Digital Commons Network^™

Acute Ingestion Of Dark Chocolate Fails To Affect Running Economy In Recreationally Trained Female Runners, Bianca De Lucia, Beau K. Greer, Christopher B. Taber

PTHMS Graduate Publications

Ingestion of dark chocolate (DC), a dietary source high in flavanols, may increase nitric oxide bioavailability. Elevating blood nitric oxide concentrations may augment metabolic efficiency by reducing the amount of oxygen or energy needed to perform a given task. Utilizing a crossover design, the purpose of this study was to investigate the effect of acute ingestion of DC on running economy (RE). Nineteen recreationally trained females (age: 20±1 years) volunteered for this investigation, with 16 completing all procedures (n = 16). Two-hours before RE assessment, participants consumed either 42.5 g of DC or an isocaloric amount of white chocolate (WC) …

Using Virus Like Particle Conjugates Of Synthetic Targeting Compounds To Deliver Chemotherapeutic Drugs To Cancer Stem Cells, Austen W. Kerzee

Chemistry Theses

Cancer stem cells are a type of cell that have the properties of both cancer cells and stem cells. They can differentiate into other types of cancer cells, are resistant to conventional chemotherapeutics, and seem to contribute greatly to the metastasis and recurrence of cancer. Due to these properties, eliminating cancer stem cells would be greatly beneficial in the treatment of cancer. While there have been approved therapeutic methods for the removal of a few of the cancer stem cells types, treatment for most types of cancer stem cells are still in the experimental phase and have yet to be …

Assessing Lipid Composition Of Cell Membrane In Escherichia Coli Under Aerobic And Anaerobic Conditions, Isabelle Johnson

Undergraduate Theses, Professional Papers, and Capstone Artifacts

Escherichia coli is a highly studied model organism that is tightly tied to the mammalian gastrointestinal system. This microorganism has the capability to be a beneficial gut microbe or a life-threatening pathogen. In this study, the lipid membrane of Escherichia coli was investigated using Matrix-Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) to observe the change in its composition in aerobic and anaerobic conditions. Evidence of desaturation was discovered in the spectra, though more investigation is needed to understand the metabolic processes and drives that result in this change. Elucidation of these pathways in the future could result in …

Exploring The Impact Of Invasive On Grassland Diversity: A Study In Montana, Ella Gaffney

Undergraduate Theses, Professional Papers, and Capstone Artifacts

For the capstone project with the University of Montana Davidson Honors College, I investigated the diversity index of grassland and rangeland plots across the state of Montana. My study, conducted from June to August 2023, includes data collected by my team. The purpose of this research project was testing the hypothesis that the diversity index decreases in plots containing invasive grass species.

During the summer of 2023, the Spatial Analysis Lab surveyed over 1000 plots in rangelands and grasslands across the state of Montana to locate an invasive grass, Ventenata dubia. Data collected includes species identification, ocular estimates of …

Estimation Of Probability Of Habitat Use Of Roosevelt Elk On The Olympic Peninsula, Vincent Michael Gugliotti

Undergraduate Theses, Professional Papers, and Capstone Artifacts

Estimating the probability of habitat use for a particular species is crucial to the direct management and conservation of that species. Without knowledge of habitat preferences, managers cannot effectively focus efforts on vital resources or landscape types. However, modelling probability of habitat use can be done in several ways which leaves room for variation and uncertainty in the estimates produced by each method. This study is an examination of the variation between two estimates of probability of habitat use while focusing on a particular subspecies of elk that inhabits a unique ecosystem relative to other elk subspecies. I modeled elk …

A Road Map For Place Based Collaboration For Conflict Reduction, Joseph L. Zecher

Undergraduate Theses, Professional Papers, and Capstone Artifacts

No abstract provided.

