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Choosing A Bt Transgenic Corn Hybrid, R. J. Wright, Thomas E. Hunt, J. F. Witkowski, Blair Siegfried, John E. Foster Dec 2015

Choosing A Bt Transgenic Corn Hybrid, R. J. Wright, Thomas E. Hunt, J. F. Witkowski, Blair Siegfried, John E. Foster

John E. Foster

Describes traits of various Bt corn hybrids and factors to consider when selecting and using these hybrids.


Genetic Variation And Inheritance Of Diapause Induction In Two Distinct Voltine Ecotypes Of Ostrinia Nubilalis (Lepidoptera: Crambidae), Cengis Ikten, Steven R. Skoda, Thomas E. Hunt, Jaime Molina-Ochoa, John E. Foster Mar 2012

Genetic Variation And Inheritance Of Diapause Induction In Two Distinct Voltine Ecotypes Of Ostrinia Nubilalis (Lepidoptera: Crambidae), Cengis Ikten, Steven R. Skoda, Thomas E. Hunt, Jaime Molina-Ochoa, John E. Foster

John E. Foster

European corn borer, Ostrinia nubilalis (Hübner) (Lepidoptera: Crambidae), displays a larval diapause in response to short photoperiods and is adapted to a variety of local conditions throughout North America. Hence, the effective photoperiod inducing larval diapause will differ among geographic ecotypes. This study considers the inheritance of photoperiodic larval diapause induction by hybridization and backcrossing two latitudinally distinct ecotypes of the European corn borer collected between 41° N, 96° W and 48° N, 96° W and under a range of photoperiods representative of their respective locations: from 14:10 to 16:8 (L:D) h. The ecotype adapted to a bivoltine habitat (southeastern …


Cross-Resistance And Resistance Longevity As Induced By Bean Leaf Beetle, Cerotoma Trifurcata And Soybean Looper, Pseudoplusia Includens Herbivory On Soybean, P. Srinivas, Stephen D. Danielson, C. Michael Smith, John E. Foster Mar 2012

Cross-Resistance And Resistance Longevity As Induced By Bean Leaf Beetle, Cerotoma Trifurcata And Soybean Looper, Pseudoplusia Includens Herbivory On Soybean, P. Srinivas, Stephen D. Danielson, C. Michael Smith, John E. Foster

John E. Foster

Cross-resistance, and longevity of resistance, induced by the bean leaf beetle, Cerotoma trifurcata, was studied IN the soybean PI 227687 that exhibited induced response in earlier studies. Bean leaf beetle adults and soybean looper, Pseudoplusia includens, larvae were used to induce resistance and to determine beetle feeding preference. Beetles were collected from soybean fields 2 to 5 days prior to the feeding preference test. The level of cross-resistance induced by soybean looper herbivory to subsequent bean leaf beetle feeding was higher when compared to cross-resistance induced by bean leaf beetle herbivory against subsequent feeding by soybean looper. Further, herbivory by …


Genetic Variability Of The European Corn Borer, Ostrinia Nubilalis, Suggests Gene Flow Between Populations In The Midwestern United States, Jeffrey T. Krumm, Thomas E. Hunt, Steven R. Skoda, Gary L. Hein, Donald J. Lee, Pete L. Clark, John E. Foster Mar 2012

Genetic Variability Of The European Corn Borer, Ostrinia Nubilalis, Suggests Gene Flow Between Populations In The Midwestern United States, Jeffrey T. Krumm, Thomas E. Hunt, Steven R. Skoda, Gary L. Hein, Donald J. Lee, Pete L. Clark, John E. Foster

John E. Foster

The European corn borer, Ostrinia nubilalis (Hübner) (Lepidoptera: Crambidae), is a widely distributed and serious economic pest to corn production in the U.S. Genetic variability of O. nubilalis was studied in 18 sub-populations in the upper Midwestern United States using amplified fragment length polymorphism. The relatively low GST values indicate that more variation exists within populations than between populations. High gene flow (Nm) values were indicated across the entire O. nubilalis population; the lowest degree of gene flow was in the northern samples (Nm = 1.96) and the highest degree of gene flow was in the southern samples (Nm = …


Morphological And Genetic Analyses In The Melanoplus Packardii Group (Orthoptera: Acrididae), Mathew L. Brust, Erica J. Lindroth, Wyatt Hoback, Robert Wright, Kathryn J. Hanford, John E. Foster Mar 2012

