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Comparison Of Indigo Carmine Decolorization By Pseudomonas Aeruginosa And Crude Laccase Enzyme From Funalia Trogii, Fi̇li̇z Boran, Emre Bi̇rhanli, Özfer Yeşi̇lada, Eli̇f Özbey
Comparison Of Indigo Carmine Decolorization By Pseudomonas Aeruginosa And Crude Laccase Enzyme From Funalia Trogii, Fi̇li̇z Boran, Emre Bi̇rhanli, Özfer Yeşi̇lada, Eli̇f Özbey
Turkish Journal of Biology
The effects of incubation time, temperature, initial pH, and dye concentration on the indigo carmine decolorization activity of Pseudomonas aeruginosa ATCC 10145 and some factors on the decolorization potential of crude laccase enzyme obtained from Funalia trogii ATCC 200800 were comparatively investigated. This bacterium showed effective decolorization activity at all agitation and temperature values. Indigo carmine was greatly decolorized by P. aeruginosa at all pH values except pH 10. A decrease in decolorization activity occurred with increasing dye concentration, but this bacterium effectively decolorized the dye within 24 h. The decolorization process was through microbial metabolism, not biosorption. No decolorization …
Proteomic And Physiological Responses In Mangrove Kandelia Candel Roots Under Short-Term High-Salinity Stress, Jianhong Xing, Dezhuo Pan, Lingxia Wang, Fanglin Tan, Wei Chen
Proteomic And Physiological Responses In Mangrove Kandelia Candel Roots Under Short-Term High-Salinity Stress, Jianhong Xing, Dezhuo Pan, Lingxia Wang, Fanglin Tan, Wei Chen
Turkish Journal of Biology
Kandelia candel is one of the mangrove species that are most resistant to environmental stress. As a typical nonsalt-secreting mangrove plant, K. candel is an ideal biological material to analyze the molecular mechanism of salt tolerance in woody plants. In this study, changes in protein abundance and expression profile in K. candel roots under high-salinity stress of 600 mmol L-1 NaCl were analyzed using isobaric tags for relative and absolute quantification (iTRAQ) assay. Moreover, the physiological parameters associated with metabolic pathways in which the differentially abundant proteins (DAPs) are involved were determined. A total of 5577 proteins were identified by …
The Nlrp3 Inflammasome: A New Player In Neurological Diseases, Eli̇f Eren, Nesri̇n Özören
The Nlrp3 Inflammasome: A New Player In Neurological Diseases, Eli̇f Eren, Nesri̇n Özören
Turkish Journal of Biology
Inflammasomes are supramolecular protein complexes implicated in the detection of pathogens or danger-associated molecules and are responsible for mounting the first line of innate immune response to counteract these signals and restore tissue homeostasis. Among different inflammasomes identified so far, NLRP3 is of main interest since mutations in Nlrp3 gene are associated with autoinflammatory diseases such as Muckle-Wells syndrome, neonatal onset multisystem inflammatory disease, and familial cold urticaria/autoinflammatory syndrome. On the other hand, whereas other inflammasomes are mainly detectors of specific molecular motifs, NLRP3 is acting as a general sensor of cellular perturbations including potassium efflux, lysosomal damage, and ROS …
Response Of Breast Cancer Cells To Ifnî±-2b In 2d And 3d Cell Cultures, Tetiana Herheliuk, Olena Perepelytsina, Andrij Ugnivenko, Lyudmila Ostapchenko, Mychailo Sydorenko
Response Of Breast Cancer Cells To Ifnî±-2b In 2d And 3d Cell Cultures, Tetiana Herheliuk, Olena Perepelytsina, Andrij Ugnivenko, Lyudmila Ostapchenko, Mychailo Sydorenko
Turkish Journal of Biology
The effect of IFNα-2b on the migration, proliferation, and expression of epithelial and mesenchymal markers of MCF-7 tumor adenocarcinoma cells in 2D and 3D cell cultures was examined. A significant cytostatic effect of IFNα-2b on the tumor population was detected. It was found that changes in the expression of epithelial (CKs and EpCAM) and mesenchymal markers were caused by changing the growth type of the tumor population. IFNα-2b inhibited migration of tumor cells to the suspension fraction and promoted an increase in expression of CK and EpCAM in 2D and 3D cell cultures, but only in the 3D culture was …
