Digital Commons Network^™

Episodic Recruitment And Climate Analysis Of Ponderosa Pine On The Palmer Divide, Eastern Colorado, William Henry Brenton Jr.

Electronic Theses and Dissertations

Previous recruitment studies on populations of ponderosa pine (Pinus ponderosa P. & C. Lawson) in Colorado have been limited to the western mountains and the Front Range. In this research I used tree-ring data to reconstruct recruitment for ponderosa pine near the eastern limits of its distribution at two sites on the Palmer Divide, Eastern Colorado, to determine the relative climate sensitivity of the two sites, and the extent to which climate or other factors may have influenced recruitment at the sites. The results of the tree-ring analysis suggest that ponderosa pines in more the easterly site lower elevation …

Lamination Of Inverted Organic Photovoltaics And Associated Physical Properties, Brian A. Bailey

Electronic Theses and Dissertations

This dissertation is the result of a series of studies involving hot press lamination of inverted organic photovoltaics. It first gives background and develops a lamination process for fabrication. Then, the process is used to answer fundamental questions about organic photovoltaics and to fabricate previously impossible or overly complicated devices. Finally, the author examines the scientific theory which describes the process and aligns that theory with related measurements.

The field of organic photovoltaics is introduced and the lamination process is developed and optimized. In the lamination process, part of the solar cell is deposited onto a rigid substrate, and the …

Design And Synthesis Of Novel Fluorescent Nucleoside Analogues, Nada Abdulnasir Elsharif

Electronic Theses and Dissertations

This project aimed to obtain a set of fluorescent nucleoside analogues that closely resemble cytosine with extending the aromatic surface (extended conjugation) for enhance the photophysical properties. In addition to synthesize new fluorescent nucleoside analogues and evaluate their photophysical properties, this project aimed to understand the effects of donor substituent on the tC photophysical properties.

The main goal of these studies is to develop highly fluorescent nucleotide analogues. We first were looking to synthesize tC^C fluorescent nucleotide with the pyrimido[4,5-b]quinolone. In this part, 2-chloro-3- formylquinoline was easily prepared, yet the cyclization of 2-chloro-3- formylquinoline with urea or guanidine HCl …

Analyzing The Interaction Between Melanocortin 5 Receptor, Melanocortin Receptor Accessory Protein 1, And Melanocortin 2 Receptor In Rainbow Trout, Domicinda M. Hill

Electronic Theses and Dissertations

Using CHO cells we sought to explore and characterize the functional relationship of rainbow trout MC5 receptor (rtMC5R) with zebrafish MRAP1 (zfMRAP1) and rainbow trout MC2 receptor (rtMC2R), as well as how the effect of such relationships may play a significant role in Hypothalamic-Pituitary-Adrenal/Interrenal axis activation. This research demonstrated that rtMC5R can be successfully expressed and functionally activated in CHO cells and, in a manner similar to mammals, α-MSH is the preferred ligand for rtMC5R. The presence of MRAP1 does not seem to inhibit the expression or function of rtMC5R, rather it appears that it may increase expression and total …

Connexin-32 And Connexin-43 Immunoreactivity In Rodent Taste Buds, Amanda E. Bond

Electronic Theses and Dissertations

Studies indicate that ATP is one of the primary neurotransmitters in taste transduction. ATP release occurs from taste cells via specific hemichannels such as pannexin/connexin hemichannels (Huang et al., 2007; Romanov et al., 2007). We hypothesize that Type II (receptor) and possibly Type III (presynaptic) cells release ATP at sites containing pannexin/connexin hemichannels. In this study, we examine the presence of connexin–32–LIR (Like Immunoreactivity) and connexin–43–LIR in rodent taste buds through immunocytochemical analysis and DAB (Di–amino–benzidine) immunoelectron microscopy. We observed that connexin–32–LIR co–localizes with P2X2–LIR in nerve fibers and in a small subset of NCAM–LIR cells. Connexin–32–LIR does not co–localize …

Local Food Actions And Motivations In The Highlands Neighborhood Of Denver, Co, Samantha R. Lester

