Digital Commons Network^™

Characterizing The Role Of Pa5189 Of Pseudomonas Aeruginosa In Deletion And Overexpression Mutants, Seh Na Mellick

Theses/Capstones/Creative Projects

In the context of rising multidrug resistance in biofilm-forming pathogens like Pseudomonas aeruginosa, this study investigates the role of the understudied transcription factor PA5189 in antibiotic resistance and biofilm formation. PA5189 deletion and overexpression mutants were created in a parent P. aeruginosa strain using pEX18Tc-based recombinant suicide vectors, with genotypic verification of putative triparental conjugants achieved through restriction digestion and PCR. The study revealed that PA5189 overexpression significantly increases resistance to commonly used broad spectrum antibiotics such as ciprofloxacin and imipenem. Additionally, differential expression of PA5189 was found to notably affect biofilm formation, with variations contingent on the nutrient …

Addressing Athletes Coexisting Conditions Of Autism And Depression: Participants Self-Report Decreased Feelings Of Depression Post Exercise, Joshua Sutton, Saundra Shillingstad, Jessica Baldwin

Theses/Capstones/Creative Projects

The goal of this study was to evaluate if exercise would decrease depressive symptoms of participants who reported having coexisting conditions of autism spectrum disorder (ASD) and depression. Research has reported that autistic people have increased rates of depression, in comparison to general populations (Unruh et al., 2020). The purpose of this research was to evaluate if exercise would decrease depressive symptoms of the Unified Fitness athlete participants who self-identified as having depressive symptoms and ASD. This research was conducted during the Fall 2023 semester as an extension of the class Kinesiology Practicum, using the University of Nebraska Omaha (UNO) …

Effects Various Distilled Water Brands Have On Chlorophytum Comosum., Brooke Bluhm

Theses/Capstones/Creative Projects

When first attempting home gardening, one of the challenges many home-renters are starting to face is being able to supply enough clean water to one’s plants. In order to overcome this challenge, many plant enthusiasts are utilizing prepackaged distilled water from price friendly brands available at most regional Walmart locations. With the number of choices available on the shelves of today’s grocery stores, and vague ingredient lists on packaging it can be challenging to determine which brand is actually best suited for one’s specific needs. In order to determine the best distilled water suited to grow Chlorophytum comosum, various gallons …

Analysis Of Water Pollutants Across Greater Omaha, Desmond Marshall

Theses/Capstones/Creative Projects

Water is a finite resource, and most water has the potential to become contaminated in sitting bodies. Health complications have an increased chance of occurring when these water sources have pollutants. Preventative and treatment methods are components of the legislation put in place to prevent concentrations from reaching levels capable of causing these ailments. However, sites sometimes fail to keep them under tolerated levels. This study is intended to examine if several locations across Omaha are keeping pollutants under legal concentrations and analyze any trends in the data. Locations (n=10) are sampled twice, with exactly two-weeks between collections. The water …

Optimization Of Tyrosine Hydroxylase Antibody For Immunohistochemistry Fluorescence Detection In Zebrafish (Danio Rerio), Madison Thurber

Theses/Capstones/Creative Projects

Dopamine is an important neurotransmitter produced through the catecholamine synthesis pathway that affects brain activity. Unregulated dopamine levels can lead to various diseases such as Parkinson’s Disease or attention deficit hyperactivity disorder (ADHD). Optimization of an immunohistochemistry protocol will allow for the quantification of tyrosine hydroxylase antibody, which indirectly allows for dopamine quantification in dopaminergic regions within the brain. However, the antibody concentration to give the optimal signal-to-noise ratio in IHC varies across studies. Through this experiment, I determined the concentration of tyrosine hydroxylase (TyrH) antibody for immunohistochemistry that gave the best signal-to-background noise ratio within several known dopaminergic regions …

A Phylogenetic Analysis Of The Population Dynamics Of A Captive Colony Of Diploptera Punctata., Nick Peterson

