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Non-Invasive Focused Ultrasound Treatment To Improve Depressive-Like Behavior In A Hemi-Parkinsonian Rat Model, Huda Rehman
Non-Invasive Focused Ultrasound Treatment To Improve Depressive-Like Behavior In A Hemi-Parkinsonian Rat Model, Huda Rehman
ALL - Honors Theses
Parkinson’s disease (PD) is a neurodegenerative disorder in which there is a depletion of dopaminergic neurons in the substantia nigra pars compacta (SNc). Symptoms include those that are motor, such as tremors and rigidity, along with symptoms that are nonmotor, such as depression. Depression in Parkinson’s disease is seen in up to 50% of PD patients, and is often treated with traditional antidepressants, with varying levels of efficacy. In this research project, focused ultrasound was used as a non-pharmacological and non-invasive treatment to improve depressive-like behavior in a hemi-Parkinsonian rat model. To induce a hemi-Parkinsonian phenotype in rats, stereotaxic surgeries …
Determining The Effect Of Absorbents And Soybean Systems On Pfas Precursor Transformation, Tamia Wellington
Determining The Effect Of Absorbents And Soybean Systems On Pfas Precursor Transformation, Tamia Wellington
Biological Sciences
PFAS (Per- and polyfluoroalkyl substances) refers to a class of chemicals that consist of long-chains of carbon bonded to fluorine. These are chemicals that have many industrial uses, from cosmetics to industrial fire-fighting foam. Since PFAS is so widely used and available, it has become increasingly present in water and soil, resulting in its uptake and bioaccumulation in plants. This study focused on the impacts of powdered activated carbon (PAC) and montmorillonite on the PFOS precursor N-ethyl perfluorooctane sulfonamido acetic acid (N-EtFOSAA), in soil, and in plants grown in that soil. This study focused on spiking soil with PFAS chemicals, …
Determining The Functional Roles Of Short Open Reading Frames In Mycobacterium Smegmatis, Lauren Fox
Determining The Functional Roles Of Short Open Reading Frames In Mycobacterium Smegmatis, Lauren Fox
Biological Sciences
The study examines the roles of a subset of short open reading frames (sORFs) in M. smegmatis using targeted mutagenesis and the subsequent examination of phenotypes associated with sORF mutation or overexpression. sORFs are defined as stretches of nucleic acid encoding a protein with a maximum of 50 amino acids. Genome annotation pipelines overlook sORFs encoding small proteins (sproteins) and, thus, sproteins in bacteria have gone unnoticed, even those that are expressed at levels similar to larger, well-described proteins. As a result of recent advances in ribosome profiling and mass spectrometry, hundreds of previously unannotated sORFs have been identified, increasing …
Quantification Of Fibrosis And Infiltrates In The Salivary Gland, Nikhita Kumar
Quantification Of Fibrosis And Infiltrates In The Salivary Gland, Nikhita Kumar
Biological Sciences
Fibrosis is characterized by the excessive buildup of extracellular matrix components (ECM) in tissues and organs in response to injury, chronic inflammation, auto immune disorders, and genetic alterations. Non-obese diabetic mice (NOD) are a model for Sjögren’s Syndrome. We hypothesize that NOD mice, which start to develop Sjögren’s-like disease at approximately 12 weeks of age are suffering from hyposalivation at least in part due to fibrosis of the submandibular salivary glands (SMGs). Fibrosis refers to the over-production of extracellular matrix proteins in the connective tissue compartment. To test this hypothesis 16-week-old mice were treated with either the fibrosis inhibitor Nintedanib …
Improvement Of Depressive-Like Behaviors In Hemi-Parkinsonian Rats With Non-Invasive Peripheral Focused Ultrasound Modulation, Hudy Berger
Biological Sciences
Parkinson’s disease (PD) is a chronic and progressive neurodegenerative disease with marked loss of dopaminergic neurons in the substantia nigra pars compacta (SNc). In PD, there are motor and non-motor symptoms. Depression, a non-motor symptom, is seen in 40% of the patients with PD which decreases their quality of life. For treatment-resistant depression, vagus nerve stimulation (VNS) has been shown to improve depression. Here, non-invasive focused ultrasound (FUS) is investigated as a therapeutic to improve depression-like behavior by targeting the celiac plexus since it is innervated by the vagus nerve. FUS was chosen due to its parallel mechanism to VNS. …
Investigating The Effects Of Vitamin K1 And K2 On The Triple Negative Breast Cancer Cell Line Sum159pt, Monica E. Alexander
Investigating The Effects Of Vitamin K1 And K2 On The Triple Negative Breast Cancer Cell Line Sum159pt, Monica E. Alexander
Biological Sciences