Functional Characteristics Of Lactobacilli From Traditional Bulgarian Fermented Milk Products, Veronica Nemska, Petya Logar, Tanya Rasheva, Zdravka Sholeva, Nelly Georgieva, Svetla Danova
Functional Characteristics Of Lactobacilli From Traditional Bulgarian Fermented Milk Products, Veronica Nemska, Petya Logar, Tanya Rasheva, Zdravka Sholeva, Nelly Georgieva, Svetla Danova
Turkish Journal of Biology
After oral administration, probiotic lactobacilli meet a number of protection systems in the human body, such as exposure to gastric, pancreatic, and small intestinal juices. Overcoming these detrimental barriers allows living bacteria to adhere to the intestinal epithelium and permanently colonize the gastrointestinal tract (GIT), providing health benefits to the host. Based on this, the transit tolerance of 25 candidate probiotic lactobacilli from katak, yoghurt, and white-brined and yellow cheese to simulated bile and small intestinal juices of variable pH was investigated. To establish their resistance, in vitro model systems based on modified MRS media and a longer duration of …
Establishment Of Human Trabecular Meshwork Cell Cultures Usingnontransplantable Corneoscleral Rims, Kosala Dinuka Waduthanthri, Carlo Montemagno, Si̇bel Çeti̇nel
Establishment Of Human Trabecular Meshwork Cell Cultures Usingnontransplantable Corneoscleral Rims, Kosala Dinuka Waduthanthri, Carlo Montemagno, Si̇bel Çeti̇nel
Turkish Journal of Biology
Human trabecular meshwork (hTM) cell isolation in academic settings utilizes the motile nature of these cells, allowing them to migrate away from the explant and proliferate on distal regions of the culture substrate. Corneoscleral rims used for transplantation are a potential source of explants for the establishment of hTM cell cultures. However, cell isolation and the initiation of primary cell cultures from ocular tissues stored in Optisol-GS medium for an extended period of time (>6 days) has proven difficult, since Optisol-GS remarkably reduces cell viability and cellularity. Therefore, explants obtained from ocular tissues stored in Optisol-GS do not often …
Identification Of Bioactive Compounds From Fraxinus Angustifolia Extracts With Anti-Nadh Oxidase Activity Of Bovine Milk Xanthine Oxidoreductase, Nadjia Ahmane, Dina Atmani-Kilani, Nassima Chaher, Karima Ayouni, Meriem Rahmani-Berboucha, Gregory Da Costa, Nadjet Debbache-Benaida, Tristan Richard, Djebbar Atmani
Identification Of Bioactive Compounds From Fraxinus Angustifolia Extracts With Anti-Nadh Oxidase Activity Of Bovine Milk Xanthine Oxidoreductase, Nadjia Ahmane, Dina Atmani-Kilani, Nassima Chaher, Karima Ayouni, Meriem Rahmani-Berboucha, Gregory Da Costa, Nadjet Debbache-Benaida, Tristan Richard, Djebbar Atmani
Turkish Journal of Biology
Fraxinus angustifolia leaves and bark are used in traditional medicine against various inflammatory-related pathologies incumbent to reactive oxygen species (ROS) generation by the NADH oxidase activity of enzymes such as xanthine oxidoreductase (XOR). This study was designed to investigate the in vitro and in vivo inhibitory activities of this enzyme by Fraxinus angustifolia extracts. The leaf organic phase of ethyl acetate (LFA) and its bark aqueous counterpart (BFA) showed the strongest anti-NADH oxidase activity in vitro (IC50 = 38.51 and 42.04 μg mL-1, respectively). They consequently suppressed superoxide generation both enzymatically (53% and 19%, respectively) and nonenzymatically (34% and 19%, …
Overexpression Of A Fusion Defensin Gene From Radish And Fenugreek Improves Resistance Against Leaf Spot Diseases Caused By Cercospora Arachidicola And Phaeoisariopsis Personata In Peanut, Madhu Bala, Thankappan Radhakrishnan, Abhay Kumar, Gyan Prakash Mishra, Jentilal Ramjibhai Dobraia, Pulugurtha Bharadwaja Kirti
Overexpression Of A Fusion Defensin Gene From Radish And Fenugreek Improves Resistance Against Leaf Spot Diseases Caused By Cercospora Arachidicola And Phaeoisariopsis Personata In Peanut, Madhu Bala, Thankappan Radhakrishnan, Abhay Kumar, Gyan Prakash Mishra, Jentilal Ramjibhai Dobraia, Pulugurtha Bharadwaja Kirti
Turkish Journal of Biology
No abstract provided.