Theses/Capstones/Creative Projects

The unique milk production of Diploptera punctata makes this species a promising model species in entomology for studying the evolution of milk production and for insect endocrinology. Despite this, the genetic diversity of captive populations of the species is not well studied. To better evaluate the captive diversity and population dynamics of Diploptera punctata at UNO we extracted the DNA of 20 random individuals and amplified their DNA using 4 different primers before cleaning the amplified products and sequencing the genes of the samples for use in both individual and concatenated phylogenetic trees. Our results indicate the UNO colony of …

The Effect Of An Oak Savanna Habitat Restoration On Bat Activity In Eastern Nebraska, Aidan Hatfield, Jeremy White

Theses/Capstones/Creative Projects

Oak savanna habitat has been drastically reduced throughout the Midwest. Temperate bats generally are edge adapted and may better utilize a restored oak savanna habitat than the now common deciduous forests that replaced them. Therefore, we analyzed the activity of 7 bats native to eastern Nebraska and in the process, compared the agreement of two different programs that claim to automatically identify bat species. In order to investigate this, we placed three audio detectors within both habitats for 1 week and allowed them to record from sunset to sunrise. The data obtained was analyzed and compared between two programs, Kaleidoscope …

Lower Body Gait Variability As A Distinguishing Feature In Humans, Tyler M. Wiles, Seung Kyeom Kim, Nick Stergiou, Aaron D. Likens

UNO Student Research and Creative Activity Fair

Most humans have a fingerprint that is unique and persists throughout life. The same may be true for gait. Each person is unique physiologically and biomechanically, and has experienced a unique progression of life including injuries, habits, hobbies, or lifestyles that all contribute to navigating the world. Those individualized experiences, whether intended or not, are potentially on display in gait. We hypothesized that there are gait characteristics intrinsic and unique to everyone, so that everyone has a unique “gaitprint”, similar to humans possessing unique fingerprints. To test our hypothesis, we recruited thirty healthy young adults between the ages 19-35 as …

Functional Outcomes Of Noninvasive Sensory Feedback In Upper Limb Prostheses: A Meta-Analysis, Libby Caldwell

UNO Student Research and Creative Activity Fair

Noninvasive sensory feedback applies vibration or pressure to the residual limb and is thought to improve the functionality of upper limb prostheses. However, the efficacy of this approach has not been sufficiently investigated, particularly regarding actual amputee efficacy. The purpose of this study was to evaluate the functional benefits of noninvasive sensory feedback in upper limb prostheses through a meta-analysis. Electronic databases (MEDLINE, Web of Science, and PubMed) were searched up to October 2022 using the following search terms: (prosthesis OR prosthetic) AND (hand OR arm OR forearm OR transradial OR “upper limb” OR “upper extremity) AND (tactile OR haptic …

Walking With A Load On Wheels: Potential Effects Of Bidirectional Springs On Walking Economy, Hiva Razavi, Philippe Malcolm

UNO Student Research and Creative Activity Fair

Walking with a Load on Wheels: Potential Effects of Bidirectional Springs on Walking Economy

Have you ever noticed how your arms alternatively flex and extend when pushing a heavy shopping cart? Accelerating and decelerating heavy masses, such as a shopping cart, can require much effort. During walking, we alternatively accelerate and decelerate our Center of Mass (COM) in every gait cycle, which can require even more effort if we accelerate and decelerate a load while walking. Theoretically, this effort can be reduced by timing the acceleration and deceleration of the load's mass opposite to the individual's COM. Previous studies have …

Materials And Methods Developed For The Recording And Analysis Of Behavior In The Common Marmoset (Callithrix Jacchus)., Christian Wintle

UNO Student Research and Creative Activity Fair

Materials and methods developed for the recording and analysis of behavior in the common marmoset (Callithrix jacchus).