Vitamin K serves as a cofactor in the post-translational carboxylation of glutamate to γ-carboxyglutamate (GLA) by the enzyme gamma-glutamyl carboxylase (GGCX). Vitamin K epoxide reductases (VKOR) then oxidize the vitamin K to regenerate the compound’s reduced form. While most of the known γcarboxylated proteins function in coagulation and bone homeostasis, the presence of GGCX and VKOR in a wide variety of tissues suggests that the vitamin and its pathway has more extensive physiological roles. Our previous research has assessed the expression of the vitamin K pathway in triple negative breast cancer cells (TNBCs) and compared the effects of vitamin K1 …
Progress Towards Peptide And Protein Synthesis Involving Thiol Moieties, Ryan Lo Chen
Progress Towards Peptide And Protein Synthesis Involving Thiol Moieties, Ryan Lo Chen
Biological Sciences
Polypeptides and proteins play crucial roles in biological systems, including enzymatic activity regulation, molecule transport, and cell and tissue structural support. Amino acids are the building blocks of peptides and proteins, and peptides can act as hormones, neurotransmitters, and enzymes. Proteins are made up of one or more polypeptide chains and have diverse functions such as catalyzing chemical reactions, transporting molecules, and providing structural support. Protein preparation can be done biologically by expression systems or isolation and purification from natural sources. While these methods have their advantages, they also have their shortcomings. Chemical synthesis is an alternative method that allows …
Characterization Of Protein-Protein Interactions In Quorum-Sensing Receptors From Gram Negative Bacteria, Jessica Schuh
Characterization Of Protein-Protein Interactions In Quorum-Sensing Receptors From Gram Negative Bacteria, Jessica Schuh
Biological Sciences
Pseudomonas aeruginosa is a bacterium capable of causing disease in immunocompromised individuals and individuals with underlying lung disorders. It controls the expression of a subset of its genes through quorum sensing, which is a cell-cell communication system involved in mediating the transition from individual to group behaviors. Group behaviors are cellular processes undertaken by the cell that are beneficial to the collective, such as biofilm formation – a key component of pathogenesis in the human lung. They rely on the production, accumulation, detection, and response to signal molecules called autoinducers. There are two acyl-homoserine lactone autoinducer quorum sensing systems in …
A Review Of The Forensic Applications For The Analysis Of Biological Fluids Using Fluorescence Spectroscopy, Raghavi Patel
A Review Of The Forensic Applications For The Analysis Of Biological Fluids Using Fluorescence Spectroscopy, Raghavi Patel
Biological Sciences
The use of science to compile information to assist in solving crimes is known as forensic science. Numerous bodily fluids, such as blood, semen, saliva, and vaginal fluid, are frequently discovered at crime scenes. This makes it crucial for forensic scientists to use analytical methods to examine these fluids to learn more about the crime, including the offender, victim, time since deposition, and other details. Although there are many techniques, the focus of this paper is fluorescence spectroscopy. Fluorescence spectroscopy is a rapid, inexpensive, and non-destructive technique used for forensic examination (Weber & Lednev, 2022). Fluorescence spectroscopy can be used …
Neuronal Glutamate Transporters Modulate Reward-Based Behaviors In Mice, Corey Nilon
Neuronal Glutamate Transporters Modulate Reward-Based Behaviors In Mice, Corey Nilon
Biological Sciences
Obsessive-compulsive disorder (OCD) is a neuropsychiatric disorder characterized by uncontrollable, intrusive thoughts (obsessions), restlessness, and repetitive behaviors (compulsions). One of the genes associated with OCD encodes the neuronal glutamate transporter EAAC1. EAAC1 is expressed in the striatum, the main input nucleus of the basal ganglia, implicated with reward, which shows patterns of hyperactivity in patients with OCD. What remains unknown is how EAAC1 contributes to the different behavioral abnormalities of OCD. To address this question, we performed a set of behavioral tests in EAAC1-/- mice. Specifically, we asked how the execution of repetitive reward-based behaviors changed in these mice. Our …
Characterizing And Epitope Mapping Single-Domain Antibodies On Borrelia Burgdorferi Protein Ospa, Saiful Basir
Characterizing And Epitope Mapping Single-Domain Antibodies On Borrelia Burgdorferi Protein Ospa, Saiful Basir
Legacy Theses & Dissertations (2009 - 2024)
Epitope mapping a protein that enables pathogenesis is crucial for the development of therapies and prophylactics that can inhibit the pathogen’s function and its transmission of disease. The lipoprotein OspA enables Lyme Disease etiologic pathogen, Borrelia burgdorferi, to inhabit the tick midgut until transmission occurs. Anti-OspA mAbs and their smaller VHH counterparts are highly specific and tailored to bind proteins such as OspA, reproducibly, at established binding sites or epitopes. Previous studies found an array of mAbs that successfully bound OspA and have already been used in epitope mapping. To our knowledge, this is the first use of VHHs in …