Characterization Of Mesenchymal Stem Cells In Mucolipidosis Type Ii (I-Cell Disease), Sevi̇l Köse, Fati̇ma Aerts Kaya, Bülent Bariş Kuşkonmaz, Fahri̇ye Duygu Çeti̇nkaya
Characterization Of Mesenchymal Stem Cells In Mucolipidosis Type Ii (I-Cell Disease), Sevi̇l Köse, Fati̇ma Aerts Kaya, Bülent Bariş Kuşkonmaz, Fahri̇ye Duygu Çeti̇nkaya
Turkish Journal of Biology
Mucolipidosis type II (ML-II, I-cell disease) is a fatal inherited lysosomal storage disease caused by a deficiency of the enzyme N-acetylglucosamine-1-phosphotransferase. A characteristic skeletal phenotype is one of the many clinical manifestations of ML-II. Since the mechanisms underlying these skeletal defects in ML-II are not completely understood, we hypothesized that a defect in osteogenic differentiation of ML-II bone marrow mesenchymal stem cells (BM-MSCs) might be responsible for this skeletal phenotype. Here, we assessed and characterized the cellular phenotype of BM-MSCs from a ML-II patient before (BBMT) and after BM transplantation (ABMT), and we compared the results with BM-MSCs from a …
Squalene Attenuates The Oxidative Stress And Activates Akt/Mtor Pathway Against Cisplatin-Induced Kidney Damage In Mice, Ayşe Arzu Şakul, Mehmet Ozansoy, Bi̇rsen Eli̇bol, Şule Ayla, Mehmet Yalçin Günal, Yasemi̇n Yozgat, Selma Hüveyda Başağa, Kazi̇m Şahi̇n, Rümeyza Kazancioğlu, Ülkan Kiliç
Squalene Attenuates The Oxidative Stress And Activates Akt/Mtor Pathway Against Cisplatin-Induced Kidney Damage In Mice, Ayşe Arzu Şakul, Mehmet Ozansoy, Bi̇rsen Eli̇bol, Şule Ayla, Mehmet Yalçin Günal, Yasemi̇n Yozgat, Selma Hüveyda Başağa, Kazi̇m Şahi̇n, Rümeyza Kazancioğlu, Ülkan Kiliç
Turkish Journal of Biology
The clinical use of cisplatin, which is a first-line anticancer agent, is highly restricted due to its adverse effects on kidneys that lead to nephrotoxicity. Therefore, some potential reno-protective substances have been used in combination with cisplatin to cope with nephrotoxicity. Due to its high antitumor activity and oxygen-carrying capacity, we investigated the molecular effects of squalene against cisplatin-induced oxidative stress and kidney damage in mice. Single dose of cisplatin (7 mg/kg) was given to male Balb/c mice. Squalene (100 mg/kg/day) was administered orogastrically to mice for 10 days. Following sacrification, molecular alterations were investigated as analysis of the levels …
Characterizing Microsatellite Polymorphisms Using Assembly-Based Andmapping-Based Tools, Gülfem Demi̇r, Can Alkan
Characterizing Microsatellite Polymorphisms Using Assembly-Based Andmapping-Based Tools, Gülfem Demi̇r, Can Alkan
Turkish Journal of Biology
Microsatellite polymorphism has always been a challenge for genome assembly and sequence alignment due to sequencing errors, short read lengths, and high incidence of polymerase slippage in microsatellite regions. Despite the information they carry being very valuable, microsatellite variations have not gained enough attention to be a routine step in genome sequence analysis pipelines. After the completion of the 1000 Genomes Project, which aimed to establish the most detailed genetic variation catalog for humans, the consortium released only two microsatellite prediction sets generated by two tools. Many other large research efforts have failed to shed light on microsatellite variations. We …
E2f6 Is Essential For Cell Viability In Breast Cancer Cells During Replication Stress, Inam Lafta
E2f6 Is Essential For Cell Viability In Breast Cancer Cells During Replication Stress, Inam Lafta
Turkish Journal of Biology