Creation Of A Digital Storage System For Genome Sequencing Metadata, Jacquelin W. Olexa

Undergraduate Theses, Professional Papers, and Capstone Artifacts

As the field of computational genomics continues to expand in both potential and application, it is now more imperative than ever to ensure that massive genetic sequencing datasets are properly stored in an accessible manner. This project sought to establish a practical, user-friendly, secure system for a genomics research lab (the Good Lab; thegoodlab.org) at the University of Montana. A MySQL database and connected web application was ruled the best configuration to maximize utility and accessibility for the lab’s researchers. Building the logical framework for the database, creating the server, and sourcing data occurred over several months. The dataset ranged …

Calcium And Proteasomal Regulation Of Er-To-Golgi Protein Transport In Neurons, Samuel A. Galindo

Undergraduate Theses, Professional Papers, and Capstone Artifacts

Regulatory mechanisms of protein trafficking from the endoplasmic reticulum (ER) are critical to understand since neurodegenerative diseases involve defects in this process leading to chronic ER stress and cell death. This study aimed to better understand the calcium regulatory mechanisms of ER-to-Golgi trafficking in hybrid neuroglioblastoma cells (NG108). Specifically, we asked whether proteasomal degradation of transport machinery was involved in the previously demonstrated upregulation of ER-to-Golgi transport evoked by calcium signaling. Based upon previous literature, we believe that the calcium induced increase in transport is due to the activation of the calcium sensitive regulatory protein apoptosis linked gene 2 (ALG-2) …

Male Beetle Courtship Dance Analysis By Deeplabcut Technology, Andrea Newbrough

Undergraduate Theses, Professional Papers, and Capstone Artifacts

No abstract provided.

The Bird Family Tree: How All The Major Bird Groups Are Related To Each Other., Eric M. Ray

Undergraduate Theses, Professional Papers, and Capstone Artifacts

This is a video project designed to help inform those who are curious about the shape of the bird family tree. This tree (or phylogeny) covers how the major groups of birds are related to each other. Though not a deep dive into the phylogeny (individual bird families are not covered, for example), this video seeks to give people the basic overview of how groups relate to each other. The total length is just under 30 minutes. At the start the video covers the basics on how to read a phylogeny, and then gets into the discussion of how the …

The Genetic Basis Of Two Reproductive Traits In Monkeyflowers: Stigma Closure And Corolla Carotenoids, Rachel Anne Halperin

Undergraduate Theses, Professional Papers, and Capstone Artifacts

Abstract

The interactions between pollinators and flowers have long been a driving force for the evolution of many physical floral traits. Traits such as flower shape, size, color, and smell are just some of these traits that evolve because of these interactions. This evolution does not only occur in the more obvious morphological floral traits, however, but also in more subtle traits like touch sensitive stigma closure. In hundreds of Lamiales species, the bilobed stigma, the organ that receives pollen from pollinators, closes rapidly upon touch. Theory and experiments show that this novel dynamic reproductive trait increases pollen export and …

A Genus In The Bacterial Phylum Aquificota Appears To Be Endemic To Aotearoa-New Zealand, Jean F. Power, Carlo R. Carere, Holly E. Welford, Daniel T. Hudson, Kevin C. Lee, John W. Moreau, Anna-Louise Reysenbach, Thijs J.G. Ettema, Multiple Additional Authors

Biology Faculty Publications and Presentations

Allopatric speciation has been difficult to examine among microorganisms, with prior reports of endemism restricted to sub-genus level taxa. Previous microbial community analysis via 16S rRNA gene sequencing of 925 geothermal springs from the Taupō Volcanic Zone (TVZ), Aotearoa-New Zealand, revealed widespread distribution and abundance of a single bacterial genus across 686 of these ecosystems (pH 1.2-9.6 and 17.4-99.8 °C). Here, we present evidence to suggest that this genus, Venenivibrio (phylum Aquificota), is endemic to Aotearoa-New Zealand. A specific environmental niche that increases habitat isolation was identified, with maximal read abundance of Venenivibrio occurring at pH 4-6, 50-70 °C, and …