Evaluating The Health Impacts Of Fruit And Vegetable Intake At The Individual Level And Food Pantry Level Among Food Pantry Users, Jiacheng Chen
Evaluating The Health Impacts Of Fruit And Vegetable Intake At The Individual Level And Food Pantry Level Among Food Pantry Users, Jiacheng Chen
Legacy Theses & Dissertations (2009 - 2024)
Background: Chronic diseases impose heavy burdens on individuals and the healthcare system in the US. Many factors were found to be associated with chronic diseases, including demographics, family history, social environmental factors, and individual behavioral factors such as diet and physical activity. Among those factors, fruit and vegetable intake can have substantial health impacts via a variety of causal pathways. Fruit and vegetable (F&V) consumption is generally lower among individuals living in households experiencing food insecurity and rely on food assistance programs. Decreased F&V intake among food pantry users may negatively impact health. However, conducting quantitative analysis on this population …
Understanding SjöGren's Syndrome As A Systemic Autoimmune Disorder, Gaietchyne Chery
Understanding SjöGren's Syndrome As A Systemic Autoimmune Disorder, Gaietchyne Chery
Legacy Theses & Dissertations (2009 - 2024)
Sjögren’s syndrome is an autoimmune condition characterized by a dysfunction in the lachrymal and salivary glands which results in dry eyes and dry mouth. Since its first description in 1892, the disease is one of the most common autoimmune diseases after lupus erythematosus and rheumatoid arthritis in the United States. Despite its high prevalence in the general population, Sjögren’s syndrome remains hard to diagnose due to the wide range of symptoms associated with the disease that is also shared by other conditions. Furthermore, the mechanisms behind the pathogenesis are not properly understood even though multiple factors have been proposed to …
Vircy-Seq : A Protocol For Characterizing Viral Activity, Tyler James Dion
Vircy-Seq : A Protocol For Characterizing Viral Activity, Tyler James Dion
Legacy Theses & Dissertations (2009 - 2024)
The main purpose of pharmaceutical production is to produce safe effective medicine for patient use. In an effort to ensure patient safety constant surveillance for viruses takes place. The detection of a viral nucleic acid in a pharmaceutical production setting results in investigations to assess its infectious potential. This is an intensive, expensive process that entails many tests such as the observation of hemadsorption, cytopathic effects (CPE), and more. These tests are typically specific and only capture certain viruses, as factors like CPE can only be observed in some viral species. A new investigational method that is effective on all …
Development Of Nucleic Acid Diagnostics For Targeted And Non-Targeted Biosensing, Christopher William Smith
Development Of Nucleic Acid Diagnostics For Targeted And Non-Targeted Biosensing, Christopher William Smith
Legacy Theses & Dissertations (2009 - 2024)
The field of nucleic acid technology is rapidly expanding with new impactful discoveriesbeing made each year. Starting from the discovery of the double-helix structure, cloning, gene editing, polymerase chain reaction (PCR), CRISPR technology, and even the late mRNA vaccines; nucleic acid technology is at the forefront of improving medicine. Nucleic acid technology is extremely versatile due to its easy programmability, automated cheap synthesis, and even its catalog for numerous chemical modifications that can be used to alter structure stability. For example, the number of permutations that can be made with DNA just by altering the code for adenine (A), cytosine …
An Ims-Ms/Md Workflow For Determining Higher Order Structure And Dynamics Of Nucleic Acids, Rebecca D'Esposito
An Ims-Ms/Md Workflow For Determining Higher Order Structure And Dynamics Of Nucleic Acids, Rebecca D'Esposito
Legacy Theses & Dissertations (2009 - 2024)
Ion mobility spectrometry - mass spectrometry (IMS-MS) has potential for the investigation of structure and dynamics in large biopolymers, which will come to full fruition only with a firmer understanding of how to interpret the experimental data. Numerous studies have employed elements of nucleic acid (NA) secondary structure, such as duplexes and hairpins, to explore the relationships between structure, experimental conditions, and actual observations. When combined with molecular dynamics simulations (MDS), IMS-MS can be effectively employed to perform structural elucidation of biomolecules that are not readily amenable to established techniques employed for structural analysis.