E2F6 is a member of the E2F family of transcription factors involved in regulation of a wide variety of genes through both activation and repression. E2F6 has been reported as overexpressed in breast cancers but whether or not this is important for tumor development is unclear. We first checked E2F6 expression in tumor cDNAs and the protein level in a range of breast cancer cell lines. RNA interference-mediated depletion was then used to assess the importance of E2F6 expression in cell lines with regard to cell cycle profile using fluorescence-activated cell sorting and a cell survival assay using (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide …
Prenatal Exposure Of Diclofenac Sodium Alters The Behavioral Development Of Young Wistar Rats, Bi̇rsen Eli̇bol, Begüm Oğur, Havva Doğru
Prenatal Exposure Of Diclofenac Sodium Alters The Behavioral Development Of Young Wistar Rats, Bi̇rsen Eli̇bol, Begüm Oğur, Havva Doğru
Turkish Journal of Biology
Diclofenac sodium (DS), a potent inhibitor of cyclooxygenase, reduces the release of arachidonic acid and formation of prostaglandins. Being a nonsteroid drug that shows antiinflammatory action, the possible side effects of fetal DS administration gain importance in public and medical applications. Herein, the effects of DS administration (1 mg/kg) during gestational days 5-20 were investigated on the performance of Wistar rat pups in a variety of behavioral tasks. Four-week-old pups were subjected to sensory motor tests, a plus maze, an open field, the Morris water maze, and a radial arm maze. Fetal DS disrupted some sensory motor performances, such as …
In Vitro Study On Chondrogenic Differentiation Of Human Adipose-Derived Stem Cells On Treated Bovine Pericardium, My Thi Ngoc Nguyen, Vu Nguyen Doan, Ha Le Bao Tran
In Vitro Study On Chondrogenic Differentiation Of Human Adipose-Derived Stem Cells On Treated Bovine Pericardium, My Thi Ngoc Nguyen, Vu Nguyen Doan, Ha Le Bao Tran
Turkish Journal of Biology
Bovine pericardium has been proposed as an available material for tissue engineering and bioprosthetic reconstruction. In this study, bovine pericardium was fabricated into a scaffold for culturing and chondrogenic differentiation of human adipose-derived stem cells (hADSCs). Bovine pericardium was treated in 10 mM Tris-HCl and 0.15% SDS, followed by crosslinking in 0.1% glutaraldehyde. Treated bovine pericardium (tBP) was characterized as a slight yellowish thin membrane with enhanced tensile strength and strain property. The membrane maintained stability under enzymatic conditions for up to 16 days of incubation. The results confirmed tBP as a cell-friendly scaffold for hADSCs due to low cytotoxicity …
Development Of Antimicrobial Gelatin Films With Boron Derivatives, Sanem Argin, Merve Güleri̇m, Fi̇kretti̇n Şahi̇n
Development Of Antimicrobial Gelatin Films With Boron Derivatives, Sanem Argin, Merve Güleri̇m, Fi̇kretti̇n Şahi̇n
Turkish Journal of Biology
Food packaging technology has been advancing to provide safe and high quality food products and to minimize food waste. Moreover, there is a dire need to replace plastic materials in order to reduce environmental pollution. The aim of this study was to prepare biodegradable antimicrobial packaging films from gelatin. Boric acid, disodium octaborate tetrahydrate, and sodium pentaborate were incorporated as the antimicrobial agents. Films containing boric acid and its salts showed antibacterial effect against Staphylococcus aureus and Pseudomonas aeruginosa, as well as antifungal and anticandidal effects against Aspergillus niger and Candida albicans. The mechanical strength of the films was mostly …
Effects Of Notch Signalling On The Expression Of Sema3c, Hmga2, Cxcl14, Cxcr7, And Ccl20 In Breast Cancer, Cansu Küçükköse, Özden Yalçin Özuysal
Effects Of Notch Signalling On The Expression Of Sema3c, Hmga2, Cxcl14, Cxcr7, And Ccl20 In Breast Cancer, Cansu Küçükköse, Özden Yalçin Özuysal
Turkish Journal of Biology
