Digital Commons Network^™

Visual And Somatosensory Contributions To Infant Sitting Postural Control, Anastasia Kyvelidou, Nicholas Stergiou

Journal Articles

There are a limited number of studies that have investigated sitting posture during infancy and the contribution of the sensory systems. The goal of this study was to examine the effects of altered visual and somatosensory signals on infant sitting postural control. Thirteen infants (mean age ± SD, 259.69 ± 16.88 days) participated in the study. Initially, a single physical therapist performed the Peabody Developmental Motor Scale to determine typical motor development. Then the child was placed onto a force platform under four randomized conditions: (a) Control (C) – sat independently on the force plate, (b) Somatosensory (SS) – Sat …

Ne Stem 4u Afterschool Intervention Leads To Gains In Stem Content Knowledge For Middle School Youth, Christine E. Cutucache, Taylor Boham, Jamie Luhr, Amie Sommers, Nikolaus Stevenson, Erkko Sointu, Kati Mäkitalo‐Siegl, Sirpa Kärkkäinen, Teemu Valtonen, Neal Grandgenett, William Tapprich

Biology Faculty Publications

Afterschool interventions in STEM are linked to learning gains during the school day. These opportunities engage and excite students about STEM concepts since they observe a more hands-on, project-oriented approach. Often these opportunities for afterschool interventions are infrequent in nature and leave gaps for students in their maturation and understanding. Herein we describe the first report of an afterschool intervention, named NE STEM 4U, targeting socioeconomically disadvantaged middle school youth via a twice weekly, year-long intervention, studied across two years. We assessed the impact of this program on i.) short-term, individual student gains in STEM content knowledge and ii.) delivery …

The Effects Of A 12-Week Jump Rope Exercise Program On Abdominal Adiposity, Vasoactive Substances, Inflammation, And Vascular Function In Adolescent Girls With Prehypertension, Ki-Dong Sung, Elizabeth J. Pekas, Steven D. Scott, Won-Mok Son, Song-Young Park

Health and Kinesiology Faculty Publications

Introduction

Childhood obesity is strongly associated with cardiovascular disease (CVD) development. It is necessary to combat unfavorable outcomes of obesity at a young age by utilizing effective interventions, such as exercise.

Purpose

We sought to examine the effects of a jump rope exercise program on CVD risk factors, including body composition, vasoactive substances, inflammation, and vascular function in prehypertensive adolescent girls.

Methods

Forty girls (age 14–16) were recruited and randomly assigned to a jump rope exercise group (EX, n = 20) or control group (CON, n = 20). Body composition, nitrate and nitrite levels, endothelin-1 (ET-1), C-reactive protein (CRP), systolic …

Ask The Beasts Of The Southern Wild: Exploring Human Identity As Beast, Being And Beholder In Ask The Beasts: Darwin And The God Of Love And Beasts Of The Southern Wild, Stephanie Cherpak Clary

Journal of Religion & Film

Anthropocentrism and hierarchical dualism together encourage a dangerous anthropology where human primacy among creation and the prioritization of certain humans leads to destruction for all. During a time when suffering caused by climate change continues to intensify, it is increasingly important to find compelling ways to share the stories of those who suffer most. I will explore how Beasts of the Southern Wild (Zeitlin, 2012) contextualizes the ecofeminist theology found in Johnson’s Ask the Beasts: Darwin and the God of Love (2014), specifically the idea of humans identifying as beasts, beings and beholders. Furthermore, I will discuss how the representation …

Lower Limb Joint Angle Variability And Dimensionality Are Different In Stairmill Climbing And Treadmill Walking, Peter C. Raffalt, Srikant Vallabhajosula, Jessica Renz, Mukul Mukherjee, Nicholas Stergiou

Journal Articles

The present study tested if the quadratic relationship which exists between stepping frequency and gait dynamics in walking can be generalized to stairmill climbing. To accomplish this, we investigated the joint angle dynamics and variability during continuous stairmill climbing at stepping frequencies both above and below the preferred stepping frequency (PSF). Nine subjects performed stairmill climbing at 80, 90, 100, 110 and 120% PSF and treadmill walking at preferred walking speed during which sagittal hip, knee and ankle angles were extracted. Joint angle dynamics were quantified by the largest Lyapunov exponent (LyE) and correlation dimension (CoD). Joint angle variability was …