Parallel Networks That Govern The Transcriptional Response To Stress, Serene Anne Durham
Parallel Networks That Govern The Transcriptional Response To Stress, Serene Anne Durham
Legacy Theses & Dissertations (2009 - 2024)
The transcription factor, p53, plays a pivotal role in the oversight of many stimulus-dependent pathways. Its ability to respond to a wide variety of cellular stress stimuli by activating a broad range of target genes has led it to be characterized as a stress-dependent transcription factor. Our research focuses on deconvoluting the varied transcriptional response to distinct stress signals in an attempt to define the regulatory strategies leading to gene activation after cell stress. We have found that distinct stress response networks, some of which are p53-independent, are converging at activation of a common set of target genes. Our data …
N6-Methyladenosine Rna Modifications In Myogenesis / Narrative Competence And Cognitive Mapping As A Culturally Sustaining Pedagogy In The Education Of Emergent Bilinguals, Marina Danielle Infantado
N6-Methyladenosine Rna Modifications In Myogenesis / Narrative Competence And Cognitive Mapping As A Culturally Sustaining Pedagogy In The Education Of Emergent Bilinguals, Marina Danielle Infantado
Legacy Theses & Dissertations (2009 - 2024)
Myogenesis involves skeletal muscle stem cells (MuSCs) that produce and regenerate skeletal muscle during regular growth and repair. However, when this system fails to function normally, it can lead to musculoskeletal diseases like Duchenne Muscular Dystrophy (DMD). Therefore, it is important to study the molecular mechanisms behind this developmental process in order to seek therapies and solutions for these types of diseases. Our interest lies in the field of epitranscriptomics, which focuses on post-transcriptional ribonucleic acid (RNA) modifications, particularly N6-methyladenosine (m6A), which involves the addition of a methyl group to the adenosine nucleotide, a process that is mediated by the …
Amyloid Fibril Formation And Polymorphism : A Critical Role Of Sulfur-Containing Amino Acid Residues, Tatiana Quiñones-Ruiz
Amyloid Fibril Formation And Polymorphism : A Critical Role Of Sulfur-Containing Amino Acid Residues, Tatiana Quiñones-Ruiz
Legacy Theses & Dissertations (2009 - 2024)
Protein aggregation that results in the formation of amyloid fibrils has been linked to many neurodegenerative disorders, including Alzheimer’s disease and Parkinson’s disease. The sulfur atoms in methionine (Met) and cysteine (Cys) residues of proteins can be readily oxidized, significantly affecting their properties. Oxidation of sulfur-containing amino acids has recently been shown to affect protein fibrillation. This work presents novel findings on Cys and Met redox reactions that are related to the formation of amyloid fibrils and on the polymorphism of a model fibrillogenic protein, hen egg white lysozyme (HEWL). Biophysical techniques including Raman spectroscopy, atomic force microscopy, electron paramagnetic …
Potentially Cis-Acting Super-Enhancer Long Noncoding Rnas As Biomarkers In Triple Negative Breast Cancer Progression, Kavipriya Kovai Palanivel
Potentially Cis-Acting Super-Enhancer Long Noncoding Rnas As Biomarkers In Triple Negative Breast Cancer Progression, Kavipriya Kovai Palanivel
Biological Sciences
Triple negative breast cancer (TNBC) is defined by the lack of estrogen receptors (ER), the lack of progesterone receptors (PR), and normal human epidermal growth factor 2 (HER2) protein levels in breast tumors. Therefore, TNBC is an aggressive type of breast cancer due to limited treatment options for patients. Determining genes that regulate the progression of tumors to TNBC may help develop new therapeutic techniques. Many super-enhancer long noncoding RNAs (SElncRNAs) are found to be differentially expressed in cancer cells. Super-enhancer activity heavily influences target gene transcription and is often augmented through cis-acting SE-lncRNA activity. Therefore, we hypothesize cis-acting SE-lncRNAs …
Optophysiology Of D. Melanogaster Neuromuscular Synapses Using Gcamp, Kavindra Dasrat
Optophysiology Of D. Melanogaster Neuromuscular Synapses Using Gcamp, Kavindra Dasrat
Biological Sciences
