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Articles 1 - 13 of 13
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Hyaluronan Synthase Of Chlorella Virus Pbcv-1, Paul L. Deangelis, Wei Jing, Michael V. Graves, Dwight E. Burbank, James L. Van Etten
Hyaluronan Synthase Of Chlorella Virus Pbcv-1, Paul L. Deangelis, Wei Jing, Michael V. Graves, Dwight E. Burbank, James L. Van Etten
Department of Plant Pathology: Faculty Publications
Sequence analysis of the 330-kilobase genome of the virus PBCV-1 that infects a chlorella-like green algae revealed an open reading frame, A98R, with similarity to several hyaluronan synthases. Hyaluronan is an essential polysaccharide found in higher animals as well as in a few pathogenic bacteria. Expression of the A98R gene product in Escherichia coli indicated that the recombinant protein is an authentic hyaluronan synthase. A98R is expressed early in PBCV-1 infection and hyaluronan is produced in infected algae. These results demonstrate that a virus can encode an enzyme capable of synthesizing a carbohydrate polymer and that hyaluronan exists outside of …
Identification Of The Wheat Curl Mite As The Vector Of The High Plains Virus Of Corn And Wheat, Dallas L. Seifers, Tom L. Harvey, T. J. Martin, Stanley G. Jensen
Identification Of The Wheat Curl Mite As The Vector Of The High Plains Virus Of Corn And Wheat, Dallas L. Seifers, Tom L. Harvey, T. J. Martin, Stanley G. Jensen
Department of Plant Pathology: Faculty Publications
Wheat with virus-like symptoms (extracts containing a 33-kDa protein in sodium dodecyl sulfate- polyacrylamide gel electrophoresis, negative in enzyme-linked immunosorbent assay to wheat streak mosaic virus, and not infectious in a backassay to other wheat) reacted positively to antiserum made against a protein purified from symptomatic corn infected with the High Plains virus (HPV), indicating a serological relationship between the corn and wheat pathogens. The wheat curl mite (WCM, Aceria tosichella Keifer) was identified as the vector of the virus and caused persistent infection of barley (Hordeum vulgare L.) and wheat (Triticum aestivum L.) in greenhouse experiments. The …
Characteristics Of Beet Soilborne Mosaic Virus, A Furo-Like Virus Infecting Sugar Beet, G.B. Heidel, C.M. Rush, T.L. Kendall, S.A. Lommel, Roy C. French
Characteristics Of Beet Soilborne Mosaic Virus, A Furo-Like Virus Infecting Sugar Beet, G.B. Heidel, C.M. Rush, T.L. Kendall, S.A. Lommel, Roy C. French
Department of Plant Pathology: Faculty Publications
Beet soilborne mosaic virus (BSBMV) is a rigid rod-shaped virus transmitted by Polymyxa betae. Particles were 19 nm wide and ranged from 50 to over 400 nm, but no consistent modal lengths could be determined. Nucleic acids extracted from virions were polyadenylated and typically separated into three or four discrete bands of variable size by agarose-formaldehyde gel electrophoresis. RNA 1 and 2, the largest of the RNAs, consistently averaged 6.7 and 4.6 kb, respectively. The sizes and number of smaller RNA species were variable. The molecular mass of the capsid protein of BSBMV was estimated to be 22.5 kDa. …
The Aspergillus Nidulans Sepa Gene Encodes An Fh1/2 Protein Involved In Cytokinesis And The Maintenance Of Cellular Polarity, Steven D. Harris, Lisbeth Hamer, Kathryn E. Sharpless, John E. Hamer
The Aspergillus Nidulans Sepa Gene Encodes An Fh1/2 Protein Involved In Cytokinesis And The Maintenance Of Cellular Polarity, Steven D. Harris, Lisbeth Hamer, Kathryn E. Sharpless, John E. Hamer
Department of Plant Pathology: Faculty Publications
Cytokinesis (septation) in the fungus Aspergillus nidulans occurs through the formation of a transient actin ring at the incipient division site. Temperature-sensitive mutations in the sepA gene prevent septation and cause defects in the maintenance of cellular polarity, without affecting growth and nuclear division. The sepA gene encodes a member of the growing family of FH1/2 proteins, which appear to have roles in morphogenesis and cytokinesis in organisms such as yeast and Drosophila Results from temperature shift and immunofluorescence microscopy experiments strongly suggest that sepA function requires a preceding mitosis and that sepA acts prior to actin ring formation. Deletion …
United States Patent, Number: 5,633,447: Plant Tissue Comprising A Subgenomic Promoter, Paul G. Ahlquist, Roy C. French
United States Patent, Number: 5,633,447: Plant Tissue Comprising A Subgenomic Promoter, Paul G. Ahlquist, Roy C. French
Department of Plant Pathology: Faculty Publications
A subgenomic promoter of a positive strand RNA virus is disclosed which directs the amplified expression of a structural gene in plant tissue. The core region and an upstream activating domain of the subgenomic promoter are identified. This promoter can be utilized in a modified virus. or in an appropriate engineered recombinant DNA derivative which may be chromosomally integrated or maintained as an episome in transformed cells.