Morphological And Genetic Analyses In The Melanoplus Packardii Group (Orthoptera: Acrididae), Mathew L. Brust, Erica J. Lindroth, Wyatt Hoback, Robert Wright, Kathryn J. Hanford, John E. Foster

John E. Foster

Melanoplus packardii Scudder was described in 1897. Three additional closely-related species were later described and their status as species has been questioned on numerous occasions. We examined morphology from specimens collected in Nebraska which fit descriptions of three of the four forms and specimens that appeared to be hybrids. We found distinct morphological characters suggesting species status for M. foedus and M. packardii, but not for M. foedus fluviatilis. Examination of aedeagi of these three forms suggests that M. foedus and M. packardii are each distinct, but that the aedeagi of M. f. fluviatilis and M. f. foedus cannot be …


Evaluation Of Buffalograss Germplasm For Resistance To Blissus Occiduus (Hemiptera: Lygaeidae), Tiffany Heng-Moss, Frederick P. Baxendale, Terrance P. Riordan, John E. Foster Mar 2012

Evaluation Of Buffalograss Germplasm For Resistance To Blissus Occiduus (Hemiptera: Lygaeidae), Tiffany Heng-Moss, Frederick P. Baxendale, Terrance P. Riordan, John E. Foster

John E. Foster

Blissus occiduus Barber has emerged as an important insect pest of buffalograss, Buchloe dactyloides (Nuttall) Engelmann, in Nebraska. This research evaluated selected buffalograss germplasm for resistance to B. occiduus. Eleven buffalograss selections were screened for chinch bug resistance in three greenhouse studies and two field evaluations. Based on chinch bug damage, NE91-118, ‘Tatanka’, ‘Bonnie Brae’, and ‘Cody’ were rated highly to moderately resistant. These four buffalograsses exhibited minimal damage, even though all were heavily infested with chinch bugs. NE84-45-3 and ‘378’ were highly susceptible to B. occiduus. Field evaluations confirmed chinch bug resistance ratings under field conditions. NE91-118 displayed high …


Lyophilization Of Spodoptera Frugiperda (Lepidoptera: Noctuidae) Larvae Yields High-Quality Dna For Use In Aflp Genetic Studies, Pete L. Clark, David J. Isenhour, Steven R. Skoda, Jaime Molina-Ochoa, Claudia Gianni, John E. Foster Mar 2012

Lyophilization Of Spodoptera Frugiperda (Lepidoptera: Noctuidae) Larvae Yields High-Quality Dna For Use In Aflp Genetic Studies, Pete L. Clark, David J. Isenhour, Steven R. Skoda, Jaime Molina-Ochoa, Claudia Gianni, John E. Foster

John E. Foster

Agricultural research in the 21st century has become a collaborative effort. Research on crop pests like Spodoptera frugiperda (J.E. Smith), commonly known as the fall armyworm (FAW), can involve international collaboration because it is a pest not only in the southern United States, but also in Latin and South America. Our interest to study the genetic variation of 24 subpopulations of FAW from the southern United States, Mexico, Puerto Rico, Brazil and Argentina required insect collection procedures that preserve the integrity of DNA for molecular genetic analysis. The samples were collected primarily from maize (Zea mays L.), but also included …


Identification Of Screwworms, Cochliomyia Hominivorax (Coquerel) (Diptera: Calliphoridae), With A Monoclonal Antibody-Based Enzyme-Linked Immunosorbent Assay (Mab-Elisa), J. L. Figarola, Steven R. Skoda, Dennis R. Berkebile, John E. Foster Mar 2012

Identification Of Screwworms, Cochliomyia Hominivorax (Coquerel) (Diptera: Calliphoridae), With A Monoclonal Antibody-Based Enzyme-Linked Immunosorbent Assay (Mab-Elisa), J. L. Figarola, Steven R. Skoda, Dennis R. Berkebile, John E. Foster

John E. Foster

Myiasis caused by screwworms, Cochliomyia hominivorax (Coquerel), is devastating to warmblooded animals and economically important to livestock producers. It is difficult to distinguish these pests, immature screwworms, from immatures of other non-pest fly species that often occur in animal wounds; it would be helpful to have tools available that do not rely on morphological characteristics. We developed twomonoclonal antibodies (MAbs), highly specific for the screwworm, and used them in an enzyme-linked immunosorbent assay (MAb-ELISA), that differentiated screwworm eggs, larvae, pupae, and adults from those of the closely related secondary screwworm, C. macellaria (Fabricius) as well as Phormia regina (Meigen), Phaenicia …