Metastasis is the main reason for death in breast cancer. Understanding the molecular players in metastasis is crucial for diagnostic and therapeutic purposes. Notch signalling plays an oncogenic role in breast tumorigenesis and is involved in metastasis. Downstream mediators of Notch signalling in prometastatic processes are not yet fully discovered. Here we aimed to investigate whether Notch signalling regulates the expression of SEMA3C, HMGA2, CXCL14, CXCR7, and CCL20, which are involved in prometastatic processes, in breast cell lines. To this end, expression of the selected genes was analysed following Notch activation by overexpression of the Notch1 intracellular domain in the …
A Magnetic Affinity Approach To Identify Plant Gaba-Binding Proteins, Jie Zou, Jingzhe Guo, Shisheng Li
A Magnetic Affinity Approach To Identify Plant Gaba-Binding Proteins, Jie Zou, Jingzhe Guo, Shisheng Li
Turkish Journal of Biology
In plants, GABA plays a critical role in sexual plant reproduction; however, GABA receptors and the associated detailed signaling mechanisms remain to be elucidated. Our experiments show that the proposed technique is reliable and convenient for probing GABA-binding proteins and could be applicable in similar projects by covalently immobilizing the free carboxylic group of GABA on magnetic beads (SiMAG-Carboxyl). New probes produced by covalently immobilizing the free carboxylic group of GABA on magnetic beads (SiMAG-Carboxyl) can obtain useful information on GABA receptors in plants.
A Novel 1,4-Naphthoquinone-Derived Compound Induces Apoptotic Cell Deathin Breast Cancer Cells, Di̇dem Karakaş Zeybek, Remzi̇ Okan Akar, Zeli̇ha Gökmen, Nahi̇de Gülşah Deni̇z, Engi̇n Ulukaya
A Novel 1,4-Naphthoquinone-Derived Compound Induces Apoptotic Cell Deathin Breast Cancer Cells, Di̇dem Karakaş Zeybek, Remzi̇ Okan Akar, Zeli̇ha Gökmen, Nahi̇de Gülşah Deni̇z, Engi̇n Ulukaya
Turkish Journal of Biology
Breast cancer is the most-diagnosed cancer type among women. The triple-negative subtype is an especially aggressive type of breast cancer. Although chemotherapy is almost the only option for the treatment of triple-negative breast cancer (TNBC), currently used chemotherapeutics are not effective enough, considering the poor survival rate of patients. Therefore, novel compounds need to be developed to improve survival rates. It has been known that quinonic compounds, which are found in nature, have antibacterial, antifungal, and antitumorigenic properties. Naphthoquinones are members of the quinone family and are widely used in research due to their promising properties. In this study, we …
Knockdown Of Set Domain, Bifurcated 1 Suppresses Head And Neck Cancer Cell Viability And Wound-Healing Ability In Vitro, Si̇bel Özdaş
Knockdown Of Set Domain, Bifurcated 1 Suppresses Head And Neck Cancer Cell Viability And Wound-Healing Ability In Vitro, Si̇bel Özdaş
Turkish Journal of Biology
Head and neck cancer (HNC) is the sixth most common cancer worldwide and therefore presents a global public health problem. There are no standard algorithms for the diagnosis and follow-up of the disease, and no effective current treatment approaches exist. Therefore, the discovery of new biomolecules and the design of new strategies to aid in early diagnosis is necessary, along with establishing prognostic factors of HNC. In several cancer studies, the upregulation of SET Domain, Bifurcated 1 (SETDB1) has been reported to be tumor-inducing and to indicate a cancer-invasive prognosis, leading to the modulation of genes associated with different signaling …
A New Helicoverpa Armigera Nucleopolyhedrovirus Isolate From Heliothis Peltigera (Denis & Schiffermuller) (Lepidoptera: Noctuidae) In Turkey, Gözde Büşra Eroğlu, Remzi̇ye Nalçacioğlu, Zi̇hni̇ Demi̇rbağ