Chlorella Virus Pbcv-1 Encodes A Homolog Of The Bacteriophage T4 Uv Damage Repair Gene Denv, Masakazu Furuta, John Schrader, Holly Schrader, Tyler Kokjohn, Simon Nyaga, Amanda Mccullough, R. Stephen Lloyd, Dwight Burbank, Dorit Landstein, Leslie C. Lane, James L. Van Etten
Chlorella Virus Pbcv-1 Encodes A Homolog Of The Bacteriophage T4 Uv Damage Repair Gene Denv, Masakazu Furuta, John Schrader, Holly Schrader, Tyler Kokjohn, Simon Nyaga, Amanda Mccullough, R. Stephen Lloyd, Dwight Burbank, Dorit Landstein, Leslie C. Lane, James L. Van Etten
Department of Plant Pathology: Faculty Publications
The bacteriophage T4 denV gene encodes a well-characterized DNA repair enzyme involved in pyrimidine photodimer excision. We have discovered the first homologs of the denV gene in chlorella viruses, which are common in fresh water. This gene functions in vivo and also when cloned in Escherichia coli. Photo-damaged virus DNA can also be photoreactivated by the host chlorella. Since the chlorella viruses are continually exposed to solar radiation in their native environments, two separate DNA repair systems, one that functions in the dark and one that functions in the light, significantly enhance their survival.
Comparison Of Avrd Alleles From Pseudomonas Syringae Pv. Glycinea, Lisa Wolfson Keith, Carol Boyd, Noel T. Keen, J. E. Patridge
Comparison Of Avrd Alleles From Pseudomonas Syringae Pv. Glycinea, Lisa Wolfson Keith, Carol Boyd, Noel T. Keen, J. E. Patridge
Department of Plant Pathology: Faculty Publications
Avirulence gene D alleles resided on indigenous plasmids in races 0, 2, 3, 4, 5, and 6 of Pseudomonas syringae pv. glycinea (Psg), but the allele in race 1 appeared to be chromosomal. These were all nonfunctional avirulence genes because they neither induced the avirulence phenotype on Rpg4 soybean cultivars nor directed the production of syringolide elicitors when expressed in Escherichia coli cells. The predicted proteins encoded by the seven Psg avrD genes were very similar to that of a functional class II allele from P. syringae pv. phaseolicola G50 race 2, but contained mutations collectively …
Characterization Of An Atp-Dependent Dna Ligase Encoded By Chlorella Virus Pbcv-1, C. Kiong Ho, James L. Van Etten, Stewart Shuman
Characterization Of An Atp-Dependent Dna Ligase Encoded By Chlorella Virus Pbcv-1, C. Kiong Ho, James L. Van Etten, Stewart Shuman
Department of Plant Pathology: Faculty Publications
We report that Chlorella virus PBCV-1 encodes a 298-amino-acid ATP-dependent DNA ligase. The PBCV-1 enzyme is the smallest member of the covalent nucleotidyl transferase superfamily, which includes the ATP-dependent polynucleotide ligases and the GTP-dependent RNA capping enzymes. The specificity of PBCV-1 DNA ligase was investigated by using purified recombinant protein. The enzyme catalyzed efficient strand joining on a singly nicked DNA in the presence of magnesium and ATP (Km, 75 μM). Other nucleoside triphosphates or deoxynucleoside triphosphates could not substitute for ATP. PBCV-1 ligase was unable to ligate across a 2-nucleotide gap and ligated poorly across a 1-nucleotide …
United States Patent , Number: 5,602,242: Hybrid Rna Virus, Paul G. Ahlquist, Roy C. French, Robert F. Sacher
United States Patent , Number: 5,602,242: Hybrid Rna Virus, Paul G. Ahlquist, Roy C. French, Robert F. Sacher
Department of Plant Pathology: Faculty Publications
A recombinant RNA virus is provided allowing encapsidation of genetically engineered viral sequences in heterologous, preferably rod-shaped coat, protein capsids. Since icosahedral viruses are limited in the amount of RNA they can carry, and rod-shaped viruses are expansible, this invention allows the size of recombinant virus RNA components to be increased (or decreased). Methods of making and using such recombinant viruses are also provided, specifically with respect to the transfection of plants to bring about genotypic and phenotypic changes.