Susceptibility Of Biological Stages Of The Horn Fly, Haematobia Irritans, To Entomopathogenic Fungi (Hyphomycetes), C. A. Angel-Sahagún, R. Lezama-Gutiérrez, J. Molina-Ochoa, E. Galindo-Velasco, M. López-Edwards, O. Rebolledo-Domínguez, C. Cruz-Vázquez, W. P. Reyes-Velázquez, S. R. Skoda, John E. Foster Mar 2012

Susceptibility Of Biological Stages Of The Horn Fly, Haematobia Irritans, To Entomopathogenic Fungi (Hyphomycetes), C. A. Angel-Sahagún, R. Lezama-Gutiérrez, J. Molina-Ochoa, E. Galindo-Velasco, M. López-Edwards, O. Rebolledo-Domínguez, C. Cruz-Vázquez, W. P. Reyes-Velázquez, S. R. Skoda, John E. Foster

John E. Foster

The susceptibility of the egg, pupa, and adult of Haematobia irritans (L.) (Diptera: Muscidae) to isolates of the fungi Metarhizium anisopliae (Metsch.) Sor., Beauveria bassiana (Bals.) Vuill., and Paecilomyces fumosoroseus (Wize) Brown and Smith, was evaluated under laboratory conditions. Groups of 20 eggs than 4 h old, pupae less than 48h old and adults were sprayed with a conidial suspension of each isolate. Eggs, pupae and adults of horn fly were susceptible to these entomopathogenic fungi. For treated eggs, the isolates Ma3, Ma 15, Ma25, Pfr1, and Pfr8 reduced adult emergence to 3.8% to 6.3% in comparison with the control …


Diabrotica Virgifera Virgifera (Coleoptera: Chrysomelidae) Larval Feeding Behavior On Transgenic Maize (Mon 863) And Its Isoline, Pete L. Clark, Ty T. Vaughn, Lance J. Meinke, Jaime Molina-Ochoa, John E. Foster Mar 2012

Diabrotica Virgifera Virgifera (Coleoptera: Chrysomelidae) Larval Feeding Behavior On Transgenic Maize (Mon 863) And Its Isoline, Pete L. Clark, Ty T. Vaughn, Lance J. Meinke, Jaime Molina-Ochoa, John E. Foster

John E. Foster

Diabrotica species (Coleoptera: Chrysomelidae) larval behavior studies have posed a challenge to researchers because of the subterranean life cycle of this pest. To fully understand how the western corn rootworm, Diabrotica virgifera virgifera LeConte, injures the maize, Zea mays L., root system, its behavior must be studied. For example, larvae that can detect an area of the root that has a lower amount of toxin, whether from an insecticide or a transgenic maize plant, have an increased chance of survival. This study assessed D. v. virgifera larval feeding behavior on rootworm-susceptible maize and maize containing a biotechnology-derived trait (MON863) with …


Genetic Structure And Molecular Variability Of Spodoptera Frugiperda (Lepidoptera: Noctuidae) Collected In Maize And Cotton Fields In Brazil, S. Martinelli, P. L. Clark, M. I. Zucchi, M. C. Silva-Filho, John E. Foster, C. Omoto Mar 2012

Genetic Structure And Molecular Variability Of Spodoptera Frugiperda (Lepidoptera: Noctuidae) Collected In Maize And Cotton Fields In Brazil, S. Martinelli, P. L. Clark, M. I. Zucchi, M. C. Silva-Filho, John E. Foster, C. Omoto

John E. Foster

The purpose of this research was to evaluate the genetic similarity and structure of the fall armyworm, Spodoptera frugiperda (J.E. Smith), populations associated with maize and cotton crops in Brazil using amplified fragment length polymorphisms. Mean genetic similarity among populations was 0.45. The unweighted pair group method with arithmetic mean analysis dendrograms did not separate populations of S. frugiperda into clusters related to the host plant in which the insects were collected. No genetic variation was observed among maize and cotton populations of S. frugiperda, suggesting that the same populations are injuring both crops in Brazil. This research validates the …


Hydrolase And Oxido-Reductase Activities In Diuraphis Noxia And Rhopalosiphum Padi (Hemiptera: Aphididae), Xinzhi Ni, Sharron S. Quisenberry, Saowaluck Pornkulwat, James Lester Figarola, Steven R. Skoda, John E. Foster Mar 2012

