture Plant Identification In The Western Queensland Arid Zone, J A. Milson, I F. Beale, J A. Reynolds, J J. Bushwell, D Phelps Aug 2024

Pasture Plant Identification In The Western Queensland Arid Zone, J A. Milson, I F. Beale, J A. Reynolds, J J. Bushwell, D Phelps

IGC Proceedings (1993-2023)

Since plant species composition is an important indicator of the health or condition of pastures and therefore the land, a first step for graziers interested in conserving the total range resource is to understand vegetation species and thus be able to identify them and to recognise species changes. This project is aimed at producing and distributing colour leaflets of the major native and problem plant species (including woody weeds) in the four major community types in western Queensland. The number of colour leaflels for the series totals 60. The number of species represented is about 230. These leaflets are dislributed …


Farm Monitoring, A Way To Improve Performance On Sheep And Beef Farms, R W. Webby, G W. Sheath Aug 2024

Farm Monitoring, A Way To Improve Performance On Sheep And Beef Farms, R W. Webby, G W. Sheath

IGC Proceedings (1993-2023)

A programme that centres on monitoring the performance of sheep and beef farms is being used to encourage farmers to make decisions based on objective measurements, Monitor farmers are supported by their farming peers in groups that include extension and science personnel. Key biological performance indicators such as animal numbers, weights; weight gains, pasture production; and financial returns from meat and wool are monitored. Farmers are encouraged to be in a position to use this information and identify opportunities. Processors of sheep and beef products are demanding the production of livestock to tighter specifications. Close monitoring by farmers will be …


Use Of A Model To Investigate The Profitability Of Fertilizer Application, T.J M. Johnston, D G. Mccall, P R. Marshall Aug 2024

Use Of A Model To Investigate The Profitability Of Fertilizer Application, T.J M. Johnston, D G. Mccall, P R. Marshall

IGC Proceedings (1993-2023)

Use of a model to investigate the payback to alternative phosphate (P) fertiliser programmes is described. Input for the model is fertiliser history (kg P/ho/year) over 5-15 years. A site fertility index is established by summing discounted P applications. The index is related to relative pasture yield (annual yield/non P limited yield) for a soil type. The economics of P use are evaluated by scaling current gross margin/ha for changes in relative pasture yield and subtracting fertiliser costs. The model provides the decision maker with yearly cashflow information and a I 0-year net present value (NPV) analysis of the relative …


Meti: Deep Profiling Of Tumor Ecosystems By Integrating Cell Morphology And Spatial Transcriptomics, Jiahui Jiang, Yunhe Liu, Jiangjiang Qin, Jianfeng Chen, Jingjing Wu, Melissa P Pizzi, Rossana Lazcano, Kohei Yamashita, Zhiyuan Xu, Guangsheng Pei, Kyung Serk Cho, Yanshuo Chu, Ansam Sinjab, Fuduan Peng, Xinmiao Yan, Guangchun Han, Ruiping Wang, Enyu Dai, Yibo Dai, Bogdan A Czerniak, Andrew Futreal, Anirban Maitra, Alexander Lazar, Humam Kadara, Amir A Jazaeri, Xiangdong Cheng, Jaffer Ajani, Jianjun Gao, Jian Hu, Linghua Wang Aug 2024

Meti: Deep Profiling Of Tumor Ecosystems By Integrating Cell Morphology And Spatial Transcriptomics, Jiahui Jiang, Yunhe Liu, Jiangjiang Qin, Jianfeng Chen, Jingjing Wu, Melissa P Pizzi, Rossana Lazcano, Kohei Yamashita, Zhiyuan Xu, Guangsheng Pei, Kyung Serk Cho, Yanshuo Chu, Ansam Sinjab, Fuduan Peng, Xinmiao Yan, Guangchun Han, Ruiping Wang, Enyu Dai, Yibo Dai, Bogdan A Czerniak, Andrew Futreal, Anirban Maitra, Alexander Lazar, Humam Kadara, Amir A Jazaeri, Xiangdong Cheng, Jaffer Ajani, Jianjun Gao, Jian Hu, Linghua Wang