Christian J. Wintle^a, Jordan B. Hernandez^a,b,c, Dobromir Dotov^d, and Jonathan B. Clayton^a,b,e,f,g

^aDepartment of Biology, University of Nebraska at Omaha, Omaha, NE, USA

^bNebraska Food for Health Center, University of Nebraska-Lincoln, Lincoln, NE, USA

^cDepartment of Genetics, Cell Biology, and Anatomy, University of Nebraska Medical Center, NE, USA

^dDepartment of Biomechanics, University of Nebraska at Omaha, Omaha, NE, USA

^eDepartment of Food Science and Technology, University of Nebraska-Lincoln, Lincoln, …

Inter-Joint Variability And Age-Related Changes In Human Walking, Mehrnoush Haghighatnejad, Tyler Wiles, Seung Kyeom Kim, Aaron Likens, Nicholas Stergiou

UNO Student Research and Creative Activity Fair

Inter-Joint Variability and Age-Related Changes in Human Walking

Mehrnoush Haghighatnejad¹*, Tyler M. Wiles¹, Seung Kyeom Kim¹, Nick Stergiou^1,2, Aaron D. Likens¹

¹Department of Biomechanics, University of Nebraska at Omaha

²Department of Physical Education and Sport Science, Aristotle University, Thessaloniki, Greece

*Corresponding author’s email: mhaghighatnejad@unomaha.edu

Presentation Preference: Poster

Humans routinely perform a variety of activities such as walking, running, driving, and typing, all of which require coordination among different body joints. For instance, walking and running involve rhythmic movements of the arms and legs. The involvement of the upper …

Negative Impact Of Aging On Gait Autocorrelation: A Mixed-Effects Analysis Of Stride Interval Dynamics, Theodore Deligiannis, Tyler M. Wiles, Seung Kyeom Kim, Nikolaos Stergiou, Aaron Likens

UNO Student Research and Creative Activity Fair

NEGATIVE IMPACT OF AGING ON GAIT AUTOCORRELATION: A MIXED-EFFECTS ANALYSIS OF STRIDE INTERVAL DYNAMICS

Theodore A. Deligiannis^1*, Tyler M. Wiles¹, Seung Kyeom Kim¹, Nick Stergiou^1,2, Aaron D. Likens¹

¹Department of Biomechanics, University of Nebraska at Omaha

*Corresponding author’s email: tdeligiannis@unomaha.edu

Presentation preference: Podium

Traditional gait metrics, like speed, cadence, coordination, as well as non-linear measures, appear related to Timed Up and Go (TUG). Timed Up and Go is a clinical measurement related to physical, socioeconomic, and psychological parameters, as well as overall health. TUG can be used as a health …

Human Blood Cell Isolation: The Critical First Step In Our Laboratory’S Immunobiology Experimental Protocals, Victor Rivero

UNO Student Research and Creative Activity Fair

HUMAN BLOOD CELL ISOLATION: THE CRITICAL FIRST STEP IN OUR LABORATORY’S IMMUNOBIOLOGY EXPERIMENTAL PROTOCALS

Victor Rivero1 Paul W. Denton1, vrivero@unomaha.edu

1Department of Biology, University of Nebraska at Omaha, Omaha, NE

The Denton Immunobiology Laboratory focuses on enhancing human natural killer (NK) cell killing capabilities, particularly in the context of combating cancer. NK cells are immune cells that have the ability to kill diseased cells via two mechanisms: direct killing, and antibody-dependent cell-mediated cytotoxicity (ADCC). We recently published our novel approach to testing both methods of killing by using NK cells derived from the same human donor. Our testing approach allows …

Every Little Thing, Dereck Daschke

Journal of Religion & Film

This is a film review of Every LIttle Thing (2024), directed by Sally Aitken.

Love Machina, John C. Lyden

Journal of Religion & Film

This is a film review of Love Machina (2024), directed by Peter Sillen.

