Digital Commons Network^™

Studies Of The Purification Of Nad-Dependent And Nadp-Dependent Isocitrate Dehydrogenase By General Ligand Affinity Chromatography, Geoffrey H. Bertkau

Chemistry & Biochemistry Theses & Dissertations

Purification of Yeast NAD⁺-dependent isocitrate dehydrogenase was partially successful when utilizing an NAD⁺-ribosyl-hydrazide Sepharose affinity chromatography column. Although no specific elution system was found, a maximum purification of sixty-five fold was achieved with 0.5M KCl elution. A 38-fold purification was obtained when 20mM NAD⁺ was employed for elution.

Yeast and calf liver NADP⁺-dependent isocitrate dehydrogenase were also found to bind to NAD⁺-ribosyl-hydrazide Sepharose as well as the NAD⁺-dependent enzyme. Again, elution was achieved with either o,5M KCl or 20 mM NADP⁺.

Ammonium sulfate fractionation decreased the yield …

An Economic Analysis Of Sprinkling For Bloom Delay And Freeze Protection Of Apples In Farmington, Utah, Jay Val Anderson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The major purpose of this study is to analyze the economic feasibility of bloom delay by sprinkling as a means of protecting delicious apples from frost. The framework of this study is based on decision making theory under uncertainty. It demonstrates the usefulness of the Bayesian approach to determine optimum action to take in face of uncertain climatic conditions. The economic analysis was conducted for Farmington, Utah, where significant relationships were found between the end of winter rest (end of chill-unit accumulation) and time of full bloom of red delicious apples.

A posteriori probabilities for the state of nature were …