Acute High-Intensity Exercise Impairs Skeletal Muscle Respiratory Capacity, Gwenael Layec, Gregory M. Blain, Matthew J. Rossman, Song-Young Park, Corey R. Hart, Joel D. Trinity, Jayson R. Gifford, Simranjit K. Sidhu, Joshua C. Weavil, Thomas J. Hureau, Markus Amann, Russell S. Richardson

Health and Kinesiology Faculty Publications

Purpose

The effect of an acute bout of exercise, especially high-intensity exercise, on the function of mitochondrial respiratory complexes is not well understood, with potential implications for both the healthy population and patients undergoing exercise-based rehabilitation. Therefore, this study sought to comprehensively examine respiratory flux through the different complexes of the electron transport chain in skeletal muscle mitochondria before and immediately after high-intensity aerobic exercise.

Methods

Muscle biopsies of the vastus lateralis were obtained at baseline and immediately after a 5-km time trial performed on a cycle ergometer. Mitochondrial respiratory flux through the complexes of the electron transport chain was …

Phr: Patient Health Record, Quinn Nelson

Theses/Capstones/Creative Projects

The rapid development of information technology systems has expanded into multiple disciplines and results in systems that are limited by initial design and implementation: the Healthcare Information Technology (HIT) space is no different. The introduction of the Electronic Health Record (EHR) system has changed the way healthcare operates. Initial designs of these systems were focused on serving the needs of insurance companies and healthcare billing departments. Research shows that the design of EHR systems negatively impact provider-patient interactions and the care they receive. This capstone project capitalizes on the collaboration efforts between UNO and UNMC – by joining a research …

Vascular Mitochondrial Respiratory Function: The Impact Of Advancing Age, Soung Hun Park, Oh-Sung Kwon, Song-Young Park, Joshua C. Weavil, Robert H. I. Andtbacka, John R. Hyngstrom, Van Reese, Russell S. Richardson

Health and Kinesiology Faculty Publications

Little is known about vascular mitochondrial respiratory function and the impact of age. Therefore, skeletal muscle feed arteries were harvested from young (33 ± 7 yr, n = 10), middle-aged (54 ± 5 yr, n = 10), and old (70 ± 7 yr, n = 10) subjects, and mitochondrial respiration as well as citrate synthase (CS) activity were assessed. Complex I (CI) and complex I + II (CI+II) state 3 respiration were greater in young (CI: 10.4 ± 0.8 pmol·s−1·mg−1 and CI+II: 12.4 ± 0.8 pmol·s−1·mg−1, P < 0.05) than middle-aged (CI: 7 ± 0.6 pmol·s−1·mg−1 and CI+II: 8.3 ± 0.5 pmol·s−1·mg−1) and old (CI: 7.2 ± 0.4 pmol·s−1·mg−1 and CI+II: 7.6 ± 0.5 pmol·s−1·mg−1) subjects and, as in the case of complex II (CII) state 3 respiration, were inversely correlated with age [r = −0.56 (CI), r = −0.7 (CI+II), and r = 0.4 (CII), …

When Coordinating Finger Tapping To A Variable Beat The Variability Scaling Structure Of The Movement And The Cortical Bold Signal Are Both Entrained To The Auditory Stimuli, Steven Harrison, Michael Hough, Kendra K. Schmid, Boman Groff, Nicholas Stergiou

Journal Articles

Rhythmic actions are characterizable as a repeating invariant pattern of movement together with variability taking the form of cycle-to-cycle fluctuations. Variability in behavioral measures is atypically random, and often exhibits serial temporal dependencies and statistical self-similarity in the scaling of variability magnitudes across timescales. Self-similar (i.e. fractal) variability scaling is evident in measures of both brain and behavior. Variability scaling structure can be quantified via the scaling exponent (α) from detrended fluctuation analysis (DFA). Here we study the task of coordinating thumb-finger tapping to the beats of constructed auditory stimuli. We test the hypothesis that variability scaling evident …