An activity-dependent increase in postsynaptic currents produced by a single vesicle of neurotransmitter is an important mechanism for synaptic plasticity in the central nervous system (CNS) and possibly involved in learning and memory. We have found a similar form of postsynaptic potentiation at the D. Melanogaster neuromuscular junction (NMJ) where a brief increase in impulse activity results in an increase in the amplitude of spontaneous miniature excitatory postsynaptic currents (mEPSCs), resulting in miniature excitatory postsynaptic potentials (mEPSPs). To visualize sites of postsynaptic potentiation along the synaptic terminal, we have used a Ca2+ indicator, GCaMP to observe postsynaptic Ca2+ signals. Since …
Toward Long Non-Coding Rna Detection Using Dna Nanoswitches, Ché-Doni Platt
Toward Long Non-Coding Rna Detection Using Dna Nanoswitches, Ché-Doni Platt
Biological Sciences
Long non-coding RNAs (lncRNAs) are transcripts that are over 200 nucleotides and do not encode proteins. LncRNAs are involved in a wide spectrum of biological processes ranging from cell proliferation, apoptosis, and nutrient sensing to cell differentiation. Further, lncRNAs have been reported to play an important role in a wide range of pathophysiological processes and are linked to diseases such as cancer. Thus, lncRNAs are becoming increasingly important as biomarkers to study biological and disease processes. In our work, we develop a DNA nanoswitch-based assay for detecting long RNAs. The DNA nanoswitch is a reconfigurable device that undergoes a conformational …
Emerging Roles Of Intragenic Enhancers In Gene Expression Of Gadd45a, Ekaterina Sazon
Emerging Roles Of Intragenic Enhancers In Gene Expression Of Gadd45a, Ekaterina Sazon
Biological Sciences
Non-coding regulatory elements in the eukaryotic genomic DNA, like enhancers and promoters, control gene expression. Transcription factors recognize and bind to DNA sequences within these regulatory regions and affect said regulatory elements. The extent and specific circumstances regarding transcription factors are long-standing questions in the field. In this thesis, we wanted to investigate how transcription factors work in a particular case of enhancers, known as intragenic enhancers. We designed reporter gene assays that looked at the well-studied model transcription factor p53’s regulation on its target gene GADD45A in basal and stress conditions. We determined that GADD45A is a p53-dependent gene, …
Using The Centrifuge Force Microscope As A Single-Molecule Technique For Studying Base-Stacking Interactions, Kevin Thomas
Using The Centrifuge Force Microscope As A Single-Molecule Technique For Studying Base-Stacking Interactions, Kevin Thomas
Biological Sciences
A wide range of single-molecule techniques has streamlined the process of applying and studying the effects of force on individual molecules. The centrifuge force microscope (CFM) is one such technique that allows for high-throughput single-molecule pulling experiments. It is relatively inexpensive and user-friendly, making it a suitable tool to study the effects of force on various biomolecular interactions. A benchtop centrifuge can be used to apply centrifugal force to molecular constructs that are tethered between a glass slide and a microsphere. Molecular events like bond dissociation result in the removal of the microsphere from the surface, which is observed by …
Circadian Modulation Of Astrocyte Morphology And Synaptic Transmission, Saad Ahmad
Circadian Modulation Of Astrocyte Morphology And Synaptic Transmission, Saad Ahmad
Biological Sciences
The circadian rhythm affects behavior and physiology in many animal species. Our previous work in mice showed that within the hippocampus, a subordinate circadian oscillator, astrocytes change their proximity to excitatory synapses. Here we obtain 3D reconstructions of biocytin-filled hippocampal astrocytes processed with protein retention expansion microscopy and imaged using two-photon laser scanning microscopy. Our findings identify the subtle changes in astrocyte morphologies that encode information about time-of-day in hippocampal astrocytes. These findings shed light on fundamental mechanisms that allow the function of hippocampal circuits to adapt to different times of the day. This will help us understand how structural …
The Role Of Angiocrine Factors In The Progression Of Salivary Disease, Owen Zon
The Role Of Angiocrine Factors In The Progression Of Salivary Disease, Owen Zon
Biological Sciences
