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Differentiation Of Three Phenotypically Similar Blattella Spp.: Analysis With Polymerase Chain Reaction-Restriction Fragment Length Polymorphism Of Mitochondrial Dna, Pari Pachamuthu, Shripat T. Kamble, Thomas L. Clark, John E. Foster
Differentiation Of Three Phenotypically Similar Blattella Spp.: Analysis With Polymerase Chain Reaction-Restriction Fragment Length Polymorphism Of Mitochondrial Dna, Pari Pachamuthu, Shripat T. Kamble, Thomas L. Clark, John E. Foster
John E. Foster
Differentiation of Blattella asahinai Mizukubo, Blattella vaga Hebard, and Blattella germanica (L.) was investigated using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). DNA fragments corresponding to mitochondrial CO1,CO1/TL2(small and large fragments), and ITS2 regions were amplified using PCR. The universal primers used for amplifying mitochondrial CO1 and CO1/TL2 (small and large fragments) were CO1-J-1718/CO1- N-2191, CO1-J-2441/TL2-N-3014, and CO1-J-1751/TL2-N-3014. Amplification was observed with all primer combinations, but diagnostic patterns were found only with AluI digested amplicons of CO1 and CO1/TL2 (large fragments) regions. Both inter/intraspecific polymorphism was observed for the 517 bp CO1 region between German (A, B) and Asian …