Derivatives Of A Benzoquinone Acyl Hydrazone With Activity Against Toxoplasma Gondii, A. G. Sanford, T. T. Schulze, L. P. Potluri, G. F. Watson, E. B. Darner, S. J. Zach, R. M. Hemsley, A. I. Wallick, R. C. Warner, S. A. Charman, X. Wang, J. L. Vennerstrom, P. H. Davis

Biology Faculty Publications

Toxoplasma gondii is an obligate intracellular parasite with global incidence. The acute infection, toxoplasmosis, is treatable but current regimens have poor host tolerance and no cure has been found for latent infections. This work builds upon a previous high throughput screen which identified benzoquinone acyl hydrazone (KG8) as the most promising compound; KG8 displayed potent in vitro activity against T. gondii but only marginal in vivoefficacy in a T. gondii animal model. To define the potential of this new lead compound, we now describe a baseline structure-activity relationship for this chemotype. Several derivatives displayed IC50's comparable …

Sampling Frequency Influences Sample Entropy Of Kinematics During Walking, Peter C. Raffalt, John D. Mccamley, William Denton, Jennifer M. Yentes

Journal Articles

Sample entropy (SaEn) has been used to assess the regularity of lower limb joint angles during walking. However, changing sampling frequency and the number of included strides can potentially affect the sample entropy. The present study investigated the effect of sample frequency and the number of included strides on the calculations of SaEn in joint angle signals recorded during treadmill walking. Eleven subjects walked at their preferred walking speed for 10 minutes, and SaEn was calculated on sagittal plane hip, knee and ankle angle signals extracted from 50, 100, 200, 300 and 400 strides at sampling frequencies of 60, 120, …

Taekwondo Training Reduces Blood Catecholamine Levels And Arterial Stiffness In Postmenopausal Women With Stage-2 Hypertension: Randomized Clinical Trial, Sang-Ho Lee, Steven D. Scott, Elizabeth J. Pekas, Seungyong Lee, Seok Hoon Lee, Song-Young Park

Health and Kinesiology Faculty Publications

Objective: Menopause is associated with a progressive impairment of vascular function and muscular strength in women. Accordingly, the purpose of this study was to determine if Taekwondo training could improve blood catecholamine levels, arterial stiffness, blood pressure (BP) and skeletal muscle strength in postmenopausal women with stage-2 hypertension.

Methods: 20 postmenopausal women (70 ± 4 years old) with stage-2 hypertension were randomly assigned to a 1) Taekwondo training (TT; n = 10) or 2) Control (CON; n = 10) group. Taekwondo training was performed for 60 minutes/day, 3 days/week for 12-weeks.

Results: There were significant (P …

Recruitment, Survival, And Parasitism Of Monarch Butterflies (Danaus Plexippus) In Milkweed Gardens And Conservation Areas, Emily A. Geest, L. Lareesa Wolfenbarger, John P. Mccarty

Biology Faculty Publications

Monarch butterflies (Danaus plexippus) are suffering from declining populations and conservationists have encouraged planting milkweed gardens in urban and suburban landscapes to help offset habitat loss across the breeding range. The effectiveness of gardens as a conservation strategy depends on their ability to attract ovipositing adults and the survival of monarch larvae in these gardens. Larvae are susceptible to a variety of predators as well as to parasitism by a tachinid fly (Lespesia archippivora) and a protozoan parasite (Ophryocystis elektroscirrha) which cause lethal or sublethal effects, yet the severity of these risks in gardens …

Optic Flow Improves Step Width And Length In Older Adults While Performing Dual Task, Taylor Leeder, Farah Fallahtafti, Molly Schieber, Sara A. Myers, Julie Blaskewicz Boron, Jennifer M. Yentes

Journal Articles

Background

Dual-task paradigms are used to investigate gait and cognitive declines in older adults (OA). Optic-flow is a virtual reality environment where the scene flows past the subject while walking on a treadmill, mimicking real-life locomotion.

Aims

To investigate cost of environment (no optic-flow v. optic-flow) while completing single- and dual-task walking and dual-task costs (DTC; single- v. dual-task) in optic-flow and no optic-flow environments.