Effects Of Supplementing Corn Silage To Fall-Calving Heifers And Cows Grazing Tall Fescue On Cow Performance, J. Wyatt L. Banks, Jaymelynn K. Farney, Karol E. Fike, Jason M. Warner

Kansas Agricultural Experiment Station Research Reports

The objective was to test the effect of fescue cultivars and supplemental feeding of cows grazing fescue pastures on cow performance and pasture management. Primiparous and multiparous Angus-based crossed cows (n = 45) averaging 151 ± 7 d of gestation at turnout, were randomly allocated and stratified to pasture treatment by parity. Each pasture contained 3 cows of different ages, consisting of a young (first-calf heifer), middle-aged (3-5 yrs.), and old (≥ 6 yrs.) cow. Treatment was a 2 by 2 factorial design with two types of pasture (toxin producing or non-toxin producing fescue) and two levels of supplemental feeding …

Role Of Soil Management In Control Of Soil-Borne Diseases, Gretchen F. Sassenrath, Christopher R. Little, Xiaomao Lin

Kansas Agricultural Experiment Station Research Reports

Soil-borne diseases are a significant cause of crop yield reduction. Alternative soilmanagement methods can enhance the soil’s natural disease-controlling organisms. This study explores the effect of alternative production methods on a primary soybean disease, charcoal rot, caused by the fungus Macrophomina phaseolina. Treatments that could potentially enhance or reduce the disease pressure were implemented, and soil tests were conducted for nutrients, soil properties, and disease presence. Manure increased the nutrient levels in the soil, as expected, but did not influence disease control. Solarization increased the temperature within the plots and increased the number of colony-forming units (CFUs) of M. …

Screening H3 Histone Acetylation In A Wild Bird, The House Sparrow (Passer Domesticus), Daniella Ray

Electronic Theses and Dissertations

Epigenetic mechanisms are increasingly understood to have major impacts across ecology. However, one molecular epigenetic mechanism, DNA methylation, currently dominates the literature. A second mechanism, histone modification, is likely important to ecologically relevant phenotypes and thus warrants investigation, especially because molecular interplay between methylation and histone acetylation can strongly affect gene expression. There are a limited number of histone acetylation studies on non-model organisms, yet those that exist show that it can impact gene expression and phenotypic plasticity. Wild birds provide an excellent system to investigate histone acetylation, as free-living individuals must rapidly adjust to environmental change. Here, we screen …

Predicting Leucotaraxis Spp. Adult Emergence In The Pacific Northwest With Implications For Biological Control Of Hemlock Woolly Adelgid In The Eastern United States, Liam Farley

Graduate College Dissertations and Theses

Hemlock woolly adelgid (Adelges tsugae) is a non-native, invasive pest of eastern hemlock (Tsugae canadensis) and Carolina hemlock (Tsugae caroliniana), both of which are considered important foundation species in forest ecosystems. Adelges tsugae has caused widespread decline and mortality of hemlock trees in eastern North America. Native hemlocks are particularly vulnerable because of the absence of co-evolved plant defenses and lack of natural enemies of A. tsugae. Managing A. tsugae through development of a biological control program has great potential at the regional level. Two species of silver fly from the Pacific northwest (PNW) Leucotaraxis argenticollis and Leucotaraxis piniperda are …

Investigating Division Quest: A Text-Based Adventure Narrative Game For Overcoming Difficulties Of Mitosis Learning, Amanda G. Conner

Electronic Theses and Dissertations

Mitosis learning is a source of struggle for many introductory biology students. Much of this difficulty stems from an overwhelming amount of terminology and moving pieces as well as the abstract nature of cellular processes. Game-based learning (GBL) has been used in other contexts to reduce cognitive load and provide relatable experiences upon which students can construct their mental models. However, commercial GBL is often expensive and difficult to align with individual instructor teaching preferences and learning objectives. In this study, we use a mixed methods approach to explore the use of a GBL tool made in Twine to overcome …