Student and Faculty Publications

Recent advances in spatial transcriptomics (ST) techniques provide valuable insights into cellular interactions within the tumor microenvironment (TME). However, most analytical tools lack consideration of histological features and rely on matched single-cell RNA sequencing data, limiting their effectiveness in TME studies. To address this, we introduce the Morphology-Enhanced Spatial Transcriptome Analysis Integrator (METI), an end-to-end framework that maps cancer cells and TME components, stratifies cell types and states, and analyzes cell co-localization. By integrating spatial transcriptomics, cell morphology, and curated gene signatures, METI enhances our understanding of the molecular landscape and cellular interactions within the tissue. We evaluate the performance …


Microeukaryote Metabolism Across The Western North Atlantic Ocean Revealed Through Autonomous Underwater Profiling, Natalie R. Cohen, Arianna I. Krinos, Riss M. Kell, Rebecca J. Chmiel, Dawn M. Moran, Matthew R. Mcilvin, Paloma Z. Lopez, Brianna A. Alanis, Eric W. Chan, John A. Breier Aug 2024

Microeukaryote Metabolism Across The Western North Atlantic Ocean Revealed Through Autonomous Underwater Profiling, Natalie R. Cohen, Arianna I. Krinos, Riss M. Kell, Rebecca J. Chmiel, Dawn M. Moran, Matthew R. Mcilvin, Paloma Z. Lopez, Brianna A. Alanis, Eric W. Chan, John A. Breier

School of Earth, Environmental, and Marine Sciences Faculty Publications and Presentations

Microeukaryotes are key contributors to marine carbon cycling. Their physiology, ecology, and interactions with the chemical environment are poorly understood in offshore ecosystems, and especially in the deep ocean. Using the Autonomous Underwater Vehicle Clio, microbial communities along a 1050 km transect in the western North Atlantic Ocean were surveyed at 10–200 m vertical depth increments to capture metabolic signatures spanning oligotrophic, continental margin, and productive coastal ecosystems. Microeukaryotes were examined using a paired metatranscriptomic and metaproteomic approach. Here we show a diverse surface assemblage consisting of stramenopiles, dinoflagellates and ciliates represented in both the transcript and protein fractions, …


Division I Collegiate Baseball Pitching Performance In Relation To Forearm Kinematics And Kinetics, Christopher W. Watson Aug 2024

Division I Collegiate Baseball Pitching Performance In Relation To Forearm Kinematics And Kinetics, Christopher W. Watson

Master's Theses

This thesis investigates physical and anthropometric factors influenced by forearm rotation biases associated with supination and pronation on pitch characteristics among Division I college baseball pitchers. The study involved twenty-two participants who underwent comprehensive physical assessments, including measures of forearm strength, range of motion, and grip strength, as well as finger and hand length measurements. Ball flight metrics were collected during intrasquad scrimmages using advanced radar tracking technology. Results revealed significant relationships between distinct physical biases of strength in pronation and supination, pinch strength, anthropometrics such as middle finger length, and specific pitch characteristics. This study lays the groundwork for …


Slow Ramping Emerges From Spontaneous Fluctuations In Spiking Neural Networks, Jake Gavenas, Ueli Rutishauer, Aaron Schurger, Uri Maoz Aug 2024

Slow Ramping Emerges From Spontaneous Fluctuations In Spiking Neural Networks, Jake Gavenas, Ueli Rutishauer, Aaron Schurger, Uri Maoz

Psychology Faculty Articles and Research

The capacity to initiate actions endogenously is critical for goal-directed behavior. Spontaneous voluntary actions are typically preceded by slow-ramping activity in medial frontal cortex that begins around two seconds before movement, which may reflect spontaneous fluctuations that influence action timing. However, the mechanisms by which these slow ramping signals emerge from single-neuron and network dynamics remain poorly understood. Here, we developed a spiking neural-network model that produces spontaneous slow ramping activity in single neurons and population activity with onsets ~2 s before threshold crossings. A key prediction of our model is that neurons that ramp together have correlated firing patterns …


Prostate Tissue Motion Tracking And Ultrasound Elastography Using Tissue Mechanics Derived Constraints, Tristan S. Curry Aug 2024