A New Helicoverpa Armigera Nucleopolyhedrovirus Isolate From Heliothis Peltigera (Denis & Schiffermuller) (Lepidoptera: Noctuidae) In Turkey, Gözde Büşra Eroğlu, Remzi̇ye Nalçacioğlu, Zi̇hni̇ Demi̇rbağ
Turkish Journal of Biology
This study reports a new Helicoverpa armigera nucleopolyhedrovirus (NPV) isolated from Heliothis peltigera (Denis & Schiffermuller), collected in the vicinity of Adana, Turkey. Infection was confirmed by tissue polymerase chain reaction and sequence analysis. Results showed that dead H. peltigera larvae contain Helicoverpa armigera nucleopolyhedrovirus. Thus, the isolate was named as HearNPV-TR. Microscopy studies indicated that occlusion bodies were 0.73 to 1.66 μm in diameter. The nucleocapsids are approximately 184 × 41 nm in size. The genome of HearNPV-TR was digested with KpnI and XhoI enzymes and calculated as 130.5 kb. Phylogenetic analysis showed that HearNPV-TR has close relation with …
Crosstalk Between Autophagy And Apoptosis Induced By Camphor Inschizosaccharomyces Pombe, Hizlan Hincal Ağuş, Sedanur Yilmaz, Cansin Ogeday Şengöz
Crosstalk Between Autophagy And Apoptosis Induced By Camphor Inschizosaccharomyces Pombe, Hizlan Hincal Ağuş, Sedanur Yilmaz, Cansin Ogeday Şengöz
Turkish Journal of Biology
Camphor is widely used in pharmacy, the food industry, and cosmetics. In this study, we evaluate inhibitory and cytotoxic effects of camphor in the fission yeast (Schizosaccharomyces pombe), which presents a unicellular model in mechanistic toxicology and cell biology. Low-dose camphor exposure (0.4 mg/mL) activated autophagy, which was shown by GFP-Atg8 dots and transcriptional upregulation of Atg6 (Beclin-1 ortholog). Autophagy was also confirmed by using autophagy-deficient cells, which showed reduction in GFP-Atg8 dot formation. However, high-dose camphor exposure (0.8 mg/mL) caused dramatic cell death ratios, demonstrated by spot and colony-forming assays, even in autophagy-deficient cells. To unravel the underlying mechanism, …
Identification Of Watermelon Heat Shock Protein Members And Tissue-Specific Gene Expression Analysis Under Combined Drought And Heat Stresses, Yasemi̇n Çeli̇k Altunoğlu, Merve Keleş, Tevfi̇k Hasan Can, Mehmet Cengi̇z Baloğlu
Identification Of Watermelon Heat Shock Protein Members And Tissue-Specific Gene Expression Analysis Under Combined Drought And Heat Stresses, Yasemi̇n Çeli̇k Altunoğlu, Merve Keleş, Tevfi̇k Hasan Can, Mehmet Cengi̇z Baloğlu
Turkish Journal of Biology
Heat shock protein (Hsp) gene family members in the watermelon genome were identified and characterized by bioinformatics analysis. In addition, expression profiles of genes under combined drought and heat stress conditions were experimentally analyzed. In the watermelon genome, 39 genes belonging to the sHsp family, 101 genes belonging to the Hsp40 family, 23 genes belonging to the Hsp60 family, 12 genes belonging to the Hsp70 family, 6 genes belonging to the Hsp90 family, and 102 genes belonging to the Hsp100 family were found. It was also observed that the proteins in the same cluster in the phylogenetic trees had similar …
Effects Of B2o3 (Boron Trioxide) On Colon Cancer Cells: Our First-Step Experience And In Vitro Results, Özgür Albuz, Di̇lek Dülger, Beste Çağdaş Tunali, Feray Aydin, Seli̇m Yalçin, Mustafa Türk
Effects Of B2o3 (Boron Trioxide) On Colon Cancer Cells: Our First-Step Experience And In Vitro Results, Özgür Albuz, Di̇lek Dülger, Beste Çağdaş Tunali, Feray Aydin, Seli̇m Yalçin, Mustafa Türk
Turkish Journal of Biology
Boron oxide (B2O3) is derived from dehydration of boric acid and is a colorless, semitransparent, crystalline compound that is moderately soluble in water. On the other hand, boron oxide is chemically hygroscopic. This gives the molecule the ability to soak up water and adhere to tissues. Boron oxide can be used locally after tumor debulking in inoperable tumors and especially when the tumor-free margin distance cannot be provided. For all these reasons we aimed to evaluate the in vitro test results of B2O3 in terms of cytotoxicity, genotoxicity, apoptosis, and necrotic effects on L929 fibroblast cells and DLD-1 colorectal adenocarcinoma …