Estrogen Receptor (Er) Alpha Regulatory Mechanisms And Therapeutic Strategies In Er+ Breast Cancer, Bianca A. Romo

Dartmouth College Ph.D Dissertations

Breast cancer is among the most frequently diagnosed cancers in the U.S. and is one of the leading causes of cancer-related mortalities, second to lung cancer. Estrogen receptor alpha-positive (ER+) breast cancer accounts for 2/3 of diagnosed cases. Patients diagnosed with this subtype of breast cancer typically undergo endocrine therapy that aims to mitigate the growth-promoting effects of estrogen/ER. While therapies are effective, 1/3 of patients will experience recurrence. To begin addressing this drug-resistant patient population, we investigated potential drug targets involved in response to treatment.

Coregulators have been implicated in the regulation of ER transcriptional activity and subsequently affecting …

Target Selection And Enhancement During Attentional Tracking, Marvin R. Maechler

Dartmouth College Ph.D Dissertations

At any waking moment, we are bombarded with more sensory information than we can fully process. Attention is necessary to deal with the dynamic world we live in. One fundamental function of vision and attention is to keep track of moving objects, but what are the targets of attention during tracking?

One of the first theories of attentional tracking predicted that targets would be selected at early processing stages. By employing the double-drift illusion, which dissociates physical and perceived positions of moving objects, we investigated which of these positions is selected for tracking. Contrary to earlier theories and in line …

199965, David Kerstetter

PERC Albacore sPAT Data

Datasets (and supporting material) from 4sPAT electronic tags deployed on albacore caught by pelagic longline gear in the western North Atlantic.

199953, David Kerstetter

PERC Albacore sPAT Data

Datasets (and supporting material) from 4sPAT electronic tags deployed on albacore caught by pelagic longline gear in the western North Atlantic.

199949, David Kerstetter

PERC Albacore sPAT Data

Datasets (and supporting material) from 4sPAT electronic tags deployed on albacore caught by pelagic longline gear in the western North Atlantic.

199946, David W. Kerstetter

PERC Albacore sPAT Data

Datasets (and supporting material) from 4sPAT electronic tags deployed on albacore caught by pelagic longline gear in the western North Atlantic.

Late Summer Plankton Community Variation In Near Shore Environments, Aidan Jacobs-Walker

Copley Library Undergraduate Research Awards

Phytoplankton communities have profound impacts on ecosystem health and function. In the Southern California Bight, hydrographic conditions play a key role in determining the composition of phytoplankton communities. This study conducted hydrographic and plankton community analyses of the nearshore environment off Dana Point, California, during late September. Similar to other studies in the SCB during fall, we observed weakly stratified, oligotrophic waters dominated by dinoflagellates under 10 μm. However, the recorded plankton abundances onshore vs. offshore deviated from the “green ribbon” typical of the SCB, in which phytoplankton are much more abundant onshore than offshore. Additionally, while the onshore community …

An Agroecological Approach To Improving Soil Health Practices On Vermont Vegetable Farms, Rebecca Reed Maden

Graduate College Dissertations and Theses

Vermont’s vegetable farms are highly valued for their contributions to the state's food system, environment, and communities, yet their continued success is impeded by many challenges. Specifically, soil is a valued and vulnerable resource, but management of it requires knowledge, money, and time. This dissertation applies an agroecological approach to understanding soil health practices on vegetable farms in three distinct studies.