Electronic Theses and Dissertations

This study evaluates the local food movement within the Highlands neighborhood of Denver, Colorado. There are several potential outlets for local food within the Highlands and it is a diverse demographic community, which creates an interesting and relevant research area. This study evaluates the reasons behind the local food movement through qualitative research of community members, local restaurant owners, community garden members and community organizers as well as through participant observation. The goal of this research is to narrate what one neighborhood within a progressive city is experiencing within the local food movement. The local food movement was found to …

Fire-Derived Charcoal Along An Ecological Gradient In The Colorado Front Range, Christopher William Licata

Electronic Theses and Dissertations

Terrestrial ecosystems are shaped by natural disturbances such as wildland fire. In the intermountain western United States, forests, shrub and grasslands adapt to repeated fires. An important long-term legacy of wildland fires is black C (BC) commonly referred to as char or charcoal. Black C is a recalcitrant C form that has been long known to influence soil physical, chemical, and biological processes that they vary across landscapes and over time. The objective of this research is to address two key areas in the emerging field of ecosystem BC research; 1) how much BC as charcoal C is formed per …

Nutraceutical Antioxidants And Their Therapeutic Potential In Neurodegeneration, Erika Kristine Ross

Electronic Theses and Dissertations

Amyotrophic lateral sclerosis (ALS) is a fatal neuromuscular disease that affects motor neurons of the brain and spinal cord. Many studies indicate that mitochondrial oxidative stress (MOS) is a principal mechanism underlying the pathophysiology of this and other devastating neurodegenerative diseases. Here, we investigated a unique whey protein supplement (Immunocal®) to determine its neuroprotective efficacy in several in vitro models of MOS and in an in vivo mouse model of ALS. This non-denatured whey supplement contains cystine which is an oxidized form of cysteine, an essential precursor for synthesis of the endogenous antioxidant, glutathione (GSH). In primary cultured rat cerebellar …

Dopamine-Beta-Hydroxylase And Norepinephrine Transporter Immunoreactivity In Rat Taste Buds, Jenna Ashley Walton

Electronic Theses and Dissertations

Of the handful of neurotransmitters modulating taste response, norepinephrine (NE) may be the least understood. Although the physiological role of NE has been tested, it is still unclear whether the rat taste bud may serve as an endogenous source of neurotransmitter or if it must be taken up from outside the taste bud. Immunocytochemical analysis of DBH has shown that DBH–LIR is present in taste cells of the rat circumvallate papillae. Specifically, DBH–LIR is present in a subset of Type II taste cells. Nearly all (98%) DBH–LIR cells express PLCβ2–LIR, and about half (41%) of PLCβ2–LIR cells express DBH–LIR. DBH–LIR …

Modeling Of Growth Kinetics And Characterization Of Membrane Mechanics, Antonio Nava

Electronic Theses and Dissertations

One growing field in alternative energy is biofuel production through microorganisms. These fields of research include hydrogen and biofuel production through the cultivation of algae. We've selected 2 different algae, Anabaena sp. cpcc 387 and Tetraselmis to study. Through mathematical modeling of Anabaena we investigate the complex multicellular relationships and colony stability when noise is introduced. In conjunction with researchers at NREL, we've set out to characterize Tetraselmis cells' membrane elasticity.

Analysis Of Interphase Chromatin Motion In Hela Cells, Matthew Joel Westacott

Electronic Theses and Dissertations

Motion of particles under influencing forces may be observed under light microscopy techniques. Variations in mobility of particles may give relevant biophysical information. Automated high resolution single particle tracking techniques were used to characterize interphase chromatin mobility in the cell nucleus. Interphase chromatin undergo replication prior to cell division with the assistance of replication proteins (machinery) which modify chromatin mobility. Using dual color imaging of flourescently tagged chromatin and proliferating cell nuclear antigen (PCNA) were followed through interphase. Chromatin motion was modelled as a two dimensional random walk. Reduction in chromatin mobility was observed during S phase was dependent on …