Prostate Tissue Motion Tracking And Ultrasound Elastography Using Tissue Mechanics Derived Constraints, Tristan S. Curry

Electronic Thesis and Dissertation Repository

Current prostate cancer detection methods can be costly to obtain, such as magnetic resonance imaging, or lack specificity, such as a digital rectal exam. Ultrasound elastography, a method that can be used to develop and test algorithms that output stiffness, strain, and displacement data captured by ultrasound radio frequency readings, offers a potential solution to these challenges. An initial algorithm utilizing dynamic programming and analytic minimization estimates the radial and angular displacements from a pre- and post-compression data set to determine the required material properties. This estimate of displacements is then refined through an algorithm where incompressibility, Laplacian smoothing, and …


Turkey Tail Mushroom (Trametes Versicolor): An Edible Macrofungi With Immense Medicinal Properties, Olaide Olaide Ajibola, Cirilo Nolasco-Hipolito, Octavio Carvajal-Zarrabal, Shanti F. Salleh, Gbadebo C. Adeyinka, Stephen A. Adefegha, Mirja K. Ahmmed, Kazi Sumaiya, Raymond Thomas Aug 2024

Turkey Tail Mushroom (Trametes Versicolor): An Edible Macrofungi With Immense Medicinal Properties, Olaide Olaide Ajibola, Cirilo Nolasco-Hipolito, Octavio Carvajal-Zarrabal, Shanti F. Salleh, Gbadebo C. Adeyinka, Stephen A. Adefegha, Mirja K. Ahmmed, Kazi Sumaiya, Raymond Thomas

Biology Publications

Macrofungi, commonly known as mushrooms, are not only considered functional foods for supplying essential nutritional ingredients but also a good source of physiologically beneficial medicines. Trametes versicolor, referred to as turkey tail’s Mushroom or Yun Zhi in China, is an edible mushroom that has extensive historical usage in conventional and traditional Chinese medicine. This mushroom contains an abundance of physiologically bioactive compounds, most notably β-glucan polysaccharides, which are responsible for antioxidant, neuroprotection, hypolipidemic effects, immune-modulating effects, and anticancer effects. Trametes versicolor has also been revealed to have wound healing, antidiabetic, antimicrobial, antifibrotic, neurotrophic, and …


First Serological Evidence Of Coxiella Burnetii And Mycobacterium Avium Subspecies Paratuberculosis (Map) Among Deer In An Institutional Farm In Malaysia, Bura Thlama Paul, Faez Firdaus Abdullah Jesse, Shivnraj Sundar Universiti Putra Malaysia, Nur Hanis Nadhirah Yosryhan, Yonis Ahmed Ahmed Jimale Dr, Eric Lim Teik Chung Dr, Mohd Azmi Mohd Lila Prof Aug 2024

First Serological Evidence Of Coxiella Burnetii And Mycobacterium Avium Subspecies Paratuberculosis (Map) Among Deer In An Institutional Farm In Malaysia, Bura Thlama Paul, Faez Firdaus Abdullah Jesse, Shivnraj Sundar Universiti Putra Malaysia, Nur Hanis Nadhirah Yosryhan, Yonis Ahmed Ahmed Jimale Dr, Eric Lim Teik Chung Dr, Mohd Azmi Mohd Lila Prof

The Thai Journal of Veterinary Medicine

Q fever is a zoonotic disease caused by Coxiella burnetii, which is associated with reproductive disorders such as abortion and reproductive problems in ruminants, while paratuberculosis is an underdiagnosed disease caused by Mycobacterium avium paratuberculosis (MAP) which causes chronic wasting, low fertility, and mortality in ruminants. Although both C. burnetii and MAP infections have been reported in domestic ruminants worldwide, there is no data on their seroprevalence status among deer in Selangor, Malaysia. This study investigated the seroprevalence status of C. burnetii and MAP among deer in Malaysia. Ninety-two blood sera collected from an institutional deer farm were screened …