In the first part of this dissertation, data is collected from six on-farm research trials in 2017-2018 to better understand the nitrogen (N) dynamics following two commonly planted legume-grass cover crop mixes: field peas (Pisum sativum, var. ‘4010’) and …

Characterization Of A Pathological Tau Point Mutation, E342v, Implicated In Frontotemporal Dementia, Liam Clancy

Graduate College Dissertations and Theses

Frontotemporal Dementia (FTD) is classified as a Tauopathy, a group of heterogenous neurodegenerative disorders characterized by the abnormal functioning of a microtubule associated protein (MAP), Tau. Microtubules, an integral cytoskeletal component in neurons, are thought to be stabilized by Tau. In disease states such as FTD, a reduction in binding affinity of Tau for the microtubule is believed to lead to intraneural Tau deposition and aggregation, which subsequently causes microtubule instability. Consequently, neuronal health is compromised and an array of brain functions decline creating the symptomology seen in FTD. However, it is now known that Tau has a myriad of …

The Stereological And Morphometrical Investigation Of Pars Lumbalis Of The Spinal Cord Innew Zealand Rabbit, Berna Duman, İftar Gürbüz

Turkish Journal of Veterinary & Animal Sciences

In the study, 12 (6 females, 6 males) healthy adult New Zealand rabbits were used to examine the morphometrical andstereological values. White matter, gray matter and total segment volume of the segments in the pars lumbalis were calculated byCavalieri’s principle. The findings were grouped according to sexand statistically evaluated. As a result, 38% of the length of the spinalcord in rabbits belonged to the pars lumbalis. The morphometric parameters were not statistically different between male and femalerabbits (p > 0.05). The segment with the biggest volume was L5. White matter’s greatest volume was the L4 segment. The volume of graymatter was …

Upa-Mediated Polyamidoamine Dendrimer-Based Targeted Gene Delivery System On Triple-Negative Breast Cancer, Hsin-Yin Chuang

Masters Theses

"Around 15% of breast cancers are triple-negative breast cancer (TNBC), which is characterized by the absence of three common receptors—ER, PR, and HER2, and therefore do not respond to hormonal or anti-HER2 therapies. It is urgent to explore targeted therapeutic strategies for TNBC due to its poor prognosis and rare effective targeted therapy. In this study, we developed a polyamidoamine (PAMAM) dendrimer-based targeted gene delivery system—GDP-uPA, to utilize urokinase-type plasminogen activator (uPA) to target uPA receptor (uPAR), which is highly expressed in both TNBC cells and cancer-associated stromal cells. Our results of 1H NMR spectrum, TEM imaging, and MTT assay …

Association Between Serum Procalcitonin Level With Exacerbation Severity In Chronic Obstructive Pulmonary Disease Patients, Dalia Abdellateif Abdelghany, Shimaa R. Hendawy, Rania Mostafa, Heba Wagih Abdelwahab

Mansoura Medical Journal

Background: There is a significant impact on morbidity, mortality, and quality of life due to chronic obstructive pulmonary disease exacerbations. Individuals with acute exacerbation of chronic obstructive pulmonary disease had considerably higher mean serum procalcitonin levels (PCT) compared to individuals with stable chronic obstructive pulmonary disease, according to previous investigations. Objective: This study aimed to assess whether procalcitonin levels were associated with the severity of exacerbations in patients with chronic obstructive pulmonary disease. Methods: thirty-nine patients who were suffering from an acute exacerbation of chronic obstructive pulmonary disease were included. Included patients were classified as having either no respiratory failure, …

Innate And Adaptive Immune System Consequences Of Post-Traumatic Stress Disorder, Tatlock H. Lauten, Tamara Natour, Adam J. Case

Journal Articles: Cellular & Integrative Physiology

In the field of psychiatry, biological markers are rarely, if ever, used in the diagnosis of mental health disorders. Clinicians rely primarily on patient histories and behavioral symptoms to identify specific psychopathologies, which makes diagnosis highly subjective. Moreover, therapies for mental health disorders are aimed specifically at attenuating behavioral manifestations, which overlooks the pathophysiological indices of the disease. This is highly evident in posttraumatic stress disorder (PTSD) where inflammation and immune system perturbations are becoming increasingly described. Further, patients with PTSD possess significantly elevated risks of developing comorbid inflammatory diseases such as autoimmune and cardiovascular diseases, which are likely linked …