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Identification Of Cyst Nematodes Of Agronomic And Regulatory Concern With Pcr-Rflp Of Its1, Allen L. Szalanski, Dezhi D. Sui, T. S. Harris, Thomas O. Powers

Department of Plant Pathology: Faculty Publications

The first internally transcribed spacer region (ITS1) from cyst nematode species (Heteroderidae) was compared by nuc.leotide sequencing and PCR-RFLP. European, Asian, and North American isolates of five heterodefid species were examined to assess intraspecific variation. PCR-RFLP patterns of amplified ITS1 DNA from pea cyst nematode, Heterodera goettingiana, from Northern Ireland were identical with patterns from Washington State. Sequencing demonstrated that ITS1 heterogeneity existed within individuals and between isolates, but did not result in different restriction patterns. Three Indian and two U.S. isolates of the corn cyst nematode, Heterodera zeae, were compared. Sequencing detected variation among ITS1 clones from …