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Connexin-32 And Connexin-43 Immunoreactivity In Rodent Taste Buds, Amanda E. Bond
Connexin-32 And Connexin-43 Immunoreactivity In Rodent Taste Buds, Amanda E. Bond
Electronic Theses and Dissertations
Studies indicate that ATP is one of the primary neurotransmitters in taste transduction. ATP release occurs from taste cells via specific hemichannels such as pannexin/connexin hemichannels (Huang et al., 2007; Romanov et al., 2007). We hypothesize that Type II (receptor) and possibly Type III (presynaptic) cells release ATP at sites containing pannexin/connexin hemichannels. In this study, we examine the presence of connexin–32–LIR (Like Immunoreactivity) and connexin–43–LIR in rodent taste buds through immunocytochemical analysis and DAB (Di–amino–benzidine) immunoelectron microscopy. We observed that connexin–32–LIR co–localizes with P2X2–LIR in nerve fibers and in a small subset of NCAM–LIR cells. Connexin–32–LIR does not co–localize …
Analyzing The Interaction Between Melanocortin 5 Receptor, Melanocortin Receptor Accessory Protein 1, And Melanocortin 2 Receptor In Rainbow Trout, Domicinda M. Hill
Analyzing The Interaction Between Melanocortin 5 Receptor, Melanocortin Receptor Accessory Protein 1, And Melanocortin 2 Receptor In Rainbow Trout, Domicinda M. Hill
Electronic Theses and Dissertations
Using CHO cells we sought to explore and characterize the functional relationship of rainbow trout MC5 receptor (rtMC5R) with zebrafish MRAP1 (zfMRAP1) and rainbow trout MC2 receptor (rtMC2R), as well as how the effect of such relationships may play a significant role in Hypothalamic-Pituitary-Adrenal/Interrenal axis activation. This research demonstrated that rtMC5R can be successfully expressed and functionally activated in CHO cells and, in a manner similar to mammals, α-MSH is the preferred ligand for rtMC5R. The presence of MRAP1 does not seem to inhibit the expression or function of rtMC5R, rather it appears that it may increase expression and total …
Fire-Derived Charcoal Along An Ecological Gradient In The Colorado Front Range, Christopher William Licata
Fire-Derived Charcoal Along An Ecological Gradient In The Colorado Front Range, Christopher William Licata
Electronic Theses and Dissertations
Terrestrial ecosystems are shaped by natural disturbances such as wildland fire. In the intermountain western United States, forests, shrub and grasslands adapt to repeated fires. An important long-term legacy of wildland fires is black C (BC) commonly referred to as char or charcoal. Black C is a recalcitrant C form that has been long known to influence soil physical, chemical, and biological processes that they vary across landscapes and over time. The objective of this research is to address two key areas in the emerging field of ecosystem BC research; 1) how much BC as charcoal C is formed per …
Dopamine-Beta-Hydroxylase And Norepinephrine Transporter Immunoreactivity In Rat Taste Buds, Jenna Ashley Walton
Dopamine-Beta-Hydroxylase And Norepinephrine Transporter Immunoreactivity In Rat Taste Buds, Jenna Ashley Walton
Electronic Theses and Dissertations
Of the handful of neurotransmitters modulating taste response, norepinephrine (NE) may be the least understood. Although the physiological role of NE has been tested, it is still unclear whether the rat taste bud may serve as an endogenous source of neurotransmitter or if it must be taken up from outside the taste bud. Immunocytochemical analysis of DBH has shown that DBH–LIR is present in taste cells of the rat circumvallate papillae. Specifically, DBH–LIR is present in a subset of Type II taste cells. Nearly all (98%) DBH–LIR cells express PLCβ2–LIR, and about half (41%) of PLCβ2–LIR cells express DBH–LIR. DBH–LIR …
Nutraceutical Antioxidants And Their Therapeutic Potential In Neurodegeneration, Erika Kristine Ross
Nutraceutical Antioxidants And Their Therapeutic Potential In Neurodegeneration, Erika Kristine Ross
Electronic Theses and Dissertations
Amyotrophic lateral sclerosis (ALS) is a fatal neuromuscular disease that affects motor neurons of the brain and spinal cord. Many studies indicate that mitochondrial oxidative stress (MOS) is a principal mechanism underlying the pathophysiology of this and other devastating neurodegenerative diseases. Here, we investigated a unique whey protein supplement (Immunocal®) to determine its neuroprotective efficacy in several in vitro models of MOS and in an in vivo mouse model of ALS. This non-denatured whey supplement contains cystine which is an oxidized form of cysteine, an essential precursor for synthesis of the endogenous antioxidant, glutathione (GSH). In primary cultured rat cerebellar …