Nonan Gaitprint: An Imu Gait Database Of Healthy Young Adults, Tyler M. Wiles, Madhur Mangalam, Joel H. Sommerfeld, Seung Kyeom Kim, Kolby J. Brink, Anaëlle Charles, Alli Grunkemeyer, Marilena Kalaitzi Manifrenti, Spyridon Mastorakis, Nicholas Stergiou, Aaron Likens

Journal Articles

An ongoing thrust of research focused on human gait pertains to identifying individuals based on gait patterns. However, no existing gait database supports modeling efforts to assess gait patterns unique to individuals. Hence, we introduce the Nonlinear Analysis Core (NONAN) GaitPrint database containing whole body kinematics and foot placement during self-paced overground walking on a 200-meter looping indoor track. Noraxon Ultium MotionTM inertial measurement unit (IMU) sensors sampled the motion of 35 healthy young adults (19–35 years old; 18 men and 17 women; mean ± 1 s.d. age: 24.6 ± 2.7 years; height: 1.73 ± 0.78 m; body mass: …

Development And Implementation Of Natural Killer Cell Simultaneous Adcc And Direct Killing Assay, Maia M.C. Bennett-Boehm, Anna Mahr, Alexander Regan, Sean T. Hartwell, Isabelle Weber, Arriana Blackmon, Cami Bisson, Angela Ngoc Truong, Bella Circo, Jaden Nienhueser, Donald Rogers, Nathan Booher, Nithya Rajagopalan, Jacob W.S. Martens, Paul Denton

Biology Faculty Publications

Assays to quantify natural killer (NK) cell killing efficacy have traditionally focused on assessing either direct killing or antibody dependent cell-mediated cytotoxicity (ADCC) independently. Due to the probability that immunotherapeutic interventions affect NK cell-mediated direct killing and NK cell-mediated ADCC differently, we developed an assay with the capacity to measure NK cell-mediated direct killing and ADCC simultaneously with cells from the same human donor. Specifically, this design allows for a single NK cell population to be split into several experimental conditions (e.g., direct killing, ADCC), thus controlling for potential confounders associated with human-to-human variation when assessing immunotherapy impacts. Our Natural …

Quantifying Non-Gaussian Intermittent Fluctuations In Physiology: Multiscale Probability Density Function Analysis Using The Savitzky-Golay Detrending, Madhur Mangalam, Damian G. Kelty-Stephen, Eiichi Watanabe, Ken Kiyono

Journal Articles

When measuring physiological data, the central limit theorem typically implies a consistent variance, resulting in data that closely follow a Gaussian distribution. However, physiological measurements often deviate from this expectation, increasing variance due to nonlinear correlations across various scales. The challenge lies in testing these tails, which comprise only rare and extreme values. We introduce multiscale probability density function (PDF) analysis, a method that estimates this non-Gaussianity parameter for physiological fluctuations in each of multiple timescales. We gain valuable insights into the observed distributions with heavier tails and nonlinear correlations by exploring the relationship between non-Gaussianity and logarithmic scale. To …

Multifractality In Stride-To-Stride Variations Reveals That Walking Involves More Movement Tuning And Adjusting Than Running, Taylor J. Wilson, Madhur Mangalam, Nicholas Stergiou, Aaron Likens

Journal Articles

Introduction: The seemingly periodic human gait exhibits stride-to-stride variations as it adapts to the changing task constraints. The optimal movement variability hypothesis (OMVH) states that healthy stride-to-stride variations exhibit “fractality”—a specific temporal structure in consecutive strides that are ordered, stable but also variable, and adaptable. Previous research has primarily focused on a single fractality measure, “monofractality.” However, this measure can vary across time; strideto-stride variations can show “multifractality.” Greater multifractality in stride-tostride variations would highlight the ability to tune and adjust movements more.