Genetic Variability Among Maize Inbred Lines For Resistance To The High Plains Virus–Wheat Streak Mosaic Virus Complex, A. Marçon, S. M. Kaeppler, S. G. Jensen
Genetic Variability Among Maize Inbred Lines For Resistance To The High Plains Virus–Wheat Streak Mosaic Virus Complex, A. Marçon, S. M. Kaeppler, S. G. Jensen
Department of Plant Pathology: Faculty Publications
High plains virus (HPV) is a pathogen that causes a severe disease, especially in susceptible corn genotypes. The virus is transmitted by the eriophyid mite Aceria tosichella, which also transmits wheat streak mosaic virus (WSMV). This often results in a mixed infection by these two viruses. Genetic variability for resistance to the HPV exists among maize inbred lines but has not been characterized. The disease reaction of 30 maize inbred lines to the mixed infection and to WSMV alone was characterized in this study. Evaluation was based on symptom expression and virus titer (HPV and WSMV), as measured by …
The Rdna Internal Transcribed Spacer Region As A Taxonomic Marker For Nematodes, Thomas O. Powers, T. C. Todd, A. M. Burnell, P. C. B. Murray, C. C. Flemming, Allen L. Szalanski, B. A. Adams, T. S. Harris
The Rdna Internal Transcribed Spacer Region As A Taxonomic Marker For Nematodes, Thomas O. Powers, T. C. Todd, A. M. Burnell, P. C. B. Murray, C. C. Flemming, Allen L. Szalanski, B. A. Adams, T. S. Harris
Department of Plant Pathology: Faculty Publications
The ITS region from a wide taxonomic range of nematodes, including secernentean and adenophorean taxa, and free-living, entomopathogenic, and plant-parasitic species, was evaluated as a taxonomic marker. Size of the amplified product aided in the initial determination of group membership, and also suggested groups that may require taxonomic reevaluation. Congeneric species often displayed identically sized ITS regions, but genera such as Pratylenchus and Tylenchorhynchus had species with large differences in size. ITS heterogeneity in individuals and populations was identified in several nematode taxa. PCR-RFLP of ITS1 is advocated as a method of taxonomic analysis in genera such as Helicotylenchus that …
The Internal Transcribed Spacer Region Of Belonolaimus (Nemata: Belonolaimidae), T. Cherry, A. L. Szalanski, T. C. Todd, Thomas O. Powers
The Internal Transcribed Spacer Region Of Belonolaimus (Nemata: Belonolaimidae), T. Cherry, A. L. Szalanski, T. C. Todd, Thomas O. Powers
Department of Plant Pathology: Faculty Publications
Belonolaimus isolates from six U.S. states were compared by restriction endonuclease digestion of amplified first internal transcribed spacer region (ITS1) of the nuclear ribosomal genes. Seven restriction enzymes were selected for evaluation based on restriction sites inferred from the nucleotide sequence of a South Carolina Belonolaimus isolate. Amplified product size from individuals of each isolate was approximately 700 bp. All Midwestern isolates gave distinct restriction digestion patterns. Isolates identified morphologically as Belonolaimus longicaudatus from Florida, South Carolina, and Palm Springs, California, were identical for ITS1 restriction patterns. The correlation between ITS1 restriction patterns and the distribution of B. longicaudatus isolates …
Identification Of Cyst Nematodes Of Agronomic And Regulatory Concern With Pcr-Rflp Of Its1, Allen L. Szalanski, Dezhi D. Sui, T. S. Harris, Thomas O. Powers
Identification Of Cyst Nematodes Of Agronomic And Regulatory Concern With Pcr-Rflp Of Its1, Allen L. Szalanski, Dezhi D. Sui, T. S. Harris, Thomas O. Powers
Department of Plant Pathology: Faculty Publications
The first internally transcribed spacer region (ITS1) from cyst nematode species (Heteroderidae) was compared by nuc.leotide sequencing and PCR-RFLP. European, Asian, and North American isolates of five heterodefid species were examined to assess intraspecific variation. PCR-RFLP patterns of amplified ITS1 DNA from pea cyst nematode, Heterodera goettingiana, from Northern Ireland were identical with patterns from Washington State. Sequencing demonstrated that ITS1 heterogeneity existed within individuals and between isolates, but did not result in different restriction patterns. Three Indian and two U.S. isolates of the corn cyst nematode, Heterodera zeae, were compared. Sequencing detected variation among ITS1 clones from …