Methods

Twenty OA and seven younger adults (YA) walked on a self-paced treadmill in 3-min segments per task and both environments. Five task conditions included: no task, semantic fluency (category), phonemic fluency (letters), word reading, …

Altering Gait Variability With An Ankle Exoskeleton, Prokopios Antonellis, Samuel Galle, Dirk De Clercq, Philippe Malcolm

Journal Articles

Exoskeletons can influence human gait. A healthy gait is characterized by a certain amount of variability compared to a non-healthy gait that has more inherent variability; however which exoskeleton assistance parameters are necessary to avoid increasing gait variability or to potentially lower gait variability below that of unassisted walking are unknown. This study investigated the interaction effects of exoskeleton timing and power on gait variability. Ten healthy participants walked on a treadmill with bilateral ankle-foot exoskeletons under ten conditions with different timing (varied from 36% to 54% of the stride) and power (varied from 0.2 to 0.5 W∙kg-1) combinations. We …

Life Science Undergraduate Mentors In Ne Stem 4u Significantly Outperform Their Peers In Critical Thinking Skills, Kari L. Nelson, Claudia M. Rauter, Christine E. Cutucache

Biology Faculty Publications

The development of critical thinking skills in recent college graduates is keenly requested by employers year after year. Moreover, improving these skills can help students to better question and analyze data. Consequently, we aimed to implement a training program that would add to the critical thinking skills of undergraduate students: Nebraska Science, Technology, Engineering, and Math 4U (NE STEM 4U). In this program, undergraduates provide outreach, mentoring, and science, technology, engineering, and mathematics (STEM) education to K–8 students. To determine the impacts of serving as an undergraduate mentor in this program on critical thinking, we compared undergraduate mentors (intervention group) …

Orderliness Of Visual Stimulus Motion Mediates Sensorimotor Coordination, Joshua L. Haworth, Nicholas Stergiou

Journal Articles

We explored the coupling of gaze and postural sway to the motion of a visual stimulus, to further understand sensorimotor coordination. Visual stimuli consisted of a horizontally oscillating red dot, moving with periodic (sine), chaotic, or aperiodic (brown noise) temporal structure. Cross Recurrence Quantification Analysis (cRQA) was used to investigate the coupling between each measured signal with the time series of the visual stimulus position. The cRQA parameter of percent determinism indicated similar strength of coupling of gaze with either periodic or chaotic motion structures, yet weaker coupling to aperiodic stimulus motion. The cRQA parameter of Maxline indicated a particular …

On The Choice Of Multiscale Entropy Algorithm For Quantification Of Complexity In Gait Data, Peter C. Raffalt, William Denton, Jennifer M. Yentes

Journal Articles

The present study aimed at identifying a suitable multiscale entropy (MSE) algorithm for assessment of complexity in a stride-to-stride time interval time series. Five different algorithms were included (the original MSE, refine composite multiscale entropy (RCMSE), multiscale fuzzy entropy, generalized multiscale entropy and intrinsic mode entropy) and applied to twenty iterations of white noise, pink noise, or a sine wave with added white noise. Based on their ability to differentiate the level of complexity in the three different generated signal types, and their sensitivity and parameter consistency, MSE and RCMSE were deemed most appropriate. These two algorithms were applied to …

On The Calculation Of Sample Entropy Using Continuous And Discrete Human Gait Data, John D. Mccamley, William Denton, Peter C. Raffalt, Jennifer M. Yentes

Journal Articles

Sample entropy (SE) has relative consistency using biologically-derived, discrete data >500 data points. For certain populations, collecting this quantity is not feasible and continuous data has been used. The effect of using continuous versus discrete data on SE is unknown, nor are the relative effects of sampling rate and input parameters m (comparison vector length) and r(tolerance). Eleven subjects walked for 10-minutes and continuous joint angles (480 Hz) were calculated for each lower-extremity joint. Data were downsampled (240, 120, 60 Hz) and discrete range-of-motion was calculated. SE was quantified for angles and range-of-motion at all sampling rates and multiple combinations …

Stride-To-Stride Fluctuations In Transtibial Amputees Are Not Affected By Changes In Push-Off Mechanics From Using Different Prostheses, Chase G. Rock, Shane R. Wurdeman, Nikolas Stergiou, Kota Z. Takahashi