Methods: We investigated monofractality and multifractality in a cohort of eight healthy adults during self-paced walking and …

Impact Of A Tlr9 Agonist And Broadly Neutralizing Antibodies On Hiv-1 Persistence: The Randomized Phase 2a Titan Trial, Jesper D. Gunst, Jesper Falkesgaard Højen, Marie H. Pahus, Miriam Rosás-Umbert, Birgitte Stiksrud, James Mcmahon, Paul Denton, Henrik Nielsen, Isik S. Johansen, Thomas Benfield, Steffen Leth, Jan Gerstoft, Lars Østergaard, Mariane H. Schleimann, Rikke Olesen, Henrik Støvring, Line K. Vibholm, Nia Weis, Anne M. Dyrhol-Riise, Karen B.H. Pedersen, Jillian S.Y. Lau, Dennis C. Copertino, Noemi Linden, Tan T. Huynh, Victor Ramos, Rl Brad Jones, Sharon R. Lewin, Martin Tolstrup, Thomas A. Rasmussen, Michel C. Nussenzweig, Marina Caskey, Dag Henrik Reikvam, Ole Schmeltz Søgaard

Biology Faculty Publications

Inducing antiretroviral therapy (ART)-free virological control is a critical step toward a human immunodeficiency virus type 1 (HIV-1) cure. In this phase 2a, placebo-controlled, double-blinded trial, 43 people (85% males) with HIV-1 on ART were randomized to (1) placebo/placebo, (2) lefitolimod (TLR9 agonist)/placebo, (3) placebo/broadly neutralizing anti-HIV-1 antibodies (bNAbs) or (4) lefitolimod/bNAb. ART interruption (ATI) started at week 3. Lefitolimod was administered once weekly for the first 8 weeks, and bNAbs were administered twice, 1 d before and 3 weeks after ATI. The primary endpoint was time to loss of virologic control after ATI. The median delay in time to …

Temporal Organization Of Stride-To-Stride Variations Contradicts Predictive Models For Sensorimotor Control Of Footfalls During Walking, Madhur Mangalam, Damian G. Kelty-Stephen, Joel H. Sommerfeld, Nicholas Stergiou, Aaron Likens

Journal Articles

Walking exhibits stride-to-stride variations. Given ongoing perturbations, these variations critically support continuous adaptations between the goal-directed organism and its surroundings. Here, we report that stride-to-stride variations during self-paced overground walking show cascade-like intermittency—stride intervals become uneven because stride intervals of different sizes interact and do not simply balance each other. Moreover, even when synchronizing footfalls with visual cues with variable timing of presentation, asynchrony in the timings of the cue and footfall shows cascade-like intermittency. This evidence conflicts with theories about the sensorimotor control of walking, according to which internal predictive models correct asynchrony in the timings of the cue …

Assessing Student Participation In Cardio Related Activities While Enrolled In Fitness For Living, Preston Anderson

Theses/Capstones/Creative Projects

This capstone project investigated student participation in the KINS 1800: Fitness for Living course offered in the Health and Kinesiology College at the University of Nebraska at Omaha (UNO). The Fitness for Living Lab course aims to educate the students on different types of workouts to encourage the students to try different workouts/exercises throughout the semester long course.

During the spring 2023 semester I administered a 10-item pre and post survey to determine if students would partake in cardio activities that they were exposed to in the Fitness for Living course. Following analysis of the data it was noted that …

Ergodic Characterization Of Nonergodic Anomalous Diffusion Processes, Madhur Mangalam, Ralf Metzler, Damian G. Kelty-Stephen

Journal Articles

Anomalous diffusion in various complex systems abounds in nature and spans multiple space and time scales. Canonical characterization techniques that rely upon mean squared displacement break down for nonergodic processes, making it challenging to characterize anomalous diffusion from an individual time-series measurement. Nonergodicity reigns when the time-averaged mean square displacement differs from the ensemble-averaged mean squared displacement even in the limit of long measurement series. In these cases, the typical theoretical results for ensemble averages cannot be used to understand and interpret data acquired from time averages. The difficulty then lies in obtaining statistical descriptors of the measured diffusion process …

School Structure Differences And Physical Activity Of Youth: A Case Study Of Four Rural Communities, Jillian L. Kilty