In our blood vessels, cells called endothelial cells line the inside of veins and arteries. Due to the discovery that endothelial cells actually organogenesis during embryogenesis, our interest in these cells has increased to a new understanding of their functions in tissue repair and regeneration. These cells are no longer only considered passive modes of transport for bodily fluids and nutrients, but, with new research in the fibrosis of liver cells, are also considered to be key players in the repair of damaged organs and tissue. These cells release signals known as angiocrine factors that act in damaged areas of …
Investigating The Roles Of Rna Modification And Associated Enzymes On Stress Response And Connections To Human Diseases, Carrson Widen
Investigating The Roles Of Rna Modification And Associated Enzymes On Stress Response And Connections To Human Diseases, Carrson Widen
Biological Sciences
Transfer RNAs decode messenger RNA using anticodon – codon interactions to deliver specific amino acids to a growing peptide chain. Enzyme-catalyzed modifications on specific nucleotides on tRNAs increase their stability and help mediate the speed and fidelity of translation, giving these modifications regulatory potential. RNA modifications are catalyzed by writer enzymes, and I predicted that writers are important in the cellular responses to stress. I have evaluated the following E. coli modification enzyme mutants: miaA, mnmE, dtd, tmcA, lysU, yeaK, and iscS, from the corresponding Keio E. coli gene deletion library. Writer mutants were exposed to hydrogen peroxide to induce …
Characterization Of The Role Of The Replicase And Quasispecies Diversity In Flaviviral Evolution And Host Adaptation, Haley Caldwell
Characterization Of The Role Of The Replicase And Quasispecies Diversity In Flaviviral Evolution And Host Adaptation, Haley Caldwell
Legacy Theses & Dissertations (2009 - 2024)
Flaviviruses include several emerging and re-emerging arboviruses that cause millions of infections each year. Although relatively well-studied, much remains unknown regarding the mechanisms and means by which these viruses adapt to different hosts and rapidly alternate between hosts. Different aspects of flaviviral biology impact host switching, viral fitness, and the generation of viral diversity during genome replication by the NS3 and NS5 proteins. Together these factors may impact host plasticity.
An Interaction Between Centrosome Scaffold Proteins And A Retrotransposon Nucleocapsid Protein May Alter Asymmetric Centrosome Inheritance, Lanilei Doctora
An Interaction Between Centrosome Scaffold Proteins And A Retrotransposon Nucleocapsid Protein May Alter Asymmetric Centrosome Inheritance, Lanilei Doctora
Legacy Theses & Dissertations (2009 - 2024)
Nearly all eukaryotic genomes harbor mobile genetic elements known as retrotransposons. The mRNA ofthese elements undergo reverse transcription, yielding cDNA that is inserted in a new location in the host genome. We study Saccharomyces cerevisiae Ty1 elements, an active family of retrovirus-like retrotransposons, and the mechanism behind its regulation of spindle pole body (SPB) inheritance. Ty1 retromobility is activated during growth at 20˚C. In a manuscript that includes my work (Murphy et al., 2022, manuscript in preparation), we report that essential SPB proteins Nud1 and Spc42 are sequestered in the Ty1 retrosome, the site of Ty1 nucleocapsid assembly, in a …
Bioinformatics Analysis Of The Transcriptomic Profiles Of Duchenne Muscular Dystrophy Patients / Narrative Competence And Cognitive Mapping As A Culturally Sustaining Pedagogy In The Education Of Emergent Bilinguals, Bianca Gonzalez
Legacy Theses & Dissertations (2009 - 2024)
Duchenne Muscular Dystrophy (DMD) is a muscle-wasting disease that primarily affects boys characterized by loss of the dystrophin gene. Dystrophin is necessary for proper muscle growth, muscles that lack dystrophin are more prone to damage. In this thesis, a bioinformatics approach was used to evaluate the transcriptomic profiles of two datasets obtained from DMD patients. The two datasets were analyzed separately, as well as combined to identify differentially expressed genes. The first dataset, GSE6011, has 23 DMD samples and 14 controls. The second, GSE38417, consists of 16 DMD samples and six controls. Differential gene expression of both datasets combined found …