Prediction And Expression Analysis Of G Protein-Coupled Receptors In The Laboratory Stick Insect, Carausius Morosus, Burçi̇n Duan Şahbaz, Necla Bi̇rgül İyi̇son
Prediction And Expression Analysis Of G Protein-Coupled Receptors In The Laboratory Stick Insect, Carausius Morosus, Burçi̇n Duan Şahbaz, Necla Bi̇rgül İyi̇son
Turkish Journal of Biology
G protein-coupled receptors (GPCRs) are 7-transmembrane proteins that transduce various extracellular signals into intracellular pathways. They are the major target of neuropeptides, which regulate the development, feeding behavior, mating behavior, circadian rhythm, and many other physiological functions of insects. In the present study, we performed RNA sequencing and de novo transcriptome assembly to uncover the GPCRs expressed in the stick insect Carausius morosus. The transcript assemblies were predicted for the presence of 7-transmembrane GPCR domains. As a result, 430 putative GPCR transcripts were obtained and 43 of these revealed full-length sequences with highly significant similarity to known GPCR sequences in …
Fibrous Bone Tissue Engineering Scaffolds Prepared By Wet Spinning Of Plga, Nergi̇s Abay Akar, Görke Gürel Peközer, Gamze Köse
Fibrous Bone Tissue Engineering Scaffolds Prepared By Wet Spinning Of Plga, Nergi̇s Abay Akar, Görke Gürel Peközer, Gamze Köse
Turkish Journal of Biology
Having a self-healing capacity, bone is very well known to regenerate itself without leaving a scar. However, critical size defects due to trauma, tumor, disease, or infection involve bone graft surgeries in which complication rate is relatively at high levels. Bone tissue engineering appears as an alternative for grafting. Fibrous scaffolds are useful in tissue engineering applications since they have a high surface-to-volume ratio, and adjustable, highly interconnected porosity to enhance cell adhesion, survival, migration, and proliferation. They can be produced in a wide variety of fiber sizes and organizations. Wet spinning is a convenient way to produce fibrous scaffolds …
Microrna Prediction Based On 3d Graphical Representation Of Rnasecondary Structures, Müşerref Duygu Saçar Demi̇rci̇
Microrna Prediction Based On 3d Graphical Representation Of Rnasecondary Structures, Müşerref Duygu Saçar Demi̇rci̇
Turkish Journal of Biology
MicroRNAs (miRNAs) are posttranscriptional regulators of gene expression. While a miRNA can target hundreds of messenger RNA (mRNAs), an mRNA can be targeted by different miRNAs, not to mention that a single miRNA might have various binding sites in an mRNA sequence. Therefore, it is quite involved to investigate miRNAs experimentally. Thus, machine learning (ML) is frequently used to overcome such challenges. The key parts of a ML analysis largely depend on the quality of input data and the capacity of the features describing the data. Previously, more than 1000 features were suggested for miRNAs. Here, it is shown that …
Morphophysiological And Biochemical Response Of Mungbean [Vigna Radiata (L.) Wilczek] Varieties At Different Developmental Stages Under Drought Stress, Pooja Bangar, Ashok Chaudhury, Bhavana Tiwari, Sanjay Kumar, Ratna Kumari, Kangila Venkataramana Bhat
Morphophysiological And Biochemical Response Of Mungbean [Vigna Radiata (L.) Wilczek] Varieties At Different Developmental Stages Under Drought Stress, Pooja Bangar, Ashok Chaudhury, Bhavana Tiwari, Sanjay Kumar, Ratna Kumari, Kangila Venkataramana Bhat
Turkish Journal of Biology