Health and Kinesiology Theses, Dissertations, and Student Creative Activity

Purpose: To describe youth physical activity (PA) and participation in organized activities within school systems adopting different organizational structures. Design: Cross-sectional case-study of the Wellscapes Project with baseline data from Wave 1 (Fall 2018) and Wave 2 (Fall 2021). Setting: Four rural Midwestern communities. Sample: A total of 507 fourth, fifth, and sixth graders participated and were used in analyses (n = 156 fourth graders; n =189 fifth graders; n = 162 sixth graders). Two communities followed an elementary school model. The other two communities followed a middle school model. Measures: Students completed the Youth Activity Profile (YAP), an online …

The Study Of A Generalized Fitness Education Program's Effect On Personality Traits, Garrett Schliep

Theses/Capstones/Creative Projects

This longitudinal study was to measure the influence of a generalized fitness education program on the percentile of individuals’ personality traits in college aged individuals. The personality traits are those determined in the Five Factor Model of Personality. The Five Factor Model of Personality is a beneficial tool used in exercise psychology that refers to a broad domain of personality traits often referred to as the “Big Five”. The findings reject the hypothesis that a fitness education program would shift an individual’s personality a statistically significant amount.

Correlation Between Stress Coping Styles And Genetic Variation In The Adenosine Pathway Of Zebrafish, Sydney Klucas

Theses/Capstones/Creative Projects

Anxiety is a common mental disorder and is caused in part by dysregulation of neurotransmitter systems. Specifically, the adenosine pathway has been correlated with anxiety disorders, but it is not yet known if genetic differences in this pathway are responsible for anxiety-related behavior. In this study, I used bioinformatic analyses (EdgeR and WGCNA) to investigate an RNA-sequencing dataset to assess differences in differential gene expression and gene co-expression networks within the adenosine pathway of zebrafish with different stress coping styles. These analyses included three brain regions: basolateral amygdala, hippocampus, and habenula. The differential gene expression analysis demonstrated that the adenosine …

Cytotoxic Effects Of Bpa And Dehp On Human Epithelial Cells, Noah Stickrod

Theses/Capstones/Creative Projects

Endocrine disrupting chemicals (EDCs) are substances in the environment, food sources, personal care products, and manufactured products that interfere with the normal function of the body’s endocrine system. Most humans are exposed to some amount of these chemicals from many different sources, such as the air we breathe, food we eat, and the water we drink. EDCs can also permeate through the skin. The endocrine system is important because it works with other systems in the body to ensure healthy development and function throughout life. EDCs interfere with the way our body’s hormones work. Some EDCs can mimic natural hormones …

Gaze During Turning In Older Adults, Kalina Mavrov

Theses/Capstones/Creative Projects

To navigate complex environments, our gaze needs to attenuate to locomotor tasks, such as walking and turning. Shifting gaze (i.e., rotation of the eyes and head), is important when moving through different environments. The aim of this study was to compare gaze anticipation during clinical tests and complex real-world locomotion. I hypothesized that older adults would shift their gaze in a new heading direction in anticipation of turns. I further predicted increased gaze anticipation in tasks that have a high demand for spatial orientation. Participants were asked to complete clinical tasks consisting of a 2-minute walk, figure-8, and 360-turning in …

Accelerometry-Based Analysis Of Postural Sway In Parkinson's Disease Patients With Levodopa-Induced Dyskinesia, Chandler Brock

UNO Student Research and Creative Activity Fair

Parkinson’s disease (PD) is a progressive neurodegenerative disorder, with patient numbers projected to double to 12 million in the next 20 years. Levodopa-induced dyskinesia (LID) is a major problem associated with the long-term use of levodopa for symptomatic treatment of PD. These involuntary movements can become disabling and may interfere with quality of life. Our prior research showed that PD w/ LID were less stable while standing (i.e., increased postural sway) and had a higher incidence of falls. The aim of this study is to determine if postural sway properties are altered by LID via decomposing the sway signal. We …