Multidrug-Resistant Esbl-Producing Escherichia Coli Coexisting With Colistin-Resistance Genes In Pig Farms, Central Thailand, Wipawee Songsaeng, Nutthee Am-In, Nuvee Prapasarakul, Wandee Sirichokchatchawan Aug 2024

Multidrug-Resistant Esbl-Producing Escherichia Coli Coexisting With Colistin-Resistance Genes In Pig Farms, Central Thailand, Wipawee Songsaeng, Nutthee Am-In, Nuvee Prapasarakul, Wandee Sirichokchatchawan

The Thai Journal of Veterinary Medicine

The presence of extended-spectrum beta-lactamase and colistin resistance E. coli in food-producing animals is a public health concern. This study was conducted using a rigorous methodology to survey the prevalence of ESBL-producing and colistin-resistant E. coli from pig farms in the central part of Thailand. A total of 519 samples were collected from fecal, feed, and waste in 53 farms located in central Thailand. All samples were inoculated onto selective agar, and species identification was performed using biochemical assays and MAlDI-TOF MS. Antimicrobial susceptibility was determined by broth microdilution, and ESBL production was confirmed by the combination disc diffusion method. …


Effect Of Heat Stress And Feed Type On Trimethylamine Concentration And Fishy Odor Of Duck Eggs, Pachara Pearodwong, Winai Kaewlamun, Sarawanee Khunmanee, Thuwanon Boonkerd, Thananon Junsuri, Kajohn Nitiwararak, Pantira Hempattarasuwan, Pattrarat Chanchaithong Aug 2024

Effect Of Heat Stress And Feed Type On Trimethylamine Concentration And Fishy Odor Of Duck Eggs, Pachara Pearodwong, Winai Kaewlamun, Sarawanee Khunmanee, Thuwanon Boonkerd, Thananon Junsuri, Kajohn Nitiwararak, Pantira Hempattarasuwan, Pattrarat Chanchaithong

The Thai Journal of Veterinary Medicine

We investigated the effect of heat stress and feed type on the trimethylamine (TMA) concentration and the fishy odor of duck eggs (Study 1) and the relationship between the TMA level in serum and egg content (Study 2). In Study 1, pooled egg samples (n = 75) were divided by feed type (CP12, n = 24; CP18, n = 27; CP20, n = 24) and heat stress (no, n = 49; moderate, n = 15; severe, n = 11). The collected samples were obtained for TMA analysis and sensory testing. In Study 2, a total of 15 egg and 15 …


The Icf Syndrome Protein Cdca7 Harbors A Unique Dna Binding Domain That Recognizes A Cpg Dyad In The Context Of A Non-B Dna, Swanand Hardikar, Ren Ren, Zhengzhou Ying, Jujun Zhou, John R Horton, Matthew D Bramble, Bin Liu, Yue Lu, Bigang Liu, Luis Della Coletta, Jianjun Shen, Jiameng Dan, Xing Zhang, Xiaodong Cheng, Taiping Chen Aug 2024

The Icf Syndrome Protein Cdca7 Harbors A Unique Dna Binding Domain That Recognizes A Cpg Dyad In The Context Of A Non-B Dna, Swanand Hardikar, Ren Ren, Zhengzhou Ying, Jujun Zhou, John R Horton, Matthew D Bramble, Bin Liu, Yue Lu, Bigang Liu, Luis Della Coletta, Jianjun Shen, Jiameng Dan, Xing Zhang, Xiaodong Cheng, Taiping Chen

Student and Faculty Publications

CDCA7, encoding a protein with a carboxyl-terminal cysteine-rich domain (CRD), is mutated in immunodeficiency, centromeric instability, and facial anomalies (ICF) syndrome, a disease related to hypomethylation of juxtacentromeric satellite DNA. How CDCA7 directs DNA methylation to juxtacentromeric regions is unknown. Here, we show that the CDCA7 CRD adopts a unique zinc-binding structure that recognizes a CpG dyad in a non-B DNA formed by two sequence motifs. CDCA7, but not ICF mutants, preferentially binds the non-B DNA with strand-specific CpG hemi-methylation. The unmethylated sequence motif is highly enriched at centromeres of human chromosomes, whereas the methylated motif is distributed throughout …