Journal Articles

Stride-to-stride fluctuations of joint kinematics during walking reflect a highly structured organization that is characteristic of healthy gait. The organization of stride-to-stride fluctuations is disturbed in lower-limb prosthesis users, yet the factors contributing to this difference are unclear. One potential contributor to the changes in stride-to-stride fluctuations is the altered push-off mechanics experienced by passive prosthesis users. The purpose of our study was to determine if changes in push-off mechanics affect stride-to-stride fluctuations in transtibial amputees. Twenty-two unilateral transtibial amputees were enrolled in the 6- week cross-over study, where High and Low Activity (based on the Medicare Functional Classification System) …

The Relationship Between Acute Pain And Dynamic Postural Stability Indices In Individuals With Patellar Tendinopathy, Adam B. Rosen, Jupil Ko, Cathleen N. Brown

Health and Kinesiology Faculty Publications

Background

Patellar tendinopathy is a common condition resulting in persistent pain, frequently reported during physical activity. The relationship between dynamic postural stability and pain in these individuals is unclear and how it may affect postural stability.

Research Question

Is there a relationship between acute pain and dynamic postural stability indices in individuals with patellar tendinopathy?

Methods

Twenty-two recreationally active individuals with patellar tendinopathy participated. Participants performed a two-legged jump and landed on a single test-limb on a force platform. They completed 100 mm visual analogue scales (VAS) before and after landing trials. Anterior-posterior (APSI), medial-lateral (MLSI), vertical (VSI), and composite …

Interferon Priming Is Essential For Human Cd34+ Cell-Derived Plasmacytoid Dendritic Cell Maturation And Function, A. Lausten, R. O. Bak, C. Krapp, L. Kjær, J. H. Egedahl, C. C. Petersen, S. Pillai, H. Q. Tang, N. Uldbjerg, M. Porteus, N. R. Roan, M. Nyegaard, Paul W. Denton, M. R. Jakobsen

Biology Faculty Publications

Plasmacytoid dendritic cells (pDC) are essential for immune competence. Here we show that pDC precursor differentiated from human CD34+ hematopoietic stem and progenitor cells (HSPC) has low surface expression of pDC markers, and has limited induction of type I interferon (IFN) and IL-6 upon TLR7 and TLR9 agonists treatment; by contrast, cGAS or RIG-I agonists-mediated activation is not altered. Importantly, after priming with type I and II IFN, these precursor pDCs attain a phenotype and functional activity similar to that of peripheral blood-derived pDCs. Data from CRISPR/Cas9-mediated genome editing of HSPCs further show that HSPC-pDCs with genetic modifications can be …

Repeatability And Reliability Of Exploratory Behavior In Proactive And Reactive Zebrafsh, Danio Rerio, Matthew R. Baker, Alexander C. Goodman, Jonathan B. Santo, Ryan Y. Wong

Biology Faculty Publications

Behavioral responses to novel situations often vary and can belong to a suite of correlated behaviors. Characteristic behaviors of different personality types (e.g. stress coping styles) are generally consistent across contexts and time. Here, we compare the repeatability and reliability of exploratory behaviors between zebrafsh strains selectively bred to display contrasting behavioral responses to stressors that represent the proactive-reactive axis. Specifically, we measure exploratory behavior of individual fish in an open field test over five weeks. We quantified the stationary time, average swimming speed and time spent by a fish in the center area. We found a number of strain …

Quantification Of Daily Physical Activity And Sedentary Behavior Of Claudicating Patients, Hernan Hernandez, Sara A. Myers, Molly Schieber, Duy M. Ha, Sarah Baker, Panagiotis Koutakis, Kyung-Soo Kim, Constance Mietus, George P. Casale, Iraklis Pipinos

Journal Articles

Background

Methods

A Novel And Safe Approach To Simulate Cutting Movements Using Ground Reaction Forces, Amelia Lanier, Brian Knarr, Nicholas Stergiou, Thomas S. Buchanan

Journal Articles

Control of shear ground reaction forces (sGRF) is important in performing running and cutting tasks as poor sGRF control has implications for those with knee injuries, such as anterior cruciate ligament (ACL) ruptures. The goal of this study was to develop a novel and safe task to evaluate control or accurate modulation of shear ground reaction forces related to those generated during cutting. Our approach utilized a force control task using real-time visual feedback of a subject’s force production and evaluated control capabilities through accuracy and divergence measurements. Ten healthy recreational athletes completed the force control task while force control …