Hydrolase And Oxido-Reductase Activities In Diuraphis Noxia And Rhopalosiphum Padi (Hemiptera: Aphididae), Xinzhi Ni, Sharron S. Quisenberry, Saowaluck Pornkulwat, James Lester Figarola, Steven R. Skoda, John E. Foster

John E. Foster

Four hydrolases and five oxido-reductases were examined using native stacking polyacrylamide gel electrophoresis. Homogenate of Russian wheat aphid, Diuraphis noxia (Mordvilko), bird cherry-oat aphid, Rhopalosiphum padi (L.), "Arapahoe" (aphid-susceptible) and "Halt" (aphid-resistant) wheat, Triticum aestivum L., and powdery mildew-infected Erysiphe graminis DC. ex Merat f. sp. tritici Em. Marchal, Arapahoe wheat leaves were assayed for enzyme activities. Pectinesterase, polygalacturonase (or pectinase), cellulase, and amylase activities were examined in the hydrolase group. Catalase, peroxidase, catechol oxidase, superoxide dismutase, and ascorbate oxidase activities were examined in the group of oxido-reductases. The two aphid species had the same hydrolases but different oxido-reductases. Although …


G73-46 Hessian Fly On Wheat, John E. Foster, Gary L. Hein Mar 2012

G73-46 Hessian Fly On Wheat, John E. Foster, Gary L. Hein

John E. Foster

This NebGuide discusses the life cycle, control and prevention of the Hessian fly. Plant-safe dates and resistant wheat varieties are also examined. The Hessian fly, Mayetiola destructor (Say), is not native to the United States, but was probably introduced by Hessian soldiers during the Revolutionary War. This insect was given its common name by Americans because of its damage on Long Island in 1779. The pest has become distributed throughout the United States wheat production areas since then. The Hessian fly belongs to the family of insects known as gall midges (Diptera: Cecidomyiidae), a group noted for their habit of …


Levels Of Tolerance, Antibiosis, And Antixenosis Among Resistant Buffalograsses And Zoysiagrasses, Thomas E. Eickhoff, Tiffany Heng-Moss, Frederick P. Baxendale, John E. Foster Mar 2012

Levels Of Tolerance, Antibiosis, And Antixenosis Among Resistant Buffalograsses And Zoysiagrasses, Thomas E. Eickhoff, Tiffany Heng-Moss, Frederick P. Baxendale, John E. Foster

John E. Foster

The western chinch bug, Blissus occiduus Barber, has been documented as one of the most serious pests of buffalograss, Buchloe dactyloides (Nuttall) Engelmann, and zoysiagrass, Zoysia japonica Steudel, grown for turf in midwestern states. Resistance to the western chinch bug has been identified in both buffalograsses and zoysiagrasses. Choice and no-choice studies were conducted to determine the categories (antibiosis, antixenosis, and tolerance) of three resistant buffalograsses (PX3-5-1’, 196’, and 184’) and three resistant zoysiagrasses (El Toro, Emerald, and Zorro). Antibiosis studies found no significant differences in survival, nymphal development, or fecundity among the resistant and susceptible buffalograsses or zoysiagrasses, indicating …


Differentiation Of Three Phenotypically Similar Blattella Spp.: Analysis With Polymerase Chain Reaction-Restriction Fragment Length Polymorphism Of Mitochondrial Dna, Pari Pachamuthu, Shripat T. Kamble, Thomas L. Clark, John E. Foster Mar 2012

Differentiation Of Three Phenotypically Similar Blattella Spp.: Analysis With Polymerase Chain Reaction-Restriction Fragment Length Polymorphism Of Mitochondrial Dna, Pari Pachamuthu, Shripat T. Kamble, Thomas L. Clark, John E. Foster

John E. Foster

Differentiation of Blattella asahinai Mizukubo, Blattella vaga Hebard, and Blattella germanica (L.) was investigated using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). DNA fragments corresponding to mitochondrial CO1,CO1/TL2(small and large fragments), and ITS2 regions were amplified using PCR. The universal primers used for amplifying mitochondrial CO1 and CO1/TL2 (small and large fragments) were CO1-J-1718/CO1- N-2191, CO1-J-2441/TL2-N-3014, and CO1-J-1751/TL2-N-3014. Amplification was observed with all primer combinations, but diagnostic patterns were found only with AluI digested amplicons of CO1 and CO1/TL2 (large fragments) regions. Both inter/intraspecific polymorphism was observed for the 517 bp CO1 region between German (A, B) and Asian …