The present study was conducted to assess the morphophysiological and biochemical responses during different developmental stages in mungbean varieties subjected to drought stress, and to screen the varieties for drought tolerance. A field experiment was performed according to a completely randomized design on 25 mungbean varieties with 3 replicates per variety. Stress treatment was applied at 3 levels: control (no stress), vegetative stage (25 days after sowing), and reproductive stage (35 days after sowing). According to combined analysis of variance, there were significant effects from drought stress on relative water content (RWC), membrane stability index (MSI), protein and proline content …
Systematic Analysis Of The Frequently Amplified 2p15-P16.1 Locus Reveals Papolg As A Potential Proto-Oncogene In Follicular And Transformed Follicular Lymphoma, Deni̇z Kurşun, Can Küçük
Systematic Analysis Of The Frequently Amplified 2p15-P16.1 Locus Reveals Papolg As A Potential Proto-Oncogene In Follicular And Transformed Follicular Lymphoma, Deni̇z Kurşun, Can Küçük
Turkish Journal of Biology
Transformed follicular lymphoma (tFL) originates from histological transformation of follicular lymphoma (FL), which is the most common indolent non-Hodgkin lymphoma. High-resolution genomic copy-number analysis previously identified frequent amplification of the 2p15-p16.1 locus in FL and tFL cases. The genes (i.e. BCL11A, PAPOLG, PUS10, and USP34) in this amplified locus have not been systematically investigated to date in terms of their role in FL pathogenesis or transformation to tFL. Here we investigated the relationship between amplification and expression of genes in 2p15-p16.1 as well as their expression after histological transformation. NCBI GEO SNP array and gene expression profile (GEP) data of …
Key Actors In Cancer Therapy: Epigenetic Modifiers, Remzi̇ Okan Akar, Seli̇n Selvi̇, Engi̇n Ulukaya, Nazlihan Aztopal
Key Actors In Cancer Therapy: Epigenetic Modifiers, Remzi̇ Okan Akar, Seli̇n Selvi̇, Engi̇n Ulukaya, Nazlihan Aztopal
Turkish Journal of Biology
Epigenetic reprogramming plays a crucial role in the tumorigenicity and maintenance of tumor-specific gene expression that especially occurs through DNA methylation and/or histone modifications. It has well-defined mechanisms. It is known that alterations in the DNA methylation pattern and/or the loss of specific histone acetylation/methylation markers are related to several hallmarks of cancer, such as drug resistance, stemness, epithelial-mesenchymal transition, and metastasis. It has also recently been highlighted that epigenetic alterations are critical for the regulation of the stemlike properties of cancer cells (tumor-initiating cells; cancer stem cells). Cancer stem cells are thought to be responsible for the recurrence of …
Contribution Of Hepatitis B Virus X Protein-Induced Aberrant Microrna Expression To Hepatocellular Carcinoma Pathogenesis, Zhiyuan Wei, Xiaohe Shen, Bing Ni, Gaoxing Luo, Yi Tian, Yi Sun
Contribution Of Hepatitis B Virus X Protein-Induced Aberrant Microrna Expression To Hepatocellular Carcinoma Pathogenesis, Zhiyuan Wei, Xiaohe Shen, Bing Ni, Gaoxing Luo, Yi Tian, Yi Sun
Turkish Journal of Biology
The hepatitis B virus-encoded X (HBX) protein plays important roles in Hepatocellular carcinoma (HCC). Previous studies have demonstrated that HBX can induce alterations in the expression of numerous microRNAs (miRNAs) involved in the carcinogenesis of various tumors. However, the global profile of liver miRNA changes induced by HBX has not been characterized. In this study, we conducted a miRNA microarray analysis to investigate the influence of HBX on the expression of total miRNAs in liver in relation to HCC. Comparative analysis of the data from human normal liver cells (L02) and human HCC cells (HepG2), with or without HBX, identified …