Exoskeleton Assistance Symmetry Matters: Unilateral Assistance Reduces Metabolic Cost, But Relatively Less Than Bilateral Assistance, Philippe Malcolm, Samuel Galle, Pieter Van Den Berghe, Dirk De Clercq

Journal Articles

Background: Many gait impairments are characterized by asymmetry and result in reduced mobility. Exoskeletons could be useful for restoring gait symmetry by assisting only one leg. However, we still have limited understanding of the effects of unilateral exoskeleton assistance. Our aim was to compare the effects of unilateral and bilateral assistance using a within-subject study design.

Methods: Eleven participants walked in different exoskeleton conditions. In the Unilateral conditions, only one leg was assisted. In Bilateral Matched Total Work, half of the assistance from the Unilateral conditions was applied to both legs such that the bilateral sum was equal …

Functional Performance Deficits In Adolescent Athletes With A History Of Lateral Ankle Sprain(S), Jupil Ko, Adam B. Rosen, Cathleen N. Brown

Health and Kinesiology Faculty Publications

Objective

To determine if adolescent athletes with a history of lateral ankle sprain(s) (LAS) displayed deficits on functional performance tests (FPTs) and if deficits on FPT were related to the number of previous LAS.

Design

Cross-sectional study.

Setting

Biomechanics Laboratory.

Participants

The injured group (n = 24) had a history of ≥1 moderate-severe LAS. The uninjured group (n = 34) had no history of LAS.

Main outcome measure(s)

The average reach distance of three trials in each direction of the star excursion balance test (SEBT) was normalized to leg length (%). The average of two trials of single-leg-hop test (SLHT) …

Deciphering Splenic Marginal Zone Lymphoma Pathogenesis: The Proposed Role Of Microrna, Jacob E. Robinson, Christine E. Cutucache

Biology Faculty Publications

Splenic marginal zone lymphoma (SMZL) is a malignancy of mature B-cells that primarily involves the spleen, but can affect peripheral organs as well. Even though SMZL is overall considered an indolent malignancy, the majority of cases will eventually progress to be more aggressive. In recent years, the gene expression profile of SMZL has been characterized in an effort to identify: 1) the etiology of SMZL, 2) biological consequences of SMZL, and 3) putative therapeutic targets. However, due to the vast heterogeneity of the malignancy, no conclusive target(s) have been deciphered. However, the role of miRNA in SMZL, much as it …

Socioeconomic Status And Cigarette Expenditure Among Us Households: Results From 2010 To 2015 Consumer Expenditure Survey, Mohammed Siahpush, Paraskevi A. Farazi, Shannon I. Maloney, Danae Dinkel, Minh N. Nguyen, Gopal K. Singh

Health and Kinesiology Faculty Publications

Objectives To examine (1) the association between household socioeconomic status (SES) and whether a household spends money on cigarettes and (2) socioeconomic variations in proportion of total household expenditure spent on cigarettes among smoking households.

Methods We pooled data from six consecutive years, 2010–2015, of the Consumer Expenditure Interview Survey. The interviews involved a structured questionnaire about household income, demographics and expenditures including expenditure on cigarettes. Households that reported cigarette expenditure in the previous 3 months were distinguished as smoking households. SES indicators were household poverty status, education and occupation of the head of household. Logistic regression was used to …

Comparison Of Wearable Trackers’ Ability To Estimate Sleep, Jung-Min Lee, Wonwoo Byun, Alyssa Keill, Danae Dinkel, Yaewon Seo

Health and Kinesiology Faculty Publications

Tracking physical activity and sleep patterns using wearable trackers has become a current trend. However, little information exists about the comparability of wearable trackers measuring sleep. This study examined the comparability of wearable trackers for estimating sleep measurement with a sleep diary (SD) for three full nights. A convenience sample of 78 adults were recruited in this research with a mean age of 27.6... 11.0 years. Comparisons between wearable trackers and sleep outcomes were analyzed using the mean absolute percentage errors, Pearson correlations, Bland–Altman Plots, and equivalent testing. Trackers that showed the greatest equivalence with the SD for total sleep …